Method for treating sludge hydrolysate by fungi and recovering mycothalli

A hydrolyzate and fungus technology, which is applied in the pyrolysis treatment of sludge, fungi, and sludge treatment, etc., can solve the problems of low utilization value of recovered methane resources, etc.

Inactive Publication Date: 2020-01-07
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above sludge hydrolyzate treatment methods are based on the decomposition of organic matter by bacteria, and the resource utilization value of recovering methane during the treatment process is low, or the sludge hydrolyzate has not been utilized as a resource

Method used

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  • Method for treating sludge hydrolysate by fungi and recovering mycothalli
  • Method for treating sludge hydrolysate by fungi and recovering mycothalli
  • Method for treating sludge hydrolysate by fungi and recovering mycothalli

Examples

Experimental program
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Effect test

Embodiment 1

[0032] see figure 1 , the present embodiment provides a method of using fungi to treat sludge hydrolyzate and reclaim fungal cells. The specific steps are as follows: take the remaining sludge with a SS concentration (suspended solid concentration) of 15g / L and place it in an autoclave. Under the conditions of 160°C and 200rpm, thermally hydrolyze for 60 minutes, pour out the sludge hydrolyzate after the temperature drops to room temperature, and filter it with a glass fiber membrane to obtain the sludge hydrolyzate. Take the sludge hydrolyzate and place it under high-pressure steam sterilization at 116°C for 20 minutes. After cooling down to room temperature, inoculate Aspergillus niger in the sludge hydrolyzate at an inoculation amount of 5% volume ratio in the ultra-clean workbench, and then place it at 30°C. , 150rpm constant temperature shaking incubator culture. Samples were taken every 12 to 24 hours to measure the COD concentration and fungal cell concentration of the...

Embodiment 2

[0035] The steps of this embodiment are the same as those of Example 1, except that the inoculated strain is Rhizopus oryzae.

[0036] The COD concentration of the sludge hydrolyzate inoculated with Aspergillus niger and the COD concentration of the sludge hydrolyzate inoculated with Rhizopus oryzae were measured respectively in embodiment 1, the results are as follows image 3shown. The results showed that after the inoculation of Aspergillus niger and Rhizopus oryzae, the COD concentration of the sludge hydrolyzate decreased gradually, and the COD concentration reached stability at 120h and 60h respectively after inoculation of Aspergillus niger and Rhizopus oryzae, and the COD removal rate was 35% respectively and 22%, the experimental results show that the fungi can degrade the organic matter in the sludge hydrolyzate. The growth of fungi in sludge hydrolyzate is as follows: Figure 4 As shown, after the inoculation of Aspergillus niger and Rhizopus oryzae, the concentra...

Embodiment 3

[0039] Take the remaining sludge with SS concentration (suspended solid concentration) of 30g / L and place it in a high-pressure reactor, and heat hydrolyze it at 160°C and 200rpm for 60min. Fibrous membrane filtration to obtain sludge hydrolyzate. Take the sludge hydrolyzate and place it under high-pressure steam sterilization at 116°C for 20 minutes. After cooling down to room temperature, inoculate Aspergillus niger in the sludge hydrolyzate at an inoculation amount of 5% volume ratio in the ultra-clean workbench, and then place it at 30°C. , 150rpm constant temperature shaking incubator culture. Samples were taken every 12 to 24 hours to measure the COD concentration and fungal cell concentration of the sludge hydrolyzate, and the cultivation was stopped after reaching a stable value. The treated sludge hydrolyzate and fungal cells are separated by filtration, and the recovered mycelium is dispersed and reshaped to make the mycelia cross-linked together to prepare thin fil...

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Abstract

The invention discloses a method for treating sludge hydrolysate by fungi and recovering mycothalli. The method comprises the following steps: (1) preparing the sludge hydrolysate: carrying out pyrohydrolysis on sludge, carrying out solid-liquid separation, and taking a liquid part to obtain the sludge hydrolysate; (2) treatment of the sludge hydrolysate: sterilizing the sludge hydrolysate; (3) inoculation with fungi: inoculating the fungi into the solution sterilized in the step (2), and culturing; and (4) recycling of the mycothalli: after the culture is finished, separating the mycothalli and recycling the mycothalli. High-concentration organic matters in the sludge hydrolysate are removed by the decomposition effect of the fungi on the organic matters; meanwhile, the fungus strain takes the sludge hydrolysate as a culture medium, and mycelia are formed by growth and enrichment of the organic matters in the sludge hydrolysate, so that obtaining of the high-purity mycothalli and recycling of the subsequent mycelia can be facilitated, a recycling effect is achieved, and the method has a wide application prospect in the field of sludge treatment.

Description

technical field [0001] The invention relates to the technical field of sludge treatment, in particular to a method for treating sludge hydrolyzate with fungi and recovering fungal cells. Background technique [0002] With the continuous development of social economy, the amount of urban sewage treatment continues to increase, and the discharge of excess sludge increases accordingly. The safe disposal of excess sludge has become an urgent problem to be solved. Thermal hydrolysis technology is a sludge pretreatment technology that has received wide attention in recent years. After sludge is treated by thermal hydrolysis, sludge flocs disintegrate, microbial cells are broken, colloidal structure is destroyed, and intracellular water is released. The settling performance and dewatering performance of mud are improved. At the same time, the macromolecular organic matter is fully released and hydrolyzed into small molecular organic matter during the thermal hydrolysis of sludge, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/10C02F11/121C02F11/127C02F11/00C12N1/14
CPCC02F11/10C02F11/121C02F11/127C02F11/00C12N1/14C02F2303/06
Inventor 李晓岩夏雪温磊于悦林琳
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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