Soil-phosphorus biological-activating fungi inoculant and preparation method thereof

A fungal inoculant and bioactivation technology, applied in biochemical equipment and methods, microbe-based methods, chemical instruments and methods, etc., can solve problems such as unstable field application effects, poor soil adaptability, and loss of phosphorus-solubilizing genes. Achieving the effect of dissolving phosphorus to increase production, strong soil adaptability, and short production cycle

Inactive Publication Date: 2012-07-25
宋丹丹 +1
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Problems solved by technology

[0006] After the 1980s, many domestic units have successively developed compound phosphate-solubilizing bacteria preparations composed of various bacillus, but the effects of these bacteria agents in production are not ideal, mainly manifested in the unstable field application effect
In addition to the poor soil adaptability of these phosphorus-solubilizing bacteria, the main reason leading to the unstable application effect in the field is that the bacteria used in these phosphorus-solubilizing preparations are all phosphorus-solubilizing bacteria, and t

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  • Soil-phosphorus biological-activating fungi inoculant and preparation method thereof
  • Soil-phosphorus biological-activating fungi inoculant and preparation method thereof

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[0040] The soil phosphorus bioactivation fungal inoculant of the present invention and preparation method thereof, the strain used is the clonal strain JB201015 (Eupenicillium euglaucum anamorph JB201015) of the phosphate-solubilizing fungus Penicillium euglaucum anamorph JB201015. The strain will irreversibly lose the disadvantage of the phosphorus-solubilizing gene; in the field application, the strain is colonized in the rhizosphere of the crops through the method of seed dressing, and the rhizosphere exudates of the crops are used as carbon and nitrogen sources for growth and reproduction. The metabolic activity of phosphorus-dissolving fungi converts insoluble phosphate (PO 4 3 - ), converted into soluble phosphate (H 2 PO 4 - 、HPO 4 2- ) for crop uptake and utilization. It overcomes the shortcomings of the poor soil adaptability of the phosphate solubilizing bacteria in the past.

[0041] Technical principle:

[0042] Phosphorus in soil mainly exists in two form...

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Abstract

The invention relates to soil-phosphorus biological-activating fungi inoculant and a preparation method thereof; the preparation method comprises the following steps: taking a slant strain, cultivating the slant strain at 25 degrees centigrade for 1-2 days; eluting spores with 15mL of sterile water so as to prepare spore suspension, absorbing 10mL of spore suspension, inoculating the spore suspension into a conical flask containing 100mL of PD (potato dextrose) culture solution, carrying out shaking culture at 26 degrees centigrade for 3-5 days so as to obtain a liquid strain; inoculating the liquid strain in an amount of 15-20% into first-grade seed PD culture solution; carrying out cultivation at 27 degrees centigrade for 1-2 days; inoculating the first-grade seed PD culture solution in an amount of 15-20% into second-grade seed PD culture solution so as to obtain a second-grade liquid strain; and absorbing the second-liquid strain with sterile grass carbon according to a ratio of 1: 4, then, crushing so as to obtain spore powder.

Description

(1) Technical field [0001] The invention relates to a preparation method of a soil phosphorus bioactivation fungal inoculant, in particular to a preparation method of a phosphorus-dissolving fungal inoculant. (2) Background technology [0002] Phosphorus is the material basis of all life activities. It is not only a component element of biological genetic information carrier DNA, but more importantly, it is a component element of biological energy carrier ATP. Insufficient phosphorus supply, all life will lose its original brilliance. In the field of agricultural production, the insufficient supply of available phosphorus is still one of the main limiting factors of global crop production. From the perspective of our country, 74% of the cultivated land in the country is deficient in phosphorus due to soil phosphorus fixation. According to reports: since my country began to apply chemical phosphate fertilizers in the 1950s, the accumulated insoluble phosphorus stored in the...

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Application Information

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IPC IPC(8): C12N11/14C12N3/00C09K17/14C12R1/645C09K101/00
Inventor 崔振
Owner 宋丹丹
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