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Method for improving spore yield of fungi

A technology of fungal spores and fungi, which is applied in the field of increasing the yield of fungal spores, can solve problems such as being unsuitable for commercial production, and achieve the effect of increasing the yield of spores

Inactive Publication Date: 2011-11-23
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has certain defects and is not suitable for commercial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Alternaria ( Arternaria alternata ) mycelia were inoculated on 20 ml of potato medium, and cultured at 25°C for 5-10 days; sprayed 500 μl of potassium permanganate solution with a concentration (mass) of 0.5% on the surface of Alternaria vegetative bodies cultured for 5-10 days; Blow Alternaria vegetative bodies treated with potassium permanganate solution for 10 minutes with sterile wind at a wind speed of 0.6 m / s; continue to cultivate Alternaria at 25°C for 3 days, and collect spores.

[0021] The same 20 ml potato culture medium was used as the control, which was cultured at 25°C without treatment with 0.5% potassium permanganate solution.

[0022] The conidia production of Alternaria treated with potassium permanganate solution was 9×10 7 / cm 2 , the conidia yield of the control was 2×10 7 / cm 2 . The conidia production of Alternaria treated with 0.5% potassium permanganate solution was 4.5 times that of the control.

Embodiment 2

[0024] Alternaria ( Arternaria alternata ) mycelia were inoculated on 20 ml potato medium, and cultured at 25°C for 5-10 days; sprayed 500 μl of hydrogen peroxide with a concentration of 0.2 % (w / v) on the surface of Alternaria vegetative bodies cultured for 5-10 days; The sterile wind with a wind speed of m / s blows the Alternaria vegetative bodies treated with hydrogen peroxide for 10 minutes; then continue to cultivate Alternaria for 3 days, and collect the spores.

[0025] As a control, the same 20 ml potato medium was used, which had been cultured at 25°C without 0.2% hydrogen peroxide treatment.

[0026] The conidia production of Alternaria treated with hydrogen peroxide was 7×10 7 / cm 2 , the conidia yield of the control was 2×10 7 / cm 2 , the yield of Alternaria conidia treated with 0.2% hydrogen peroxide was 3.5 times that of the control.

Embodiment 3

[0028] Viridans viridans ( Gliocladium virens ) mycelia were inoculated on 20ml potato medium, and cultured at 25°C for 5-10 days; sprayed 500μl concentration (mass) of 0.5% potassium permanganate solution on the surface of Alternaria vegetative bodies cultured for 5-10 days; The aseptic wind with a wind speed of m / s blows the Myxomyces viridans treated with the potassium permanganate solution for 10 minutes; then continue to cultivate Alternaria for 3 days, and collect the spores.

[0029] As a control, the same 20ml potato medium was used, which was cultured at 25°C without treatment with 0.5% potassium permanganate solution.

[0030] The chlamydospore yield of the chlamydospores treated by 0.5% potassium permanganate solution was 2×10 6 / cm 2 , the chlamydospore yield of the control was 9×10 5 / cm 2 , 0.5% potassium permanganate solution treated the chlamydospore yield of the chlamydospores of the chlamydospores treated by 0.5% potassium permanganate solution was 2.2 t...

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PUM

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Abstract

The invention discloses a method for improving spore yield of fungi, which comprises: inoculating fungi onto a culture medium, culturing at a proper culture temperature for 5 to 10 days, spraying 500 microliters of strong oxidizer solution at a proper concentration onto the surface of a fungus nutrition body, and blowing the fungus nutrition body with sterile wind at a speed of 0.6m / s for 10 minutes; and continuing to culture the fungi at the same culture temperature for 3 days and collecting the spores generated by the fungi. In the method, a strong oxidizer is used to arouse certain stress in the fungi to promote the fungi to grow spores which are organs more adaptive to the environment, and thus, the spore yield of the fungi is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for increasing the yield of fungal spores. In the method, the vegetative hyphae of the fungi are treated with a strong oxidant of appropriate concentration, and a certain stress is produced on the fungus to promote the production of fungi with stronger adaptability to the environment. Organs—spores, so as to promote sporulation of fungi to increase their spore production. Background technique [0002] Fungal spores are generally more stable than their somatic cells and mycelia, and thus able to survive harsh environments, and are therefore widely used in the preparation of biocontrol or other commercial fungal product formulations (Shah FA, et al , Biol Control, 2007, 40, 246-252; Decal A, et al , Recent Res Dev Appl Microbiol & Biotechnol, 2003, 1, 161-175). In commercial production, it is often necessary to artificially cultivate fungi, but many fungi will weaken or even...

Claims

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Application Information

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IPC IPC(8): C12N3/00
Inventor 徐玲玲陶贵荣郭斌孟长军
Owner XIAN UNVERSITY OF ARTS & SCI
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