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Compound for treating and preventing diabetes and preparation method thereof

A compound and diabetes technology, which is applied in the field of compounds for the treatment and prevention of diabetes and its preparation, can solve the problems of high difficulty in collecting marine samples and incomplete expression of metabolites, achieve good inhibitory effect, prevent and treat diabetes, and increase yield Effect

Inactive Publication Date: 2019-07-23
嘉兴市爵拓科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of marine samples is difficult, and only less than 5% of marine microorganisms have been isolated under routine laboratory conditions, and even the obtained microorganisms cannot fully express all their biosynthetic pathways in the marine environment metabolite

Method used

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  • Compound for treating and preventing diabetes and preparation method thereof
  • Compound for treating and preventing diabetes and preparation method thereof
  • Compound for treating and preventing diabetes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of a compound for treating and preventing diabetes, comprising,

[0043] 1) Isolation and purification of Enteromorpha-derived fungi: the fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed successively with sterile seawater, 75% ethanol and sterile water, Then, the sample was crushed with a mortar, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol, and cultivated at 28 ° C until A single strain appeared, and the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as Aspergillus terreus OUCMDZ- 2739;

[0044] 2) Enteromorpha-derived fungi were inoculated in a fermenta...

Embodiment 2

[0047] A preparation method of a compound for treating and preventing diabetes, comprising,

[0048] 1) Isolation and purification of Enteromorpha-derived fungi: the fungal strain Aspergillus terreus OUCMDZ-2739 was isolated from Enteromorpha samples from Shilaoren Beach in Qingdao, and the Enteromorpha samples were washed successively with sterile seawater, 75% ethanol and sterile water, Then, the sample was crushed with a mortar, and then stored in PDA medium (containing 200 g of potato extract, 20 g of glucose, 15 g of agar, and 1 L of seawater per liter) containing 100 mg / mL chloramphenicol, and cultivated at 28 ° C until A single strain appeared, and the strain was transferred to another PDA medium and stored at 4°C. Through multiphase taxonomic studies, colony morphology and 18S rRNA sequence analysis, and establishment of strain development trees, it was identified as Aspergillus terreus OUCMDZ- 2739;

[0049] 2) Enteromorpha-derived fungi were inoculated in a fermenta...

Embodiment 3

[0057] In order to improve the resolution of the target compound, the further optimization scheme is:

[0058] During the HPLC purification, 60% MeOH-H 2 A solution of O, 0.35‰phenylphosphonic acid and 0.15% TFA was eluted. As a strong polar solvent, MeOH (methanol) can be dissolved in n-hexane / manghol or n-hexane / isopropanol solution. It is highly polar and protic, cheap and easy to purify. TFA (trifluoroacetic acid) is one of many organic A good solvent for compounds, trifluoroacetic acid in the mobile phase can improve peak shape, overcome peak broadening and tailing problems by interacting with hydrophobic bonded phases and residual polar surfaces in multiple modes; protonation can be used in target compounds , MeOH, phenylphosphonic acid and TFA in the above solution can play a positive role, can provide sufficient protons, so that the target compound and the stationary phase can undergo more sufficient adsorption and desorption exchange, and can shorten the peak time of...

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Abstract

The invention provides a compound for treating and preventing diabetes and a preparation method thereof, and belongs to the technical field of microbial drugs. A preparation method of a secondary metabolite comprises the following steps: separating and purifying enteromorpha-derived fungi; inoculating fermentation liquor containing TSA and glycyrrhetinic acid with the enteromorpha-derived fungi for fermentation; and extracting, separating and purifying the secondary metabolite of enteromorpha-derived fungi. A new use of the secondary metabolite of enteromorpha-derived fungi is a new application in the preparation of alpha-glucosidase inhibitors and / or medicaments for treating diabetes. The preparation method of the secondary metabolite can improve the yield of the secondary metabolite, promote the proliferation activity of the enteromorpha-derived fungi and improve the yield of secondary metabolite. The secondary metabolite has good inhibition effect on alpha-glucosidase, slows down the generation and absorption of glucose, prevents postprandial hyperglycemia, has good effect on preventing and treating diabetes, and is easy to be absorbed by organisms.

Description

technical field [0001] The invention belongs to the technical field of microbial medicines, and in particular relates to a compound for treating and preventing diabetes and a preparation method thereof. Background technique [0002] In the nearly one hundred years of research on terrestrial microorganisms, a large number of natural products with diverse chemical structures and significant biological activities have been discovered, which has greatly promoted the development of biotin drugs, such as: penicillin, vancomycin, streptomycin, etc. More and more attention to microorganisms naturally leads to repeated research on microorganisms, repeated development of known compounds, and a lower chance of discovering new compounds. Therefore, people began to pay attention to marine-derived microorganisms with larger microbial reserves and more diverse species. Due to their unique living environment (high pressure, high salt, low temperature, oligotrophic), marine microorganisms h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C07D407/14A61P3/10C12R1/66
CPCA61P3/10C07D407/14C12P17/162
Inventor 孙坤来张译文宫凯凯赵成英
Owner 嘉兴市爵拓科技有限公司
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