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Composition for embryonic stem cell culture and application thereof

A composition and epidermal cell technology, applied in the field of cytology, can solve the problems that the culture medium cannot achieve the effect

Inactive Publication Date: 2018-08-07
重庆斯德姆生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing media can not achieve the desired effect

Method used

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  • Composition for embryonic stem cell culture and application thereof
  • Composition for embryonic stem cell culture and application thereof
  • Composition for embryonic stem cell culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The compositions used for culturing embryonic stem cells provided in this embodiment include:

[0036]

Embodiment 2

[0038] The compositions used for culturing embryonic stem cells provided in this embodiment include:

[0039]

Embodiment 3

[0041] The compositions used for culturing embryonic stem cells provided in this embodiment include:

[0042]

[0043]

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PUM

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Abstract

The invention provides a composition for embryonic stem cell culture. The composition can ensure embryonic stem cells to be in an undifferentiation state for a long time and to have unlimited multiplication capacity. The composition comprises leukaema inhibitory factor, transforming growth factor beta 1, epidermal cell growth factor, basic fibroblast growth factor, Rho kinase inhibitor Y27632, MEKinhibitor and bone morphogenetic protein inhibitor Noggin. The invention further provides cell culture liquid comprising the composition and application thereof.

Description

technical field [0001] The invention relates to the field of cytology, in particular to a composition for culturing embryonic stem cells and its application. Background technique [0002] The successful isolation and culture of human embryonic stem cells is an important progress in today's life sciences. It is isolated from the inner cell mass of early human preimplantation embryos and can maintain undifferentiated and continuously proliferate under appropriate conditions in vitro. kind of stem cells. Theoretically, it has the potential to differentiate into all cell tissues of the body, and is called "seed cell". People can use different culture conditions to induce differentiation of embryonic stem cells to produce a large number of different types of cells as cell donors for cell transplantation, tissue replacement and even organ cloning, providing unlimited cell sources for the future treatment of many intractable diseases in humans. Therefore, it has broad prospects i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735
CPCC12N5/0606C12N2501/11C12N2501/115C12N2501/15C12N2501/155C12N2501/235C12N2501/405
Inventor 王健熊华强宋彩霞胡小东
Owner 重庆斯德姆生物技术有限公司
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