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Preparation and seeding method of a kind of ectomycorrhizal fungus granule

A technology of ectomycorrhizal fungus and granules, which can be applied in botany equipment and methods, horticulture, agriculture, etc., and can solve the problem that input and output are not suitable for large-scale promotion and application, not suitable for large-scale promotion and application, and reduce the activity of bacteria, etc. problem, to achieve the effects of simple production and sowing methods, good water retention, and easy operation

Active Publication Date: 2022-05-03
ZUNYI INST OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, ectomycorrhizal edible and medicinal fungi cannot be reproduced under purely artificial conditions. In the past, ectomycorrhizal fungus strains were sprayed on the host rhizosphere with a sprayer, which is not suitable for large-scale cultivation. There are two main factors for large-scale promotion and application: (1) high labor intensity, and the input-output ratio is not suitable for large-scale promotion and application; (2) in case of drought, the activity of bacteria will be greatly reduced
According to previous research data, they were all limited to small-scale experiments, and many factors such as strain application and management were not considered. The above two factors are a major bottleneck for the large-scale promotion and application of ectomycorrhizal edible and medicinal fungi.

Method used

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  • Preparation and seeding method of a kind of ectomycorrhizal fungus granule
  • Preparation and seeding method of a kind of ectomycorrhizal fungus granule
  • Preparation and seeding method of a kind of ectomycorrhizal fungus granule

Examples

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Embodiment 1

[0092] A preparation method for ectomycorrhizal fungus granule strains, comprising the following steps:

[0093] (1) placing the ectomycorrhizal bacteria in the container, stirring and breaking the mycelium to obtain the ectomycorrhizal bacteria liquid; the stirring speed is 250r / min, and the time is 3min;

[0094] (2) The nutrient solution is added in the ectomycorrhizal solution of step (1) and stirred evenly to obtain a mixed solution; the nutrient solution and the ectomycorrhizal solution are mixed according to equal volumes; the nutrient solution consists of the following Made from raw materials in parts by weight: 20 parts of raw corn flour, 10 parts of glucose, 0.3 parts of calcium chloride, 0.3 parts of sodium chloride, 1 part of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, 0.05 parts of VB1, potassium indole butyrate 0.5 parts; the preparation method is: add raw corn flour to water, heat and stir at 60°C to dissolve the raw corn flour into corn liquid...

Embodiment 2

[0101] A preparation method for ectomycorrhizal fungus granule strains, comprising the following steps:

[0102] (1) placing the ectomycorrhizal bacteria in the container, stirring and breaking the mycelium to obtain the ectomycorrhizal bacteria liquid; the stirring speed is 300r / min, and the time is 5min;

[0103] (2) The nutrient solution is added in the ectomycorrhizal solution of step (1) and stirred evenly to obtain a mixed solution; the nutrient solution and the ectomycorrhizal solution are mixed according to equal volumes; the nutrient solution consists of the following Made from raw materials in parts by weight: 50 parts of raw corn flour, 40 parts of glucose, 1 part of calcium chloride, 1 part of sodium chloride, 1-3.5 parts of magnesium sulfate, 5 parts of potassium dihydrogen phosphate, 0.15 parts of VB1, indole 2 parts potassium acid potassium; the preparation method is as follows: add raw corn flour to water, heat and stir at 100°C to dissolve the raw corn flour i...

Embodiment 3

[0110] A preparation method for ectomycorrhizal fungus granule strains, comprising the following steps:

[0111] (1) placing the ectomycorrhizal bacteria in the container, stirring and breaking the mycelium to obtain the ectomycorrhizal bacteria liquid; the stirring speed is 260r / min, and the time is 3.5min;

[0112] (2) The nutrient solution is added in the ectomycorrhizal solution of step (1) and stirred evenly to obtain a mixed solution; the nutrient solution and the ectomycorrhizal solution are mixed according to equal volumes; the nutrient solution consists of the following Made from raw materials in parts by weight: 30 parts of raw corn flour, 20 parts of glucose, 0.5 parts of calcium chloride, 0.5 parts of sodium chloride, 1.5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate, 0.08 parts of VB1, potassium indole butyrate 1.0 parts; the preparation method is: add raw corn flour to water, heat and stir at 70°C to dissolve the raw corn flour into corn l...

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Abstract

The invention discloses a preparation of ectomycorrhizal fungus granules, which comprises the following steps: (1) stirring and smashing the mycelium of ectomycorrhizal fungi to obtain ectomycorrhizal fungus liquid; Add the liquid into the ectomycorrhizal liquid of step (1) to obtain a mixed liquid; (3) slowly add the mixed liquid in step (2) into polyacrylamide, continue stirring until the mixed liquid is completely absorbed, and then add wheat bran powder and continue stirring to obtain the ectomycorrhizal fungus granule strain; the sowing method adopts root cutting on an inclined plane or root cutting in a hole shape. The ectomycorrhizal fungus granules strains of the present application have improved the problems of the prior art, which are limited by the distribution of the target mycorrhizal fungi, and the labor intensity of sowing is high, and the problems of being greatly affected by the weather, and the raw materials used are simple and easy to obtain, the preparation method is simple, and the operation is easy. The method is simple and convenient, reduces the production cost, improves the economic benefit, and is suitable for popularization and application in a large area.

Description

technical field [0001] The invention belongs to the technical field of edible fungus strain seed production, in particular to a method for preparing and sowing ectomycorrhizal fungus granule strains. Background technique [0002] Many macrofungi form symbiotic relationships with the roots of higher plants, which we call ectomycorrhizae. There are extremely rich ectomycorrhizal fungi in China, and more than 600 species are known, such as Russula, Lactarum, Amanita, Boletus, and Scleroderma scleroderma in the agaric family Truffles of the genus Beanbag and Ascomycetes. The research and application of ectomycorrhizal fungi are of great significance for the development of forestry seedlings, the promotion of forest tree growth and development, and the greening of barren hills and mine waste land. Ectomycorrhizal fungi are mycorrhizal complexes formed by fungi and the roots of higher plants, and the metabolites of the host and mycorrhizal fungi operate bidirectionally through t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/50
CPCA01G18/50
Inventor 易筑刚
Owner ZUNYI INST OF FORESTRY
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