Efficient and practical castanea mollissima blume ectomycorrhiza soil pot inoculation system and application thereof

Abstract

The invention provides an efficient and practical castanea mollissima blume ectomycorrhiza soil pot inoculation system and an application thereof, and relates to the technical field of ectomycorrhizaculture. The inoculation system comprises a culture soil pot, a matrix, a solid culture medium with fungal hyphae and castanea mollissima blume seedlings, wherein the matrix is sterilized vermiculite;the castanea mollissima blume seedlings are planted in the culture soil pot with the vermiculite as the matrix; the solid culture medium with the fungal hyphae is placed around the root systems of the castanea mollissima blume seedlings for a circle and is placed at the position 3 cm away from the soil surface as much as possible; and the fungal hyphae on the solid culture medium make contact with the root systems of the castanea mollissima blume seedlings. By means of the inoculation system, the infection rate of the root systems of the castanea mollissima blume seedlings and fungi can be effectively increased, the generation time of ectomycorrhizae is shortened, the formation efficiency of the ectomycorrhizae of the castanea mollissima blume can be improved to 100% in the culture soil pot; and mycorrhizae of different plants are consistent.

Description

technical field [0001] The invention relates to the technical field of ectomycorrhizal culture, in particular to an efficient and practical chestnut ectomycorrhizal soil pot inoculation system and application thereof. Background technique [0002] Ectomycorrhizal (Ectomycorrhiza) is a symbiont established by the interaction between plants and ectophytic fungi. Usually, mycelium will wrap around the root system of the host plant to form a fungus mantle, such as the symbiosis of chestnuts and orange-yellow hard-skinned puffballs found in the field, which are clearly visible to the naked eye. Ectomycorrhizae in the form of white mantles. Ectomycorrhizae are commonly found in forest ecosystems in temperate, frigid, and subtropical regions, promoting nutrient cycling and carbon fixation in forest ecosystems. The water and nutrients absorbed by the ectomycorrhizal fungi in the soil are transported to the ectomycorrhizal through the hyphae, and then transferred to the host plant t...

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Problems solved by technology

[0004] But the symbiotic culture method of existing chestnut and ectomycorrhizal fungus all has certain defect, mainly is: (1) inoculation method is complicated: the inoculation method that exists at present has two kinds: the first kind of mixed substrate culture method, substrate needs according to Mixing in a certain proportion, the process is cumbersome and the proportion is difficult to control

The second in vitro culture method requires a large amount of culture medium and aseptic operation, which is a heavy workload and difficult to operate

(2) The efficiency of mycorrhizal symbiosis is low and unstable: In the in vitro culture method, usually only a very small number of mycorrhizae are formed or no mycorrhizae are formed in each chestnut seedling, and dozens or even hundreds of chestnut seedlings can provide the experimental results. The number of mycorrhizae is required, the workload is large, and the genetic background of mycorrhizae is chaotic, which is not easy for subsequent experimental analysis

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