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A kind of mycorrhizal multi-inoculation method and application of woody plants

A woody plant and mycorrhizal technology, applied in the field of multi-inoculation of woody plant mycorrhiza, can solve the problem of microbial community restoration, soil improvement and fertility restoration. It cannot better promote plant colonization, and the effect is not as good as multi-inoculation Plants and other problems, to achieve the effect of promoting the diversity of microbial communities, rapid mycorrhizal rate of infection, and not easy to interfere with bacteria

Inactive Publication Date: 2021-05-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the inoculation technology of single ectomycorrhizal fungi is mostly used. For plants inoculated with a single fungus, although the existence of ectomycorrhizal fungi has enhanced the ability of trees to resist stresses such as heavy metals, drought, salt and diseases, but far less effective than inoculating plants
For abandoned and barren lands such as wastelands and mines, single inoculation cannot better promote plant colonization due to the limitation of inoculated fungal species, and has limited effects on the restoration of microbial communities, soil improvement and fertility restoration.
[0004] Foreign countries have used culture medium to culture mycelium for direct double inoculation. This method has a high pollution rate and is easily disturbed by bacteria. In addition, the medium is not an excellent substrate for plant growth, and the growth of plants cultured in the medium is relatively slow.
At the same time, domestic mycorrhizal double-strain inoculation and multi-inoculation techniques are rarely reported

Method used

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  • A kind of mycorrhizal multi-inoculation method and application of woody plants
  • A kind of mycorrhizal multi-inoculation method and application of woody plants
  • A kind of mycorrhizal multi-inoculation method and application of woody plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Pisolithus tinctorius and Cenococcum geophilum inoculation

[0055] S1 Cultivation of plant seedlings to be inoculated: After soaking in 75% alcohol for 30 seconds, soaking in 10% sodium hypochlorite for 10 minutes, and then washing the seeds with sterilized water for 3 times, put the sterilized black pine seeds in a 25°C incubator to accelerate germination. When the seeds start to show white, they are sown into the culture substrate sterilized at 121° C. for 2 hours. The substrate used is vermiculite, and the cultivation environment is a sun room with a temperature of 20-25° C.

[0056] S2: After the seeds described in S1 are cultivated until they grow into seedlings, cut off the root tip of the tap root and keep the 1.5 cm tap root.

[0057] S3: Fix the two plastic flowerpots close to each other, and cut a gap of 1cm in depth on the two adjacent surfaces.

[0058] S4: The seedlings of S2 are placed in the gap, and the lateral roots of the seedlings are ...

Embodiment 2

[0065] Example 2 Laccaria bicolor and Laccaria amethystina inoculation

[0066] S1 Cultivation of plant seedlings to be inoculated: After soaking in 75% alcohol for 30 seconds, soaking in 10% sodium hypochlorite for 10 minutes, and then washing the seeds with sterilized water for 5 times, put the sterilized black pine seeds in a 25°C incubator to accelerate germination. When the seeds start to show white, they are sown into the culture substrate sterilized at 121° C. for 2 hours. The substrate used is vermiculite, and the cultivation environment is a sun room with a temperature of 20-25° C.

[0067] S2: After the seeds described in S1 are cultivated until they grow into seedlings, the root tip of the tap root is cut off, and the 2.5 cm tap root is retained.

[0068] S3: Fix the two seedling pots close to each other, and cut a gap with a depth of 2 cm on the two adjacent surfaces.

[0069] S4: The seedlings of S2 are placed in the gap, and the lateral roots of the seedling...

Embodiment 3

[0076] Example 3 Pisolithus tinctorius , Cenococcum geophilum , Laccaria bicolor , Laccaria amethystina inoculation

[0077] S1 Cultivation of plant seedlings to be inoculated: After soaking in 75% alcohol for 30 seconds, soaking in 10% sodium hypochlorite for 10 minutes, and then washing the seeds with sterilized water for 5 times, put the sterilized black pine seeds in a 25°C incubator to accelerate germination. When the seeds start to show white, they are sown into the culture substrate sterilized at 121° C. for 2 hours. The substrate used is vermiculite, and the cultivation environment is a sun room with a temperature of 20-25° C.

[0078] S2: After the seeds described in S1 are cultivated until they grow into seedlings, the root tip of the tap root is cut off, and the 2.5 cm tap root is retained.

[0079] S3: Fix the 4 seedling pots close to each other, and cut a gap with a depth of 2 cm on the adjacent surfaces of the 4 seedling pots.

[0080] S4: The seedlings ...

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Abstract

The invention provides a multi-mycorrhizal inoculation method for woody plants, which overcomes the limitation of a single strain and achieves the effect that the same woody plant can be infected by multiple ectomycorrhizal fungi, which is better than single strain inoculation Promote plant growth, colonization and improve plant stress resistance. At the same time, this method can be applied to the vegetation restoration of abandoned land, and multi-inoculated seedlings can better promote the diversity of microbial communities in abandoned land, which has a positive effect on the improvement of abandoned land. After the waste land is improved by inoculating more seedlings, it is conducive to the improvement of biodiversity, and has a positive effect on land fertility, soil physical and chemical properties and other indicators. The inoculation of the present invention uses the successfully infected mother seedling mycorrhizae to inoculate the seedlings, and the pollution rate is greatly reduced. At the same time, the soil matrix is ​​used to cultivate the plants to be inoculated, and the plants grow vigorously.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution control and vegetation restoration of abandoned land, and in particular relates to a woody plant mycorrhizal multi-inoculation method and its application. Background technique [0002] Ectomycorrhizal fungi (ECM) are a class of large fungi that can form a symbiotic relationship with the roots of higher plants. Plant-ectomycorrhizal fungus symbiosis is a common phenomenon in nature. The use of ectomycorrhizae can make trees mature 4-5 years in advance. Ectomycorrhizal fungi are conducive to the growth of lush forests in poor soils. In low-nutrient soil environments, ectomycorrhizal fungi are still highly infectious, which is of great significance to the vegetation restoration of wasteland, mines and other abandoned land. Ectomycorrhizal fungi play a vital role in maintaining ecological balance and protecting biodiversity. They are an important part of maintaining the balance of for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G17/00A01G18/10A01G7/06A01G24/15A01G24/10
CPCA01G7/06A01G17/005A01G31/00A01G24/10A01G24/15
Inventor 陈亚华刘镔皞史良王杰蒋明里夏妍沈振国
Owner NANJING AGRICULTURAL UNIVERSITY
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