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Brevibacillusreuszeri and application thereof in promoting pine tree growth

A technology of Bacillus brevis and fungi, applied in the field of microorganisms, can solve problems such as unreported, and achieve the effects of promoting growth, excellent strain resources, and good application and development prospects

Active Publication Date: 2013-07-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening and application of mycorrhizal auxiliary bacteria of Pinus massoniana

Method used

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  • Brevibacillusreuszeri and application thereof in promoting pine tree growth
  • Brevibacillusreuszeri and application thereof in promoting pine tree growth
  • Brevibacillusreuszeri and application thereof in promoting pine tree growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The growth-promoting effect of the mycorrhizal helper bacterium MPt17 on the exophytic mycorrhizal fungus Colored Bean Puffball (Pt2):

[0029] Take the preserved MPt17 strain, in KMB medium, 28°C, after activating for 2-3 days, use an inoculation loop to inoculate a small amount into a 250mL Erlenmeyer flask filled with 50mL KMB liquid medium, culture at 28°C, 180r / min shaking for 72h . Pour the bacterial suspension into a sterilized centrifuge tube, centrifuge at 5000r / min at 4°C for 5min, pour off the supernatant, then wash the bacteria twice with normal saline, and finally add physiological Saline, after mixing it is the bacterial cell suspension (OD 600 =1.5~2).

[0030] The test was performed using the dry dish confrontation (Dunstan et al, 1998) method. Cultivate the colored bean puffball (Pt2) in PDA medium at 25°C for 7-12 days, use a hole puncher with a diameter of 7mm to punch holes on the edge of the petri dish, and take bacterial blocks with a ...

Embodiment 2

[0034] Example 2 Effects of the extracellular metabolites of the mycorrhizal helper bacterium MPt17 on the growth of the mycelium of the exophytic mycorrhizal fungus Colored Bean Puffball (Pt2):

[0035] After activating MPt17 (the method is the same as in Example 1), a small amount was inoculated with an inoculation loop into a 250mL Erlenmeyer flask containing 50mL of KMB liquid medium, and cultured at 28°C with shaking at 180r / min for 72h. Pour the bacterial suspension into a sterilized centrifuge tube, centrifuge at 5000r / min at 4°C for 5min, draw the supernatant with a sterile syringe, and filter it through a bacterial filter (the pore size of the filter membrane is 0.22um) to obtain a sterile filtrate.

[0036]Mix 1 mL of the sterile filtrate with 20 mL of PDA at 45°C and pour it onto a plate, and treat it with sterile water as a control. The colored bean puffball (Pt2) bacterial mass was inoculated in the center of the petri dish, and each treatment was replicated three...

Embodiment 3

[0040] Example 3 The effect of extracellular metabolites of mycorrhizal helper bacteria MPt17 on the biomass of exophytic mycorrhizal fungus Puffball (Pt2):

[0041] The cultured colored bean puffball (Pt2) (PDA medium, 25°C, cultured for 7-12d), was punched with a sterilized hole puncher (d=7mm, ￠=5mm), every 50mL PDA liquid culture Add 3 pieces of bacteria cake to the base, and add 1mL MPt17 extracellular metabolite (the sterile filtrate of Example 2) at the same time, shake the flask at 25°C and 140r / min for 10-12d, filter with qualitative filter paper, and then place at 60°C Baked in an oven to constant weight, and then measured with an electronic balance, with KMB culture medium as the control, each treatment was repeated 3 times. The result is as image 3 As shown, it can be seen that the extracellular metabolites of MPt17 also significantly promoted the biomass of the exomycorrhizal fungus Puffball (Pt2). Compared with the control, the biomass growth rate of Pt2 was 12...

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Abstract

The invention discloses a brevibacillusreuszeri which is categorized and named as brevibacillusreuszeri MPt17. The brevibacillusreuszeri is collected in China Center for Type Culture Collection on December 1st, 2011 with a collection number of CCTCC NO:M2011433. The brevibacillusreuszeri MPt17 is advantaged in that: the brevibacillusreuszeri MPt17 can substantially promote the growth of ectomycorrhiza fungus such as pisolithustinctorius and xerocamuschrysenteron. Also, as a result of greenhouse pot culture experiments, the double-inoculated mycorrhiza auxiliary bacterium MPt17 and pisolithustinctorius or xerocamuschrysenteron can substantially promote the growth and development of pinus massoniana seedlings. Therefore, the brevibacillusreuszeri MPt17 provided by the invention assists in providing excellent strain resources for the development of pinus massoniana growth-promoting microbial manure, and has a good application and development prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a brevibacillus and its application in promoting the growth of Pinus massoniana. Background technique [0002] Ectomycorrhizal Fungi (EMF), as an important group of mycorrhizal fungi, have been proven to have good growth-promoting and disease-resistant effects on plants; at the same time, the application of ectomycorrhizal fungi as microbial fertilizers will not cause environmental pollution , has good environmental friendliness. Plant Growth Promoting Rhizobacteria (PGPR) is a bacterial group in the rhizosphere micro-ecosystem that has a benign effect on plants. PGPR can act on plants alone and show good growth-promoting and disease-resistant effects on plants. The interaction between EMF and ectomycorrhizal fungi has important research value. In recent years, it has increasingly become a hot spot in the field of research on the relationship between rhizosphe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/02A01N63/04A01P21/00C12R1/01
Inventor 叶建仁李倩吴小芹
Owner NANJING FORESTRY UNIV
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