The invention relates to the technical field of polyurethane hot melt adhesives, in particular to a polyurethane hot melt adhesive and a preparation method thereof for seamless integrated vamps. The polyurethane hot melt adhesive comprises, by weight, 60-90 parts of polyester polyol, 20-30 parts of diisocyanate, 2-5 parts of chain extenders and 0.1-2 parts of anti-aging auxiliaries. The polyurethane hot melt adhesive has the advantages that the high-strength bonding characteristic is achieved, materials such as leather and flocking materials can be connected with mesh cloth or other materials in a thermal fusing mode, thereby, seamless integrated shoe making technology can be achieved, bonding between the vamps is quite firm and stable, the appearance is attractive, the melting point of the polyurethane hot melt adhesive is low, the fluidity is good at the hot melting state, the film-forming property is good, cold resistance and bending resistance are good, an adhesive film touches soft, sewing machine staff can be greatly reduced, production processes are reduced, and the production costs are lowered. The preparation method is simple and mature, the production efficiency is high, and general popularization and application can be facilitated.