The invention relates to method for manufacturing antistatic ray-proof worsted fabric, which successively comprises the following steps: firstly, carrying out the cleaning treatment and the sizing and film mulching treatment to rust-less steel fiber, secondly, blending by selecting treated rust-less steel fiber with wool, polyester fiber, bamboo fiber, tenel, modal fiber or spun silk, manufacturing mixed sliver with homogeneous mix, thirdly, spinning the mixed sliver and obtaining the worsted spinning yarn, fourthly, warping, weaving and obtaining the grey fabric, fifthly, carrying out birth repairing, dyeing and finishing and post-processing to the grey fabric, namely obtaining the antistatic ray-proof worsted fabric, wherein the materials feeding proportion by weight of the wool, the polyester fiber, the bamboo fiber, the tenel, the modal fiber, the spun silk, viscose or cashmere are respectively that the rust-less fiber 15-45 parts, the wool 45-75 parts, the polyester fiber 5-50 parts, the bamboo fiber, the tenel, the modal fiber, the spun silk, the viscose or the cashmere 5-20 parts. The method of the invention selectes length and fineness of the rust-less fiber and carries out the prophase film mulching treatment to the fiber, which enables the performances of the fiber is suitable for the requirements of the worsted process and enables the fiber to achieve the goal of being applied at high proportion.