The invention discloses a method for culturing chlorella pyrenoidosa by taking sheep manure leach liquor as a main nitrogen source, and belongs to the field of aquiculture. The chlorella pyrenoidosa is rich in nutrition, widely used for manufacturing healthcare products, cosmetics, medicines, sea fishes and shrimp feeds; a f/2 culture solution and an open cement pit are conventionally adopted to culture, so that the growth is slow, the pollution is easily produced, the cost is high and the benefits are low; at present, a horizontal type white plastic water storage tank is adopted for aerated feeding culture, and the sheep manure leach liquor is taken as the main nitrogen source and added with an appropriate amount of nutritional ingredients such as ammonium nitrate, sodium dihydrogen phosphate, soil extract, DL malic acid, D-sodium erythorbate, potassium silicate, indoleacetic acid, alpha-naphthylacetic acid, medical stone powder, a titanium solution, wheat gluten and hydroxypropyl methyl cellulose, so that not only can the waste be utilized, but also microalgae with a high healthcare value can be obtained; the organic fertilizer and the inorganic fertilizer are mixed for use, so that the nutrition is relatively comprehensive, the operation is convenient, the automatic and large-scale production can be realized, the cost is low, the benefits are high, and the microalgae yield is increased by 240%.