A preparation for rapidly promoting germination and rooting of Tamarix chinensis cuttings and its preparation method

Abstract

The present invention relates to a preparation for rapidly promoting the germination and rooting of Tamarix chinensis cuttings and a preparation method thereof. The raw material formula is: 500-1000 mg / L of indolebutyric acid, 250-500 mg / L of cytokinin, and the rest is double distilled water. Under the treatment of the preparation, the survival rate of Tamarix chinensis cuttings can be rapidly improved, the growth of the aboveground part and the root system can be promoted, and the cutting propagation rate of Tamarix chinensis branches can be significantly improved.

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, in particular to a preparation for rapidly promoting the sprouting and rooting of Tamarix chinensis cuttings and a preparation method thereof. Background Art

[0002] Tamarix chinensis is a common perennial salt-tolerant woody plant in saline-alkali land. It is distributed in both inland and coastal areas of my country. Because of its strong tolerance to salt-alkali stress, it is often planted in large quantities as a pioneer plant for saline-alkali land restoration. During its growth, it can absorb salt ions in the soil and reduce soil salinity. [1] At the same time, it can also absorb and utilize groundwater to achieve the effect of lowering the groundwater level; in addition, through the growth and interlacing of the root system, the pore structure of the soil can be increased, promoting the leaching of salt by precipitation or irrigation. [2] ; It can also promote the formation of soil aggregates through the production of root secretions, improve soil structure and increase soil stability [3] As a pioneer plant for saline-alkali land restoration, Tamarix chinensis plays a huge ecological role in saline-alkali land soil structure restoration and environmental improvement.

[0003] Under natural growth conditions, Tamarix completely relies on seed propagation, but due to the influence of saline-alkali environment, the germination rate of its seeds is extremely low, and the forestation effect is poor. In recent years, many scientific researchers have found in their studies that the method of cutting seedling cultivation can significantly improve the breeding effect of Tamarix and accelerate the improvement of the forestation rate of Tamarix in saline-alkali land. Although compared with seed propagation, cutting technology significantly improves the reproduction rate of Tamarix, its germination rate is still not very high. Therefore, improving the germination rate of Tamarix cuttings and promoting the rooting effect of cuttings have important application value for realizing the restoration of soil structure and improvement of ecological environment of Tamarix in saline-alkali areas.

[0004] At present, there are many brands of rooting powder on the market, the main ingredient of which is naphthaleneacetic acid, which has the effect of improving the vitality of cuttings and promoting their rooting and germination. However, its broad spectrum determines that it is not targeted, especially in the context of large-scale coastal development and coastal shelterbelt construction. How to quickly improve the rooting and germination rate of Tamarix cuttings has important application value for improving the ecological environment of saline-alkali land and shaping the landscape pattern. At present, there is no report on the research of preparations for improving the germination and rooting of Tamarix cuttings.

[0005] Purpose of the invention:

[0006] The purpose of the present invention is to provide a preparation for rapidly promoting the germination and rooting of Tamarix cuttings and a preparation method thereof, which can improve the germination rate of Tamarix cuttings, promote root growth, and enhance the growth vitality of seedlings, thereby obtaining a higher germination rate and seedling survival rate, and realizing the rapid propagation of Tamarix populations using branches.

[0007] The technical solution of the present invention is:

[0008] The germination promoting agent of the invention comprises: gibberellin (GA), indolebutyric acid (IBA), cytokinin (6BA) and naphthaleneacetic acid (NAA), the concentration range of the above preparation is 0-3000 mg / L, 95% ethanol, 0.1 mol / L NaOH solution and double distilled water.

[0009] Preparation method: ① Weigh gibberellins, indolebutyric acid and naphthylacetic acid and dissolve them in a trace amount of 95% ethanol; ② Weigh cytokinin (6BA) and dissolve it in a trace amount of 0.1 mol / L NaOH solution; ③ Dissolve the above four plant hormones in double distilled water and prepare a mother liquor with a concentration of 6000 mg / L; ④ Finally, use double distilled water to make the volume to the required concentration.

[0010] The preparation of the present invention is easy to volatilize, has no residue and no pollution during use, can promote the germination of cutting branches, enhance their rooting and germination ability, and has the advantages of improved quality, high efficiency, low price and the like. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It shows the number of days required for germination and the survival rate of Tamarix chinensis cuttings after being treated with different concentrations of gibberellin (GA), indolebutyric acid (IBA), cytokinin (6BA) and naphthaleneacetic acid (NAA).

[0012] Figure 2 The results show the effects of different concentrations of gibberellin (GA), indolebutyric acid (IBA), cytokinin (6BA) and naphthaleneacetic acid (NAA) on the length of new branches of Tamarix chinensis.

[0013] Figure 3 The paper shows the effects of different concentrations of gibberellin (GA), indolebutyric acid (IBA), cytokinin (6BA) and naphthaleneacetic acid (NAA) on the germination and rooting of Tamarix chinensis cuttings.

[0014] Figure 4 The effect of gibberellin (GA), indolebutyric acid (IBA) and cytokinin (6BA) on the aboveground and underground fresh weight of Tamarix chinensis cuttings, the length of new branches per plant and the survival rate was presented.

[0015] Figure 5 The paper shows the effects of gibberellin (GA), indolebutyric acid (IBA) and cytokinin (6BA) combination on the germination and rooting of Tamarix chinensis cuttings.

[0016] Table 1 shows the effects of different concentrations of gibberellin (GA), indolebutyric acid (IBA), cytokinin (6BA) and naphthaleneacetic acid (NAA) on the fresh weight of aboveground and underground parts of Tamarix chinensis cuttings.

[0017] Figure 1 and Figure 2 The concentrations of gibberellin (GA), indolebutyric acid (IBA), cytokinin (6BA) and naphthylacetic acid (NAA) in the formula are 0, 0-100, 100-250, 250-500, 500-1000, 1000-1500, 1500-2000, 2000-3000 mg / L, respectively.

[0018] First, the survival rate was analyzed and evaluated to screen Figure 1 The relatively excellent plant hormones were selected, and the plant hormone naphthaleneacetic acid (NAA) with a relatively poor survival rate was screened out. Secondly, by analyzing important indicators such as the length of new branches, the fresh weight of above-ground and underground parts, the Figure 2 , Figure 3 and the most excellent concentration ranges of gibberellin (GA), indolebutyric acid (IBA), and cytokinin (6BA) in Table 1, and finally, the combination formed Figure 4 and Figure 5 The formulation, its composition and concentration are as follows:

[0019] Formula A: indolebutyric acid (I) 500-1000 mg / L;

[0020] Formula B: Cytokinin (B) 250-500 mg / L;

[0021] Formula C: Gibberellic acid (G) 0-100 mg / L;

[0022] Formula D: indolebutyric acid (I) 500-1000 mg / L + cytokinin (B) 250-500 mg / L;

[0023] Formula E: indolebutyric acid (I) 500-1000 mg / L + gibberellin (G) 0-100 mg / L;

[0024] Formula F: cytokinin (B) 250-500 mg / L + gibberellin (G) 0-100 mg / L;

[0025] Formula G: indolebutyric acid (I) 500-1000 mg / L + cytokinin (B) 250-500 mg / L + gibberellin (G) 0-100 mg / L;

[0026] By analyzing and evaluating important indicators such as survival rate, new branch length, and fresh weight of above-ground and underground parts, the optimal combination of plant hormones is screened out to form the final formula. DETAILED DESCRIPTION

[0027] Implementation Example 1:

[0028] The raw material formula of the preparation is as follows: the concentrations of gibberellin (GA), indolebutyric acid (IBA), cytokinin (6BA) and naphthylacetic acid (NAA) are 0, 0-100, 100-250, 250-500, 500-1000, 1000-1500, 1500-2000, 2000-3000 mg / L respectively, and the rest is double distilled water.

[0029] Preparation method: ① Weigh gibberellins, indolebutyric acid and naphthylacetic acid and dissolve them in a trace amount of 95% ethanol; ② Weigh cytokinin (6BA) and dissolve it in a trace amount of 0.1 mol / L NaOH solution; ③ Dissolve the above four plant hormones in double distilled water and prepare a mother liquor with a concentration of 6000 mg / L; ④ Finally, use double distilled water to make the volume to be prepared.

[0030] Effect:

[0031] like Figure 1 As shown in the figure, after 30 days of treatment with different plant hormones and their concentrations, there are significant differences in survival rate and germination days. After 21 days of treatment with 0-100 mg / L gibberellin (GA), the survival rate of cuttings reached 100%, after 15 days of treatment with 500-1000 mg / L indolebutyric acid (IBA), the survival rate of cuttings reached 100%, and after 24 days of treatment with 250-500 mg / L cytokinin (6BA), the survival rate of cuttings reached 100%, while the germination rate of cuttings treated with naphthaleneacetic acid (NAA) did not increase significantly. Therefore, in terms of improving the survival rate, the survival rate of cuttings treated with naphthaleneacetic acid (NAA) is significantly worse than the previous three plant hormones, and it is not considered as the preferred plant hormone.

[0032] like Figure 2 As shown in the figure, the number of new branches per plant of Tamarix chinensis treated with 0-100 mg / L gibberellin (GA), 500-1000 mg / L indolebutyric acid (IBA) and 250-500 mg / L cytokinin (6BA) was significantly higher than that of other treatments, which were 1.68, 1.74 and 1.48 times of that of 0 mg / L treatment, respectively. Moreover, the difference between them and 0 mg / L reached a significant level in statistics, indicating that the above concentration range is helpful to promote the growth of new branches.

[0033] As shown in Table 1, the fresh weight of new shoots and roots of Tamarix chinensis treated with 0-100 mg / L gibberellin (GA), 500-1000 mg / L indolebutyric acid (IBA) and 250-500 mg / L cytokinin (6BA) were significantly better than other concentration ranges of the same plant hormone. Compared with the 0 mg / L treatment, the weights increased by 161%, 200%, 114% and 150%, 300%, 150%, respectively, indicating that the above three concentration ranges are helpful to promote the growth of new shoots and roots of Tamarix chinensis, among which 500-1000 mg / L indolebutyric acid (IBA) has the highest improvement effect.

[0034] like Figure 3 As shown, the growth of cuttings treated with 0-100 mg / L gibberellin (GA), 500-1000 mg / L indolebutyric acid (IBA) and 250-500 mg / L cytokinin (6BA) was relatively good, significantly better than other concentration ranges.

[0035] Implementation Example 2:

[0036] The raw material formula of the seed soaking agent is: gibberellin (GA) 0-100 mg / L, indolebutyric acid (IBA) 500-1000 mg / L, cytokinin (6BA) 250-500 mg / L, and double distilled water.

[0037] Preparation method: ① Weigh gibberellin and indolebutyric acid and dissolve them in a small amount of 95% ethanol; ② Weigh cytokinin (6BA) and dissolve it in a small amount of 0.1 mol / L NaOH solution; ③ Dissolve the above three plant hormones in double distilled water and prepare a mother liquor with a concentration of 6000 mg / L; ④ Finally, use double distilled water to make the volume to be prepared.

[0038] Effect:

[0039] Through implementation example 1, after removing naphthaleneacetic acid (NAA), the optimal concentration ranges of three plant hormones were screened out and formulated according to formulas A to G. The implementation effect is as follows: Figure 4 As shown in the figure, the fresh weight of aboveground and underground parts, the length of new branches and the survival rate of the formula D, which was treated with 500-1000 mg / L indolebutyric acid (I) and 250-500 mg / L cytokinin (B), were better than those of the other formulas. Moreover, the growth-promoting effect of the formula D was more obvious than that of the single concentration formula. Figure 5 As shown, the growth condition of formula D is significantly better than that of other formulas, and it is effective in rapidly improving the survival rate of Tamarix cuttings and promoting the growth of above-ground and underground parts.

[0040] The above results show that under the treatment of formula D, the survival rate of Tamarix chinensis cuttings can be quickly improved, the growth of the aboveground part and root system can be promoted, and the cutting propagation rate of Tamarix chinensis branches can be significantly improved.

[0041] References:

[0042] [1] Liu Yahui, Wang Xiuping, Liu Guangming, Sun Jianping, Yao Yutao, Yang Yahua. Accumulation characteristics of salt ions in four typical salt-tolerant plants in coastal saline soil areas [J]. Soils. 2017, 49: 782-788.

[0043] [2]Chen H, Yang C, Ren A, Guo K, Feng X, Li J, Liu

[0044] [3] Li X, Liu

[0045] Table 1.

[0046]

Claims

1. A preparation for rapidly promoting the germination and rooting of Tamarix chinensis cuttings, characterized in that The formula of the raw materials is: 500-1000 mg / L of indolebutyric acid, 250-500 mg / L of cytokinin 6BA, and the rest is double distilled water.

2. The preparation method of the preparation for promoting germination and rooting of Tamarix chinensis cuttings according to claim 1 is characterized in that Proceed as follows: ① Weigh indolebutyric acid and dissolve it in a small amount of 95% ethanol; ② Weigh cytokinin 6BA and dissolve it in a small amount of 0.1 mol / L NaOH solution; ③ Dissolve the above two plant hormones in double distilled water, prepare them immediately before use, and prepare them into a mother solution with a concentration of 6000 mg / L; ④ Finally, use double distilled water to make up to the required preparation volume, and mix the two before use.

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