Cutting slip treatment method for improving survival rate of Chinese wolfberry cutting slips

Abstract

The invention discloses a cutting treatment method for improving the survival rate of lycium barbarum cutting slips, and particularly relates to the technical field of seedling propagation, and the method specifically comprises the following steps: S1, preparing a cutting slip material; s2, setting a hotbed; s3, cutting slips and carrying out root promoting treatment; s4, observing and recording rooting conditions; s5, building a bud eye germination test environment; s6, observing and recording bud eye germination; and S7, carrying out production verification and business application. According to the method, the survival rate of the lycium barbarum cutting slips and the seedling quality are effectively improved, a fresh-keeping container, precise temperature control equipment and the like play a key role in all links, good growth of the cutting slips is promoted, meanwhile, experimental research fills the blank of meteorological indexes of lycium barbarum seedling breeding, a scientific basis is provided for industrial development, and the method has a good application prospect in the production verification and business application stage. By means of an intelligent system, monitoring analysis software and the like, accurate regulation and control of the seedling raising environment are achieved, services are provided for farmers, cost is reduced, sustainable development of the wolfberry industry is promoted, and economic and social benefits are remarkable.

Description

Technical Field

[0001] The invention relates to the technical field of seedling propagation, and in particular to a cutting processing method for improving the survival rate of wolfberry cuttings. Background Art

[0002] As an important economic crop, the quality of wolfberry seedling cultivation is directly related to the development of the wolfberry industry. In the process of wolfberry seedling cultivation, the survival rate of cuttings is one of the key factors. At present, the traditional wolfberry cutting processing method has many limitations, resulting in unstable cutting survival rate and high cost of seedling cultivation.

[0003] Chinese patent announcement number CN108901400A discloses a method for improving the survival rate of black wolfberry hardwood cuttings, the steps of which are: (1) before spring budding, collect one-year-old black wolfberry branches for processing; (2) invert the prepared cuttings in the sand; (3) in mid-to-late March, collect 1-year-old plant source branches, and prepare an extract by soaking; (4) soak the cuttings; (5) cut the treated cuttings; (6) transplant the branches after they take root. This efficient and low-cost black wolfberry hardwood cutting asexual propagation technology can be widely used in black wolfberry hardwood cutting seedling cultivation, with a survival rate of more than 95%, a production cost reduction of more than 30%, and a large number of roots, thick and long branches, and a large growth of branches, which will greatly improve the supply capacity of black wolfberry seedlings and achieve huge economic benefits.

[0004] However, the above method has a low level of sophistication in the equipment and technology for handling cuttings when used, and lacks comprehensive data recording and analysis of the growth process. Summary of the invention

[0005] The main purpose of the present invention is to provide a cutting processing method for improving the survival rate of wolfberry cuttings, which can effectively solve the problems of low refinement of cutting processing equipment and technology and lack of comprehensive growth process data recording and analysis.

[0006] To achieve the above object, the technical solution adopted by the present invention is:

[0007] A method for processing wolfberry cuttings for improving the survival rate of wolfberry cuttings comprises the following steps:

[0008] S1. Preparation of cutting materials: Select annual lignified seed strips of specific varieties that are free of pests and diseases as the source of cuttings;

[0009] S2. Hotbed setting: construct a hotbed for cutting rooting test;

[0010] S3, cutting and rooting treatment: cuttings are cut according to the specifications and placed on the hotbed in a specific way to promote rooting;

[0011] S4. Observation and record of rooting: observe the callus formation and root growth of the cuttings every day, and record the air temperature and soil temperature;

[0012] S5. Construction of the bud germination test environment: set up a small arch shed and equip it with electric heating belts and thermostats to control different temperatures, and divide the areas for different variety tests;

[0013] S6. Observation and record of bud germination: observe the bud germination and growth of the cuttings daily, and record relevant physiological indicators and environmental temperature;

[0014] S7. Production verification and business application: Conduct field batch seedling cultivation verification and apply the research results to the meteorological service of wolfberry seedling breeding.

[0015] Preferably, the step S1 includes a fresh-keeping container, and the fresh-keeping container includes a temperature regulating module, a humidity monitoring module and an ultraviolet disinfection device.

[0016] Preferably, the S2 step includes a heat-insulating matrix composed of dry sheep dung, a plastic film, geothermal wires, bed soil, fine river sand, a temperature controller and a humidity recorder. The temperature controller includes a temperature fine-tuning function with an accuracy of up to +0.5°C and a remote monitoring module. The temperature and humidity recorder includes automatic data storage and wireless transmission functions. The heat-insulating matrix is ​​laid with a thickness of 3-5cm, and the fine river sand is laid with a thickness of 5cm.

[0017] Preferably, the S3 step includes gardening shears, a cutting fixing bracket and an automatic spray device, the blades of the gardening shears are coated with an anti-rust coating, the cutting fixing bracket is made of stainless steel, the bracket height can be adjusted within a range of 10-20 cm, and the spacing adjustment range is 3-5 cm.

[0018] Preferably, the step S4 includes a magnifying glass, a high-precision thermometer and a high-definition camera, and the magnification of the magnifying glass is 5-10 times.

[0019] Preferably, the S5 step includes a plastic film, an electric heating belt, a thermostat, an isolation device for partitioning and an LED plant fill light, wherein the isolation device is made of plastic and has a height of 20 cm.

[0020] Preferably, the step S6 includes a ruler, a recording form and a laser rangefinder.

[0021] Preferably, the S7 step includes an intelligent irrigation system, a precision fertilization device, a soil monitoring sensor, a meteorological data monitoring device, an information publishing platform and data analysis software.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The present invention improves the survival rate of wolfberry cuttings and the quality of seedlings. In the cutting material preparation stage, the fresh-keeping container maintains the activity of the seed strips and reduces the risk of pests and diseases. In the hotbed setting, the temperature controller accurately controls the temperature, the temperature and humidity recorder monitors in real time, the insulation matrix and fine river sand ensure the stability of the hotbed environment, and when the cuttings are cut and rooted, rust-proof gardening shears and reasonable brackets ensure the quality of the cuttings. In the rooting and bud germination stage, a variety of tools assist in accurate observation and recording, timely discover problems and make adjustments. In the whole process, each link cooperates closely, providing the most suitable growth conditions for the cuttings, promoting the rooting of the cuttings and the bud germination, making the root system well-developed and the seedlings strong, and effectively improving the survival rate of the cuttings and the quality of the seedlings.

[0024] 2. The present invention provides a scientific basis and service guarantee for the breeding of wolfberry seedlings. The key growth indicators of wolfberry cuttings of different varieties are studied to fill the gaps in meteorological indicators, provide a basis for selecting the time of cuttings and disaster prevention and mitigation, and in production verification and business applications, intelligent irrigation, precision fertilization and other systems as well as soil and meteorological monitoring equipment realize the precise regulation of the seedling environment. The information release platform and data analysis software can provide real-time information and scientific guidance for growers, reduce the cost of seedling cultivation, improve the efficiency and quality of wolfberry breeding, and promote the sustainable development of the wolfberry industry, with significant economic and social benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The overall process of the present invention Figure 1 ;

[0026] Figure 2 The process of the present invention Figure 2 ;

[0027] Figure 3 The process of the present invention Figure 3 ;

[0028] Figure 4 The process of the present invention Figure 4 ;

[0029] Figure 5 This is a schematic diagram of the experiment of the present invention. DETAILED DESCRIPTION

[0030] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0031] like Figure 1-5 As shown, a method for processing wolfberry cuttings to improve the survival rate of wolfberry cuttings comprises the following steps:

[0032] S1. Preparation of cutting materials: Select annual lignified seed strips of specific varieties that are free of pests and diseases as the source of cuttings;

[0033] S2. Hotbed setting: construct a hotbed for cutting rooting test;

[0034] S3, cutting and rooting treatment: cuttings are cut according to the specifications and placed on the hotbed in a specific way to promote rooting;

[0035] S4. Observation and record of rooting: observe the callus formation and root growth of the cuttings every day, and record the air temperature and soil temperature;

[0036] S5. Construction of the bud germination test environment: set up a small arch shed and equip it with electric heating belts and thermostats to control different temperatures, and divide the areas for different variety tests;

[0037] S6. Observation and record of bud germination: observe the bud germination and growth of the cuttings daily, and record relevant physiological indicators and environmental temperature;

[0038] S7. Production verification and business application: Conduct field batch seedling verification and apply the research results to meteorological services for wolfberry seedling breeding.

[0039] See also Figure 2 The S1 step includes a fresh-keeping container, which can prevent the cuttings from losing water and drying up during storage and transportation, and maintain their cell activity. The fresh-keeping container includes a temperature regulating module, a humidity monitoring module and an ultraviolet disinfection device.

[0040] Implementation process:

[0041] First, put the collected wolfberry seeds into a fresh-keeping container, close the container door to ensure a good seal, turn on the temperature adjustment module of the fresh-keeping container, and set the temperature to a specific value between 2-10°C according to the ambient temperature and the preservation requirements of the seeds. The temperature adjustment module monitors the temperature in the container in real time through a built-in temperature sensor. When the temperature deviates from the set value, the cooling or heating element is started to adjust the temperature to keep the temperature in the container stable.

[0042] The humidity monitoring module works continuously and detects the humidity in the container every 5 minutes. If the humidity is lower than the lower limit of the set suitable humidity range (for example, 60%), the moisturizing device is activated to increase the humidity; if the humidity is higher than the upper limit (for example, 85%), the ventilation device is activated to reduce the humidity to ensure that the humidity is maintained within the appropriate range with an error of no more than ±3%;

[0043] When the seed strips need to be disinfected, place them neatly in the UV disinfection device and adjust the UV intensity between 1000-3000μW / cm2 through the controller according to the number of seed strips and the degree of contamination. 2The appropriate value between the two is set, and the disinfection time is set to a specific duration within 10 to 60 minutes. During the disinfection process, the ultraviolet lamps in the ultraviolet disinfection device are evenly distributed to ensure that all parts of the seed strips can be fully irradiated.

[0044] See also Figure 2 The S2 step includes a heat preservation matrix composed of dry sheep manure, a plastic film, a geothermal line, bed soil, fine river sand, a temperature controller and a humidity recorder. The temperature controller includes a temperature fine-tuning function with an accuracy of up to +0.5°C and a remote monitoring module. The temperature and humidity recorder includes automatic data storage and wireless transmission functions. The heat preservation matrix is ​​laid with a thickness of 3-5cm. Sheep manure has good heat preservation performance and can effectively prevent the heat of the hotbed from dissipating downward and maintain a relatively stable temperature environment in the hotbed. The fine river sand is laid with a thickness of 5cm and is laid on the surface of the bed soil. It has good drainage and air permeability, prevents the bed soil from compacting, and keeps the surface of the hotbed loose. Implementation process:

[0045] At the selected hotbed location, start ground leveling at 8 a.m. Use tools to level the ground to an error of no more than 2 cm, then evenly spread 4 cm thick dry sawdust as a heat preservation matrix between 9 and 10 a.m., compact and level it with tools to ensure uniform thickness;

[0046] A waterproof and heat-insulating plastic film or plastic board is laid on the thermal insulation substrate. The joints between the plastic films or plastic boards are sealed with sealing strips. The sealing work is completed between 11 and 12 o'clock to prevent heat loss and moisture penetration;

[0047] Then, the geothermal wires with automatic temperature control are laid on the plastic film or plastic board according to the predetermined wiring method. The distance between the geothermal wires is kept at 12 cm. The geothermal wires are connected to the temperature controller. The target temperature is set to 15°C through the temperature controller between 1 pm and 2 pm. The temperature controller automatically adjusts the heating power of the geothermal wire with an accuracy of ±0.5°C according to the temperature information fed back by the geothermal wire sensor, so that the temperature of the hotbed is stabilized near the set temperature.

[0048] Cover the top of the laid geothermal line with loose and breathable bed soil with a thickness of 12 cm. Use rakes and other tools to rake the bed soil flat between 2 and 3 pm to ensure that the surface of the bed soil is flat. Evenly lay 5 cm thick fine river sand on the bed soil and use a scraper to scrape the fine river sand flat between 3 and 4 pm. Then install the temperature controller and insert the temperature sensor of the temperature controller 10 cm deep into the bed soil of the hotbed. Complete the installation between 4 and 5 pm to accurately measure the temperature. Set the temperature fine-tuning parameter to ±0.2℃ through the operation panel of the temperature controller. At the same time, configure the relevant parameters for receiving the data transmitted by the temperature controller on the monitoring terminal to ensure that the hotbed temperature data can be received in real time. The configuration work is completed between 5 and 6 pm.

[0049] Install the temperature and humidity recorder, and place the sensors of the temperature and humidity recorder at the four corners and the center of the hotbed. The placement should be completed between 6 and 7 p.m. to comprehensively monitor the temperature and humidity conditions in the hotbed. Set the data storage interval on the temperature and humidity recorder to 1 hour, that is, record the temperature and humidity data once every hour, and configure the wireless transmission parameters so that it can stably transmit the data to the designated storage device or monitoring platform. The configuration work should be completed between 7 and 8 a.m.

[0050] See also Figure 2 The S3 step includes gardening shears, a cutting fixing bracket and an automatic spray device. The gardening shears blade is coated with an anti-rust coating to prevent the shears blade from rusting, maintain the sharpness of the blade, and extend the service life of the gardening shears. The cutting fixing bracket is made of stainless steel, and the bracket height can be adjusted in the range of 10-20 cm, and the spacing adjustment range is 3-5 cm. It can adapt to the needs of different hotbeds and the number of cuttings, ensure that the placement angle and spacing of the cuttings on the hotbed are reasonable, so that the cuttings can fully accept the heat and humidity conditions of the hotbed, which is conducive to the rooting of the cuttings, while avoiding mutual blocking and squeezing between the cuttings, ensuring that each cutting has a good growth space.

[0051] Implementation process:

[0052] From 7:30 to 8:00, check the gardening shears. If the blade is not sharp, use a whetstone to grind it to the point where it can easily cut the one-year-old lignified wolfberry seed strips. From 8:00 to 8:30, disinfect with alcohol wiping method. Use a cotton ball with a concentration of 75% alcohol to wipe the blade, handle and other parts of the gardening shears. If high-temperature disinfection is selected, put the gardening shears into a high-temperature disinfection cabinet from 8:30 to 9:30, set the temperature to 135℃, and disinfect for 12 minutes. After disinfection, store the gardening shears in a clean and dry place for use. The blade has an 8μm thick anti-rust coating to prevent rust.

[0053] From 9:30 to 11:00, use sterilized garden shears to cut the seed strips into 18cm long cuttings, making sure the cuts are flat and smooth;

[0054] From 11 to 12 o'clock, place the cut cuttings on a specially designed stainless steel cutting fixing stand. Adjust the stand height to 15 cm and the cutting spacing to 4 cm so that the cuttings are evenly placed and do not block each other.

[0055] From 12:00 to 13:00, install an automatic spray device for adjusting the humidity of the hotbed, fill the water tank with clean water, and connect the power supply and the nozzle pipe. From 13:00 to 14:00, adjust the spray volume to 80ml / min. The nozzle of the device is an atomizing nozzle, and the diameter of the atomized particles is 80μm, which can spray evenly. The automatic spray device is connected to the temperature and humidity recorder. When the humidity is lower than 60%, the spray will automatically start within 1 minute; when the humidity reaches 80%, the spray will automatically stop within 1 minute, so as to keep the humidity of the hotbed suitable for the growth of cuttings.

[0056] Furthermore, at this stage, it is necessary to clarify the measurement items so that researchers can know clearly what data to collect and how to judge the rooting status when they start to observe the rooting situation, so that the operation process can be naturally connected. The measurement items include:

[0057] During the test, the air temperature and ground temperature in the test box were measured, the rooting rate and germination rate were measured, and the germination index was calculated;

[0058] Rooting: The callus is fully formed and the root system grows out. The rooting rate is calculated as the percentage of roots to the total cuttings.

[0059] Germination: The germination rate is calculated based on the percentage of yellowish buds growing from the cuttings.

[0060] Seedling maturity: The growth of seedlings to 20 cm is taken as the standard, and the seedling rate is calculated as the percentage of mature seedlings to the number of sprouts.

[0061] See also Figure 3 The S4 step includes a magnifying glass, a high-precision thermometer and a high-definition camera. The magnification of the magnifying glass is 5-10 times, which can more clearly observe the formation details of the callus tissue at the cutting site, such as color changes, tissue growth status, etc., to help researchers timely and accurately judge the development of the callus tissue.

[0062] Implementation process:

[0063] At 5 a.m., use an 8x magnifying glass to observe the growth of the cuttings. Place the magnifying glass 4 cm away from the cutting site, carefully check the formation of callus tissue, and judge its development degree based on color and texture. This process lasts until 6 a.m.

[0064] From 6:00 to 6:30, place the temperature sensor of a high-precision thermometer with an accuracy of ±0.1℃ 12cm above the hotbed (away from direct sunlight). From 6:30 to 7:00, insert the soil temperature sensor 8cm deep into the bed soil to accurately measure the temperature of the air and soil. After that, read the data every 2 hours. The reading time should be controlled within 5 minutes after the hour and recorded in a special table.

[0065] From 7 to 8 o'clock, turn on the 1080P resolution high-definition camera and set the shooting interval to 4 hours. Adjust the installation position and angle of the equipment to ensure coverage of the entire hotbed area. The equipment has a remote monitoring function, and the image transmission delay does not exceed 5 seconds, and it is connected to the monitoring terminal. After 9 o'clock, researchers can check the growth status of the cuttings at any time. Once abnormalities such as cuttings withering, diseases and insect pests are found, corresponding measures must be taken within 10 minutes to ensure that the growth process of the cuttings is effectively monitored and timely intervened, providing accurate and comprehensive data support for the research.

[0066] See also Figure 3 The S5 step includes a plastic film, an electric heating belt, a thermostat, an isolation device for zoning and an LED plant fill light. The fill light can provide appropriate light intensity according to weather changes and the growth stage of the cuttings, and timely supplement the light when the light is insufficient to ensure the normal photosynthesis of the cuttings, promote the growth of the cuttings and the germination of buds. The isolation device is made of plastic and has a height of 20 cm, which can prevent different varieties of cuttings from affecting each other, such as the spread of pests and diseases, temperature and humidity interference, etc.

[0067] Implementation process:

[0068] At 8 a.m., a small arch shed was built at the selected test site. A plastic film with a light transmittance of 90% and good thermal insulation performance was selected. It was tightened, flattened and fixed on the arch shed frame between 8 and 10 a.m. to ensure that it fits tightly with the ground.

[0069] From 10:00 to 11:30, lay the electric belt according to the layout of the arch shed, with a spacing of 25cm, and then connect the electric belt to the thermostat. From 11:30 to 13:00, set the temperature for different time periods on the LCD screen of the thermostat, such as 20℃ from 8:00 to 18:00 and 15℃ from 18:00 to 8:00 the next day. The thermostat controls the temperature with an accuracy of ±1℃ based on the feedback information from the electric belt sensor;

[0070] From 13:00 to 15:00, install plastic isolation devices with a height of 20 cm and a joint gap of no more than 1 mm, divide different areas for different varieties of tests, and ensure that the joints are tight to prevent interference during installation;

[0071] From 15:00 to 17:00, LED plant supplementary lights are installed in each area. When it is cloudy or the light is insufficient, the light intensity is increased to 4000 lux. The light is supplemented for 10 hours a day. By adjusting the light emission ratio of the lamp beads to simulate natural sunlight, sufficient and appropriate light is provided for the cuttings, creating an environment conducive to their growth.

[0072] See also Figure 3 The S6 step includes a ruler, a recording form and a laser rangefinder. The ruler accurately records the height changes of the cuttings at different growth stages. The recording form records in detail information such as the bud germination date, the growth height and the number of leaves, and comprehensively records the changes in key indicators during the growth of the cuttings, which is convenient for researchers to organize and analyze the data. The laser rangefinder can provide high-precision growth height measurement when the cuttings grow high or the growth environment is complex and difficult to measure accurately with a ruler, thereby ensuring the accuracy of the measurement data.

[0073] The germination index and germination rate index used in seed germination rate evaluation were introduced to calculate the germination rate of wolfberry seedlings:

[0074] Germination Index The germination index is a deepening of the germination rate index and is the sum of the number of germinations per day. t The day of the germination test, G t The number of germinations on that day.

[0075] Germination rate index GR: Reference to Maqu i re's germination rate (Speed ​​of germination) index: G i At time t i (in days) Germination percentage, G i The index is related to the germination rate and duration of seedlings, that is, its value is higher under conditions of fast germination and short germination period.

[0076] Implementation process:

[0077] Starting from the first day of cuttings, observations and records were made at 9:00 every day. First, a ruler with a minimum scale of 1 mm was placed vertically next to the cuttings, with the zero scale aligned with the base. The corresponding scale value of the bud tip was read from 9:00 to 9:10, accurate to 1mm, and recorded in a table containing items such as bud germination date, growth height, and number of leaves.

[0078] During observation, if buds are found to have sprouted (light yellow buds appear), immediately record the date as the bud sprouting date. From 9:10 to 9:20, carefully count the number of leaves and record them.

[0079] When the cuttings grow high or the environment is complex and it is inconvenient to use a ruler, from 9:20 to 9:30, place a laser rangefinder with a measuring accuracy of ±1 mm in a stable position, aim the laser at the top of the cuttings, read the displayed value and record it as the growth height; from 9:30 to 9:40, organize and save the table to ensure that the data is complete and accurate, providing a reliable basis for analyzing the growth status of the cuttings.

[0080] See also Figure 3 and Figure 4 The S7 step includes an intelligent irrigation system, a precision fertilization device, a soil monitoring sensor, a meteorological data monitoring device, an information release platform and data analysis software. The meteorological data is updated every 15 minutes and can promptly reflect changes in environmental meteorological conditions. The information release platform supports multi-terminal information reception so that growers or researchers can obtain data anytime and anywhere. The data analysis software conducts in-depth analysis of the collected environmental factor data such as temperature, humidity, soil fertility and the growth indicators of wolfberry cuttings, and proposes the key factors affecting the growth of cuttings and their optimal value range, so as to achieve the goal of efficient seedling cultivation and improving the quality of wolfberry seedlings.

[0081] Implementation process:

[0082] When conducting field batch seedling verification, first install an intelligent irrigation system in the seedling field. According to the growth stage requirements of wolfberry cuttings, set the humidity threshold on the system control panel, such as 50% lower limit and 70% upper limit during the rooting period. The soil humidity sensor monitors in real time. When the humidity is lower than the lower limit, the system starts irrigation equipment such as drip irrigation pipes or sprinklers within 1 minute, and calculates the irrigation amount according to the water shortage. When the upper limit is reached, stop irrigation to ensure that the soil humidity is appropriate.

[0083] At the same time, a precision fertilization device is installed to accurately control the amount and time of fertilization according to growth needs. Soil monitoring sensors monitor soil temperature (accuracy ±0.5℃), humidity (±3%), fertility (±5ppm) and other parameters. Meteorological data monitoring equipment updates temperature (±0.5℃), precipitation (±0.1mm), light (±10l ux) and other data every 15 minutes;

[0084] The information release platform supports mobile phone APP and computer web pages to receive real-time or scheduled push monitoring data. The data analysis software mines and statistically analyzes the correlation between environmental factors and cutting growth indicators (with an accuracy of no less than 90%), guides production based on the analysis results, and improves the level of wolfberry seedling breeding.

[0085] See also Figure 4 and Figure 5 The overall experimental process of the root formation stage of cuttings is as follows:

[0086] The rooting test of cuttings was carried out in the production workshop of the base. By setting up hotbeds with different temperatures, the rooting rate, number of roots, and root length of wolfberry cuttings were studied.

[0087] Hotbed setting: Set up 8 2m*2 hotbeds, and lay a 3-5cm layer of insulation substrate such as dry sawdust or dry sheep manure at the bottom of the bed to prevent the heat of the bed soil from transferring downward and dissipating heat to ensure the temperature of the hotbed. Cover the insulation substrate with plastic film or plastic board to block the connection between the bed soil and the insulation material and the bottom soil. Cover the bed soil with plastic film (board), lay geothermal wires in the bed soil, and then lay about 5cm of fine river sand. Install a temperature controller outside the bed, and install a temperature and humidity recorder to record temperature changes. The temperature range of the 8 hotbeds is set at 6-20℃, with a temperature gradient of 2℃.

[0088] Cuttings cutting: according to the technical specifications of wolfberry cuttings seedling cultivation, the one-year-old seed cuttings should be cut.

[0089] Rooting treatment of cuttings: Place 50 cuttings in a bundle on the hotbed, with the slanted end facing upward and the flat end facing downward, and place them upside down. Other operations for rooting are the same as conventional work.

[0090] Observation records: Observe the callus formation at the cutting site every day, record the number of days from callus formation to root formation under different treatments, record the root length, and record the air temperature and soil temperature hour by hour.

[0091] Experimental process of cutting bud germination stage:

[0092] Set up 8 small arch sheds of 2m*2 in size, with a height of 1.2m. The temperature in the arch shed is controlled by electric belt + thermostat, and the temperature control is set to 6, 8, 12, 16, 18, 20, 24, and 26℃ respectively. Cuttings with a root length of about 1cm were selected for transplanting. Each small arch shed was divided into three areas for three different varieties of experiments. Each variety was repeated 3 times (a, b, c), and 20 cuttings were transplanted in each repetition, which means that each shed was planted with: 20 (roots) x 3 (repetitions) x 3 (variety), 180 cuttings.

[0093] The growth and development of the cuttings were observed daily, and the physiological shapes such as the bud germination date and seedling growth height under different treatments were recorded. At the same time, the air temperature and soil temperature were recorded.

[0094] The following are the experimental results:

[0095] Rooting situation: Rooting rate: Under 6-20℃ hotbed treatment, the rooting rate is higher at 14-18℃, averaging 75%; Ningqi No. 1 reaches a maximum of 82% at 16℃, Ningqi No. 7 is 78% at 14℃, and Qixin No. 1 reaches 80% at 18℃.

[0096] Number of roots and root length: Under suitable temperature (14-18℃), the average number of roots per plant is 12, the root length is 15cm, and the roots are strong; under unsuitable temperature, such as 6℃, the average number of roots per plant is 3, and the root length is 5cm.

[0097] Time from callus formation to root generation: an average of 12 days at suitable temperature (14-18℃), and the fastest 10 days at 16℃; an average of 18 days at 20℃, and the longest 25 days at 6℃.

[0098] Bud germination situation: Bud germination rate: In the test at 6-26℃, 16-20℃ was higher, averaging 85%; Ningqi No. 1 reached 90% at 18℃, Ningqi No. 7 reached 88% at 16℃, and Qixin No. 1 reached 86% at 20℃.

[0099] Bud germination time: 10 days on average at suitable temperature (16-20℃), 8 days at the earliest at 18℃, and delayed to 20 days at 6℃.

[0100] Seedling growth height: At suitable temperature (16-20℃), the average daily growth is 1.5cm, and it can reach 30cm in 20 days at 18℃, with strong growth; at 6℃, it is only 10cm in 20 days, with slow growth.

[0101] In the test of rooting and bud germination of wolfberry cuttings, after comparing different temperature treatments, it was found that 16°C was the optimal temperature in the rooting stage. At this temperature, the rooting rate of Ningqi No. 1 was as high as 82%, and the comprehensive performance of root quantity and root length was the best; 18°C ​​performed best in the bud germination stage. At this time, the bud germination rate of Ningqi No. 1 reached 90%, and the seedlings grew rapidly and strongly.

[0102] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A method for treating wolfberry cuttings to improve the survival rate of wolfberry cuttings, comprising the following steps: S1. Preparation of cutting materials: Select annual lignified seed strips without pests and diseases as the source of cuttings; S2. Hotbed setting: construct a hotbed for cutting rooting test; S3, cutting and rooting treatment: cutting the cuttings and placing them on a hotbed to promote rooting; S4. Observation and record of rooting: observe the callus formation and root growth of the cuttings every day, and record the air temperature and soil temperature; S5. Construction of the bud germination test environment: set up a small arch shed and equip it with electric heating belts and thermostats to control different temperatures, and divide the areas for different variety tests; S6. Observation and record of bud germination: observe the bud germination and growth of the cuttings daily, and record relevant physiological indicators and environmental temperature; S7. Production verification and business application: Conduct field batch seedling verification and apply the research results to meteorological services for wolfberry seedling breeding.

2. A method for treating wolfberry cuttings for improving the survival rate of wolfberry cuttings according to claim 1, characterized in that: The step S1 includes a fresh-keeping container, and the fresh-keeping container includes a temperature adjustment module, a humidity monitoring module and an ultraviolet disinfection device.

3. A method for treating wolfberry cuttings for improving the survival rate of wolfberry cuttings according to claim 1, characterized in that: The S2 step includes a heat preservation matrix composed of dry sheep dung, a plastic film, a geothermal line, bed soil, fine river sand, a temperature controller and a humidity recorder. The temperature controller includes a temperature fine-tuning function with an accuracy of up to +0.5°C and a remote monitoring module. The temperature and humidity recorder includes automatic data storage and wireless transmission functions. The heat preservation matrix is ​​laid with a thickness of 3-5cm, and the fine river sand is laid with a thickness of 5cm.

4. A method for treating wolfberry cuttings for improving the survival rate of wolfberry cuttings according to claim 1, characterized in that: The S3 step includes gardening shears, a cutting fixing bracket and an automatic spray device. The blades of the gardening shears are coated with an anti-rust coating. The cutting fixing bracket is made of stainless steel. The bracket height can be adjusted within a range of 10-20 cm, and the spacing can be adjusted within a range of 3-5 cm.

5. A method for treating wolfberry cuttings for improving the survival rate of wolfberry cuttings according to claim 1, characterized in that: The step S4 includes a magnifying glass, a high-precision thermometer and a high-definition camera. The magnification of the magnifying glass is 5-10 times.

6. A method for treating wolfberry cuttings for improving the survival rate of wolfberry cuttings according to claim 1, characterized in that: The S5 step includes a plastic film, an electric heating belt, a thermostat, an isolation device for partitioning and an LED plant fill light. The isolation device is made of plastic and has a height of 20 cm.

7. A method for treating wolfberry cuttings for improving the survival rate of wolfberry cuttings according to claim 1, characterized in that: The step S6 includes a ruler, a recording form and a laser rangefinder.

8. A method for treating wolfberry cuttings for improving the survival rate of wolfberry cuttings according to claim 1, characterized in that: The S7 step includes an intelligent irrigation system, a precision fertilization device, a soil monitoring sensor, a meteorological data monitoring device, an information release platform and data analysis software.

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