Protein post-translational modification positioning method

A post-translational modification and protein technology, applied in the field of proteomics and bioinformatics, can solve problems such as data confusion, protein variant accuracy to be discussed, missing information, etc., to achieve precise positioning improvement, efficient analysis and accurate structure identification Effect

Inactive Publication Date: 2015-08-05
TONGJI UNIV
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Problems solved by technology

However, in experiments, a single mass spectrogram often contains multiple protein variants. This method only outputs one result, and the pursuit of the best matching protein variant loses a lot of information.
Moreover, the accuracy of some of the so-called best-fit protein variants is still up for debate due to the confounding of data for multiple protein variants

Method used

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  • Protein post-translational modification positioning method

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Embodiment

[0033] A method for protein post-translational modification localization, comprising the following steps:

[0034] (1) According to the sequence and modification information in the standard protein sequence database, generate the theoretical primary mass spectrometry database and secondary mass spectrometry database of all corresponding protein variants;

[0035] (2) compare the experimental primary mass spectrum with the theoretical primary mass spectrum, and find all candidate protein variants that match the primary mass spectrum;

[0036](3) Matching the experimental secondary mass spectrometry data with the theoretical secondary mass spectrometry data of all candidate protein variants to obtain the matching fragment ion information of each candidate protein variant;

[0037] (4) Select a candidate protein variant, if it does not contain post-translational modification, then no post-translational modification localization scoring; if it contains post-translational modificat...

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Abstract

The invention relates to a protein post-translational modification positioning method. On the basis of original primary and secondary mass spectrum data base searching, loci of protein post-translational modification are determined by feature matching ions, and high efficiency and accuracy in distinguishing of position isomerism or combination isomerism of post-translational modification of different proteins can be realized by the aid of characteristic ions during protein identification and data base searching of mass spectrum data, so that protein post-translational modification positioning is realized. Compared with the prior art, the method has the advantages that accuracy in high-throughput protein post-translational modification positioning is remarkably improved, comprehensive post-translational modification identification results can be acquired, and protein post-translational modification loci are determined accurately. In addition, the method is applicable to high-throughput proteome analysis and especially applicable to accurate structural identification and efficient analysis of tandem mass spectrums and spectrums with a great quantity of post-translational modification proteins (such as proteomes and the like).

Description

technical field [0001] The invention relates to a protein analysis method, in particular to a protein post-translation modification positioning method, which belongs to the field of proteomics and bioinformatics related to biological mass spectrometry. Background technique [0002] With the development and commercialization of soft ionization techniques (such as electrospray ionization) and high-resolution mass analyzers (such as orbitrap), mass spectrometry is increasingly used in the research and analysis of proteomics. [0003] Chinese patent CN 103389335 A discloses an analysis device and method for identifying the primary structure and composition of biological macromolecules by analyzing the mass spectrum data of biological macromolecules. The analysis device and method are based on the original primary and secondary mass spectra of the biomacromolecules, and identify the biomacromolecules by comparing isotopic peak mass-to-charge ratios and profile fingerprints. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 肖开捷沈赟王悦田志新
Owner TONGJI UNIV
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