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Molecular marker, kit and method for distinguishing Heilongjiang megalobrama terminalis and megalobrama amblycephala and hybrid of Heilongjiang megalobrama terminalis and megalobrama amblycephala

A technology of molecular markers and hybrids, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of inability to accurately identify hybrids, unclear natural hybrids, etc., and achieve accuracy High, short time, simple operation process effect

Active Publication Date: 2020-10-30
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the existing technology is unable to accurately identify Heilongjiang triangular bream, grouphead bream and their hybrids. Therefore, it is still unclear whether there are natural hybrids of the two in the relevant sections of the Heilongjiang River Basin. Technological approaches for bream and its hybrids to better protect the germplasm resources of endangered Heilongjiang triangular bream

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  • Molecular marker, kit and method for distinguishing Heilongjiang megalobrama terminalis and megalobrama amblycephala and hybrid of Heilongjiang megalobrama terminalis and megalobrama amblycephala
  • Molecular marker, kit and method for distinguishing Heilongjiang megalobrama terminalis and megalobrama amblycephala and hybrid of Heilongjiang megalobrama terminalis and megalobrama amblycephala
  • Molecular marker, kit and method for distinguishing Heilongjiang megalobrama terminalis and megalobrama amblycephala and hybrid of Heilongjiang megalobrama terminalis and megalobrama amblycephala

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specific Embodiment approach 1

[0023] Specific embodiment one: The present invention distinguishes the primer pair of the molecular markers of Heilongjiang triangular bream and group head bream and their hybrids as HLJFLf2 and HLJFLr2, and the specific sequences are:

[0024] HLJFLf2: 5'-GCAACCGTTTGTCTGCTATG-3'

[0025] HLJFLr2: 5'-CCACAGCCTTAGATGGAATC-3'.

specific Embodiment approach 2

[0026] Specific embodiment two: the kit for distinguishing Heilongjiang triangular bream from bream and their hybrids, which is characterized in that the kit includes a pair of molecular marker primers HLJFLf2 and HLJFLr2.

specific Embodiment approach 3

[0027] Specific embodiment three: the difference between this embodiment and specific embodiment two is that the kit also includes deionized water, 10×Taq Buffer, MgCl 2 , dNTPs, Taq DNA polymerase, RE buffer 10×Buffer, bovine serum albumin BSA, TaqI endonuclease and molecular marker primer pair HLJFLf1 and HLJFLr1; where, HLJFLf1: 5'-GCATCTGGCTTCAATCTCA-3'; HLJFLr1: 5' -GATTTGCTGAGCGTAGGG-3'. Others are the same as in the second embodiment.

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Abstract

The invention discloses a molecular marker, kit and method for distinguishing Heilongjiang megalobrama terminalis and megalobrama amblycephala and hybrid of Heilongjiang megalobrama terminalis and megalobrama amblycephala, and relates to a molecular marker and a kit for distinguishing Heilongjiang megalobrama terminalis and megalobrama amblycephala and their hybrid and an application thereof. According to the invention, primer pairs for distinguishing comprise an HLJFLf2 primer pair and an HLJFLr2 primer pair. The kit disclosed by the invention comprises the molecular marker primer pair HLJFLf2 and the molecular marker primer pair HLJFLr2. The method comprises the following steps: 1, extracting genome DNA to be detected, 2, carrying out PCR amplification by taking the extracted genome DNAas a template and taking HLJFLf2 and HLJFLr2 as primers to obtain a PCR product, and 3, carrying out electrophoresis detection on the PCR product, wherein the individual corresponding to the electrophoresis result that only the 249bp band appears is the pure Heilongjiang megalobrama terminalis, the individual only the 335bp band appears is the pure megalobrama amblycephala, and the individual withthe 249bp band and the 335bp band appears is the hybrid of the Heilongjiang megalobrama terminalis and megalobrama amblycephala.

Description

technical field [0001] The invention relates to a molecular marker for distinguishing Heilongjiang triangular bream from group-headed bream and hybrids thereof, a kit and an application thereof. Background technique [0002] There are 4 existing species of bream in my country, which are pachygnathic bream in the upper reaches of the Yangtze River, grouphead bream (Wuchang fish) in large lakes in the middle and lower reaches of the Yangtze River, Guangdong bream in various water systems of the Pearl River and Hainan, and bream widely distributed in multiple water systems. Triangle bream. [0003] Heilongjiang triangular bream is the "Faro" in the famous fish "San Hua Wu Luo" unique to the Heilongjiang River Basin, and it is also the only naturally distributed bream fish in the north of the Yellow River. The fish is large in size, strong in stress resistance, high in fat and delicious in taste, and has great breeding potential; on the other hand, the long-term geographical iso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888
Inventor 胡雪松石连玉栾培贤姜晓娜贾智英李池陶葛彦龙王世会张秋军
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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