Molecular marker BanII-1 related to muskmelon powdery mildew resistance and application thereof

A powdery mildew and molecular marker technology for melons, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of few powdery mildew resistant materials of melons and unclear distinction of physiological races of powdery mildew, etc. The effect of speeding up breeding work efficiency, improving breeding efficiency and easy operation

Active Publication Date: 2019-07-26
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are the following technical problems: First, there are few powdery mildew-resistant melon materials, and the physiological races of powdery mildew are not clearly distinguished
Secondly, most of the molecular markers that have been published so far are difficult to closely combine with related traits, so there are very few molecular markers that can be used for gene mapping and molecular marker-assisted selection of powdery mildew resistance in melon

Method used

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  • Molecular marker BanII-1 related to muskmelon powdery mildew resistance and application thereof
  • Molecular marker BanII-1 related to muskmelon powdery mildew resistance and application thereof
  • Molecular marker BanII-1 related to muskmelon powdery mildew resistance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Screening, primer design and application (verification) of molecular marker BanII-1:

[0030] The F 1 , F 2 The generation population is the experimental material verification marker to identify whether the melon is resistant to powdery mildew.

[0031] Two muskmelon lines M4-125 and X055 and F 2 The high powdery mildew resistant plants and the highly susceptible powdery mildew plants in the population were sequenced, and the genome sequence of the melon was obtained through the NCBI website. Through the comparison of the sequence and the sequencing material, the chromosome of the sequence of the segment of the melon powdery mildew resistance gene was obtained. Interval, within this interval, mine the base segment of the sequencing data with the SNP mutation site, analyze the enzyme digestion information of the site, obtain the sequence with the CAPS mutation, and use the Primer software to design the molecular marker BanII on the sequence with the site -1 ...

Embodiment 2

[0049] Example 2 Expanded range application (verification) of molecular marker BanII-1:

[0050] Using the obtained molecular marker BanII-1 to screen the germplasm resources of melon varieties with different resistance to powdery mildew (physiological race 2F of Erysipha monocysticum):

[0051] In order to identify the applicability of the developed molecular marker BanII-1 to other melon materials, 10 kinds of melon materials with different resistance to powdery mildew (Erysipha monocysticum race 2F) including M4-125 and X055 were selected. Using the molecular marker primers, the genome was amplified by PCR and verified by enzyme digestion. The names and resistances of these 10 kinds of melon materials are shown in Table 2

[0052] Table 2 Introduction of 10 kinds of melon materials

[0053]

[0054] Expanded range of applications of the molecular marker BanII-1:

[0055] (1) Collect the young true leaves of the above 10 kinds of melon materials, and use the improved C...

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Abstract

The invention discloses a molecular marker BanII-1 related to muskmelon powdery mildew resistance and application thereof and relates to biomolecular markers and application thereof. The application includes following steps: (1), extracting a to-be-detected muskmelon genome; (2), by taking the genome as a template, utilizing molecular marker primer for PCR (polymerase chain reaction) amplificationto obtain a PCR product; (3), performing enzyme digestion on the PCR product to obtain an enzyme digestion product; (4), utilizing agarose gel electrophoresis to identify the enzyme digestion product, and judging a to-be-detected muskmelon plant as a muskmelon single-capsule-shell powdery mildew resistant 2F physiological race or a muskmelon single-capsule-shell powdery mildew nonresistant 2F physiological race. The molecular marker has universality in different materials, and the application is simple and convenient in operation and clear in result. Through a molecular marker assisted selection method, assisted selection of muskmelon powdery mildew resistance at the seedling stage of muskmelon can be realized, and breeding efficiency is improved greatly while breeding cycle is shortened.

Description

technical field [0001] The invention relates to a molecular marker and its application, in particular to a molecular marker related to the powdery mildew resistance of melon and its application. Background technique [0002] Molecular marker-assisted selection (MAS) is a fast and simple method for screening target traits. It can be done in the off-season without environmental constraints, allowing simultaneous screening for several traits. In MAS, molecular markers that are tightly linked to traits of interest are localized in plants. The gene segment of interest is then introduced into the donor plant by crossing, and during backcrossing, molecular markers are used to anchor the gene of interest until the gene of interest is stably inherited in the final plant variety. [0003] Powdery mildew is one of the main fungal diseases affecting the yield and quality of muskmelon. It occurs widely in open field cultivation and protected cultivation of muskmelon. The powdery mildew...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 高鹏崔浩楠栾非时刘识丁卓王学征
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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