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Oryza sativa L. grain shape gene GL3 functional marker and application thereof

A molecular marker, GL3-FM technology, applied in the field of agricultural biology, can solve problems such as undeveloped molecular markers

Pending Publication Date: 2019-07-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

GL3 is a gene that has been cloned and controls rice grain length. There are two base mutations in the GL3 coding region sequence from WY3, one is a 1092bp C-A mutation, and the other is a 1495bp C-T mutation; the mutated GL3 can Significantly increased rice grain length (Qi et al, 2012), but no applicable molecular markers have been developed for the variant loci

Method used

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  • Oryza sativa L. grain shape gene GL3 functional marker and application thereof
  • Oryza sativa L. grain shape gene GL3 functional marker and application thereof
  • Oryza sativa L. grain shape gene GL3 functional marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1. Using Caps-labeled GL3-FM to assist selection to screen single-segment substitution materials carrying GL3-2 under the background of Huajing indica 74.

[0074] Specifically, using the rice variety Huajing Indica 74 as the recipient parent and Star bonnet 99 as the donor parent, a series of rice single-segment substitution line materials were constructed through conventional hybridization and backcrossing. With Huajaponica indica 74 as the female parent and Star bonnet 99 as the male parent, conventional crosses were carried out to obtain F 1 Substitute hybrids, which yield F after selfing homozygous 2 Substitute material, and then on the obtained F 2 Substitute material randomly selected a single plant to use Huajing indica 74 backcross, after at least 3 consecutive backcrosses, self-homozygous. Molecular marker-assisted selection was used for the obtained homozygous materials to determine the chromosome fragments from Star bonnet 99 carried in different i...

Embodiment 2

[0091] Example 2. Identification of rice germplasm resources carrying GL3-2 using molecular marker GL3-FM

[0092] The specific method is: randomly select rice germplasm resources from many countries and regions in the world, extract leaf genomic DNA, and use GL3-FM to identify the allele types of these genetic materials.

[0093] 1. DNA extraction

[0094] Same as Example 1.

[0095] 2. PCR amplification

[0096] Same as Example 1.

[0097] 3. Restriction system of PCR amplification products

[0098] Same as Example 1.

[0099] 4. Detection by agarose gel electrophoresis

[0100] Same as Example 1.

[0101] according to figure 2 , the following conclusions can be drawn: GL3-FM can be used to identify GL3-FM allele types in different rice materials. as figure 2 As shown in the electrophoresis results, the material with only one band carries the GL3-1 allele, and the band pattern is read as "1"; the material with two bands carries the GL3-2 allele, and the band patte...

Embodiment 3

[0107] Example 3. Using molecular marker GL3-FM to carry out assisted selection of granule gene and construction of aggregation line

[0108] Using the constructed, Huajing Indica 74 background, the GW5 allele comes from a single-segment material (named NIL-GW5) of the large-grain rice variety Su Yunuo, which uses the large-grain rice variety Su Yunuo as the donor parent. It was crossed with Huajing Indica 74, and a single plant was selected for continuous backcrossing with Huajing Indica 74 for at least 4 generations in the progeny, and finally it was homozygous for self-crossing. The obtained materials were screened using GW5-specific markers to obtain NIL-GW5. Compared with Huajing Indica 74, the grain shape of NIL-GW5 decreased in rice grain length, but increased significantly in grain width.

[0109] Citation by GW5: Shomura A, et al. Deletion in a gene associated with grainsize increased yields during rice domestication. Nat Genet, 2008, 40: 1023-1028 (Shomura A, et al....

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Abstract

The invention discloses an oryza sativa L. grain shape gene GL3 functional marker (GL3-FM). The oryza sativa L. grain shape gene GL3 functional marker can be applied to identification of oryza sativaL. grain shape improvement and / or of assisted selection of filial generations; single rice plants with amplified fragments incapable of being incised by a restriction enzyme of AflIII carry short-grain-shape GL3-1 alleles; plants with the amplified fragments incised by the AflIII and forming two bands contains GL3-2 alleles capable of inducing grain length increase. The oryza sativa L. grain shapegene GL3 functional marker can be applied to rapid screening of long-grain rice seeds and identification of GL3 alleles to improve the selection efficiency of rice varieties.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology and provides a molecular marker GL3-FM of a rice grain shape gene GL3 and an application thereof. Background technique [0002] Rice (Oryza sativa L.) is a very important food crop. More than half of the population in my country is the staple food of rice, and the consumption population is still expanding (Zuo and Li, 2014; Yuan Longping, 2010). In the past quite a long time, in order to solve the problem of food shortage in our country, the main goal of breeding practice has been to increase the yield. This high-yield-oriented breeding has resulted in poor rice quality of some rice varieties widely promoted in my country. In recent years, people's living standards have been greatly improved, and consumers' requirements for the appearance quality and cooking and eating quality of rice are also increasing (Mo Huidong, 1993; Zhou Kaida et al., 1994; Fitzgerald et al, 2009); With the openi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王少奎张桂权刘新疆朱海涛刘桂富
Owner SOUTH CHINA AGRI UNIV
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