GC-MS detection method of contents of 12 amino acids

A GC-MS and detection method technology, applied in the field of amino acid detection, can solve the problems of capillary electrophoresis, such as low resolution and selectivity, many influencing factors, and chemical methods that cannot be qualitatively analyzed, and achieve rapid and effective qualitative and quantitative analysis and analysis steps Concise, comprehensive effect of measurement types

Inactive Publication Date: 2020-04-28
天津量信检验认证技术有限公司 +2
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  • Application Information

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Problems solved by technology

Among the separation and determination methods of amino acids, the chemical method cannot be qualitatively analyzed, the liquid chromatography method is complicated to operate and has many influencing factors, the resolution and selectivity of the capillary electrophoresis method are low, the automatic amino acid analyzer is expensive, and the operating cost is high, all of which cannot be better. Qualitative and quantitative analysis of various amino acids
[0004] Through the search of the published patent documents, no published patent documents on the GC-MS detection method for the content of 12 amino acids have been found, and no published patent documents identical to this patent application have been found.

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  • GC-MS detection method of contents of 12 amino acids
  • GC-MS detection method of contents of 12 amino acids
  • GC-MS detection method of contents of 12 amino acids

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Embodiment Construction

[0037] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0038] GC-MS detection method for the content of 12 kinds of amino acids. The innovation of the detection method is that the method includes the following steps:

[0039] 1) Preparation of standard working solution:

[0040] Accurately weigh 0.1g of amino acid standard substance, dissolve and dilute it with first-grade water, transfer it to a 100mL volumetric flask, and prepare a solution with a concentration of 1mg / mL, accurately measure 1.0mL for derivatization, pass through a membrane, and wait for sample analysis ;

[0041] 2) Preparation of sample working solution:

[0042] Accurately weigh 8 mL of soy sauce sample, add 3 g of sodium chloride, and place the sample bottle in a 60°C water bath to equilibrate for 10 minutes, then conduct...

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Abstract

The invention relates to a GC-MS detection method of contents of 12 amino acids. The GC-MS detection method comprises the following steps: (1) preparing a standard working solution; (2) preparing a sample working solution; and (3) detecting and analyzing by a GC-MS method. The method disclosed by the invention is scientific and reasonable in design and can be used for carrying out accurate qualitative and quantitative analysis on 12 amino acids so that component detection and analysis are faster, time is saved, detection sensitivity is high and detection data is accurate.

Description

technical field [0001] The invention belongs to the field of amino acid detection and relates to a GC-MS detection method for the content of 12 kinds of amino acids. Background technique [0002] Amino acid is the basic unit of biological functional macromolecule-protein, and an important source of protein synthesis in animals. It plays an important role in various fields of pharmaceutical industry and industrial production. Therefore, amino acid analysis technology is of great significance to protein chemistry, biochemistry and the whole life science research as well as product development, quality control and production management. [0003] Currently, methods for the separation and determination of amino acids are being developed and improved, including chemical methods, capillary electrophoresis, and liquid chromatography. Among the separation and determination methods of amino acids, the chemical method cannot be qualitatively analyzed, the liquid chromatography method ...

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 陈雅琳刘然师静雅徐曼菲周杨李蕊尹亮
Owner 天津量信检验认证技术有限公司
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