48results about How to "Accurate Qualitative and Quantitative Analysis" patented technology

The invention provides a method for detecting 7 classes of 59 forbidden drugs in goat milk and goat milk products. The method comprises the following steps: (1) sample pretreatment: extracting a to-be-detected sample with an acid organic solvent, carrying out purification by virtue of an SPE column, dissolving a purified liquid by virtue of a primary flow phase, and carrying out UPLC-MS/MS detection; and (2) detection of 59 to-be-detected forbidden drugs: detecting the pretreated sample by virtue of an ultra-high performance liquid chromatography-mass spectrometry under specified instrument conditions of the method. The method has the advantages that many drugs can be detected, the type of the drugs is rich, the detection time is short, the sensitivity is high, and a result is accurate andstable; and meanwhile, the detection blank that multiple types and quantity of drugs in the goat milk and the goat milk products can be simultaneously detected is filled up, and the method has important significances to the guarantee of the body health of consumers and the increase of export trade competitiveness of the goat milk and the goat milk products.

The invention discloses a forensic medicine diatom test method.The forensic medicine diatom test method comprises the following steps that sulfamethazine, acrylic acid, acrylamide, N'N-methylene bisacrylamide, ammonium persulfate and deionized water are taken according to a proportion and fully mixed in a beaker; after oxygen is removed with nitrogen, the mixture is poured into a sandwich space formed by two glass slides and then put into an oven at the temperature of 60 DEG C for a polymerization reaction for 3 h, and film-state imprinting hydrogel is prepared; the obtained imprinting hydrogel is soaked in a 10% acetic acid solution, template molecules sulfadimidine in the imprinting hydrogel are removed through ultrasonic oscillation, then the imprinting hydrogel is soaked in a sample to be tested for 5-10 min and then taken out, and then the imprinting hydrogel is put under an optical microscope for observation; diatom on the filter film or a shot picture is checked, classified and counted in a manual recognition mode or an automatic computer recognition mode.The forensic medicine diatom test method is high in yield, efficiency and performance cost ratio, easy to popularize, accurate in qualitative and quantitative analysis and low in labor intensity.

The invention relates to a method for simultaneously detecting multiple phosphorus-based flame retardants in plastics. The method comprises the following steps: (a) establishing a standard curve; (b) pretreating a sample; and (c) detecting the sample. The detection method has the beneficial effects that a selected chromatographic column can realize a good separating effect on the multiple phosphorus-based flame retardants; in addition, fast determination can be carried out on the multiple phosphorus-based flame retardants, and accurate qualitative and quantitative analysis are realized by the method; and the method is good in reproducibility, high in recovery rate, good in precision and simple in operation.

The invention discloses a surface enhanced Raman spectroscopy method for organo-chlorine pesticide residues of fruits and vegetables and belongs to the technical field of detection methods of pesticide residues. The method comprises the following steps: 1, preparing a copper foil substrate; 2, depositing silver nanoparticles on the surface of the copper foil; 3, modifying mercapto on the substrate; 4, performing surface enhanced Raman spectroscopy on organo-chlorine pesticide standard products; and 5, detecting the pesticide residues of the fruits and vegetables. According to the method disclosed by the invention, the sample pretreatment is simple, the detection speed is high, the detection cycle is shortened, the cost is low, and rapid detection of micro/trace pesticides of the fruits andvegetables is realized.

The invention provides a method for detecting 8 categories of restricted drugs in goat milk and products thereof, wherein the number of the restricted drugs is 74. The method comprises: (1) pre-treating a sample: extracting a sample to be detected with an acid organic solvent, purifying with a SPE column, and dissolving the purified liquid by using an initial mobile phase so as to be used for detection with UPLC-MS/MS; and (2) determining 74 restricted drugs: determining the pre-treated sample by using ultra performance liquid chromatography-LC-MS under the instrument condition specified by the method. According to the present invention, the method has advantages of more types of detected drugs, rich variety, short detection time, high sensitivity and accurate and stable results, can makesup for the absence of the simultaneous detection of multiple drugs in goat milk and dairy products, and provides the important significance for the assurance of the health body of consumers and the improvement of the export competitiveness of goat milk and dairy products.

The invention discloses a quick detecting method for sudan red in food, and relates to the field of food safety. The detecting method comprises the four steps of sample pretreatment, concentration and evaporating to dryness, film chromatography, and HPLC determination. The quick detecting method solves the problems that detecting instrument is expensive, complex to operate and not easy to popularize, has the characteristics of high extraction rate, high purifying purity, high recovery ratio and the like, and can achieve the purpose of being quick and reliable, simple and convenient to operate, and cost-saving.

The invention discloses a qualitative and quantitative analysis method for an old negative plate of a lead acid battery. The method comprises the following steps: firstly, dissecting a tested battery, taking out the negative plate, and cleaning the surface separator of the negative plate; washing the negative plate with deionized water until the surface thereof becomes neutral; further soaking the negative plate in boric acid- salicylic acid impregnation liquid for more than 20 minutes while heating, and keeping temperature of the boric acid-salicylic acid impregnation liquid to be 60-80 DEG C during the soaking process; then, taking out and baking the negative plate to completely remove water on the surface thereof; and finally, taking down a lead paste from the dried negative plate, grinding the lead paste into powder, and carrying out qualitative and quantitative analysis on constituents of the negative plate through an XRD(X-Ray Diffraction)-Rietveld method. According to the invention, firstly, the old negative plate drawn out from the battery is pretreated, a protective layer is formed on the surface of the negative plate, oxidization of lead of the negative plate is avoided effectively and residual of sulfuric acid on the surface of the negative plate is removed, as a result, the negative plate can be dried directly by a normal drying method, and the qualitative and quantitative analysis method is high in testing accuracy, convenient and simple, low-cost and pollution-free.

The invention provides a method for detecting the content of monascus pigments in meat products, which comprises the steps of obtaining a proper amount of samples of the meat products to be tested, rubbing and enabling the samples to be uniform; weighing out the prepared samples; extracting with methanol + ethyl acetate solution, dehydrating by adding anhydrous sodium sulfate, and adding ascorbic acid to prevent monascus pigments in the samples from being oxidized; blowing the extract to be nearly dry, diluting the extract to volume with ethyl acetate + cyclohexane solution, and placing the extract for purification treatment; pouring the solution to be purified into a gel permeation chromatography column via a sampling ring; setting high performance liquid chromatography conditions; using the high performance liquid chromatography to determine the solution to be purified; and processing and analyzing data. The method is simple and convenient, rapid, sensitive, accurate and economic and is capable of detecting the monascus pigments in the meat products through qualitative and quantitative analyses of all components of the monascus pigments.

The invention discloses a rapid detection method of sudan red in food, and belongs to the field of food safety. The rapid detection method of sudan red in food comprises four steps including sample pre-treatment, concentration distillation drying, thin layer chromatography, and HPLC measuring. The rapid detection method is capable of solving problems in the prior art that detection instrument is expensive; operation is complex; and popularization is difficult. The advantages are that: the extraction rate is high; the purification purity is high; the recovery rate is high; the rapid detection is rapid and reliable; operation is convenient; and cost is reduced.

The invention relates to a rapid analysis method for a light hydrocarbon component in soluble organic matter of hydrocarbon source rock. The rapid analysis method comprises the following steps that (1) a grinded hydrocarbon source rock sample is accurately weighed and placed into a pyrolysis cup; (2) a C24D50 standard sample and a phenanthrene-d10 standard sample with known concentrations are sucked by a syringe and then added into the pyrolysis cup; (3) the pyrolysis cup is placed in a fume hood, and after standard sample solvents are completely volatilized, the pyrolysis cup is placed into apyrolysis furnace to prepare pyrolysis; (4) a multifunctional pyrolysis instrument is directly connected to a gas chromatography triple-quadrupole mass spectrometry; (5) a liquid nitrogen cold well device is started; (6) a temperature programming condition of the pyrolysis furnace is set; (7) pyrolysis begins, pyrolysis gas enters capillary column with the temperature controlled by a cold well and is enriched, and after a set pyrolysis program is finished, a GC/MS/MS analysis program is automatically started for analysis; (8) the component of a compound is determined by combining characteristic ions and relative retention time of products and searching in an NIST 11 spectral library. The method can be used for realizing rapid and accurate one-time qualitative and quantitative analysis ofsoluble organic components of the hydrocarbon source rock.

The invention provides a test device for a chemical evaporation kinetics behavior, which is used for simulating a pressurizing test of a containment of a nuclear power station, and comprises a simulated pressurizing system, a chemical evaporation system and an analysis system. Wherein the simulated pressurizing system is used to simulating the pressure condition of a pressurizing test of the containment of the nuclear power station, and providing gas with a predetermined pressure to the chemical evaporation system; the chemical evaporation system is used to simulating the evaporation conditions of chemicals in the containment under different gas pressures and flow rates; and the analysis system is used to determine the type of gas volatilized by the chemicals under different gas pressuresand flow rates and related parameters. The test device reproduces the on-site environment by using the simulated pressurizing system and the chemical evaporation system, and simulates the chemical evaporation environment under the pressurizing condition in the containment, the obtained result is true and reliable, and the device can accurately and quantitatively analyze various complex compounds.

The invention relates to a GC-MS detection method of contents of 12 amino acids. The GC-MS detection method comprises the following steps: (1) preparing a standard working solution; (2) preparing a sample working solution; and (3) detecting and analyzing by a GC-MS method. The method disclosed by the invention is scientific and reasonable in design and can be used for carrying out accurate qualitative and quantitative analysis on 12 amino acids so that component detection and analysis are faster, time is saved, detection sensitivity is high and detection data is accurate.

The invention discloses a method for detecting a formaldehyde carbonylation reaction product by high performance liquid chromatography. The method comprises the following steps: (1) preparing a liquid chromatography mobile phase; (2) preparing a mixed standard stock solution, namely respectively weighing glycollic acid, ethylene glycol, a glyoxylic acid aqueous solution, acetic acid, acetaldehyde and a formaldehyde aqueous solution, putting into a volumetric flask, fixing the volume by using an organic solvent, and uniformly shaking to obtain the mixed standard stock solution; (3) preparation of standard solutions: respectively taking 5 parts of the mixed standard stock solution in the step (2), putting the mixed standard stock solution into the volumetric flask, fixing the volume by using an organic solvent, and uniformly shaking to obtain 5 series of standard solutions with different concentrations; (4) establishment of chromatographic analysis conditions; (5) making a standard curve: performing liquid chromatographic analysis, and fitting by taking the concentration as an abscissa and the peak area as an ordinate to obtain the standard curve; and (6) analyzing a reaction product. The method provided by the invention is simple and convenient to operate, good in repeatability, small in analysis error, high in precision and good in sensitivity.