Extraction method of endocrine disruptors

A technology of endocrine disruptors and extraction methods, applied in the preparation of test samples, etc., can solve the problems of poor extraction effect, low extraction efficiency, poor impurity removal function, etc., to achieve the removal of impurities, high extraction efficiency, accurate qualitative and quantitative The effect of the analysis

Inactive Publication Date: 2018-06-15
FOOD INSPECTION CENT OF CIQ SHENZHEN +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, and provide a method for extracting endocrine disruptors, aiming to solve the problems of low extraction efficiency, poor impurity removal function and poor extraction effect in the existing methods for extracting endocrine disruptors. technical problem

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  • Extraction method of endocrine disruptors

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Embodiment 1

[0032] A method for extracting endocrine disruptors, wherein the sample to be tested is subjected to subcritical water extraction and then DLLME processing; details are as follows:

[0033] The subcritical water extraction treatment process is: take 5g solid sample and 2g diatomaceous earth, mix them uniformly and add them into a 10ml extraction pool for extraction treatment; wherein, the extraction solvent is water with 20% acetone as a modifier, and the pH of the extraction solvent is 12. The extraction temperature is 100° C., and the extraction time is 10 min.

[0034] The DLLME treatment process is as follows: add the extract obtained by subcritical water extraction into a conical centrifuge tube, and then add 150 microliters of chlorobenzene as an extraction solvent. The centrifuge tube was vortexed at 2500 rpm for 30 seconds, then centrifuged at 7000 rpm for 5 min, and the chlorobenzene at the tip of the centrifuge tube was recovered with a micro-sampling needle.

Embodiment 2

[0036] A method for extracting endocrine disruptors, wherein the sample to be tested is subjected to subcritical water extraction and then DLLME processing; details are as follows:

[0037] The subcritical water extraction treatment process is: take 5g solid sample and 3g diatomite, mix them uniformly and add them into a 10ml extraction pool for extraction treatment; wherein, the extraction solvent is water with 15% ethanol as a modifier, and the pH of the extraction solvent is 11. The extraction temperature was 110° C., and the extraction time was 10 minutes.

[0038] The DLLME process is as follows: add the extract obtained by subcritical water extraction into a conical centrifuge tube, and then add 150 microliters of chloroform as an extraction solvent. The centrifuge tube was vortexed at 1500 rpm for 10 seconds, then centrifuged at 6500 rpm for 8 min, and the chloroform at the tip of the centrifuge tube was recovered with a microsampling needle.

Embodiment 3

[0040] A method for extracting endocrine disruptors, wherein the sample to be tested is subjected to subcritical water extraction and then DLLME processing; details are as follows:

[0041] The subcritical water extraction treatment process is as follows: Weigh 5g solid sample, 2g diatomaceous earth and 2g neutral alumina, mix them uniformly and add them to a 10ml extraction pool for extraction treatment; wherein, the extraction solvent is 10% acetonitrile as a modifier water, the pH of the extraction solvent is 10. The extraction temperature is 120° C., and the extraction time is 10 min.

[0042] The DLLME treatment process is as follows: add the extract obtained by subcritical water extraction into a conical centrifuge tube, and then add 150 microliters of carbon tetrachloride as an extraction solvent. The centrifuge tube was vortexed at 1000 rpm for 30 seconds and then centrifuged at 6000 rpm for 10 min, and the chlorobenzene at the tip of the centrifuge tube was recovered...

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Abstract

The invention provides a method for extracting endocrine disruptors. The extraction method of the endocrine disruptor comprises the steps of firstly subjecting the sample to be tested to subcritical water extraction treatment, and then performing dispersion liquid-liquid micro-extraction treatment. The extraction method of endocrine disruptors of the present invention combines subcritical water extraction and dispersion liquid-liquid microextraction technology, so that the extraction method of endocrine disruptors of the present invention has high extraction efficiency for endocrine disruptors, and can effectively remove the influence of impurities, thereby realizing It can perform rapid and accurate qualitative and quantitative analysis of trace endocrine disruptors.

Description

technical field [0001] The invention belongs to the technical field of extraction, and in particular relates to an extraction method of endocrine disruptors. Background technique [0002] For nearly half a century, reports on the decline in the quality of human reproductive health and abnormal reproduction in wild animals have increased year by year: decreased sperm count and motility in men, increased incidence of breast cancer in women; increased hermaphroditism in wild cockroaches living downstream of sewage treatment plants in the UK Abnormal gonad development and sex hormone levels in crocodiles in Florida, USA; Feminization of male fish in polluted rivers, increased hermaphroditism, etc. People gradually realize that there are some compounds in the environment that can simulate hormone effects, regulate or interfere with the function of the biological endocrine system, namely endocrine disruptors (EDCs), which are defined as "can interfere with the maintenance of biolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34
Inventor 康海宁原珂栾天罡岳振峰王晓玮叶刚林黎涂小珂
Owner FOOD INSPECTION CENT OF CIQ SHENZHEN
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