Method for simultaneously detecting eight sterols in lucid ganoderma

A technology of sterols and Ganoderma lucidum, applied in the field of food and drug analysis, can solve the problems of weak ultraviolet absorption of sterols, false positive results, lack of scientific integrity, etc., and achieve the effects of strong specificity, high accuracy and high sensitivity

Active Publication Date: 2021-02-09
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the following problems exist in the quantitative analysis of sterol compounds in Ganoderma lucidum: There are many types of sterol compounds in Ganoderma lucidum, but in the existing quantitative methods, usually only the content of ergosterol is detected to evaluate the quality of Ganoderma lucidum. The method lacks scientific integrity; some sterols such as peroxyergosterol cannot be detected with a UV detector due to weak UV absorption; in addition, some sterols may co-elute with weak polar substances such as fatty acids with similar polarity, making the determination results false positive

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  • Method for simultaneously detecting eight sterols in lucid ganoderma
  • Method for simultaneously detecting eight sterols in lucid ganoderma
  • Method for simultaneously detecting eight sterols in lucid ganoderma

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Embodiment 1

[0064] The establishment of embodiment 1 detection method

[0065] 1. Sample pretreatment step: 0.50 g of Ganoderma lucidum fruiting body (or mycelium, or spore powder, strain source is Ganodermalucidum, Ganoderma lingzhi, Ganoderma leucocontextum and Ganoderma resinaceum) sample, according to 1:30 (weight to volume ratio, weight g: volume ml) of solid-liquid ratio Add methanol 15mL, after ultrasonic extraction for 60min, take the supernatant, pass through a 0.20μm organic filter membrane and dilute 10 times (10 times volume) with mass spectrometry grade methanol as the sample solution to be tested.

[0066] Among them, the preparation of the mixed standard solution: Take eight sterol compound standard products, accurately weigh 1.00 mg and dissolve them in mass spectrometry grade methanol, prepare a 1 mg / mL single standard sample solution, and then dilute to 0.5 mg / mL with mass spectrometry grade methanol respectively. mL, take 100 μL each and mix and dilute to 50 μg / mL mixed...

Embodiment 2

[0084] Methodological verification and result detection: methodological verification refers to the laboratory quality control specification food physical and chemical testing standards and the relevant provisions of the Pharmacopoeia.

[0085] 1. Precision: Prepare a mixed standard solution of eight sterol compounds, repeat the injection 6 times in the same day, calculate the concentrations of the eight sterols obtained in 6 experiments according to the standard curve, and calculate the intraday precision. Take the mixed standard solution and dilute it twice, inject samples twice a day for three consecutive days, and calculate the day-to-day precision based on the results of 6 experiments. The results showed that the RSDs of the intra-day precision and inter-day precision of the eight sterol compounds were less than 15.00%, which indicated that the method had good intra-day and inter-day precision. The specific results are shown in Table 2 and Table 3, respectively.

[0086] ...

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Abstract

The invention provides a method for simultaneously detecting eight sterols in lucid ganoderma. The method comprises the following steps: a lucid ganoderma sample pretreatment step, an ultra-high performance liquid chromatography detection step, a compound mass spectrum information acquisition step, an ultra-high performance liquid chromatography triple quadrupole mass spectrum combined analysis method establishment step and a data analysis step. The method for simultaneously detecting the eight sterols in the lucid ganoderma can be used for carrying out accurate qualitative and quantitative analysis on eight sterols compounds in sporocarp, mycelia, spore powder and related products of the lucid ganoderma, is high in accuracy, high in sensitivity and strong in specificity, and overcomes thedefects of the conventional chemical method and high performance liquid chromatography.

Description

technical field [0001] The invention relates to the field of food and drug analysis, in particular to a method for simultaneously detecting eight sterols in Ganoderma lucidum. Background technique [0002] Ganoderma lucidum is the collective name of multiple species under the genus Ganoderma sp. of Polyporaceae, among which Ganoderma lucidum (Ganoderma lucidum or Ganoderma lingzhi), white flesh Ganoderma lucidum (Ganodermaleucocontextum), sessile Ganoderma lucidum (Ganoderma resinaceum) and other species, in the folk It has a long history of being used as a medicinal material, and it has been confirmed by modern pharmacological research that it has various effects such as improving immunity, lowering blood fat, anti-tumor, and protecting the liver. It is an ideal source of raw materials for health care products. The fruiting bodies, mycelia and spores of Ganoderma lucidum contain a variety of sterol compounds, which together with triterpenoids constitute the material basis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/88G01N30/8679G01N2030/884G01N2030/027
Inventor 张劲松冯娜岳亚文周帅唐庆九王金艳冯杰唐传红刘艳芳谭怡
Owner SHANGHAI ACAD OF AGRI SCI
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