Method for determining residues of metabolites of four nitrofurans drugs in animal glue traditional Chinese medicine

Abstract

The invention discloses a method for determining residues of metabolites of four nitrofurans drugs in an animal glue traditional Chinese medicine. The method comprises the following steps: preparing to-be-tested sample solution; preparing standard solution; injecting a standard series of working solution into a liquid chromatograph / mass spectrometer, determining appearance positions of the metabolites of the four nitrofurans drugs and qualitative and quantitative determination ion pairs thereof, and making a standard curve equation by taking the abundance of the quantitative ion pairs as a vertical coordinate and taking the concentration as a horizontal ordinate; substituting data obtained by corresponding detection for the to-be-tested sample solution into the standard curve equation forcalculation, so as to obtain the contents of the metabolites of all the nitrofurans drugs in the to-be-tested sample. The metabolites of the four nitrofurans drugs are: a furanzolidon metabolite (AOZ), a furaltadone metabolite (AOMZ), a furacilin metabolite (SEM) and a furadantin metabolite (AHD).

Description

technical field [0001] The invention relates to a dispersive solid-phase extraction combined with pass-through solid-phase extraction purification-ultra-high performance liquid chromatography-tandem mass spectrometry detection method suitable for four nitrofuran drug metabolite residues in animal glue traditional Chinese medicines. Background technique [0002] Nitrofuran drugs are a class of synthetic antibiotics with a 5-nitrofuran core structure, mainly including furazolidone (FZD), nitrofurantoin (NFT), nitrofurazone (NFZ), and furaltadone (FTD). [0003] Nitrofuran drugs have a broad-spectrum inhibitory and killing effect on bacteria, and have effects on a large number of Gram-positive and negative bacteria, fungi, and protozoa; Nitrofuran drugs have chronic toxicity and can cause gastrointestinal reactions, such as Nausea, vomiting, anorexia, diarrhea, etc. can also cause hemolytic anemia and jaundice; long-term or high-dose use of nitrofuran drugs can produce toxic ef...

AI Technical Summary

Problems solved by technology

Traditional Chinese medicine glue is an important part of the traditional medicine treasure house of the motherland. It has a long history of use, rich sources of raw materials, complicated production processes, and extensive clinical applications. There is a large difference, and the detection of residues of nitrofuran drug metabolites in animal glue traditional Chinese medicine is very difficult

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