Fast detection method for surface enhanced Raman scattering of trace pesticide residues in oranges

A surface-enhanced Raman and pesticide residue technology, which is applied in Raman scattering, preparation of test samples, material excitation analysis, etc., can solve the problem of pesticide residue detection method precision and reproducibility without further verification and poor reproducibility, unable to achieve rapid detection of pesticide residues, etc., to achieve rapid and accurate qualitative and quantitative analysis, fast detection speed, and good consistency

Inactive Publication Date: 2014-10-15
JIANGXI AGRICULTURAL UNIVERSITY
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Problems solved by technology

Surface-enhanced Raman spectroscopy detection technology can theoretically achieve single-molecule detection, but citrus and other fruits contain a large number of macromolecular substances such as organic acids, pigments, colloids, and cellulose, which are prone to fluorescence, and different agricultural product matrix components Different, the interference to the SERS detection of pesticide molecules is also different; in addition, there are many types of pesticides used in agricultural products, and the Raman enhancement effect is quite different, resulting in the accuracy and reproducibility of the SERS method for detecting trace pesticide residues in agricultural products. poor
The method disclosed above uses surface-enhanced Raman spectroscopy to achieve the purpose of enhancing the signal of pesticide molecules in fruits, but does not remove the influence of matrix substances. The detection limit of the method needs to be further improved, and the accuracy and stability of the method are poor. The purpose of rapid detection of trace pesticide residues in fruits cannot be achieved
In addition, the precision and reproducibility of the method for detecting pesticide residues in fruits disclosed above have not been further verified.

Method used

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  • Fast detection method for surface enhanced Raman scattering of trace pesticide residues in oranges
  • Fast detection method for surface enhanced Raman scattering of trace pesticide residues in oranges
  • Fast detection method for surface enhanced Raman scattering of trace pesticide residues in oranges

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Rapid detection of triazophos pesticide residues in navel orange pulp by surface-enhanced Raman spectroscopy

[0037] (1) Preparation of silver nano-reinforced substrate: Pour a certain concentration of silver nitrate solution (36mg silver nitrate dissolved in 200mL ultrapure water) into a flask, place it on a constant temperature magnetic stirrer, heat it to boiling rapidly at high temperature, and within 2 minutes Gradually drop a 1% trisodium citrate solution (60mg trisodium citrate dissolved in 6mL ultrapure water) while stirring at a speed of 200r / min. At this time, the solution slowly changes from transparent to light brown. After 25 minutes of reaction, A gray-green liquid was obtained. After cooling down at room temperature, pour the above appropriate amount of silver colloid solution into a centrifuge tube, pour off a small amount of supernatant after centrifugation, then add an appropriate amount of ultrapure water to the centrifuge tube, and mix we...

Embodiment 2

[0048] Example 2: Rapid detection of triazophos pesticide residues in navel orange peel by surface-enhanced Raman spectroscopy

[0049] (1) Preparation of silver nano-reinforced substrate: Pour a certain concentration of silver nitrate solution (36mg silver nitrate dissolved in 200mL ultrapure water) into a flask, place it on a constant temperature magnetic stirrer, heat it to boiling rapidly at high temperature, and within 2 minutes Gradually drop a 1% trisodium citrate solution (60mg trisodium citrate dissolved in 6mL ultrapure water) while stirring at a speed of 200r / min. At this time, the solution slowly changes from transparent to light brown. After 25 minutes of reaction, A gray-green liquid was obtained. After cooling down at room temperature, pour the above appropriate amount of silver colloid solution into a centrifuge tube, pour off a small amount of supernatant after centrifugation, then add an appropriate amount of ultrapure water to the centrifuge tube, and mix we...

Embodiment 3

[0059] Example 3: Rapid detection of thiabendazole pesticide residues in navel orange pulp by surface-enhanced Raman spectroscopy

[0060] (1) Preparation of gold nano-reinforced substrate: Pour a certain concentration of perchlorauric acid solution (10mg perchlorauric acid dissolved in 100mL ultrapure water) into a three-necked flask, place it on a constant temperature magnetic stirrer, and control the temperature at 120 After heating at a constant temperature until boiling, quickly add a certain concentration of trisodium citrate solution (20mg trisodium citrate dissolved in 4mL ultrapure water), while stirring rapidly at a speed of 100r / min. The color of the prepared gold sol is wine red. After the solution is cooled, store it away from light.

[0061] (2) Select a Raman spectrometer with an excitation light source wavelength of 785nm, a power of 200mW, and a spectral scanning range of 400-2000cm -1 , with a resolution of 4cm -1 , the integration time is 10s, and the ave...

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Abstract

The invention discloses a fast detection method for the surface enhanced Raman scattering of trace pesticide residues in oranges, and belongs to the technical field of fast pesticide detection. The fast detection method comprises the following steps: adopting acetonitrile, anhydrous magnesium sulfate and sodium chloride for mixing in a certain ratio so as to extract pesticide ingredients from the oranges as many as possible; mixing anhydrous magnesium sulfate, N-propyl ethylenediamine, C18 and graphitized carbon in a certain ratio; eliminating influences of fluorescent substances, such as organic acids, pigments, pectin and cellulose, in an extraction liquid. According to the fast detection method, the detection accuracy for the surface enhanced Raman scattering of the pesticide residues in the oranges is greatly increased, the aim of the fast detection of the trace pesticide residues is achieved, and the fast detection method basically meets the requirements of detection limit specified by the state; the fast detection method is adopted to detect the trace pesticide residues in the oranges, is simple in pre-processing of samples to be detected, fast in detection speed, low in detection cost and high in accuracy and precision, can fast and accurately analyze the trace pesticide residues in the oranges in a qualitative and quantitative manner, and can be used for on-site sample detection on a large scale.

Description

technical field [0001] The invention relates to the field of rapid screening methods for trace pesticide residues in citrus, in particular to a surface-enhanced Raman spectrum rapid screening method for trace pesticide residues in citrus, and belongs to the technical field of rapid detection of pesticides in fruits and vegetables. Background technique [0002] Citrus is rich in flavonoids, carotenoids, vitamins and other nutrients, which have the effects of improving immunity, anti-oxidation, anti-aging, and lowering blood sugar. Citrus is the largest fruit in southern my country and the largest fruit in the world, occupying a very important position in the world agricultural economy. At present, pesticide control is still the most convenient, effective and economical way to control citrus diseases, insect pests and weeds, but the non-standard use of pesticides has caused serious pesticides in citrus to exceed the standard. [0003] At present, the detection methods of pest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 陈金印刘木华吴瑞梅王晓彬李俊杰蔺磊范苑
Owner JIANGXI AGRICULTURAL UNIVERSITY
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