Quick detecting method for sudan red in food

A detection method, Sudan red technology, applied in the field of rapid detection of Sudan red in food, can solve the problems of expensive equipment, complicated operation, difficult to popularize, etc., and achieve the effect of convenient operation, simple equipment and high extraction rate

Inactive Publication Date: 2014-10-15
GUANGXI UNIV
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AI-Extracted Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to: in view of the above-mentioned existing problems, provide a kind of rapid detection method of Sudan red in food, solve the problems suc...
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Abstract

The invention discloses a quick detecting method for sudan red in food, and relates to the field of food safety. The detecting method comprises the four steps of sample pretreatment, concentration and evaporating to dryness, film chromatography, and HPLC determination. The quick detecting method solves the problems that detecting instrument is expensive, complex to operate and not easy to popularize, has the characteristics of high extraction rate, high purifying purity, high recovery ratio and the like, and can achieve the purpose of being quick and reliable, simple and convenient to operate, and cost-saving.

Application Domain

Technology Topic

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  • Quick detecting method for sudan red in food
  • Quick detecting method for sudan red in food
  • Quick detecting method for sudan red in food

Examples

  • Experimental program(6)
  • Effect test(1)

Example Embodiment

[0020] Example 1
[0021] Weigh 10 g of red pepper powder into a centrifuge tube, add 30 mL of water for dilution, add 10 mL of a mixed solvent of n-hexane and acetone with a volume ratio of 5:3, shake for 1 min, extract by ultrasonic for 15 min, centrifuge at 3500 r/min for 1 min, filter, The residue was extracted twice with 10 mL and 5 mL of a mixed solvent of n-hexane and acetone with a volume ratio of 5:3, respectively, and the extracts were combined; the extract was placed in a distillation flask, and the distillation flask was placed in a 40°C water bath for rotary evaporation, Or add anhydrous sodium sulfate to the bottle for dehydration, filter and evaporate to dryness naturally; add 1 mL of acetone to dissolve the obtained in the previous step, pass through a polyamide thin-layer plate, and use chloroform:acetone:acetic acid=6:1:1 as a developing agent, The obtained crystals were dried and placed in a sampling bottle; acetonitrile was added to the upper sampling bottle to make the volume to 5 mL, shaken for 10 min, and allowed to stand for 20 min. % Formic acid:acetonitrile=80:20 as mobile phase, column temperature at 28°C, flow rate of 1.0ml/min, constant flow, and detection wavelength of 230nm.

Example Embodiment

[0022] Example 2
[0023] Weigh 5g of ketchup into a centrifuge tube, add 20mL of water for dilution, add 10mL of a mixed solvent of n-hexane and acetone with a volume ratio of 5:3, shake for 1min, extract by ultrasonic for 15min, centrifuge at 5000r/min for 1min, filter, and remove the residue. Extract twice with 10mL and 5mL of a mixed solvent of n-hexane and acetone with a volume ratio of 5:3 respectively, and combine the extracts; place the extracts in a distillation flask, and place the distillation flask in a 40°C water bath for rotary evaporation, or Add anhydrous sodium sulfate to the bottle for dehydration, filter and evaporate to dryness naturally; add 1 mL of acetone to dissolve the result obtained in the previous step, pass through a polyamide thin-layer plate, and use chloroform:acetone:acetic acid=6:1:1 as a developing agent, the obtained The crystals were dried and placed in a sampling bottle; acetonitrile was added to the upper sampling bottle to make the volume to 5 mL, shaken for 10 minutes, and allowed to stand for 20 minutes. Formic acid:acetonitrile=80:20 was used as the mobile phase, the column temperature was 28°C, the flow rate was 1.0ml/min, the constant flow, and the detection wavelength of 230nm.

Example Embodiment

[0024] Example 3
[0025] Weigh 20g of chili oil in a centrifuge tube, add 25mL of water for dilution, add 30mL of a mixed solvent of n-hexane and acetone with a volume ratio of 5:3, shake for 1min, extract by ultrasonic for 15min, centrifuge at 5000r/min for 1min, filter, and remove the residue. Extract twice with 10mL and 5mL of a mixed solvent of n-hexane and acetone with a volume ratio of 5:3 respectively, and combine the extracts; place the extracts in a distillation flask, and place the distillation flask in a 40°C water bath for rotary evaporation, or Add anhydrous sodium sulfate to the bottle for dehydration, filter and evaporate to dryness naturally; add 1 mL of acetone to dissolve the result obtained in the previous step, pass through a polyamide thin-layer plate, and use chloroform:acetone:acetic acid=6:1:1 as a developing agent, the obtained The crystals were dried and placed in a sampling bottle; acetonitrile was added to the upper sampling bottle to make the volume to 5 mL, shaken for 10 minutes, and allowed to stand for 20 minutes. Formic acid:acetonitrile=80:20 was used as the mobile phase, the column temperature was 28°C, the flow rate was 1.0ml/min, the constant flow, and the detection wavelength of 230nm.
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Description & Claims & Application Information

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