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Method for determining content of polysaccharide in cassia twig and poria cocos capsule

A technique of Poria cocos and a determination method, which are applied in the preparation of samples for testing, analysis of materials by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of complex composition of Poria cocos Less research on polysaccharide components, mutual interference of matrix or other effective components in the detection process, etc., to achieve the effect of good reproducibility, perfect quality control and strong specificity

Pending Publication Date: 2022-08-05
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the polysaccharide components of the whole compound Guizhi Fuling capsule
At present, there is no research on the content determination of polysaccharides in Guizhi Fuling Capsules, which is not enough to fully evaluate the quality of Guizhi Fuling Capsules
[0005] Moreover, the composition of Cinnamon Twig Poria cocos is complex, and the detection process often has the situation that the matrix or other active ingredients interfere with each other, so it is necessary to find a suitable method that can accurately detect Cinnamon Twig Poria cocos polysaccharides

Method used

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  • Method for determining content of polysaccharide in cassia twig and poria cocos capsule
  • Method for determining content of polysaccharide in cassia twig and poria cocos capsule
  • Method for determining content of polysaccharide in cassia twig and poria cocos capsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Establishment of a method for the determination of polysaccharide content in Guizhi Fuling Capsules

[0063] The present invention utilizes the anthrone-sulfuric acid method to measure the content of polysaccharides. In this section, the main factors affecting the color reaction result are selected for investigation, including: sulfuric acid concentration in the color developer, maximum absorption wavelength, water bath heating color development time and color development time After the stability of the solution, the optimal color development conditions were finally determined.

[0064] 1 Experimental materials and instruments

[0065] 1.1 Reagents and reagents

[0066] Guizhi Fuling Capsules (Jiangsu Kangyuan Pharmaceutical Co., Ltd., batch number 171001); Anhydrous glucose (China Institute for Food and Drug Control, batch number 110833-201707, purity ≥99.9%); Anthrone (AR, Sinopharm Chemical Reagent Co., Ltd. Company, batch number: 20180815); sodium hydrox...

Embodiment 2

[0110] Embodiment 2 Determination of water-soluble polysaccharide content in Guizhi Fuling Capsules

[0111] 1 Experimental materials and instruments

[0112] 1.1 Reagents and reagents

[0113] Guizhi Fuling Capsules (Jiangsu Kangyuan Pharmaceutical Co., Ltd., batch number 171001, 180901, 180902, 181001); Anhydrous glucose (China Institute for Food and Drug Control, batch number 110833-201707, purity ≥ 99.9%); Anthrone (AR , Sinopharm Group Chemical Reagent Co., Ltd., batch number: 20180815); concentrated sulfuric acid (AR, Nanjing Chemical Reagent Co., Ltd., batch number: 190128026J); anhydrous ethanol (AR, Nanjing Chemical Reagent Co., Ltd., batch number: 190214148k); water is Ultrapure water (made in laboratory).

[0114] 1.2 Experimental instruments

[0115] UV-2401PC UV-Vis Spectrophotometer (Shimadzu Corporation, Japan); KH-500DE CNC Ultrasonic Cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.); BY-320A Desktop Centrifuge (Beijing Baiyang Medical Instrument Co...

Embodiment 3

[0215] Embodiment 3 Determination of alkali-soluble polysaccharide content in Guizhi Fuling Capsules

[0216] The residue after extraction by the preparation of the water-soluble polysaccharide test solution in the previous step still contains a large amount of polysaccharides, but it may be due to the strong intermolecular hydrogen bonding force in its structure or a part of acidic polysaccharides and relatively large molecular weight. Polysaccharides, resulting in poor solubility in pure water, only measuring water-soluble polysaccharides can not truly reflect the actual situation of the polysaccharides in Guizhi Fuling capsules. Moreover, previous experiments found that the direct measurement of the content of alkali-soluble polysaccharides was disturbed by water-soluble polysaccharides, and the experimental results were not stable enough. Therefore, further investigation is needed to improve the quality control standard of Guizhi Fuling Capsules. Consider pre-treatment wi...

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Abstract

The invention discloses a method for determining the content of polysaccharide in a cassia twig and poria cocos capsule, the polysaccharide comprises water-soluble polysaccharide, and the method comprises the following steps: obtaining a cassia twig and poria cocos capsule water-soluble polysaccharide sample to be detected; determining the water-soluble polysaccharide of the cassia twig and poria cocos capsule water-soluble polysaccharide sample to be detected by using an anthrone-sulfuric acid colorimetric method; wherein the step of obtaining the to-be-detected water-soluble polysaccharide sample of the cassia twig and poria cocos capsule comprises the following steps: taking contents of the cassia twig and poria cocos capsule, uniformly mixing, grinding, removing impurities, extracting, and recovering the water-soluble polysaccharide from an extracting solution, so as to obtain the to-be-detected water-soluble polysaccharide sample; wherein the impurity removal comprises the following steps: adding 70%-80% ethanol, carrying out ultrasonic treatment for 1-5 times, and carrying out ultrasonic treatment for 15-25 minutes each time; the extraction comprises the following steps: an extraction mode is selected from ultrasonic treatment, warm immersion or reflux, an extraction solvent is water, the extraction time is 15-120 minutes, and the number of times of extraction is 1-3; the step of recovering the water-soluble polysaccharide from the extracting solution comprises the following steps: concentrating the extracting solution to 3-5mL, adding absolute ethyl alcohol until the alcohol content is 60-80%, and refrigerating and standing for 6-24 hours.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicine preparations, in particular to a method for determining the content of polysaccharide components in Guizhi Fuling capsules. Background technique [0002] Guizhi Fuling Capsule [Z10950005] is a compound preparation refined by modern technology from 5 traditional Chinese medicines of Guizhi, Poria, Cortex Moutan, White Peony and Peach Kernel. Modern pharmacological studies have shown that Guizhi Fuling Capsule has pharmacological effects such as anti-inflammatory, analgesic, anti-tumor, regulating smooth muscle, regulating endocrine and improving immunity. It is clinically used to treat uterine fibroids, pelvic inflammatory disease, dysmenorrhea, endometrium Gynecological diseases such as heterotopia. At present, the quality control of Guizhi Fuling Capsules mainly focuses on the research of active small molecular compounds such as phenolic acids, triterpenes, glycosid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/28G01N1/34
CPCG01N21/78G01N1/28G01N1/34
Inventor 肖伟杨彪胡玉梅李璐顾莎莎刘文君曹亮王振中
Owner JIANGSU KANION PHARMA CO LTD
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