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Methylene blue colorimetric method for determining concentration of gamma-PGA in solution

A methylene blue, -PGA technology, applied in the measurement of color/spectral characteristics, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve methylene blue interference, glutamic acid interference, etc. problem, to achieve the effect of simple operation and solving the interference of methylene blue

Inactive Publication Date: 2020-04-03
DONGGUAN UNIV OF TECH
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Problems solved by technology

[0006] The purpose of the present invention is to provide a methylene blue colorimetric method for measuring the concentration of γ-PGA in solution, which solves the problem of glutamic acid interference when measuring the concentration of γ-PGA by ultraviolet spectrophotometry, and also improves the methylene blue colorimetric method for determining unknown solutions. Medium γ-PGA concentration, while solving the problem of methylene blue interference in the determination of γ-PGA concentration by methylene blue colorimetry, convenient, fast, accurate and reliable, and can be used for the determination of γ-PGA concentration in unknown solutions

Method used

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  • Methylene blue colorimetric method for determining concentration of gamma-PGA in solution
  • Methylene blue colorimetric method for determining concentration of gamma-PGA in solution

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Embodiment 1-28

[0020] The reagent used in embodiment 1-28 is the gamma-PGA standard solution of 1g / L, the methylene blue standard solution and pure water of 1g / L, and the consumption of each embodiment is as follows:

[0021]

[0022] The specific operation steps of embodiment 1-28 are as follows:

[0023] The first step, at 25°C, accurately measure the corresponding amount of 1g / L γ-PGA standard solution into the test tube, then add the corresponding amount of ultrapure water, shake and mix, adjust the pH = 6.5, and then add 40 μL Concentration of 1g / L methylene blue standard solution, shake and mix, reaction time 3h.

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Abstract

The invention relates to a methylene blue colorimetric method for determining the concentration of gamma-PGA in a solution. The methylene blue colorimetric method comprises the following steps: (1) preparing a methylene blue and gamma-PGA aqueous solution; (2) methylene blue and a gamma-PGA aqueous solution are subjected to a mixed reaction; (3) carrying out full-wavelength scanning on the mixed solution of methylene blue and gamma-PGA at the wavelength of 200-800nm; (4) judging the type of a full-wavelength scanning spectrum; and (5) calculating the gamma-PGA concentration according to the standard curve. The problem of glutamic acid interference when the gamma-PGA concentration is measured by an ultraviolet spectrophotometric method is solved. The gamma-PGA concentration in the unknown solution is determined by using the methylene blue colorimetric method, the problem of methylene blue interference when the gamma-PGA concentration is determined by using the methylene blue colorimetric method is solved, and the method is convenient, rapid, accurate and reliable and can be used for determining the gamma-PGA concentration in the unknown solution.

Description

technical field [0001] The invention provides a methylene blue colorimetric method for measuring the concentration of γ-PGA in a solution, which belongs to a spectrophotometric detection method in the field of biotechnology, and can be used to measure solution samples with unknown γ-PGA content, especially in γ-PGA. Screening of high-yielding strains of PGA and monitoring during fermentation and detection of PGA-rich solid substrates. Background technique [0002] Polyglutamic acid (γ-PGA) is mainly a kind of extracellular water-soluble natural high molecular amino acid polymer produced by Bacillus fermentation, composed of D-glutamic acid (D-Glu) or L-glutamic acid (L- Glu) is a polypeptide molecule linked by an amide bond between the α-amino group and the γ-carboxyl group, and the γ-PGA synthesized by microorganisms is usually composed of about 5,000 glutamic acid monomers, and its relative molecular mass is between 100- Between 1000kD. [0003] Due to the active functio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 邱华贤胡思婷邹水洋郭俊贤
Owner DONGGUAN UNIV OF TECH
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