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A method for the determination of volatile fatty acids in tobacco leaves

A technology of volatile fatty acid and determination method, applied in the field of tobacco chemical component analysis, can solve the problems of non-volatile fatty acid determination, loss of volatile components, etc., and achieve the effects of simple and convenient detection process, high sensitivity and good reproducibility

Active Publication Date: 2021-06-18
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for the determination of volatile fatty acids in tobacco leaves, so as to solve the problem that the existing determination methods will cause the loss of volatile components and cannot comprehensively and accurately measure the volatile fatty acids in tobacco leaf samples

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  • A method for the determination of volatile fatty acids in tobacco leaves
  • A method for the determination of volatile fatty acids in tobacco leaves
  • A method for the determination of volatile fatty acids in tobacco leaves

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Embodiment 1

[0033] The assay method embodiment 1 of volatile fatty acid in tobacco leaf of the present invention, chooses flue-cured tobacco C3F sample, adopts the following steps:

[0034] 1) Crush the tobacco leaves and pass through a 40-mesh sieve, weigh 0.5g of tobacco leaf powder and add 3mL of CH 2 Cl 2 As an extraction solvent, 50 μL of trans-2-hexenoic acid internal standard solution with a concentration of 20 μg / mL was added, vortexed at 2500 rpm for 30 min, and then filtered with a 0.45 μm filter membrane to obtain an extract.

[0035] 2) Take 1.0 mL of the extract in a chromatographic bottle, add 200 μL of derivatization reagent (N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA)) and shake evenly, and derivatize in a water bath at 60°C for 30 minutes. After cooling to room temperature, it was directly detected by GC-MS / MS.

[0036] GC-MS / MS analysis conditions are as follows: DB-5MS (60m×0.25mm×0.25μm) capillary column separation, inlet temperature: 280°C, injection volume 1μ...

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Abstract

The invention relates to a method for determining volatile fatty acids in tobacco leaves. The determination method comprises the following steps: 1) adding dichloromethane to the tobacco leaf sample for extraction, and separating to obtain an extract; 2) adding a derivatization reagent to the extract, and performing gas chromatography-tandem mass spectrometry after the derivatization reaction. The method for measuring volatile fatty acids in tobacco leaves provided by the present invention extracts tobacco leaf samples with dichloromethane at normal temperature, can effectively extract C1-C10 volatile fatty acids, and perform detection and analysis after derivatization reaction; the whole measurement process has few steps , The process is compact, reducing the loss of volatile components, and realizing the Determination of 17 kinds of volatile fatty acids such as ‑methylvaleric acid and 4‑methylvaleric acid, the determination results of volatile fatty acids in tobacco leaves are more comprehensive and accurate.

Description

technical field [0001] The invention belongs to the field of tobacco chemical component analysis, and in particular relates to a method for measuring volatile fatty acids in tobacco leaves. Background technique [0002] Tobacco leaves contain a large amount of fatty acids, accounting for about 10% of their dry weight. Fatty acid has a unique aroma and acidity, which is not only an important part of the aroma quality and style characteristics of cigarettes, but also can change the acid-base balance of smoke, and then have a significant impact on the strength, irritation and taste of cigarettes. According to the strength of volatility, fatty acids can be divided into volatile, semi-volatile and non-volatile fatty acids. Usually, volatile fatty acids refer to lower fatty acids below C10, which account for about 0.1%-0.2% of the dry weight of tobacco leaves . [0003] Volatile fatty acids have a great impact on the sensory quality of tobacco, for example: C1-C3 fatty acids hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 薛聪王冰余晶晶张晓兵蔡君兰赵晓东刘克建秦亚琼刘绍锋刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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