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Method for determining phenylpropanoid glycoside substances in Tibetan medicine Lamiophlomis rotata

A phenylpropanoid glycoside, unique technology, applied in the field of analytical chemistry, can solve the problems of limitation, no reference substance determination, difficult preparation, etc., and achieve the effects of easy standardization, good application value, and easy availability.

Inactive Publication Date: 2012-04-04
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the labor-intensive and time-consuming work of purifying and preparing the phenylpropanoid reference substance from the unique plant, the cost is high, and the preparation of some low-content phenylpropanoid reference substances in the unique plant is very difficult, so this The determination method is often limited by the absence of a reference substance. Usually, only a few phenylpropanoid glycosides in Duyiwei can be measured, which cannot fully reflect the content of phenylpropanoid glycosides in Duyiwei.

Method used

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  • Method for determining phenylpropanoid glycoside substances in Tibetan medicine Lamiophlomis rotata
  • Method for determining phenylpropanoid glycoside substances in Tibetan medicine Lamiophlomis rotata

Examples

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Effect test

Embodiment 1

[0039] Sample pretreatment: Accurately weigh 10.0g of crushed Duyiwei Whole Herb into a 500ml flask, and extract with 100ml of distilled water under reflux for a total of 3 extractions. The extracts obtained for 3 times were combined, filtered with gauze, the filtrate was distilled and concentrated to 30ml with a rotary evaporator, 10% hydrochloric acid was added for hydrolysis, the concentration of hydrochloric acid was adjusted to 2mol / L, hydrolyzed solution was obtained after 2 hours of hydrolysis, and the volume was determined after filtration. To 50ml, accurately measure 10ml, then dilute 10 times with methanol and dilute to a 100ml volumetric flask to obtain a unique whole herb sample solution for injection analysis.

[0040]Preparation of reference solution: 1.54 g of hydroxytyrosol with a purity of 98%; 1.68 g of homovanillyl alcohol with a purity of 95%; 1.82 g of 3,4-dimethoxyphenethyl alcohol with a purity of 95%; 96% caffeic acid 1.80g; 97% ferulic acid 1.94g; 95% ...

Embodiment 2

[0043] Sample pretreatment: Accurately weigh 6.72g of crushed unique flavor tablets into a 250ml flask, add 25ml of distilled water and 25ml of methanol for heating and reflux extraction, and extract for a total of 3 times. The extracts obtained for 3 times were combined, filtered with filter paper, the filtrate was distilled and concentrated to 20ml with a rotary evaporator, 10% hydrochloric acid was added for hydrolysis, the concentration of hydrochloric acid was adjusted to 2.5mol / L, and hydrolyzed solution was obtained after 1 hour of hydrolysis, which was determined after filtration. Volume to 50ml, accurately measure 10ml, and then dilute 10 times with methanol and dilute to a 100ml volumetric flask to obtain a unique tablet preparation sample solution for injection analysis.

[0044] Preparation of reference solution: 1.54 g of hydroxytyrosol with a purity of 98%; 1.68 g of homovanillyl alcohol with a purity of 95%; 1.82 g of 3,4-dimethoxyphenethyl alcohol with a purity ...

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Abstract

The invention discloses a method for determining phenylpropanoid glycoside substances in a Tibetan medicine Lamiophlomis rotate. According to the invention, the characteristic that phenylpropanoid glycoside can be hydrolyzed to release phenethanol and cinnamic acid compounds is used, various types of phenethanol and cinnamic acid compounds in Lamiophlomis rotata are separated by a high performance liquid chromatography method and determined by using an external standard method, so that accurate determination of phenylpropanoid glycoside substances in the Lamiophlomis rotate can be achieved.

Description

technical field [0001] The invention relates to a technology for separating and measuring phenylpropanoid glycosides in the unique Tibetan medicine and its preparation, and belongs to the technical field of analytical chemistry. Background technique [0002] It is uniquely a Lamiaceae plant, which grows in alpine meadows at an altitude of 2700-4500 meters, and is widely distributed in Tibet, Gansu, Sichuan and other plateau regions. It is a folk medicine for Tibetans, Mongolians and Naxi people. It has the functions of removing blood stasis, antibacterial and anti-inflammatory. It is mainly used in the clinical treatment of bruises, traumatic bleeding, rheumatic arthralgia, and yellow water disease. [0003] Unique plants mainly contain flavonoids, iridoids and phenylpropanoids. Chinese Patent No. 200510035066.5 provides an injection prepared by extracting and separating raw materials and containing phenylpropanoid glycosides as the main active ingredient, and relates to a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53G01N30/02G01N30/06A61P19/00A61P29/00A61P17/02A61P7/04A61P31/04
Inventor 赵亮董树清刘芹
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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