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Detection method for drug capable of treating fever in children

A detection method, a technology for children with fever, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not reflecting the internal quality of drugs, lack of comprehensiveness, etc., and achieve the effect of shortening the inspection cycle, good repeatability, and comprehensive product quality

Active Publication Date: 2014-11-26
GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] To sum up, it can be seen that traditional Chinese medicine compound preparations usually restrict and coordinate with each other by "lord, minister, assistant, and envoy", emphasizing that they play a role as a whole. The detection by a single component lacks comprehensiveness and cannot reflect the inherent quality of drugs

Method used

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  • Detection method for drug capable of treating fever in children
  • Detection method for drug capable of treating fever in children
  • Detection method for drug capable of treating fever in children

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0040] Embodiment 1-4 uses different chromatographic conditions to detect palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhizinate;

[0041] Take appropriate amount of 4 reference substances of palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhizinate respectively, put them in a 50mL volumetric flask, add methanol to make each 1mL contain 60μg of palmatine hydrochloride, 80μg of berberine hydrochloride, licorice A mixed solution of 70 μg of glycoside and 200 μg of ammonium glycyrrhizinate was used as a mixed reference solution.

[0042] Linghuang Baoer pills were crushed and passed through a No. 4 sieve, and 0.5g of fine powder was taken, accurately weighed, placed in a stoppered Erlenmeyer flask, accurately added with 25mL of methanol, weighed, treated with ultrasonic (480W, 40KHz) for 30min, let cool, added Make up the lost weight with methanol, shake well, filter with a microporous membrane (0.45 μm), and take...

Embodiment 5

[0054] This example provides an investigation of the linear range of the method.

[0055] Take 10 mg of liquiritin, 10 mg of palmatine hydrochloride, 12 mg of berberine hydrochloride, and 10 mg of ammonium glycyrrhizinate as reference substances, accurately weigh them, put them in the same 50 ml volumetric flask, add methanol to dissolve and dilute to the mark. Accurately draw reference substance solution 1ml, 2ml, 3ml, 5ml, 8ml, 10ml to 10ml volumetric flask, add methanol to dilute to the mark and shake well, according to the method provided in Example 1 of the present invention, sequentially inject and measure. Taking the determination of the peak area as the ordinate, and the concentration of the reference substance solution as the abscissa (μg / ml) to draw a standard curve, the results are shown in Table 3, Figure 2a , 2b , 2c, 2d.

[0056] Table 3 Regression equation, correlation coefficient and linear range of four kinds of reference substances

[0057]

[0058] ...

Embodiment 6

[0060] The precision test that present embodiment provides method:

[0061] Linghuang Baoer pills were crushed and passed through a No. 4 sieve, and 0.5g of fine powder was taken, accurately weighed, placed in a stoppered Erlenmeyer flask, accurately added with 30mL of methanol, weighed, treated with ultrasonic (480W, 40KHz) for 15min, let cool, added Make up the lost weight with methanol, shake well, filter with a microporous membrane (0.45 μm), and take the subsequent filtrate as the test solution.

[0062] Take the solution to be tested, according to the method provided in Example 1 of the present invention, continuously inject 6 times, 5 μ L each time, record the peak areas of palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhizinate, and calculate the relative standard deviation , the result is as shown in table 4, and the result shows, adopts the method provided by the invention to detect palmatine hydrochloride, berberine hydrochloride, l...

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Abstract

The invention discloses a detection method for a drug capable of treating fever in children. The detection method comprises the following steps of 1) weighing a palmatine hydrochloride reference substance, a berberine hydrochloride reference substance, a liquiritin reference substance and an ammonium glycyrrhetate reference substance, preparing a mixed solution of the reference substances with an alcohol solvent, smashing Linghuang Bao'er pills, mixing the smashed Linghuang Bao'er pills with the alcohol aqueous solution to obtain a test sample solution; 2) measuring the mixed solution of the reference substances with different concentrations, detecting through HPLC to obtain the peak area respectively, making a standard curve to obtain the standard curve of the mixed solution of the reference substances; and 3) detecting the test sample solution through HPLC to obtain the peak area of the test sample solution, and comparing with the standard curve to obtain the content of palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhetate in the test sample solution. The detection method overcomes one-sidedness that the quality of a drug is determined only by measuring one component, is comprehensive and reliable, and has the advantages of high accuracy, high precision, stability, and relatively good repeatability.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and relates to a detection method for a drug for treating fever in children, in particular to a method for simultaneously detecting the contents of four components in the drug, palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhizinate, by using HPLC method method, the drug refers to Linghuang Baoer pills. Background technique [0002] Linghuang Baoer Pill is a compound preparation of traditional Chinese medicine. It has the effects of clearing away heat and quenching wind, eliminating phlegm and calming convulsions, and awakening the mind. However, the current quality standard of Linghuang Baoer Pills has not yet included the relevant content determination methods. [0003] Prior art 1: The quality standard of Linghuang Baoer Pills is recorded in the 14th volume of traditional Chinese medicine preparations in the "Drug Standards of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
Inventor 王文楚严志标彭红英陆颂规曾利杰陈丽斯陈晓坚程君璐江涛杨滨宾
Owner GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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