43 results about "Palmatine hydrochloride" patented technology

The invention relates to novel application of berberine and derivatives thereof in preparation of medicines, in particular to application of berberine and derivatives thereof in preparation of indole amine 2, 3-dioxygenase IDO inhibitor, belonging to the medicine field. According to IDO inhibition activity detection, reversible inhibition judgment, inhibitor type judgment, Ki value determination and median effective inhibition concentration IC50 determination, the results show that berberine is reversible inhibitor and inhibition constant Ki is 8muM; and jatrorrhizine hydrochloride and palmatine hydrochloride are irreversible inhibitors, and the median effective inhibition concentrations IC50 thereof are respectively 123muM and 126muM. When the berberine and the derivatives thereof disclosed in the invention are used as IDO inhibitors, the application prospect is wide and the IDO inhibitors can be used for treating serious diseases such as cancers, AIDS, Alzheimer diseases, tristimania, cataract and the like with pathological feature of IDO-mediated tryptophan metabolism.

The present invention discloses a Rhizoma Corydalis Decumbentis total alkaloids extract, its preparation method, pharmaceutical composition containing same. The total alkaloids mainly comprise : macleyine, tetrahydropalmatine, bicucalline, palmatine hydrochloride, 'xiawuning' alkaloid, corydaline harmine, other alkaloid and extract. The preparation method is : using Rhizoma Corydalis Decumbentis as material, adding right amount of polar solvent for leaching, merging the leachate, carrying out large pore adsorption resin chromatography, eluting impurity with diluted acid and alkaline aqueous solution orderly, eluting using polar organic solvent, collecting elution liquor, eliminating impurity further by alumina column adsorption, Collecting after-column liquid, concentrating liquor to obtain the Rhizoma Corydalis Decumbentis total alkaloids extract. The present invention also discloses a pharmaceutical composition containing the total alkaloids extract and uses of the pharmaceutical composition in preparing tablet, capsule, soft capsule, suppository, granula, transdermal absorption agent, drop pills, oral disintegrating agent, slow release agent, freeze-drying powder injection etc.

The invention provides a total alkaloid of common fibraurea stems, which contains palmatine hydrochloride with the weight percentage content over 60 percent and jatrorrhizine hydrochloride with the weight percentage content more than 10 percent. The invention also provides a method for preparing the total alkaloid of common fibraurea stems, fibriuretinin and jatrorrhizine. The method can be adopted to prepare the total alkaloid of common fibraurea stems and to extract separate fibriuretinin and jatrorrhizine, wherein if dry products are calculated on the basis of anhydrous palmatine hydrochloride (C21H22O4N.HCl), the fibriuretinin has the weight percentage content higher than 98.0 percent and is high in purity. The extraction-separation method enables the transfer rate of the palmatine hydrochloride to be up to over 26 percent and is 1.7 times higher than the prior conventional process (about 15 percent). The extraction process also maintains the jatrorrhizine while raising the transfer rate of the palmatine hydrochloride serving as a main component of the fibriuretinin, thereby greatly improving the resource utilization rate of medicinal materials. The process adopts a resin purification method, is simple and feasible in process flow, capable of regenerating and repeatedly utilizing resin and reducing environmental pollution caused by lye compared with the conventional salting-out method.

The invention discloses a design of a comprehensive extraction process for barberry plant resources. Berbamine hydrochloride, jatrorrhizine hydrochloride, palmatine hydrochloride and a muscle relaxant are synchronously, efficiently and comprehensively extracted while berberine hydrochloride is extracted, and berbamine is used as a raw material for synthesizing an analgesic drug. Therefore, the barberry plant resources are fully utilized, the added medicinal value is increased, and the comprehensive utilization rate of medicinal barberry is increased. For a traditional Chinese medicine, one medicinal part is often utilized only and other parts are discarded. Various components in the barberry are synchronously extracted and comprehensively utilized, so that the economic value of the barberry is greatly increased.

The invention discloses a total alkaloid of Stephania delavayi Diels, the content of which is measured by tetrahydropalmatine. Tetrahydropalmatine occupies 55-95 percent of the total alkaloid extract of Stephania delavayi Diels, wherein the weight percentage of tetrahydropalmatine contained is no less than 2 percent, while the weight percentage of palmatine hydrochloride contained is no less than 0.8 percent. The invention also discloses a preparation method of the total alkaloid of Stephania delavayi Diels, which relates to the steps as follows: adding the Stephania delavayi Diels meal into ethanol for diacolation or reflux to acquire alcohol extract, decompressing and recycling the ethanol and concentrating into a condensed ointment for dissolving and filtrating with acid to acquire a filtrate, passing the filtrate through a macroporous resin column or a strong acid cation exchange resin column for adsorption, washing with water to remove the impurities and extracting with a eluent, collecting the eluent and decompressing and recycling the extract and then concentrating and drying, thus acquiring the total alkaloid of Stephania delavayi Diels; the steps can also be decompressing a percolating liquid to recycle the ethanol, concentrating, dissolving with acid and then filtrating, adjusting the pH value of the filtrate with weak base to produce deposition, filtrating the deposition to acquire a filter residue, adding ethanol into the filter residue to resolve and decompress, recycle the ethanol and then drying, thus acquiring the total alkaloid. The results of a pharmacological study on the acquired total alkaloid show that the total alkaloid of Stephania delavayi Diels has good effect on spasmolysis, pain relieving, antiphlogosis and antibiosis, blood activating and so on, and can combine with the acceptable components in pharmacy to prepare various sustained release or controlled release formulation or other formulations.

An application of the palmatine hydrochloride extracted from coptis root or phellodendron bark for treating diabetes B is disclosed.

The invention relates to a method for extracting and purifying total alkaloid of Chinese mahonia stem; wherein the total alkaloid can be used to prevent and treat gastric ulcer, is mainly composed of berberine hydrochloride, jatrorrhizine hydrochloride, palmatine hydrochloride, and other alkaloids, and can be solely used or cooperatively used with pharmaceutically-acceptable carriers to prepare drugs for preventing and treating gastric ulcer.

Contents of effective components, such as calycosin-7-glucoside, specnuezhenide, palmatine hydrochloride, berberine hydrochloride and rosmarinic acid, of a traditional Chinese medicine composition are measured at the same time by means of the UPLC method, the method is simple and quick and suitable for quality control of the traditional Chinese medicine composition, and reference is provided for dynamic real-time monitoring in continuous production of the traditional Chinese medicine composition.

The invention relates to a microbial secondary metabolite palmatine hydrochloride and application thereof and belongs to the technical field of biological pesticides. The secondary metabolite is prepared by the following steps: (a), producing a strain of L.antibioticus YFY02 with the collection number of CGMCC NO.2403; (b) culturing the strain YFY02 for two days by utilizing a KB culture medium, performing enlarged culture by adopting a conventional fermentation method, extracting the fermentation liquor by using ethyl acetate, and performing rotary evaporation, thereby obtaining a crude metabolite extract; (c) by utilizing liquid chromatography, Sephadex LH-20 gel column chromatography, mass spectrometry, nuclear magnetic resonance and other spectroscopic techniques, and separating, purifying and identifying the crude extract of the secondary metabolite; and (d) identifying to obtain the effective secondary metabolite palmatine hydrochloride. The invention also discloses application of the microbial secondary metabolite palmatine hydrochloride in the preparation of agents for preventing and controlling bacterial blight of rice, bacterial leaf streak and plasmodiophora brassicae woronin. The microbial secondary metabolite disclosed by the invention has the advantages of environment friendliness, low cost and excellent effects of preventing and controlling the rice bacterial diseases and plasmodiophora brassicae woronin.

The invention relates to a palmatine hydrochloride crystal form C as well as a preparation method thereof and an application thereof in a medicament composition or a health-care product. The invention also relates to the medicament composition or the health-care product containing a crystal, and an application of the crystal in preparing the medicament composition or the health-care product with anti-inflammation, anti-bacterium, pain alleviating and tranquilizing, anti-hypertension, cardiac arrhythmia prevention and anti-tumor effects.

The invention provides a method for performing extraction preparation on palmatine chloride from traditional Chinese medicines and application of the palmatine chloride to preparation of a medicine for preventing and treating colorectal cancer. According to the preparation method, an acid-alkali system solvent method is adopted, medicinal materials are pulverized and 0.1-2 percent of acid waterborne solvents are added for performing backflow and extraction; the solvents are treated by utilizing the acid-alkali system solvent method after being recycled to obtain palmatine chloride of which the purity is more than 85 percent. The palmatine chloride prepared by using the method has the advantages of high purity, short period and low cost, and is an environmental-friendly preparation method and suitable for industrial production.

Disclosed is externally used fibrauretine liquid for gynaecology and oral cavity, wherein the preparation essentially comprises fibrauretine as active constituent, and balancing adjuvant and water for preparing the externally used liquid preparation, the fibrauretine is alkaloid extracted from natural plant of fibraurea stem, calculated by dried product, it comprises Palmatine Hydrochloride C21H21O.HCl 20-98%. The dosage type includes externally used liquid lotion or liniment, foam aerosol, ointment, gargle or spray.

The invention provides a method for analyzing all components of an angelica sinensis six-yellow decoction, and belongs to the technical field of pharmaceutical analysis. An ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technology is adopted to detect an angelica sinensis six-yellow decoction sample, and 15 chemical reference substances are adopted at the same time: baicalin, wogonoside, berberine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, phellodendrine hydrochloride, astragaloside, calycosin-7-glucoside, ferulic acid, chlorogenic acid, ligustilide, verbascoside, baicalein and wogonin are adopted to confirm a detection result of the angelica sinensis six-yellow decoction andclearly identify 68 components; comprehensive analysis of the components of the angelica sinensis six-yellow decoction is realized, and the reliability of quality control is improved.

The invention discloses a detection method for a drug capable of treating fever in children. The detection method comprises the following steps of 1) weighing a palmatine hydrochloride reference substance, a berberine hydrochloride reference substance, a liquiritin reference substance and an ammonium glycyrrhetate reference substance, preparing a mixed solution of the reference substances with an alcohol solvent, smashing Linghuang Bao'er pills, mixing the smashed Linghuang Bao'er pills with the alcohol aqueous solution to obtain a test sample solution; 2) measuring the mixed solution of the reference substances with different concentrations, detecting through HPLC to obtain the peak area respectively, making a standard curve to obtain the standard curve of the mixed solution of the reference substances; and 3) detecting the test sample solution through HPLC to obtain the peak area of the test sample solution, and comparing with the standard curve to obtain the content of palmatine hydrochloride, berberine hydrochloride, liquiritin and ammonium glycyrrhetate in the test sample solution. The detection method overcomes one-sidedness that the quality of a drug is determined only by measuring one component, is comprehensive and reliable, and has the advantages of high accuracy, high precision, stability, and relatively good repeatability.

The invention provides an HPLC fingerprint spectrum of wine steamed coptidis rhizoma. The HPLC fingerprint spectrum is detected by adopting HPLC. A detection method comprises the steps of 1) preparinga reference solution; 2) preparing a test solution; and 3) sucking the reference solution and the test solution to be injected into a liquid chromatograph, and recording a chromatogram. According tothe detection method for the HPLC fingerprint spectrum of the wine steamed coptidis rhizoma, the established fingerprint spectrum has strong specificity and is strong in characteristic peak regularityand good in consistency; the detection method can be used as a quality evaluation method of the wine steamed coptidis rhizome, and also can be used for measuring the content of groenlandicine, columbamine, epiberberine, jatrorrhizine hydrochloride, coptisine hydrochloride, palmatine hydrochloride, berberine hydrochloride and / or berberrubine in the wine steamed coptidis rhizome; and the wine steamed coptidis rhizoma can be effectively identified.

The invention discloses a process method for alcohol extraction of palmatine. According to the process method, the palmatine extracting rate is increased, the extraction time is shortened, the production efficiency is improved, filtrate is easy to filter, the labor intensity is reduced, the use amount of acid water is reduced, and therefore, the use amount of salting out raw materials and the sewage treatment amount are reduced. According to the technical scheme, the process method comprises the following steps of: extracting common fibraurea stem powder with alcohol, combining filtrate, cooling, and filtering to remove insoluble impurities; drying the filtrate through steaming, and eluting residues after the steaming with acid liquid; filtering eluant, regulating the alkali degree, standing until the insoluble impurities are separated, filtering, adding sodium chloride into the filtrate, carrying out salting out, and filtering to obtain a crude product; and heating to dissolve the crude product into ethanol, immediately filtering to remove the insoluble impurities, after the filtrate is cooled, slowly adding concentrated hydrochloric acid, after palmatine hydrochloride is separated out, filtering, and drying, so as to obtain the product.

The invention discloses a refined rhizoma corydalis decumbentis total alkaloid product, a preparation method thereof, a pharmaceutical composition and application of the refined rhizoma corydalis decumbentis total alkaloid product and the pharmaceutical composition. The refined rhizoma corydalis decumbentis total alkaloid product comprises, by weight, 15%-40% of protopine, 10%-40% of tetrahydropalmatine, 0-10% of bicuculline, 0.01%-2% of palmatine hydrochloride, 2%-10% of kikemanine, 15%-30% of egenine and 1%-5% of other alkaloids and extracts. The more scientific and efficient, simple, convenient and feasible method for extracting high-purity rhizoma corydalis decumbentis total alkaloids is established, and HPLC (high performance liquid chromatography) purity of the prepared refined rhizoma corydalis decumbentis total alkaloid product is above 98%, so that pharmacology and efficacy of the rhizoma corydalis decumbentis total alkaloids can be given to play more safely and effectively.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an antibacterial drug as well as preparation and an application thereof. The invention providesthe antibacterial drug. The antibacterial drug comprises levofloxacin hydrochloride and palmatine hydrochloride. The antibacterial drug provided by the invention is high in antibacterial effect, notonly does not enhance the drug resistance of bacteria, but also can reverse the drug resistance of CRKP.

The invention belongs to the field of building materials, and specifically relates to an antibacterial coating and a preparation method thereof. The antibacterial coating is prepared from the following main raw materials in parts by weight: lupeol, palmatine hydrochloride, coptisine, bergenin, citronellol, rutin, ursolic acid, nanometer Chlorite-schist powder, titanium dioxide, trimethylolpropane,hydrogenated castor oil and manganese acetate. According to the invention, the lupeol, the palmatine hydrochloride, the citronellol, the rutin and the ursolic acid with antibacterial effects are added into the antibacterial coating to improve antibacterial properties of the coating and reduce propagation of bacteria on the surface of the coating; and after the antibacterial coating is coated ontothe surface of a building which is in an environment with easy propagation of bacteria, the building is insusceptible to mildewing.

The invention discloses an establishment method for a variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules. The method comprises the following steps of (1) preparing reference substance solutions: preparing reference substance solutions of berberine hydrochloride, palmatine hydrochloride, coptisine, jatrorrhizine hydrochloride, aesculin, aesculetin and pulchinenoside anemoside B4 respectively; (2) preparing a test sample solution: weighing the Chinese pulsatilla root decoction granules, extracting the Chinese pulsatilla root decoction granules and filtering an extracted solution by using a microporous membrane to obtain the test sample solution; (3) obtaining the fingerprint spectrum by determining with high performance liquid chromatography, wherein chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler by a chromatographic column; gradient elution is adopted; variable wavelengths are detected by ultraviolet light wavelengths; and (4) evaluating similarity: evaluating the fingerprint spectrum of the test sample by "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese medicine (Edition 2004A)". The method is simple, stable, high in precision and good in repeatability, and can identify the authenticity and merits of products accurately.

The invention discloses a palmatine hydrochloride-naringenin pharmaceutical eutectic crystal with a slow-release effect, and belongs to the technical field of pharmaceutical crystallization. The structure unit of the pharmaceutical eutectic crystal contains palmatine hydrochloride molecules and naringenin molecules, and the molar ratio of the palmatine hydrochloride molecules to the naringenin molecules is 1:1, wherein the molecular formula is [C21H22ClNO4].[C15H12O5]. According to the invention, the palmatine hydrochloride is mixed with the naringenin according to a ratio, and solvent evaporation, and stirring or grinding processing are carried out to obtain the palmatine hydrochloride pharmaceutical eutectic crystal. The preparation method disclosed by the invention is simple and feasible, and the yield of the eutectic crystal is high. In addition, a prepared palmatine hydrochloride-naringenin medicine has no hygroscopicity, is high in thermal stability and light stability, has a slow release effect on the palmatine hydrochloride, and can be applied to develop a palmatine hydrochloride slow-release preparation.

The invention provides a quality control method of a powerful dysentery relieving soluble powder preparation, and belongs to the field of the detection of medicines. The quality control method comprises the following steps of a first step, preparing the preparation; a second step, using berberine hydrochloride and palmatine hydrochloride as reference substances, and identifying whether ten great meritorious components are contained in powerful dysentery relieving soluble powder or not by thin layer chromatography; a third step, using cortex ilicis rotundae as a reference medicinal material and syringoside as a reference substance, and identifying whether a component of the cortex ilicis rotundae is contained in the powerful dysentery relieving soluble powder or not by the thin layer chromatography; a fourth step, using matrine and sophoridine as reference substances, and identifying whether a component of radix sophorae flavescentis is contained in the powerful dysentery relieving soluble powder or not by the thin layer chromatography; a fifth step, using the syringoside as the reference substance, and measuring the content of the cortex ilicis rotundae in a powerful dysentery relieving soluble powder formula by high performance liquid chromatography. The quality control method has the beneficial effects that an established quality standard control method has characteristics of high accuracy, good repeatability and the like. Meanwhile, the controllability of a quality standard of a preparation can be guaranteed; the inner quality and the curative effect of a product are guaranteed; the quality control method has guidance significance for clinical medication.

The invention discloses a method for determining the content of multi-component chemical components in Chinese pulsatilla root decoction. The method specifically comprises the following steps: respectively establishing standard curves of anemosides B4, B5, D and A3, hederasaponin A1, alpha-hederasaponin, aesculin, aesculetin, fraxetin, phellodendrine, palmatine hydrochloride, coptisine hydrochloride and berberine hydrochloride by utilizing an HPLC-MSMS (High Performance Liquid Chromatography-Mass Spectrometry Mass Spectrometry) analysis method; measuring a Chinese pulsatilla root decoction sample extracted by a decoction method, making a standard curve, and measuring the content of the 13 chemical components. A scientific basis is provided for quality control of the Chinese pulsatilla root decoction and related preparations thereof.

The invention discloses a preparation method of palmatine hydrochloride. By adopting a method for synthesizing palmatine hydrochloride from berberine hydrochloride, damage to plant resources caused byextraction of the palmatine hydrochloride from plants and the environmental pollution caused by the use of a large amount of sulfuric acid in an extraction process can be avoided, and the ecologicalenvironment is protected. The preparation method has the advantages of adoption of cheap and readily-available reaction raw materials, avoidance of the use of highly-toxic conventional methylation reagent dimethyl sulfate or iodomethane or bromomethane, short synthetic route, easiness in operation and suitability for industrial production.

The invention belongs to the technical field of veterinary drug detection, and particularly relates to a UPLC-PDA and QAMS combined detection method for Wujin granules. According to the detection method, an ultra-high performance liquid chromatography-photodiode array detection method is adopted, a spectrogram is used for identification, and a chromatogram is used for quantitative content detection. A quantitative analysis of multiple components by single marker is established, honokiol is used as an internal reference substance, relative correction factors between honokiol and palmatine hydrochloride, berberine hydrochloride and magnolol are established, and content calculation is carried out by using the obtained relative correction factors, so that quantitative analysis of multiple components by single marker is realized. According to the method, the four substances can be quickly qualitatively and quantitatively detected, the method is high in accuracy and reproducibility, purchaseand investment of reference substances are reduced, and funds are saved.

The invention discloses a total alkaloid of Stephania delavayi Diels, the content of which is measured by tetrahydropalmatine. Tetrahydropalmatine occupies 55-95 percent of the total alkaloid extract of Stephania delavayi Diels, wherein the weight percentage of tetrahydropalmatine contained is no less than 2 percent, while the weight percentage of palmatine hydrochloride contained is no less than0.8 percent. The invention also discloses a preparation method of the total alkaloid of Stephania delavayi Diels, which relates to the steps as follows: adding the Stephania delavayi Diels meal into ethanol for diacolation or reflux to acquire alcohol extract, decompressing and recycling the ethanol and concentrating into a condensed ointment for dissolving and filtrating with acid to acquire a filtrate, passing the filtrate through a macroporous resin column or a strong acid cation exchange resin column for adsorption, washing with water to remove the impurities and extracting with an eluent, collecting the eluent and decompressing and recycling the extract and then concentrating and drying, thus acquiring the total alkaloid of Stephania delavayi Diels; the steps can also be decompressing a percolating liquid to recycle the ethanol, concentrating, dissolving with acid and then filtrating, adjusting the pH value of the filtrate with weak base to produce deposition, filtrating the deposition to acquire a filter residue, adding ethanol into the filter residue to resolve and decompress, recycle the ethanol and then drying, thus acquiring the total alkaloid. The results of a pharmacological study on the acquired total alkaloid show that the total alkaloid of Stephania delavayi Diels has good effect on spasmolysis, pain relieving, antiphlogosis and antibiosis, blood activating and so on, and can combine with the acceptable components in pharmacy to prepare various sustained release or controlled release formulation or other formulations.

The traditional Chinese medicine composition of the present invention adopts the UPLC method to simultaneously measure the contents of the active ingredients calycosin glucoside, privetin, palmatine hydrochloride, berberine hydrochloride and rosmarinic acid. The method is simple and fast, and is applicable to this invention. The quality control of the inventive traditional Chinese medicine composition provides a reference for the dynamic and constant monitoring in the continuous production of the inventive traditional Chinese medicine composition.

The invention provides a medicine composition for preventing and / or treating alcoholic stomach injury. The medicine composition comprises berberine hydrochloride, palmatine hydrochloride, evodiamine and rutaecarpin. The invention provides an application of a drug combination of berberine hydrochloride, palmatine hydrochloride, evodiamine and rutaecarpin in a drug for preventing and / or treating alcoholic stomach injury. Research finds that the pharmaceutical composition can significantly improve pathological changes of stomach tissues in an acute alcohol stomach injury rat model, can significantly improve functional indexes (improving superoxide dismutase (SOD) activity and reducing malondialdehyde (MDA) and gastrin (GAS) content) in serum of acute alcohol injury modeling rats, can reduce indexes of inflammatory factors (IL-1, iNOS and TNF-alpha), and can significantly reduce inflammatory factors (IL-1, iNOS and TNF-alpha) in serum of the acute alcohol injury modeling rats. The gene expression and the protein expression of inflammatory factors (IL-1, IL-6 and TNF-alpha) in stomach tissues are reduced, and a good effect of relieving the alcoholic stomach injury is achieved.

The invention discloses a pharmaceutical formulation for treating gastric ulcer and a preparation method of the pharmaceutical formulation. The pharmaceutical formulation comprises main materials of 4 parts of radix tinosporae and 1 part of Chinese olive, and comprises agents of ethanol, methanol, a standard substance of palmatine hydrochloride, and a standard substance of gallic acid. The preparation method comprises: extraction of active ingredients of radix tinosporae and Chinese olive, measurement of the content of the active ingredients of radix tinosporae and Chinese olive, preparation of a solution of a reference substance, exploration of a linear relation, and preparation of a test solution. In comparison with a medicine for treating related diseases in the prior art, the pharmaceutical formulation is relatively pure, is accurate in curative effect and short in course of treatment, and has no toxic or side effect. In comparison with the prior art, the preparation method takes 70% of ethanol as an extraction solvent, has no toxic effect on the body, is low in cost, and is suitable for industrial mass production. The main anti-ulcer component of palmatine hydrochloride in radix tinosporae is subjected to optimization of an extraction process and quantitative analysis through high performance liquid chromatography. The pharmaceutical formulation and the preparation method provide a theoretical reference for promoting development and utilization of radix tinosporae, and have promotion and application value.