Establishment method for variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules and standard fingerprint spectrum thereof

A standard fingerprint and Pulsatilla decoction technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of Psyllium decoction particle fingerprint research, etc., to achieve product quality monitoring, good reproducibility, and high precision Effect

Inactive Publication Date: 2013-11-13
DIHON PHARMA GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the quality control of Pulsatilla Decoction in China is limited to the content determination of the main components, and there is no literature report on the research on the fingerprint of Pulsatilla Decoction particles

Method used

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  • Establishment method for variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules and standard fingerprint spectrum thereof
  • Establishment method for variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules and standard fingerprint spectrum thereof
  • Establishment method for variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules and standard fingerprint spectrum thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Detection of Variable Wavelength Fingerprint of Pulsatilla Decoction Granules

[0041] 1 Instruments and reagents

[0042] 1.1 Instruments: Agilent 1260 liquid chromatograph (Agilent 1260 quaternary pump, MWD detector, DAD detector, Agilent 1260 autosampler), Agilent Chemstation chromatography workstation;

[0043] 1.2 Reagents:

[0044] Jatrorrhizine hydrochloride, palmatine hydrochloride, berberine hydrochloride, berberine hydrochloride, berberine hydrochloride, quincetin, plescetine B4, Pulsatilla saponin B4 standard (National Institute for the Identification of Pharmaceutical and Biological Products, batch numbers are: 0733-200005, 110732-200506 , 110713-200606, 120913-200407, 110740-200104, 110741-200506, 111766-200601); acetonitrile and methanol are chromatographically pure, water is double distilled water, and other reagents are analytically pure.

[0045] 2. High performance liquid chromatography

[0046] 2.1 Chromatographic conditions: the chromat...

Embodiment 2

[0053] Example 2: Detect the standard fingerprints of 10 batches of Pulsatillae Decoction Granules

[0054] Get 10 batches of Pulsatilla Decoction Granules (respectively denoted as BTWT-1~BTWT-10), detect according to the conditions of Example 1, and obtain the HPLC collection of samples of 10 batches, as figure 1 As shown, by comparing 10 batches of HPLC chromatograms, the similarity evaluation is carried out to determine its characteristic common peak:

[0055] There are 25 characteristic common peaks in the fingerprint, now the retention time of the spectrum, the relative retention time of the peak area, the relative peak area, and the percentage of the total peak area are summarized, as shown in Table 1:

[0056] Table 1: Relative retention time, relative peak area and percentage of total peak area of ​​25 peaks

[0057]

[0058]

[0059] In the fingerprints, the variable wavelength fingerprints of Pulsatilla Decoction granules contain 25 characteristic common peaks...

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Abstract

The invention discloses an establishment method for a variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules. The method comprises the following steps of (1) preparing reference substance solutions: preparing reference substance solutions of berberine hydrochloride, palmatine hydrochloride, coptisine, jatrorrhizine hydrochloride, aesculin, aesculetin and pulchinenoside anemoside B4 respectively; (2) preparing a test sample solution: weighing the Chinese pulsatilla root decoction granules, extracting the Chinese pulsatilla root decoction granules and filtering an extracted solution by using a microporous membrane to obtain the test sample solution; (3) obtaining the fingerprint spectrum by determining with high performance liquid chromatography, wherein chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler by a chromatographic column; gradient elution is adopted; variable wavelengths are detected by ultraviolet light wavelengths; and (4) evaluating similarity: evaluating the fingerprint spectrum of the test sample by "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese medicine (Edition 2004A)". The method is simple, stable, high in precision and good in repeatability, and can identify the authenticity and merits of products accurately.

Description

technical field [0001] The invention relates to a quality control method of pulsatilla decoction, in particular to a method for establishing variable-wavelength fingerprints of pulsatilla soup particles and a standard fingerprint. Background technique [0002] Pulsatilla Decoction is derived from "Treatise on Febrile Diseases". Its prescription is Pulsatilla, Cortex Phellodendron, Coptidis Rhizoma, and Qinpi four herbs, which have the functions of clearing away heat and detoxification, cooling blood and stopping dysentery (Xie Ming. Fangji Xue. Beijing: People's Health Publishing House, 2002: 134). It is traditionally used for symptoms such as dysentery, tenesmus, purulent blood in the stool, thirst for drinking water, burning anus, red tongue with yellow coating, and rapid pulse caused by heat and toxin deep in the blood. This prescription is a representative prescription for treating damp-heat dysentery. At present, it is mainly used clinically to treat digestive system ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36
Inventor 杨义芳黄春跃唐博雅罗泽渊李存勇朱绪民蒲琴
Owner DIHON PHARMA GROUP
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