Establishment method for variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules and standard fingerprint spectrum thereof
A standard fingerprint and Pulsatilla decoction technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of Psyllium decoction particle fingerprint research, etc., to achieve product quality monitoring, good reproducibility, and high precision Effect
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Embodiment 1
[0040] Example 1: Detection of Variable Wavelength Fingerprint of Pulsatilla Decoction Granules
[0041] 1 Instruments and reagents
[0042] 1.1 Instruments: Agilent 1260 liquid chromatograph (Agilent 1260 quaternary pump, MWD detector, DAD detector, Agilent 1260 autosampler), Agilent Chemstation chromatography workstation;
[0043] 1.2 Reagents:
[0044] Jatrorrhizine hydrochloride, palmatine hydrochloride, berberine hydrochloride, berberine hydrochloride, berberine hydrochloride, quincetin, plescetine B4, Pulsatilla saponin B4 standard (National Institute for the Identification of Pharmaceutical and Biological Products, batch numbers are: 0733-200005, 110732-200506 , 110713-200606, 120913-200407, 110740-200104, 110741-200506, 111766-200601); acetonitrile and methanol are chromatographically pure, water is double distilled water, and other reagents are analytically pure.
[0045] 2. High performance liquid chromatography
[0046] 2.1 Chromatographic conditions: the chromat...
Embodiment 2
[0053] Example 2: Detect the standard fingerprints of 10 batches of Pulsatillae Decoction Granules
[0054] Get 10 batches of Pulsatilla Decoction Granules (respectively denoted as BTWT-1~BTWT-10), detect according to the conditions of Example 1, and obtain the HPLC collection of samples of 10 batches, as figure 1 As shown, by comparing 10 batches of HPLC chromatograms, the similarity evaluation is carried out to determine its characteristic common peak:
[0055] There are 25 characteristic common peaks in the fingerprint, now the retention time of the spectrum, the relative retention time of the peak area, the relative peak area, and the percentage of the total peak area are summarized, as shown in Table 1:
[0056] Table 1: Relative retention time, relative peak area and percentage of total peak area of 25 peaks
[0057]
[0058]
[0059] In the fingerprints, the variable wavelength fingerprints of Pulsatilla Decoction granules contain 25 characteristic common peaks...
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