67results about How to "Chromatographic conditions are easy" patented technology

The invention provides a method for establishing an HPLC fingerprint spectrum of Radix Glycyrrhizae and a standard fingerprint spectrum thereof. The method comprises the following steps of: firstly adding Radix Glycyrrhizae powder into a 70% ethanol aqueous solution, ultrasound-extracting, filtering the supernate with a microporous membrane to obtain a filtrate as the test solution; then carrying out HPLC analysis, wherein the chromatographic column is Kromasil-C18 (5 mum, 250*4.6 mm), the mobile phase is 0.8% acetic acid solution-acetonitrile, the gradual eluting mode is adopted, the detection wavelength is 254 nm and the column temperature is 30 DEG C; and extracting the test solution, injecting into the HPLC to obtain the HPLC fingerprint spectrum of Radix Glycyrrhizae. The standard fingerprint spectrum of Radix Glycyrrhizae can be obtained by comparing HPLC fingerprint spectra of 16 batches of Radix Glycyrrhizae materials and determining the common fingerprint characteristics. The standard fingerprint spectrum can be used for effectively monitoring the quality of Radix Glycyrrhizae.

The invention provides a method for establishing an HPLC fingerprint spectrum of Fructus Lycii and a standard fingerprint spectrum thereof. The method comprises the following steps of: preparing a test solution, preparing a reference solution, selecting chromatographic conditions, and carrying out HPLC determination to obtain the fingerprint spectrum of Fructus Lycii. The standard fingerprint spectrum of Fructus Lycii can be obtained by comparing HPLC fingerprint spectra of 21 batches of Fructus Lycii materials and determining the common fingerprint characteristics. The method has the advantages of simple operation, good reproducibility, a plurality of characteristic peaks, accurate and reliable result, etc. The standard fingerprint spectrum can be used for effectively monitoring the quality of Fructus Lycii.

The invention discloses a quality control method of liquorice formula granules, belonging to the technical field of analysis of traditional Chinese medicines. The method is used for identifying the liquorice formula granules by adopting a high performance liquid characteristic chromatography, and comprises the following steps: (a) preparing a reference substance solution; (b) preparing a test solution; (c) detecting by adopting the high performance liquid chromatography to obtain the characteristic chromatogram of the liquorice formula granules. According to the established high performance liquid characteristic chromatogram of the liquorice formula granules, five characteristic peaks can be obtained, and as shown in the figure, the peak marked by No. 5 is a reference peak, and the retention time is taken as the following measured values: 8.066 minutes (peak 1), 8.943 minutes (peak 2), 17.708 minutes (peak 3), 21.245 minutes (peak 4) and 30.618 minutes (peak 5) (S). Every gram of liquorice formula granules is equivalent to 10-20g of crude drug. The method has the characteristics of being high in specificity and accuracy; the detection method is scientific and advanced and has operability. The characteristic chromatogram technology is applied to the quality control of the traditional Chinese medicine formula granules, so that the product quality is effectively controlled.

The invention provides a construction method of ultrahigh performance liquid chromatography (UPLC) fingerprint of medicinal raw ginger, and belongs to the technical field of pharmaceutical analysis. The method is characterized by comprising the following steps: (1) precisely weighing about 1.25 grams of raw ginger, placing the raw ginger into a volumetric flask with a volume of 10 mL, adding methanol until the methanol reaches the scale, subjecting the volumetric flask to a supersonic treatment for 25 minutes, cooling, weighing, adding methanol to compensate the weight loss, shaking, filtering with a filter membrane with a pore size of 0.22 [mu]m, saving the filtrate for later use; (2) fingerprint preparation: adopting an ultrahigh performance liquid chromatography (UPLC) system, wherein the chromatographic column is a UPLCBEHC18 chromatographic column (2.1mm*100mm, 1.7[mu]m); acetonitrile (A)-water solution (B) is the mobile phase, gradient elution is adopted, the detection wavelength is 280 nm, the flow speed is 0.35 mL.min<-1>, and the column temperature is 35 DEG C, carrying out analysis so as to obtain UPLC fingerprint of medicinal raw ginger; (3) determination of standard fingerprint: establishing UPLC fingerprints of 17 batches of medicinal raw gingers from different production areas, and determining 16 common characteristic peaks, wherein the fingerprint characteristic of medicinal raw ginger is composed of the 16 common characteristic peaks, and can be used as the standard fingerprint of medicinal raw ginger.

The invention discloses a Chinese angelica standard decoction fingerprint spectrum, a characteristic spectrum establishment method and a content determination method. The establishment method for thefingerprint spectrum comprises the following steps of 1) preparing a test solution; 2) carrying out chromatographic condition and system suitability test; and 3) injecting the test solution into a liquid chromatograph, and measuring to obtain a fingerprint spectrum. An ultra-high performance liquid chromatography fingerprint spectrum of the Chinese angelica standard decoction is researched, so that a better Chinese angelica standard decoction fingerprint spectrum is obtained, and a novel technical method is provided for controlling the quality of the preparation.

The invention provides a method for establishing a coltsfoot flower medicine HPLC (High Performance Liquid Chromatography) fingerprint and a standard fingerprint thereof. The method comprises the following steps of: taking coltsfoot flower medicine powder, adding ethanol, carrying out ultrasonic extraction, filtering supernate by using a micro-pore filter film, and taking the filtrate as a sample solution; then, carrying out high-performance liquid chromatography, wherein a chromatographic column is a Diamonsil-C18 column (5 mum, 250*4.6 mm); taking 0.1% of phosphoric acid-acetonitrile as a mobile phase, and adopting a gradient elution way; detecting the wavelength of 240 nm and the column temperature of 30 DEG C; introducing the sample by absorbing the sample solution to obtain the coltsfoot flower medicine HPLC fingerprint; and comparing 10 batches of coltsfoot flower medicine HPLC fingerprints and determining common fingerprint characteristics to obtain the standard fingerprint. The quality of coltsfoot flower medicine can be effectively monitored by utilizing the standard fingerprint, so that stability, uniformity and controllability of the coltsfoot flower medicine can be kept.

The invention relates to a traditional Chinese medicine (TCM) fingerprint establishment method, in particular to a radix salvia miltiorrhiza high-performance liquid chromatograph (HPLC) fingerprint establishment method and a radix salvia miltiorrhiza standard fingerprint obtained by adopting the method, provides a TCM fingerprint establishment method and a standard fingerprint, and aims to solve the problem that TCM substance identification and composition analysis can not effectively reflect and control the total body mass of TCM at present. The method comprises the following steps: firstly, preparation of a test solution; and secondly, high-performance liquid chromatograph analysis. The radix salvia miltiorrhiza fingerprint can be obtained by adopting the method. The method is used in the technical field of medicine analysis.

The invention discloses an establishment method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of an allinase inactivated extract of six-peal red garlics. The establishment method comprises the following specific steps of preparing six-peal red bulbs which belong to a peculiar variety in Baodi County into a test product solution by a solid-phase extraction step and the like; performing HPLC detection under certain conditions to obtain the HPLC fingerprint spectrum of the allinase inactivated extract of the six-peal red garlics; analyzing the HPLC fingerprint spectrums of ten batches of samples to determine a common characteristic peak and obtaining a standard fingerprint spectrum which can provide a reliable basis for the identification of varieties and production places of the six-peal red garlics and control over the production quality. The establishment method disclosed by the invention has the advantages of convenience in operation, high stability, high specificity, good reproducibility, more characteristic peaks and high analytic efficiency; by using the establishment method disclosed by the invention, the authenticity and production place of the six-peal red garlics can be identified, and the internal quality of the six-peal red garlics can be evaluated comprehensively, accurately and effectively.

The invention belongs to the field of analytical chemistry, and particularly relates to a method for determining related substances in alanyl-glutamine or a preparation thereof, and the method adopts HPLC (High Performance Liquid Chromatography) for detection. Wherein a chromatographic column takes octadecyl acylamino bonded silica gel as a filling agent and takes a sodium octanesulfonate solution-acetonitrile (95: 5) as a mobile phase. The method can effectively separate nine related substances of the alanyl-glutamine, is good in specificity and high in sensitivity, and can realize accurate and quantitative determination of the alanyl-glutamine or a preparation of the alanyl-glutamine.

The invention provides a construction method of a rheum lhasaense fingerprint spectrum and a standard fingerprint spectrum of rheum lhasaense. High performance liquid chromatograph is conducted on a rheum lhasaense sample through a chromatographic column taking octadecylsilane chemically bonded silica as packing, the fingerprint spectrum is constructed by taking piceatannol 3'-O-glucoside as a reference peak, and the fingerprint spectrum is evaluated on this basis. The obtained rheum lhasaense fingerprint spectrum can comprehensively, systematically and characteristically reflect composition ingredients in rheum lhasaense, comprehensively and effectively evaluate the quality of rheum lhasaense and objectively reflect authenticity and merits of a product and is beneficial for product quality monitoring. The method is easy and convenient to operate, stable, high in precision and good in reproducibility.

The invention provides a method for constructing the UPLC fingerprint of a Callicarpa nudiflora medicinal material, and a standard fingerprint of the Callicarpa nudiflora medicinal material, obtainedthrough the method. The method for constructing the UPLC fingerprint of the Callicarpa nudiflora medicinal material comprises the following steps: preparing a sample solution, and carrying out ultra performance liquid chromatography. The method has the characteristics of simplicity, easiness in realization of chromatography conditions, high precision, and goo stability and good reproducibility ofan analysis result; and compared with other test analysis methods in the prior art, the method in the invention has the advantages of shortening of the online analysis time and saving of the materialcost and the time cost.

The invention provides a method for constructing the UPLC fingerprint of Medicinal Rhizome and the standard fingerprint of Medicinal Rhododendron obtained by the method. The method for constructing the UPLC fingerprint of Medicinal Rhizome according to the present invention includes the steps of preparing the test sample solution and the step of ultra-high performance liquid chromatography analysis. According to the method of the present invention, the method is simple, the chromatographic analysis conditions are easy to realize, the precision is high, and the stability and reproducibility of the analysis results are better. Compared with other test and analysis methods in the prior art, the online analysis time is shortened and the Material cost and time cost.

The invention relates to an analysis technology of pharmaceutical intermediates, in particular to a method for analyzing the purity of N-p-aminobenzoyl-L-glutamic acid through chromatography. No method for analyzing the purity of an N-p-aminobenzoyl-L-glutamic acid sample exits in the prior art so that the purity and the quality of the N-p-aminobenzoyl-L-glutamic acid sample can not be analyzed and monitored, thus the quality of a folic acid synthesized by using the N-p-aminobenzoyl-L-glutamic acid as a raw material can not be ensured, and medical effect can not be ensured. In the method of the invention, the purity of the N-p-aminobenzoyl-L-glutamic acid sample is analyzed with an internal standard curve method by adopting a common C18 column matching with an ultraviolet detector, taking an H3PO4-KH2PO4 buffer solution-methanol system as a mobile phase and adopting a sulfanilic acid as an internal standard substance, wherein the flow velocity of the mobile phase is set as 0.3 milliliter/minute. The method has the advantages of easily realized analysis conditions, high stability of the sample, good repeatability of an analysis result and high accuracy of the analysis result.

The invention discloses a method for constructing a fingerprint of Yangweishu granules. The method comprises the steps of: extracting the Yangweishu granules as atest solution, determining at least 10batches of the Yangweishu granules according to an optimized chromatographic condition to obtain a corresponding chromatogram, and introducing the chromatogram into the similarity evaluation system of the traditional Chinese medicine chromatographic fingerprint prescribed by the Chinese Pharmacopoeia Commission to obtain the fingerprint of the Yangweishu granules. The method for constructing thefingerprint of the Yangweishu granuleshas the characteristics of convenience and simplicity in method, good stability, high precision and good reproducibility, can fully reflect material information of a traditional Chinese medicine preparation, and provides a new analytical method for quality inspection and quality control of the Yangweishu Granules.

The invention provides an establishment method of a scorzonera fingerprint spectrum. The establishment method comprises the following specific steps: preparing a test solution by scorzonera, and carrying out fractionation detection under a certain condition by a high performance liquid chromatograph to obtain the scorzonera fingerprint spectrum. The invention further provides a scorzonera standard fingerprint spectrum obtained by the method. The standard fingerprint spectrum can provide reliable basis for scorzonera identification and internal mass control. The establishment method is simple and convenient to operate, high in stability, good in reproducibility and multiple in characteristic peaks. By utilizing the establishment method, the quality of scorzonera medicinal materials can be comprehensively and accurately evaluated.

The invention discloses a method for establishing an HPLC fingerprint of a dichloromethane part of the root of costus speciosus (koening) smith as a Yao medicinal material. The method comprises the following steps: (1), preparing a test solution; (2), preparing a reference solution by using diosgenin as a standard product; (3), carrying out HPLC detection; to be specific, recording an HPLC map within 95 minutes; and (4), detecting a plurality of test products, carrying out matching of all chromatographic peaks by using "Traditional Chinese medicine chromatographic fingerprint similarity evaluation system" software and a multi-point calibration method, and generating a reference fingerprint R automatically as a reference fingerprint. According to the invention, with establishment of the fingerprint of the root of costus speciosus (koening) smith, the relative relationship of the components contained in the root of costus speciosus (koening) smith can be reflected comprehensively; the medicinal materials from different origin places can be distinguished effectively; and the quality can be evaluated and controlled well. Therefore, the development and utilization of the root of costus speciosus (koening) smith in Guangxi province can be promoted.

The invention discloses an establishment method of inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints specifically comprises the steps of preparing inducing callus cultured Allium sativum L. bulbs into solution for test products through steps such as solid-phase extraction, and performing high-performance chromatographic analysis and detection under certain conditions to obtain HPLC fingerprints of inducing callus cultured Allium sativum L. enzymatic hydrolysate. By analyzing the HPLC fingerprints of 10 batches of samples, characteristic common peaks are determined and standard fingerprints are obtained. The standard fingerprints can provide a reliable basis for variety identification and production quality control of inducing callus cultured Allium sativum L. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints has the advantages that the operation is convenient, the stability is high, the specificity is strong, the repeatability is good, the characteristic peaks are more, the analysis efficiency is high, the truth identification of the inducing callus cultured Allium sativum L. can be realized by adopting the invention, and the interior product quality can be comprehensively, accurately and effectively evaluated.

The invention relates to the field of the quality control technology of traditional Chinese medicinal materials and in particular discloses an establishment method of an Aquilaria sinensis leaf high performance liquid chromatography (HPLC) fingerprint spectrum. The establishment method comprises the steps of preparing reference solution, preparing test article solution, determining the chromatographic conditions of the fingerprint spectrum, and determining a standard fingerprint spectrum; the establishment method is characterized in that the chromatographic conditions of the fingerprint spectrum are as follows: a C18 chromatographic column is adopted, acetonitrile-0.1-0.5% of phosphate buffer solution is used as mobile phase for gradient elution; an ultraviolet detector is used for detection and the detection wavelength is 300-360nm. The fingerprint spectrum has strong characteristic feature, main common peaks can achieve good baseline separation, a test article can be prepared simply and conveniently, the chromatographic conditions can be realized easily, and the method is simple and convenient, has good stability and good reproducibility and is suitable for the quality control of Aquilaria sinensis leaves.

The invention discloses a lumbar kidney cream fingerprint spectrum establishing method and a lumbar kidney reference fingerprint spectrum. The fingerprint spectrum establishing method comprises the steps that (1) a sample is pretreated, wherein volatile components in lumbar kidney cream are absorbed by ethyl acetate to serve as a test sample for gas phase identification, water is added into the remaining cream, and the mixture is used as a test sample for liquid phase identification after being subjected to methanol ultrasonic extraction and liquid immersion; (2) gas phase analysis is performed on the test sample for gas phase identification to obtain a lumbar kidney cream gas phase fingerprint spectrum; and (3) liquid phase analysis is performed on the test sample for liquid phase identification to obtain a lumbar kidney cream liquid phase fingerprint spectrum. Through research about the lumbar kidney cream fingerprint spectra, a good lumbar kidney cream fingerprint spectrum is obtained, and a new technical method is provided for controlling the quality of the preparation.

The invention discloses a method for establishing a quantitative fingerprint spectrum of a fructus evodiae standard decoction, and relates to the field of establishment of fingerprint spectrums.The method comprises the following steps that 1, a reference substance solution is prepared; (2) preparing a test solution; (3) carrying out ultra-high performance liquid chromatography analysis; and (4) establishing a quantitative fingerprint, introducing multiple batches of liquid chromatograms into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, matching common peaks, generating a control characteristic spectrum, selecting reference peaks, calculating the relative retention time of each characteristic peak, and determining the content of three components, namely limonin, evodiamine and rutaecarpin. And establishing a quantitative fingerprint spectrum of the fructus evodiae standard decoction. According to the method, the characteristic chromatogram and the content of the fructus evodiae standard decoction can be detected at the same time through one-time analysis, the detection time can be effectively shortened, the working efficiency is improved, and a guarantee is provided for quality and consistency evaluation of fructus evodiae formula granules.