73results about How to "Comprehensive identification" patented technology

The invention discloses a transcriptome-based tumor antigen identification method. The method comprises four steps of: obtaining an RNA sample of a patient tumor tissue, and carrying out library construction and amplification on the RNA sample to obtain an RNA sample sequencing result of the tumor tissue; aligning short read segments of the RNA sample sequencing result to a human reference genometo obtain an RNA alignment result; calculating gene expression quantity according to the RNA alignment result, and carrying out mutation detection and prediction of fusion gene events according to theRNA alignment result; and predicting transcriptome HLA typing according to the alignment result, wherein calculation of the gene expression quantity, mutation detection and prediction of the fusion gene events are carried out according to a specified order or simultaneously carried out; and using the gene expression quantity of a transcriptome sample, depth of transcriptome mutation sites in a whole-exon sequencing sample and binding force of neonatal short peptides and the patient HLA typing as an analysis result to submit the same to a downstream analyst. The invention provides the method capable of identifying a tumor-specific antigen of an individual sample from tumor patient transcriptome NGS data.

The invention discloses a cypress microsatellite molecular marker combination, and a primer combination and screening method and application thereof, and belongs to the technical field of forest molecular biology. In the application, a method for identifying the polymorphism of cypress chloroplast microsatellite molecular marker primers comprises the steps that an M13 tail sequence is added at the 5' terminal of a forward primer of a designed primer pair, then the designed primer pair is mixed with a specific reverse primer, a general M13 primer with a fluorescence marker and a genome DNA, PCR amplification and electrophoretic separation sequencing are carried out, and polymorphic primers of cypress microsatellite molecular markers are obtained. A method for identifying the genetic relationship of cypress comprises the steps that the genome DNA of cypress is extracted, PCR amplification and electrophoretic separation are carried out with the polymorphic primers of the cypress microsatellite molecular markers, capillary electrophoresis sequencing is carried out, STR polymorphism is counted, the polymorphic primers can reflect the genetic information of cypress plasmon and can be used for researches such as genetic resource classification, genetic diversity analysis and the like.

The invention provides a fluorogenic culture medium of Enterobacter sakazakii, a test kit and a test method thereof, and relates to a method for testing microbe and a composition used by the method. The fluorogenic culture medium comprises the compositions as follows: 8 to 20 grams of peptone, 4 to 7 grams of beef extract powder, 4 to 7 grams of sodium chloride, 1.0 to 2.0 grams of cholate, 12 to 20 grams of agar, 0.05 to 0.5 grams of X-a-glucopyranoside, 0.01 to 0.04 grams of Na2CO3, 0.3 to 2.0 grams of ferric ammonium citrate, and 0.3 to 2.0 grams of sodium thiosulfate. The test kit consists of the fluorogenic culture medium of Enterobacter sakazakii, an enriched liquid A ,namely buffer peptone water culture medium, and an enriched liquid B, namely modified lauryl sulfate pancreas peptone broth. The test kit is configured simply, and the test method has high sensitivity in detection, so the method is suitable for treating large flux samples.

A gene chip for detecting the pathogenic bacteria from blood features that the specific probes, derivative probes, complementary probes, and universal probes are arranged on the nylon film to detect different pathogenic bacteria. Its advantages are high speed and high correctness.

The invention discloses a machine learning technology-based intelligent seal impression recognition and anti-fake method. The method comprises the following steps of: obtaining original seal impression data and data acquired during sealing by an intelligent impression anti-fake database; carrying out image acquisition on a to-be-detected impression in a limited area and carrying out preprocessingto obtain a naked impression; carrying out matching verification on naked impression data comprising seal specification information and the original seal impression data to obtain a weight value of the seal specification information; carrying out matching verification on feature data of a to-be-detected impression content view and the data acquired during the sealing so as to obtain a weight valueof the impression content view; carrying out matching verification on feature data of a to-be-detected background content view and the data acquired during the sealing so as to obtain a weight valueof the background content view; and carrying out weight add operation on the obtained weight value of the seal specification information, weight value of the impression content view and weight value of the background content view, and judging whether the impression is true or not through a total weight value so as to achieve the aim of rapidly verifying whether the seal is true or not.

The invention discloses a building method of an HPLC characteristic chromatogram of radix paeoniae alba and a detection method of radix paeoniae alba. For a quality control problem of radix paeoniae alba, a sulfur-containing radix paeoniae alba HPLC characteristic chromatogram and a sulfur-free radix paeoniae alba HPLC characteristic chromatogram are built, and a high performance liquid chromatography-mass spectrometry analytic method is adopted to analyze and identify ingredients of sulfur-containing radix paeoniae alba and sulfur-free radix paeoniae alba. Whether radix paeoniae alba is fumigated with sulfur or not is judged by adopting the built HPLC characteristic chromatogram of radix paeoniae alba to identify a paeoniflorin sulfite product which is produced after dry sulphitation of radix paeoniae alba. Compared with a conventional sulfur dioxide detection method, the detection method provided by the invention is simple, high in sensitivity, strong in specificity and excellent inreproducibility, can be used for detecting whether radix paeoniae alba is fumigated with sulfur or not, therefore, can identify the authenticity and quality of radix paeoniae alba more comprehensively, accurately and quickly, and is particularly suitable for detecting and identifying large-scale production of radix paeoniae alba.

The invention discloses cowpea chloroplast SSR molecule labeled polymorphic primers and a screening method thereof and a method for identifying genetic relationship of cowpeas, belonging to the technical field of molecular biology of crops. The cowpea chloroplast SSR molecule labeled polymorphic primers are described in the specification. The method for identifying polymorphism of the cowpea chloroplast SSR molecule labeled primers comprises the following steps: extracting genomic DNAs of cowpeas of different varieties; mixing designed primers and the genomic DNAs and carrying out PCR amplification and electrophoretic separation; and acquiring the cowpea chloroplast SSR molecule labeled polymorphic primers. The method for identifying the genetic relationship of cowpeas comprises the following steps: extracting genomic DNA of a cowpea; carrying out PCR amplification and electrophoretic separation by using the cowpea chloroplast SSR molecule labeled polymorphic primers and recording statistical results; and carrying out genetic relationship analysis on the results. The polymorphic primers can reflect genetic information of plasmon of cowpeas and can be used for research on germplasm resource classification, genetic relationship identification, genetic diversity analysis and the like of cowpeas.

The invention relates to a method for rapidly analyzing a key aroma compound of yellow rice wine. The method comprises the steps of: (1), extracting an aroma compound in yellow rice wine; (2), performing qualitative and quantitative analysis on the aroma compound in the yellow rice wine by using a gas chromatography and mass spectrometry combination technology; (3), by utilizing a sensory evaluation method, performing descriptive analysis on the yellow rice wine by selecting eight sensory attributes; (4), inspecting the correlation relations between the aroma compound and the eight sensory attributes by utilizing a partial least square method, and determining a characteristic aroma component in the yellow rice wine according to the correlation between the aroma component and the sensory attribute; (5), analyzing an aroma active compound in the yellow rice wine by utilizing a gas chromatography and olfactory combination technology; (6), recombining the aroma of the yellow rice wine by utilizing an aroma model established by the key aroma compound; and (7), further verifying and determining the key aroma compound of the yellow rice wine by utilizing an omission experiment. The methodin the invention is simple to operate and intuitional and reliable in result, and can be used for identifying aroma compounds in different kinds of yellow rice wine; and thus, the applicability is wide.

The invention discloses a quality control method of liquorice formula granules, belonging to the technical field of analysis of traditional Chinese medicines. The method is used for identifying the liquorice formula granules by adopting a high performance liquid characteristic chromatography, and comprises the following steps: (a) preparing a reference substance solution; (b) preparing a test solution; (c) detecting by adopting the high performance liquid chromatography to obtain the characteristic chromatogram of the liquorice formula granules. According to the established high performance liquid characteristic chromatogram of the liquorice formula granules, five characteristic peaks can be obtained, and as shown in the figure, the peak marked by No. 5 is a reference peak, and the retention time is taken as the following measured values: 8.066 minutes (peak 1), 8.943 minutes (peak 2), 17.708 minutes (peak 3), 21.245 minutes (peak 4) and 30.618 minutes (peak 5) (S). Every gram of liquorice formula granules is equivalent to 10-20g of crude drug. The method has the characteristics of being high in specificity and accuracy; the detection method is scientific and advanced and has operability. The characteristic chromatogram technology is applied to the quality control of the traditional Chinese medicine formula granules, so that the product quality is effectively controlled.

The invention provides a cell subset annotation method based on single cell transcriptome sequencing. The cell subset annotation method comprises the following steps: 1) 10x barcode UMI identification; 2) genome comparison; 3) gene expression profile comparison; 4) low-quality cell filtering and data homogenization; 5) cell population clustering; 6) marker gene extraction; 7) cell subset annotation. The invention belongs to the technical field of biological information analysis, and provides a cell subset annotation method based on single-cell transcriptome sequencing, which solves the problem of single-cell subset annotation, so that single-cell sequencing data can support cell annotation according to a gene expression profile and / or a cell Marker gene after conventional analysis. Therefore, organic combination of different annotation methods is realized, and the distribution condition and related information of cell types are obtained.

The present invention relates to color developing culture medium, detecting kit and detection method for Vibrio parahaemolyticus, and belongs to the field of food microbe safety monitoring technology. The detecting kit consists of color developing culture medium comprising bacterial specific enzyme in 0.05-0.95 g and mixed color developing substrate Y-glucoside, enrichment liquid A of pancreatic casitone liquid culture medium and enrichment liquid B comprising sodium chloride, polymyxin and broth. The detection method includes the enrichment culture of the sample in enrichment liquid A and enrichment liquid B, the culture of the two parts of enrichment cultured liquid in the color developing culture medium, and judging whether to exist Vibrio parahaemolyticus according to whether to have smooth raised purple colony of 2-3 mm diameter. The present invention has low cost, high detection sensitivity, short detection period and other advantages.

The invention discloses a method for distinguishing edible oil from swill-cooked dirty oil by electrochemical fingerprints. The method comprises the following steps of: respectively taking different varieties of edible oil, swill-cooked dirty oil and samples to be detected; respectively adding the edible oil, the swill-cooked dirty oil and the samples to be detected into a B-Z oscillation system with a constant temperature, and adding surfactant into the B-Z oscillation system, thus obtaining the electrochemical fingerprints of the edible oil, the swill-cooked dirty oil and the samples to be detected with taking a platinum electrode as an indication electrode and a double-salt-bridge saturated calomel electrode or Ag / AgCl electrode as a reference electrode; and extracting characteristic data from the electrochemical fingerprints, and mixing the characteristic data by a mode identifying manner and then carrying out classification on the edible oil, the swill-cooked dirty oil and the samples to be detected. The method provided by the invention solves the difficult problems that the edible oil is not dissolved in water, is poor in reaction repeatability and disturbs fingerprint detection; the samples do not need to be treated, and are directly fed for overall detection; and the method has the advantages of simple operation, low cost and rapid analysis, and can be used for distinguishing the edible oil from the swill-cooked dirty oil objectively, accurately and reliably by sufficiently utilizing various fingerprint characteristics of the edible oil, thus laying the foundation for comprehensively controlling the quality and the security of the edible oil.

The invention provides a magnetic image sensor and an authentic identification method. The magnetic image sensor comprises a magnetic sensor chip. The characteristics are that: the magnetic sensor chip comprises a first magnetic sensitive dot matrix and a second magnetic sensitive unit dot matrix; an included angle theta between the first magnetic sensitive dot matrix and the second magnetic sensitive unit dot matrix is in the range of 0<theta<180 degrees; the first magnetic sensitive dot matrix and the second magnetic sensitive unit dot matrix respectively comprise N magnetic sensitive units and M magnetic sensitive units; and the magnetic sensitive units are Wheatstone bridge circuits made of magnetic sensitive films, wherein the N and the M are integers greater or equal to 1. The magnetic image sensor can carry out magnetic imaging on magnetic stripes or magnetic dots in a two dimensional plane, so that demands for two dimension authentic identification can be realized.

The invention provides an SNP molecular marker and method for identifying mycobacteria, a primer composition, a kit and application, and relates to the technical field of strain identification. The SNP molecular marker comprises seventeen SNP loca distributed in an Hsp65 gene, an rrs gene and a pncA gene, and when marked in mycobacterium tuberculosis, mycobacterium bovis, Africa mycobacterium, mycobacterium avium, intracellular mycobacterium, mycobacterium fortuitum, mycobacterium kansasii, mycobacterium gastri, mycobacterium marinum,volemycobacterium, mycobacterium scrofulaceum, mycobacteriumsmegmatis, suga mycobacterium, mycobacterium abscessus, mycobacterium ulcerans, toad mycobacterium, mycobacterium gordonae and ape mycobacterium, the SNP molecular marker has polymorphism. The primercomposition can amplify the Hsp65 gene, therrs gene and thepncA gene to be used for analyzing of the SNP molecular marker and strain identification.

The invention discloses a method for identifying chuanxiong rhizomes in far-reaching tonic wine. The method is a thin layer chromatography method that chuanxiong rhizome reference medicine materials and European angelica lactone A reference products are adopted as reference, and the ratio of normal hexane to ethyl acetate is (3-6):(1-2). The method for identifying the chuanxiong rhizomes in the far-reaching tonic wine is easy to operate and high in controllability, and the chuanxiong rhizome ingredient in the far-reaching tonic wine can be efficiently identified. The method is high in separation degree, good in reproducibility, high in specificity, high in sensitivity and good in stability, feminine reference is free of interference, and thin layer plates of different factories and the temperature and humidity conditions all have no influence. According to the method for identifying chuanxiong rhizomes in the far-reaching tonic wine, the chuanxiong rhizome ingredient in the far-reaching tonic wine can be identified accurately, comprehensively, scientifically and effectively, the quality detection standard is improved, and the stability, safety and effectiveness of product quality are further ensured.

The invention relates to the field of biotechnology, in particular to an SNP marker as well as application and a detection method thereof. A great amount of individuals of Chinese Apis mellifera are subjected to whole genome sequencing, so that 21 specific SNP messages in the Chinese Apis mellifera are obtained and the 21 specific SNP messages serve as marker SNP sites for identifying the Chinese Apis mellifera. The special SNP sites in the Chinese Apis mellifera provided by the invention play an important role in the differentiation of the Chinese Apis mellifera with the Apis mellifera in other regions as well as in the adaptive evolution of the Chinese Apis mellifera to local environment.

The invention belongs to the technical field of Chinese patent medicaments, and relates to a quality control method for a Chinese medicinal preparation, namely heat clearing and blood cooling pills prepared from Chinese medicinal materials. The heat clearing and blood cooling pills consist of Chinese medicinal materials, namely scutellaria root and Rehmannia glutinosa. The quality control method comprises the following steps of: taking baicalin and catalpol as reference products, and identifying whether scutellaria root and Rehmannia glutinosa components are contained in the prescription of the heat clearing and blood cooling pills by thin-layer chromatography; and detecting the content of the baicalin in the prescription of the heat clearing and blood cooling pills by high performance liquid chromatography. A quantitative index and an inspection method thereof make the quality standard more perfect. The revised quality standard improves the quality control of medicaments.

This invention relates to a multiple PCR quick test reagent box of food- pathogens often seen in water including 10xPCR reaction buffer solution, MgCl2, dNTP, a primer and Hot star Taq enzymes. The test method includes: picking up due test sample templates, adding 10xPCR buffer solution, MgCl2, dNTP, primers, the Hot star Taq enzymes, the due test sample and water in a PCR thin-wall tube to be mixed and put on the PCR instrument to be enlarged in an electrophoresis device, finally the result is analyzed and judged.

The invention discloses a primer for rapidly identifying mycobacterium tuberculosis complex strains and an application of the primer. The primer for rapidly identifying the mycobacterium tuberculosis complex strains consists of a primer pair 1, a primer pair 2, a primer pair 3, a primer pair 4, a primer pair 5, a primer pair 6 and a primer pair 7. The experiment shows that the primer can be used for accurately identifying the mycobacterium tuberculosis complex strains, the 7 pairs of the primers are under one same amplification reaction condition, amplification of 7 different primers is completed at one time, multiple times of amplification is not needed, the operation is simplified, and the time can be saved as the operation is rapid to implement.

The invention relates to medical in-vitro diagnostic techniques, in particular to a reverse dot-blot hybridization gene chip. A plurality of microarrays in different areas are distributed on a nylon membrane, and a common specific probe for postoperative intracranial infection pathogenic bacteria is fixed in each microarray area. The reverse dot-blot hybridization gene chip can be used for identifying enterococcocci (enterococcus faecium and enterococcus faecalis), staphylococcus aureus, coagulase-negative staphylococci, escherichia coli, Klebsiella pneumonia, pseudomonas aeruginosa, acinetobacter baumannii and enterobacter cloacae. The reverse dot-blot hybridization gene chip further comprises a universal Gram-negative bacterium probe and a universal Gram-positive bacterium probe. The chip can be used for simultaneously detecting whether various pathogenic bacteria exist or not, has the advantages of rapidity and accuracy in diagnosis, high specificity and heavy information, and is of great help to clinical diagnosis and epidemic disease screening.

The invention provides a method for identifying specific sequence fragments of key enzyme genes in specific plant species. According to the method, a phylogenetic relationship of key enzyme genes in different plant groups is reconstructed by using analysis methods of molecular systematics and bioinformatics, sequences, structures and differentiation among the different groups are compared and analyzed, and specific sequence fragments and key variation sites of the enzyme in specific plant species are identified. The invention also provides application of the obtained specific sequence fragmentin plant or specific medicinal material source identification, variety improvement or breeding.

The invention relates to a chromogenic medium for identification of escherichia coli. The chromogenic medium contains one chromogenic substrate of 4-nitrophenyl-beta-D-glucopyranoside, o-nitrophenol beta-D glucuronide, 4-nitrophenyl-beta-D-glucopyranoside, 4-methylumbelliferyl-beta-D-glucuronide and 5-bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide, and the chromogenic substrate interacts with glucosaccharase of the escherichia coli to release chromogenic groups. With the adoption of the chromogenic medium for identification of the escherichia coli and the application of the chromogenicmedium, the components are simple, the operation is convenient, detection sensitivity and specificity are high, false positive and false negative are reduced, furthermore, the detection cycle is short, the chromogenic medium is applicable to treatment of large batches of samples, industrialized production is facilitated, and the escherichia coli in food can be detected comprehensively, symmetrically and accurately and identified primarily.

The invention provides an early fire monitoring and early warning method and device, and the method comprises the steps: 1, collecting the image information of an indoor environment through a camera disposed indoors so as to carry out the judgment of a fire; monitoring the indoor environment parameter information capable of representing the fire disaster in real time through a sensor installed in an indoor monitoring area where the fire breaks out easily; 2, processing the indoor environment image information and the environment parameter information to respectively obtain a flame detection posterior probability and a fire hazard posterior probability; and 3, inputting the flame detection posterior probability and the fire hazard posterior probability as basic reliability functions of different evidence sources into a DS evidence theory for decision fusion so as to obtain a fire hazard prediction result. According to the invention, a multi-characteristic quantity data fusion method is utilized, the fire prediction model is created, the accuracy and the stability are improved, early warning of the fire can be achieved, the fire disaster can be timely and effectively monitored, and the possibility of fire occurrence is reduced to the minimum.

The invention relates to a power transmission line fault reason intelligent identification method based on a relevance vector machine, and belongs to the field of power fault analysis. The method comprises the steps of determining a power transmission line fault cause type, constructing a fault sample set based on historical fault recording data of a power transmission line, performing resampling to obtain a capacity expansion sample set, obtaining a sample intrinsic dimension, dividing the capacity expansion sample set into subsets, obtaining a feature subset and a new sample set, and dividing a training sample set and a test sample set. And a fault reason identification module is constructed and trained, and the fault reason identification module identifies a single fault with an unknown fault reason. The method disclosed by the invention can effectively extract and screen typical fault features of the power transmission line, overcomes the problem that the number of samples of each fault reason type is unbalanced, can provide an intelligent identification conclusion for the fault reasons of the power transmission line and the accuracy of the conclusion for engineering technicians, and has higher fault reason identification accuracy compared with other prior art.

The invention discloses an identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor. The method is a thin-layer chromatography method by using a kirenol reference substance as control and using trichloromethane-methanol as a developing solvent, wherein the ratio of trichloromethane to methanol is 3-5: 1-2. By the identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor, herba siegesbeckiae in anti-rheumatism medicinal liquor can be efficiently and accurately identified. The method has higher controllability, is simple to operate, has high separation degree and good reappearance, has no interference with negative control, and will not be influenced by thin layer plates from different manufacturers and temperature and humidity conditions. According to the identification method, herba siegesbeckiae component in anti-rheumatism medicinal liquor can be identified accurately, comprehensively, scientifically and effectively. Quality detection standard of the anti-rheumatism medicinal liquor is raised, and stability, safety and effectiveness of product quality are further ensured.

The invention belongs to the technical field of biology and particularly relates to a tumor antigen expression examination primer and kit based on a high-throughput sequencing method and application of the tumor antigen expression examination primer and kit. The kit comprises plural pairs of amplification primers of a plurality of tumor antigens of Chinese common tumors (including lung cancer, liver cancer, pancreatic cancer, colorectum cancer and the like), and a reagent for RNA reverse transcription required in high-throughput sequencing, library construction and sequencing. The primer and kit can be used for performing once examination and analysis on a sample, can complete RNA expression level examination of 58, and has the characteristics of high throughput, high sensitivity, low examination list, high specificity and the kit.

The invention provides an automatic capture system. The automatic capture system comprises a vehicle detection module, a Gigabit network camera, a DSP digital image processing module and an optical fiber transceiver module, thereby having sensitive vehicle detection, high shooting pixels, more identification content and high transmission efficiency to be capable of providing top-quality data for police officers; a red light detection module is arranged by aiming at the application in a transportation hub, shooting, storage, processing and transmission are started when a red light is on to save a lot of software and hardware resources; a lighting supplementing module is arranged to assist the Gigabit network camera to obtain top-quality panoramic images.

The invention belongs to the technical field of biological engineering, in particular to a construction method and application of a mycobacterium tuberculosis gene expression library, and the invention is characterized in that two expression carriers pETEXba and pGEXMCM that are applicable to the construction of the mycobacterium tuberculosis gene expression library are prepared, all encoding genes of mycobacterium bacteria are obtained through PCR reaction and precisely cloned into the constructed expression carriers, and finally the mycobacterium tuberculosis gene expression library is obtained by transforming a colon bacillus expression strain BL21(DE3). The invention realizes preliminary application of the library and proves that the library can effectively filter new protection antigens. Nucleotide sequences of the constructed expression carriers pETEXba and pGEXMCM are described in SEQ ID NO.1 and SEQ ID NO.2. The method of the invention has low cost, high flux and high coverage rate, and can be used in the filtration of new protection antigens and medicine targets.

The invention discloses an identification method for ligusticum wallichii in ostealgia medicinal liquor. The identification method is a thin layer chromatography method, in the thin layer chromatography method, a ligusticum wallichii contrast medicinal material is used as contrast, and normal hexane and ethyl acetate by the ratio of (8-12):(1-2) are used as developing solvent. According to the identification method for ligusticum wallichii in the ostealgia medicinal liquor, ligusticum wallichii in the ostealgia medicinal liquor can be efficiently and accurately identified, the identification method is easy to operate, high in controllability, high in separation degree and sensitivity, good in precision, stability and repeatability, negative contrast is not interfered, and the identification method is not affected by thin layer plates from different manufacturers or temperature or humidity conditions. The identification method can accurately, comprehensively, scientifically and effectively identify the ligusticum wallichii component in ostealgia medicinal liquor, promotes quality detection standards and further ensures stable product quality, safety and effectiveness.