73results about How to "Comprehensive identification" patented technology

The invention discloses a transcriptome-based tumor antigen identification method. The method comprises four steps of: obtaining an RNA sample of a patient tumor tissue, and carrying out library construction and amplification on the RNA sample to obtain an RNA sample sequencing result of the tumor tissue; aligning short read segments of the RNA sample sequencing result to a human reference genometo obtain an RNA alignment result; calculating gene expression quantity according to the RNA alignment result, and carrying out mutation detection and prediction of fusion gene events according to theRNA alignment result; and predicting transcriptome HLA typing according to the alignment result, wherein calculation of the gene expression quantity, mutation detection and prediction of the fusion gene events are carried out according to a specified order or simultaneously carried out; and using the gene expression quantity of a transcriptome sample, depth of transcriptome mutation sites in a whole-exon sequencing sample and binding force of neonatal short peptides and the patient HLA typing as an analysis result to submit the same to a downstream analyst. The invention provides the method capable of identifying a tumor-specific antigen of an individual sample from tumor patient transcriptome NGS data.

The invention provides a video fake traffic discriminating method, device and electronic device. The video fake traffic discriminating method comprises the steps of obtaining the video page watching frequency and video page clicking amount within a preset period; making the video page clicking amount and the video page watching frequency as the quotient, and obtaining the page clicking rate corresponding to the video page; obtaining a playing end source of a playing video page; discriminating the playing of the video page as the fake traffic playing when the page clicking rate reaches to the first preset condition corresponding to the page clicking rate and the playing end source reaches to the second preset condition corresponding to the playing end source. The video fake traffic discriminating method, device and electronic device can discriminate video fake traffic in a more comprehensive manner.

The invention discloses a cypress microsatellite molecular marker combination, and a primer combination and screening method and application thereof, and belongs to the technical field of forest molecular biology. In the application, a method for identifying the polymorphism of cypress chloroplast microsatellite molecular marker primers comprises the steps that an M13 tail sequence is added at the 5' terminal of a forward primer of a designed primer pair, then the designed primer pair is mixed with a specific reverse primer, a general M13 primer with a fluorescence marker and a genome DNA, PCR amplification and electrophoretic separation sequencing are carried out, and polymorphic primers of cypress microsatellite molecular markers are obtained. A method for identifying the genetic relationship of cypress comprises the steps that the genome DNA of cypress is extracted, PCR amplification and electrophoretic separation are carried out with the polymorphic primers of the cypress microsatellite molecular markers, capillary electrophoresis sequencing is carried out, STR polymorphism is counted, the polymorphic primers can reflect the genetic information of cypress plasmon and can be used for researches such as genetic resource classification, genetic diversity analysis and the like.

The invention provides a fluorogenic culture medium of Enterobacter sakazakii, a test kit and a test method thereof, and relates to a method for testing microbe and a composition used by the method. The fluorogenic culture medium comprises the compositions as follows: 8 to 20 grams of peptone, 4 to 7 grams of beef extract powder, 4 to 7 grams of sodium chloride, 1.0 to 2.0 grams of cholate, 12 to 20 grams of agar, 0.05 to 0.5 grams of X-a-glucopyranoside, 0.01 to 0.04 grams of Na2CO3, 0.3 to 2.0 grams of ferric ammonium citrate, and 0.3 to 2.0 grams of sodium thiosulfate. The test kit consists of the fluorogenic culture medium of Enterobacter sakazakii, an enriched liquid A ,namely buffer peptone water culture medium, and an enriched liquid B, namely modified lauryl sulfate pancreas peptone broth. The test kit is configured simply, and the test method has high sensitivity in detection, so the method is suitable for treating large flux samples.

A gene chip for detecting the pathogenic bacteria from blood features that the specific probes, derivative probes, complementary probes, and universal probes are arranged on the nylon film to detect different pathogenic bacteria. Its advantages are high speed and high correctness.

The invention discloses a machine learning technology-based intelligent seal impression recognition and anti-fake method. The method comprises the following steps of: obtaining original seal impression data and data acquired during sealing by an intelligent impression anti-fake database; carrying out image acquisition on a to-be-detected impression in a limited area and carrying out preprocessingto obtain a naked impression; carrying out matching verification on naked impression data comprising seal specification information and the original seal impression data to obtain a weight value of the seal specification information; carrying out matching verification on feature data of a to-be-detected impression content view and the data acquired during the sealing so as to obtain a weight valueof the impression content view; carrying out matching verification on feature data of a to-be-detected background content view and the data acquired during the sealing so as to obtain a weight valueof the background content view; and carrying out weight add operation on the obtained weight value of the seal specification information, weight value of the impression content view and weight value of the background content view, and judging whether the impression is true or not through a total weight value so as to achieve the aim of rapidly verifying whether the seal is true or not.

The invention discloses a quality control method of liquorice formula granules, belonging to the technical field of analysis of traditional Chinese medicines. The method is used for identifying the liquorice formula granules by adopting a high performance liquid characteristic chromatography, and comprises the following steps: (a) preparing a reference substance solution; (b) preparing a test solution; (c) detecting by adopting the high performance liquid chromatography to obtain the characteristic chromatogram of the liquorice formula granules. According to the established high performance liquid characteristic chromatogram of the liquorice formula granules, five characteristic peaks can be obtained, and as shown in the figure, the peak marked by No. 5 is a reference peak, and the retention time is taken as the following measured values: 8.066 minutes (peak 1), 8.943 minutes (peak 2), 17.708 minutes (peak 3), 21.245 minutes (peak 4) and 30.618 minutes (peak 5) (S). Every gram of liquorice formula granules is equivalent to 10-20g of crude drug. The method has the characteristics of being high in specificity and accuracy; the detection method is scientific and advanced and has operability. The characteristic chromatogram technology is applied to the quality control of the traditional Chinese medicine formula granules, so that the product quality is effectively controlled.

The present invention relates to color developing culture medium, detecting kit and detection method for Vibrio parahaemolyticus, and belongs to the field of food microbe safety monitoring technology. The detecting kit consists of color developing culture medium comprising bacterial specific enzyme in 0.05-0.95 g and mixed color developing substrate Y-glucoside, enrichment liquid A of pancreatic casitone liquid culture medium and enrichment liquid B comprising sodium chloride, polymyxin and broth. The detection method includes the enrichment culture of the sample in enrichment liquid A and enrichment liquid B, the culture of the two parts of enrichment cultured liquid in the color developing culture medium, and judging whether to exist Vibrio parahaemolyticus according to whether to have smooth raised purple colony of 2-3 mm diameter. The present invention has low cost, high detection sensitivity, short detection period and other advantages.

The invention discloses a method for distinguishing edible oil from swill-cooked dirty oil by electrochemical fingerprints. The method comprises the following steps of: respectively taking different varieties of edible oil, swill-cooked dirty oil and samples to be detected; respectively adding the edible oil, the swill-cooked dirty oil and the samples to be detected into a B-Z oscillation system with a constant temperature, and adding surfactant into the B-Z oscillation system, thus obtaining the electrochemical fingerprints of the edible oil, the swill-cooked dirty oil and the samples to be detected with taking a platinum electrode as an indication electrode and a double-salt-bridge saturated calomel electrode or Ag/AgCl electrode as a reference electrode; and extracting characteristic data from the electrochemical fingerprints, and mixing the characteristic data by a mode identifying manner and then carrying out classification on the edible oil, the swill-cooked dirty oil and the samples to be detected. The method provided by the invention solves the difficult problems that the edible oil is not dissolved in water, is poor in reaction repeatability and disturbs fingerprint detection; the samples do not need to be treated, and are directly fed for overall detection; and the method has the advantages of simple operation, low cost and rapid analysis, and can be used for distinguishing the edible oil from the swill-cooked dirty oil objectively, accurately and reliably by sufficiently utilizing various fingerprint characteristics of the edible oil, thus laying the foundation for comprehensively controlling the quality and the security of the edible oil.

The invention provides a magnetic image sensor and an authentic identification method. The magnetic image sensor comprises a magnetic sensor chip. The characteristics are that: the magnetic sensor chip comprises a first magnetic sensitive dot matrix and a second magnetic sensitive unit dot matrix; an included angle theta between the first magnetic sensitive dot matrix and the second magnetic sensitive unit dot matrix is in the range of 0<theta<180 degrees; the first magnetic sensitive dot matrix and the second magnetic sensitive unit dot matrix respectively comprise N magnetic sensitive units and M magnetic sensitive units; and the magnetic sensitive units are Wheatstone bridge circuits made of magnetic sensitive films, wherein the N and the M are integers greater or equal to 1. The magnetic image sensor can carry out magnetic imaging on magnetic stripes or magnetic dots in a two dimensional plane, so that demands for two dimension authentic identification can be realized.

The invention provides an SNP molecular marker and method for identifying mycobacteria, a primer composition, a kit and application, and relates to the technical field of strain identification. The SNP molecular marker comprises seventeen SNP loca distributed in an Hsp65 gene, an rrs gene and a pncA gene, and when marked in mycobacterium tuberculosis, mycobacterium bovis, Africa mycobacterium, mycobacterium avium, intracellular mycobacterium, mycobacterium fortuitum, mycobacterium kansasii, mycobacterium gastri, mycobacterium marinum,volemycobacterium, mycobacterium scrofulaceum, mycobacteriumsmegmatis, suga mycobacterium, mycobacterium abscessus, mycobacterium ulcerans, toad mycobacterium, mycobacterium gordonae and ape mycobacterium, the SNP molecular marker has polymorphism. The primercomposition can amplify the Hsp65 gene, therrs gene and thepncA gene to be used for analyzing of the SNP molecular marker and strain identification.

The invention discloses a method for identifying chuanxiong rhizomes in far-reaching tonic wine. The method is a thin layer chromatography method that chuanxiong rhizome reference medicine materials and European angelica lactone A reference products are adopted as reference, and the ratio of normal hexane to ethyl acetate is (3-6):(1-2). The method for identifying the chuanxiong rhizomes in the far-reaching tonic wine is easy to operate and high in controllability, and the chuanxiong rhizome ingredient in the far-reaching tonic wine can be efficiently identified. The method is high in separation degree, good in reproducibility, high in specificity, high in sensitivity and good in stability, feminine reference is free of interference, and thin layer plates of different factories and the temperature and humidity conditions all have no influence. According to the method for identifying chuanxiong rhizomes in the far-reaching tonic wine, the chuanxiong rhizome ingredient in the far-reaching tonic wine can be identified accurately, comprehensively, scientifically and effectively, the quality detection standard is improved, and the stability, safety and effectiveness of product quality are further ensured.

This invention relates to a multiple PCR quick test reagent box of food- pathogens often seen in water including 10xPCR reaction buffer solution, MgCl2, dNTP, a primer and Hot star Taq enzymes. The test method includes: picking up due test sample templates, adding 10xPCR buffer solution, MgCl2, dNTP, primers, the Hot star Taq enzymes, the due test sample and water in a PCR thin-wall tube to be mixed and put on the PCR instrument to be enlarged in an electrophoresis device, finally the result is analyzed and judged.

The invention discloses a primer for rapidly identifying mycobacterium tuberculosis complex strains and an application of the primer. The primer for rapidly identifying the mycobacterium tuberculosis complex strains consists of a primer pair 1, a primer pair 2, a primer pair 3, a primer pair 4, a primer pair 5, a primer pair 6 and a primer pair 7. The experiment shows that the primer can be used for accurately identifying the mycobacterium tuberculosis complex strains, the 7 pairs of the primers are under one same amplification reaction condition, amplification of 7 different primers is completed at one time, multiple times of amplification is not needed, the operation is simplified, and the time can be saved as the operation is rapid to implement.

The invention provides a method for identifying specific sequence fragments of key enzyme genes in specific plant species. According to the method, a phylogenetic relationship of key enzyme genes in different plant groups is reconstructed by using analysis methods of molecular systematics and bioinformatics, sequences, structures and differentiation among the different groups are compared and analyzed, and specific sequence fragments and key variation sites of the enzyme in specific plant species are identified. The invention also provides application of the obtained specific sequence fragmentin plant or specific medicinal material source identification, variety improvement or breeding.

The invention relates to a chromogenic medium for identification of escherichia coli. The chromogenic medium contains one chromogenic substrate of 4-nitrophenyl-beta-D-glucopyranoside, o-nitrophenol beta-D glucuronide, 4-nitrophenyl-beta-D-glucopyranoside, 4-methylumbelliferyl-beta-D-glucuronide and 5-bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide, and the chromogenic substrate interacts with glucosaccharase of the escherichia coli to release chromogenic groups. With the adoption of the chromogenic medium for identification of the escherichia coli and the application of the chromogenicmedium, the components are simple, the operation is convenient, detection sensitivity and specificity are high, false positive and false negative are reduced, furthermore, the detection cycle is short, the chromogenic medium is applicable to treatment of large batches of samples, industrialized production is facilitated, and the escherichia coli in food can be detected comprehensively, symmetrically and accurately and identified primarily.

The invention provides an early fire monitoring and early warning method and device, and the method comprises the steps: 1, collecting the image information of an indoor environment through a camera disposed indoors so as to carry out the judgment of a fire; monitoring the indoor environment parameter information capable of representing the fire disaster in real time through a sensor installed in an indoor monitoring area where the fire breaks out easily; 2, processing the indoor environment image information and the environment parameter information to respectively obtain a flame detection posterior probability and a fire hazard posterior probability; and 3, inputting the flame detection posterior probability and the fire hazard posterior probability as basic reliability functions of different evidence sources into a DS evidence theory for decision fusion so as to obtain a fire hazard prediction result. According to the invention, a multi-characteristic quantity data fusion method is utilized, the fire prediction model is created, the accuracy and the stability are improved, early warning of the fire can be achieved, the fire disaster can be timely and effectively monitored, and the possibility of fire occurrence is reduced to the minimum.

The invention relates to a power transmission line fault reason intelligent identification method based on a relevance vector machine, and belongs to the field of power fault analysis. The method comprises the steps of determining a power transmission line fault cause type, constructing a fault sample set based on historical fault recording data of a power transmission line, performing resampling to obtain a capacity expansion sample set, obtaining a sample intrinsic dimension, dividing the capacity expansion sample set into subsets, obtaining a feature subset and a new sample set, and dividing a training sample set and a test sample set. And a fault reason identification module is constructed and trained, and the fault reason identification module identifies a single fault with an unknown fault reason. The method disclosed by the invention can effectively extract and screen typical fault features of the power transmission line, overcomes the problem that the number of samples of each fault reason type is unbalanced, can provide an intelligent identification conclusion for the fault reasons of the power transmission line and the accuracy of the conclusion for engineering technicians, and has higher fault reason identification accuracy compared with other prior art.

The invention discloses an identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor. The method is a thin-layer chromatography method by using a kirenol reference substance as control and using trichloromethane-methanol as a developing solvent, wherein the ratio of trichloromethane to methanol is 3-5: 1-2. By the identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor, herba siegesbeckiae in anti-rheumatism medicinal liquor can be efficiently and accurately identified. The method has higher controllability, is simple to operate, has high separation degree and good reappearance, has no interference with negative control, and will not be influenced by thin layer plates from different manufacturers and temperature and humidity conditions. According to the identification method, herba siegesbeckiae component in anti-rheumatism medicinal liquor can be identified accurately, comprehensively, scientifically and effectively. Quality detection standard of the anti-rheumatism medicinal liquor is raised, and stability, safety and effectiveness of product quality are further ensured.

The invention belongs to the technical field of biology and particularly relates to a tumor antigen expression examination primer and kit based on a high-throughput sequencing method and application of the tumor antigen expression examination primer and kit. The kit comprises plural pairs of amplification primers of a plurality of tumor antigens of Chinese common tumors (including lung cancer, liver cancer, pancreatic cancer, colorectum cancer and the like), and a reagent for RNA reverse transcription required in high-throughput sequencing, library construction and sequencing. The primer and kit can be used for performing once examination and analysis on a sample, can complete RNA expression level examination of 58, and has the characteristics of high throughput, high sensitivity, low examination list, high specificity and the kit.

The invention provides an automatic capture system. The automatic capture system comprises a vehicle detection module, a Gigabit network camera, a DSP digital image processing module and an optical fiber transceiver module, thereby having sensitive vehicle detection, high shooting pixels, more identification content and high transmission efficiency to be capable of providing top-quality data for police officers; a red light detection module is arranged by aiming at the application in a transportation hub, shooting, storage, processing and transmission are started when a red light is on to save a lot of software and hardware resources; a lighting supplementing module is arranged to assist the Gigabit network camera to obtain top-quality panoramic images.

The invention belongs to the technical field of biological engineering, in particular to a construction method and application of a mycobacterium tuberculosis gene expression library, and the invention is characterized in that two expression carriers pETEXba and pGEXMCM that are applicable to the construction of the mycobacterium tuberculosis gene expression library are prepared, all encoding genes of mycobacterium bacteria are obtained through PCR reaction and precisely cloned into the constructed expression carriers, and finally the mycobacterium tuberculosis gene expression library is obtained by transforming a colon bacillus expression strain BL21(DE3). The invention realizes preliminary application of the library and proves that the library can effectively filter new protection antigens. Nucleotide sequences of the constructed expression carriers pETEXba and pGEXMCM are described in SEQ ID NO.1 and SEQ ID NO.2. The method of the invention has low cost, high flux and high coverage rate, and can be used in the filtration of new protection antigens and medicine targets.

The invention discloses an identification method for ligusticum wallichii in ostealgia medicinal liquor. The identification method is a thin layer chromatography method, in the thin layer chromatography method, a ligusticum wallichii contrast medicinal material is used as contrast, and normal hexane and ethyl acetate by the ratio of (8-12):(1-2) are used as developing solvent. According to the identification method for ligusticum wallichii in the ostealgia medicinal liquor, ligusticum wallichii in the ostealgia medicinal liquor can be efficiently and accurately identified, the identification method is easy to operate, high in controllability, high in separation degree and sensitivity, good in precision, stability and repeatability, negative contrast is not interfered, and the identification method is not affected by thin layer plates from different manufacturers or temperature or humidity conditions. The identification method can accurately, comprehensively, scientifically and effectively identify the ligusticum wallichii component in ostealgia medicinal liquor, promotes quality detection standards and further ensures stable product quality, safety and effectiveness.