2results about How to "Comprehensive identification" patented technology

The application discloses a detection method of Baizhu San standard decoction fingerprint combined with liquid chromatography-mass spectrometry, the fingerprint and application thereof, and belongs to the field of traditional Chinese medicine quality standard research and application. Test sample solution and single control sample solution are injected into a high performance liquid chromatograph to obtain respective chromatograms; mass spectrometry analysis is conducted on the test sample solution to obtain mass spectrometry results; common peaks existing in the chromatogram of the test sample solution are taken as common peaks, chemical components in the Baizhu San standard decoction are identified in combination with the chromatogram of the single control sample solution and the mass spectrometry results of the test sample solution, and the Baizhu San standard decoction fingerprint is obtained. The fingerprint is constructed through liquid chromatography-mass spectrometry, 10 intersecting components are identified in combination with the mass spectrometry information of the control sample, so that the quality control of the Baizhu San standard decoction is realized, the method is simple, easy to operate, high in precision, good in stability and reproducibility, and can effectively solve the technical problem that the quality of the Baizhu San cannot be effectively evaluated.

This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The MNP marker site includes at least one of MNP-1 to MNP-19. The primer set includes at least one primer pair from the first to the 19th primer pair. The forward primer of the first primer pair, the reverse primer of the first primer pair, the forward primer of the 19th primer pair, and the reverse primer of the 19th primer pair are shown sequentially as SEQ ID NO: 1 to SEQ ID NO: 38 in the sequence listing. This disclosure provides an MNP marker site, primer set, kit, and application for identifying multiple pathogens in wastewater. The primer set targets 19 MNP marker sites, and each pathogen can be detected by 1 to 3 marker sites. It can identify multiple pathogens in wastewater, providing comprehensive, efficient, and accurate detection, and is suitable for identifying multiple pathogens in wastewater.