The invention belongs to the technical field of
traditional Chinese medicine identification, and particularly relates to a method for determining specific primary ions of traditional Chinese medicinal materials based on high-resolution liquid
chromatography and application. The method for determining the specific first-grade ions of the traditional Chinese medicinal materials based on the high-resolution liquid
chromatography comprises the following steps: performing extraction and ultra-
high performance liquid chromatography-high-
resolution mass spectrometry analysis on different traditional Chinese medicinal materials by using an
alcohol-containing
solvent; the method comprises the following steps: automatically screening special ultra-
high performance liquid chromatography-
high resolution mass spectrometry first-level ions of each traditional Chinese medicinal material from ultra-
high performance liquid chromatography-
high resolution mass spectrometry data of all traditional Chinese medicinal materials, and constructing a special
ion database of each traditional Chinese medicinal material; the specific
ion database of the traditional Chinese medicinal materials can be used for identifying the traditional Chinese medicinal materials and products thereof. According to the method, the
ion specificity is high, the ultra-high performance liquid
chromatography-
high resolution mass spectrum primary ions, obtained through one-time screening, of all traditional Chinese medicinal materials can be applied to all products containing the traditional Chinese medicinal materials, analysis conditions do not need to be developed again, and the method is rapid, simple, more comprehensive in identification, good in durability and high in universality.