Identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor

A technology of Fengshi Ning medicine and Xixuecao, which is applied in the field of identification of Xixuecao, achieves the effect of short extraction time, good extraction effect, and improved quality inspection standards

Active Publication Date: 2017-02-08
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing quality standard of Fengshining medicinal wine does not involve the identification of Silico chinensis, therefore, it is particularly necessary to study a method that is easy to operate and can accurately identify Silico sibiricus in Fengshining medicinal liquor

Method used

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  • Identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor
  • Identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor
  • Identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The method for identifying the Herba chinensis in Fengshining medicinal liquor comprises the following steps:

[0044] (1) Preparation of the test solution: take 40 mL of this product, add 20 mL of ethyl acetate, shake and extract once to obtain ethyl acetate solution, evaporate to dryness, add 1 mL of methanol to the residue to dissolve it, and use it as the test solution;

[0045] (2) Preparation of reference substance solution: take chinonyl alcohol reference substance, add methanol to make a solution containing 0.1 mg chinonyl alcohol reference substance in every 1 mL, as the reference substance solution;

[0046] (3) Identification: According to the TLC test of "Chinese Pharmacopoeia" in 2015, the four general rules 0502, draw 2 μL of the reference substance solution and 8 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, and use chloroform-methanol =3︰1 is the developer, develop, take out, dry, spray with 5% vanillin sulfuric ac...

Embodiment 2

[0048] The method for identifying the Herba chinensis in Fengshining medicinal liquor comprises the following steps:

[0049] (1) Preparation of the test solution: Take 55mL of this product, add 35mL, 30mL, and 20mL of ethyl acetate respectively, shake and extract 3 times, combine the ethyl acetate solution, evaporate to dryness, add 2mL of methanol to the residue to dissolve it, and use it as The test solution;

[0050] (2) Preparation of reference substance solution: take chinonyl alcohol reference substance, add methanol to make a solution containing 0.1 mg chinonyl alcohol reference substance in every 1 mL, as the reference substance solution;

[0051] (3) Identification: According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0502 thin-layer chromatography test, absorb 4 μL of the reference substance solution and 12 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, with chloroform-methanol =5︰2 is the developing agent,...

Embodiment 3

[0053] The method for identifying the Herba chinensis in Fengshining medicinal liquor comprises the following steps:

[0054] (1) Preparation of the test solution: Take 50 mL of this product, add 20 mL and 30 mL of ethyl acetate respectively, shake and extract twice, combine the ethyl acetate solution, evaporate to dryness, add 1 mL of methanol to the residue to dissolve it, and use it as the test solution. product solution;

[0055] (2) Preparation of reference substance solution: take chinonyl alcohol reference substance, add methanol to make a solution containing 0.1 mg chinonyl alcohol reference substance in every 1 mL, as the reference substance solution;

[0056] (3) Identification: According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0502 thin-layer chromatography test, absorb 3 μL of the reference substance solution and 10 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, with chloroform-methanol =4︰1 is the deve...

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Abstract

The invention discloses an identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor. The method is a thin-layer chromatography method by using a kirenol reference substance as control and using trichloromethane-methanol as a developing solvent, wherein the ratio of trichloromethane to methanol is 3-5: 1-2. By the identification method of herba siegesbeckiae in anti-rheumatism medicinal liquor, herba siegesbeckiae in anti-rheumatism medicinal liquor can be efficiently and accurately identified. The method has higher controllability, is simple to operate, has high separation degree and good reappearance, has no interference with negative control, and will not be influenced by thin layer plates from different manufacturers and temperature and humidity conditions. According to the identification method, herba siegesbeckiae component in anti-rheumatism medicinal liquor can be identified accurately, comprehensively, scientifically and effectively. Quality detection standard of the anti-rheumatism medicinal liquor is raised, and stability, safety and effectiveness of product quality are further ensured.

Description

technical field [0001] The invention relates to a method for identifying Sisia chinensis in Fengshining medicinal wine, and specifically belongs to the technical field of traditional Chinese medicine detection. Background technique [0002] Fengshining Medicinal Wine is made from Spatholobus Polygoni Multiflori, Radix Polygoni Multiflori, Radix Scutellariae, Bran Stir-fried Atractylodes Rhizome, Sagevine, Smilax, Geranium, Safflower, Black Snake, Acanthopanax Bark, Guizhi, Silkworm Sand, Radix Radix Grass, Shichangpu, Sophora flavescens, Asarum, Xugufeng, Galangal, Sangzhi, Angelica dahurica, Rehmannia glutinosa, Xanthium and Chuanxiong. Function is expelling wind and activating blood circulation, promoting dampness and dredging collaterals. In the prescription, Caulis Spatholobus is the monarch drug, which has the effects of promoting blood circulation and dredging collaterals, eliminating phlegm and promoting regeneration, and fluent meridians. The minister uses Polygonu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 包雪爱
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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