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Building method of HPLC characteristic chromatogram of radix paeoniae alba and detection method of radix paeoniae alba

A feature map and construction method technology, applied in the field of traditional Chinese medicine identification, can solve the problems of complicated operation and low detection sensitivity, and achieve the effects of high sensitivity, good reproducibility, accurate and rapid identification

Inactive Publication Date: 2018-05-22
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method for determining the sulfur content in Radix Paeoniae Alba is generally the acid-base titration method stipulated in the 2015 edition of the Chinese Pharmacopoeia, which is cumbersome to operate and relatively low in detection sensitivity.

Method used

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  • Building method of HPLC characteristic chromatogram of radix paeoniae alba and detection method of radix paeoniae alba
  • Building method of HPLC characteristic chromatogram of radix paeoniae alba and detection method of radix paeoniae alba
  • Building method of HPLC characteristic chromatogram of radix paeoniae alba and detection method of radix paeoniae alba

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1: A kind of construction method of Radix Paeoniae Alba HPLC characteristic map

[0041] 1. Instruments and reagents

[0042] Waters high performance liquid chromatography (Waters company, Waters e2695), Agilent high performance liquid chromatography (Agilent company, 1260Infinity), one millionth balance (Mettler-Toledo company, ME204E), millionth A level (Mettler-Toledo, XP26);

[0043] Ethanol (AR), methanol (AR), acetonitrile, methanol, and phosphoric acid for the liquid phase are HPLC chromatographic grades, and water is ultrapure water;

[0044] Gallic acid (National Institute for Food and Drug Control, batch number: 110831-201204, purity: 89.9%); catechin (National Institute for Food and Drug Control, batch number: 110877-201604, purity: 99.2%); paeonifloride ( Chengdu Pufeide Biotechnology Co., Ltd., batch number: 151109. Purity: 98%); Paeoniflorin (National Institute for Food and Drug Control, batch number: 110736-201640, purity: 95.2%); 1,2,3,4,6 ...

Embodiment 2

[0160] Embodiment 2: a kind of quality detection method of Radix Paeoniae Alba

[0161] Including the following steps:

[0162] The preparation of need testing solution: get the Radix Paeoniae Alba sample (batch number: G160915) 0.4g, accurately weighed, put in the conical flask with stopper, add dilute ethanol 50ml accurately, weigh, ultrasonic treatment 20-40 minutes, Let it cool, make up the lost weight with dilute ethanol, shake well, filter, and take the filtrate to get it;

[0163] Identification: Accurately draw 10 μl of the test product solution, inject it into a high-performance liquid chromatograph, and measure it to obtain the characteristic spectrum of Radix Paeoniae Alba to be tested. The chromatographic conditions are the same as in "2.1 Chromatographic conditions".

[0164] Compare the characteristic spectrum of Radix Paeoniae Alba to be tested with the control characteristic spectrum of Radix Paeoniae Alba Sulfur and the control characteristic spectrum of Radi...

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Abstract

The invention discloses a building method of an HPLC characteristic chromatogram of radix paeoniae alba and a detection method of radix paeoniae alba. For a quality control problem of radix paeoniae alba, a sulfur-containing radix paeoniae alba HPLC characteristic chromatogram and a sulfur-free radix paeoniae alba HPLC characteristic chromatogram are built, and a high performance liquid chromatography-mass spectrometry analytic method is adopted to analyze and identify ingredients of sulfur-containing radix paeoniae alba and sulfur-free radix paeoniae alba. Whether radix paeoniae alba is fumigated with sulfur or not is judged by adopting the built HPLC characteristic chromatogram of radix paeoniae alba to identify a paeoniflorin sulfite product which is produced after dry sulphitation of radix paeoniae alba. Compared with a conventional sulfur dioxide detection method, the detection method provided by the invention is simple, high in sensitivity, strong in specificity and excellent inreproducibility, can be used for detecting whether radix paeoniae alba is fumigated with sulfur or not, therefore, can identify the authenticity and quality of radix paeoniae alba more comprehensively, accurately and quickly, and is particularly suitable for detecting and identifying large-scale production of radix paeoniae alba.

Description

technical field [0001] The invention belongs to the field of identification of traditional Chinese medicines, and in particular relates to the construction of an HPLC characteristic map of Radix Paeoniae Alba and a detection method thereof. Background technique [0002] Sulfur fumigation of Chinese herbal medicines is one of the traditional processing methods of Chinese herbal medicines in my country. Fumigation with an appropriate amount of sulfur can achieve the purpose of preventing mildew, insects, moisture and prolonging the storage time of medicinal materials. However, a large number of studies have shown that sulfur fumigation usually significantly reduces the active ingredients of Chinese herbal medicines. More importantly, sulfur compounds remaining in Chinese herbal medicine pieces after sulfur fumigation are harmful to human health, and even transform into carcinogenic nitrosamines in the human body. [0003] Radix Paeoniae Alba is a liver-nourishing and blood-ton...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 丁青朱德全刘桂联霍文杰潘礼业
Owner GUANGDONG YIFANG PHARMA
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