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SNP marker as well as application and detection method thereof

A 935827SNP, 211517SNP technology, applied in the field of SNP marker and its application, detection, can solve the problem of low identification accuracy of bee species

Active Publication Date: 2016-04-27
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (2) The accuracy of bee species identification is low;

Method used

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  • SNP marker as well as application and detection method thereof
  • SNP marker as well as application and detection method thereof
  • SNP marker as well as application and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] (1) Collect bee samples and extract DNA. Samples with a DNAOD value of 1.8-2.0 and a content greater than 1.5 micrograms are considered qualified;

[0069] (2) Qualified DNA samples were used to construct a library: the qualified DNA samples were randomly fragmented into 350bp fragments by a Covaris shredder. TruSeqLibraryConstructionKit was used to build the library, and the reagents and consumables recommended in the instructions were strictly used. The DNA fragments undergo steps such as end repair, tailing, sequencing adapters, purification, and PCR amplification to complete the entire library preparation. The constructed library was sequenced by illuminaHiSeq.

[0070] (3) Library inspection: After the library construction is completed, first use Qubit2.0 for preliminary quantification, dilute the library to 1ng / μl, and then use Agilent2100 to detect the insert size of the library. The effective concentration is accurately quantified (the effective concentration ...

Embodiment 2

[0107] Refer to the method in Example 1 for the test method for identifying the black bee species in Western Regions of China and other bee species through the SNP sites described in Example 1. The test results are shown in Table 1:

[0108] Table 1 SNP genotype identification results

[0109]

[0110] Remarks: K stands for GT heterozygote, S stands for CG heterozygote, R stands for AG heterozygote, Y stands for CT heterozygote; a single A or C or G or T stands for homozygote.

[0111] According to the SNP information provided by the present invention and the identification method, it can be seen that if the SNP information of the 1502972 position of NW_003378054.1 includes T, the species to be tested is the black bee of the Western Regions; , the SNP information of the species to be tested is K, which is a GT heterozygote, so it is a black bee from the Western Regions of China, and can be distinguished from other species. Therefore, the detection method provided by the pr...

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Abstract

The invention relates to the field of biotechnology, in particular to an SNP marker as well as application and a detection method thereof. A great amount of individuals of Chinese Apis mellifera are subjected to whole genome sequencing, so that 21 specific SNP messages in the Chinese Apis mellifera are obtained and the 21 specific SNP messages serve as marker SNP sites for identifying the Chinese Apis mellifera. The special SNP sites in the Chinese Apis mellifera provided by the invention play an important role in the differentiation of the Chinese Apis mellifera with the Apis mellifera in other regions as well as in the adaptive evolution of the Chinese Apis mellifera to local environment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to SNP markers and their applications and detection methods. Background technique [0002] my country has a long history of bee breeding and rich bee germplasm resources, but for a long time, only Apis mellifera has been found in my country, and there is no Apis mellifera. The collection ability of Eastern honeybee is slightly inferior to that of Western honeybee. With the introduction of Western honeybee, more and more beekeepers in my country have begun to raise Western honeybee, and the germplasm resources of native bees in my country are threatened. It is of great significance to the germplasm resources of honey bees in our country if we can find the native Western honeybee in our country. Mr. Shi Wei, director of the China Honeybee Germplasm Resources Committee, and the staff of the Apiculture Management Station of Xinjiang Autonomous Region have been engaged in strengthening the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 陈晓石巍陈超刘之光王慧华郭海坤
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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