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Detection method of liquorice formula granules

A technology of formula granule and determination method, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of extraction of raw materials, inconsistent product technology and quality standards, lack of raw material quality information, and different product quality, etc., and achieve effective product quality. Control, maneuverability, and exclusive effects

Inactive Publication Date: 2015-06-10
BEIJING KANGRENTANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional Chinese medicine formula granules are currently in the state of pilot production in China, and the extraction raw materials used in production, product technology and quality standards are not uniform
Different manufacturers use different raw materials and production processes, resulting in different quality of products produced by each manufacturer, inconsistent drug effects, specificity of quality standards is limited by certain experimental conditions and scope, lack of quality information on raw materials, the current quality Standards cannot comprehensively determine the quality of traditional Chinese medicine formula granules

Method used

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  • Detection method of liquorice formula granules
  • Detection method of liquorice formula granules
  • Detection method of liquorice formula granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: quality control method of licorice formula granule

[0032] (1) Preparation of reference solution

[0033] Take appropriate amount of liquiritin reference substance and ammonium glycyrrhizinate reference substance, weigh them accurately, add 70% ethanol to make solutions containing 20 μg of liquiritin and 0.2 mg of ammonium glycyrrhizinate per 1 ml, respectively, to obtain the reference substance solution.

[0034] (2) Preparation of the test solution

[0035] Take 0.1g of licorice formula granule powder, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 100ml of 70% ethanol precisely, seal it tightly, weigh it, treat it with ultrasound for 30 minutes, let it cool, weigh it again, and use 70% Make up the lost weight with ethanol, shake well, filter, and take the filtrate to obtain the test solution.

[0036] The chromatographic conditions determined by high performance liquid chromatography are:

[0037] The chromatographic column is Wate...

Embodiment 2

[0044] Embodiment 2: quality control method of licorice formula granule

[0045] (1) Preparation of reference solution

[0046] Take appropriate amount of liquiritin reference substance and ammonium glycyrrhizinate reference substance, weigh them accurately, add 70% ethanol to make solutions containing 20 μg of liquiritin and 0.2 mg of ammonium glycyrrhizinate per 1 ml, respectively, to obtain the reference substance solution.

[0047] (2) Preparation of the test solution

[0048] Take 0.05g of licorice formula granule powder, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 50ml of 70% ethanol precisely, seal it tightly, weigh it, treat it ultrasonically for 10 minutes, let it cool, weigh it again, and use 70% Make up the lost weight with ethanol, shake well, filter, and get the continued filtrate to obtain the test solution;

[0049] The chromatographic conditions determined by high performance liquid chromatography are:

[0050] The chromatographic column...

Embodiment 3

[0057] Embodiment 3: quality control method of licorice formula granule

[0058] (1) Preparation of reference solution

[0059]Take appropriate amount of liquiritin reference substance and ammonium glycyrrhizinate reference substance, weigh them accurately, add 70% ethanol to make solutions containing 20 μg of liquiritin and 0.2 mg of ammonium glycyrrhizinate per 1 ml, respectively, to obtain the reference substance solution.

[0060] (2) Preparation of the test solution

[0061] Take 0.2g of licorice formula granule powder, weigh it accurately, put it in a stoppered Erlenmeyer flask, add 100ml of 70% ethanol accurately, seal it tightly, weigh it, treat it ultrasonically for 40 minutes, let it cool, weigh it again, and use 70% Make up the lost weight with ethanol, shake well, filter, and take the filtrate to obtain the test solution.

[0062] The chromatographic conditions determined by high performance liquid chromatography are:

[0063] The chromatographic column is Water...

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Abstract

The invention discloses a quality control method of liquorice formula granules, belonging to the technical field of analysis of traditional Chinese medicines. The method is used for identifying the liquorice formula granules by adopting a high performance liquid characteristic chromatography, and comprises the following steps: (a) preparing a reference substance solution; (b) preparing a test solution; (c) detecting by adopting the high performance liquid chromatography to obtain the characteristic chromatogram of the liquorice formula granules. According to the established high performance liquid characteristic chromatogram of the liquorice formula granules, five characteristic peaks can be obtained, and as shown in the figure, the peak marked by No. 5 is a reference peak, and the retention time is taken as the following measured values: 8.066 minutes (peak 1), 8.943 minutes (peak 2), 17.708 minutes (peak 3), 21.245 minutes (peak 4) and 30.618 minutes (peak 5) (S). Every gram of liquorice formula granules is equivalent to 10-20g of crude drug. The method has the characteristics of being high in specificity and accuracy; the detection method is scientific and advanced and has operability. The characteristic chromatogram technology is applied to the quality control of the traditional Chinese medicine formula granules, so that the product quality is effectively controlled.

Description

technical field [0001] The invention relates to a quality control method of traditional Chinese medicine formula granules, in particular to a method for establishing a high performance liquid chromatography characteristic map of licorice formula granules, and a characteristic map of licorice formula granules obtained by the method, belonging to the field of traditional Chinese medicine analysis. Background technique [0002] Traditional Chinese medicine formula granules are prepared by extracting, concentrating, drying, and granulating the Chinese herbal decoction pieces as raw materials. As a new form of decoction pieces, they can replace Chinese herbal decoction pieces for clinical syndrome differentiation and treatment, and can be added or subtracted according to the symptoms. It ensures the efficacy of traditional Chinese medicine decoction pieces. Compared with the traditional Chinese medicine decoction, it is convenient to take, the dosage is small, the quality is contr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 孙娜李蕊
Owner BEIJING KANGRENTANG PHARMA
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