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Chromogenic medium for identification of escherichia coli and application of chromogenic medium

A chromogenic medium and Escherichia coli technology, which is applied in the field of detection and identification, can solve the problems of detection of Escherichia coli contamination in unfavorable food, reduce the detection rate of Escherichia coli, complicated operation, etc., and achieve a short detection period and easy operation. The effect of industrialized production and high detection sensitivity

Inactive Publication Date: 2019-03-08
SHANGHAI JIKONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the Escherichia coli chromogenic media that are generally recognized and widely used in the market are foreign products, such as French Chromagar (CHRO), bio-Mérieux and other brands. These products have stable quality but are expensive; some major domestic In recent years, it has begun to focus on the independent research and development of chromogenic medium; Chinese patent CN104278075A discloses a chromogenic medium for Escherichia coli, which is based on mTSB broth agar medium with inhibitors and The color agent reduces the cost, but in this invention, the color developer is not explicitly stated, and there are many components, and the operation is relatively cumbersome. At the same time, the novobiocin in the basic medium mTSB used has an inhibitory effect on some target bacteria. The detection rate of Escherichia coli is reduced, and there may be missed detection in the detection of large quantities of food samples, which is not conducive to the detection of Escherichia coli contamination in food

Method used

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  • Chromogenic medium for identification of escherichia coli and application of chromogenic medium
  • Chromogenic medium for identification of escherichia coli and application of chromogenic medium
  • Chromogenic medium for identification of escherichia coli and application of chromogenic medium

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Escherichia coli chromogenic medium components include: agar 15g / L, peptone 5g / L, lactose 11.4g / L, sodium chloride 3g / L, bile salt 2g / L, 5-bromo-4-chloro-3 -Indoxyl-N-acetyl-β-D-glucosamine 0.15g / L, purified water 1000mL.

[0021] Prepare Escherichia coli chromogenic medium as follows:

[0022] (1) Weighing of each component: Weigh agar 15g / L, peptone 5g / L, lactose 11.4g / L, sodium chloride 3g / L, bile salt 2g / L, 5-bromo-4-chloro-3- Indoxyl-N-acetyl-β-D-glucosamine 0.15g / L, placed in a clean and dry container;

[0023] (2) Mixing of raw materials: add 1000mL of purified water to the container in step (1), heat and boil until completely dissolved;

[0024] (3) adjust the pH to be 7.2 ± 0.2;

[0025] (4) Autoclaving: the mixture is subjected to autoclaving (115° C., 15 minutes);

[0026] (5) Dispensing: After sterilization, cool to 50°C under aseptic conditions, pour into sterile plates;

[0027] (6) The prepared plate was pre-incubated at 36±1°C for 24 hours to observ...

Embodiment 2

[0029] Escherichia coli chromogenic medium components include: agar 12g / L, peptone 15g / L, lactose 5 / L, sodium chloride 5g / L, bile salt 1g / L, 4-methylumbelliferyl-β -D-glucuronide 0.1g / L, purified water 1000mL.

[0030] Escherichia coli chromogenic medium was prepared in the same manner as in Example 1.

Embodiment 3

[0032] Escherichia coli chromogenic medium components include: agar 13g / L, peptone 8g / L, lactose 15g / L, sodium chloride 10g / L, bile salt 5g / L, 5-bromo-4-chloro-3- Indoxyl-N-acetyl-β-D-glucosamine 0.05g / L, purified water 1000mL.

[0033] Escherichia coli chromogenic medium was prepared in the same manner as in Example 1.

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Abstract

The invention relates to a chromogenic medium for identification of escherichia coli. The chromogenic medium contains one chromogenic substrate of 4-nitrophenyl-beta-D-glucopyranoside, o-nitrophenol beta-D glucuronide, 4-nitrophenyl-beta-D-glucopyranoside, 4-methylumbelliferyl-beta-D-glucuronide and 5-bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide, and the chromogenic substrate interacts with glucosaccharase of the escherichia coli to release chromogenic groups. With the adoption of the chromogenic medium for identification of the escherichia coli and the application of the chromogenicmedium, the components are simple, the operation is convenient, detection sensitivity and specificity are high, false positive and false negative are reduced, furthermore, the detection cycle is short, the chromogenic medium is applicable to treatment of large batches of samples, industrialized production is facilitated, and the escherichia coli in food can be detected comprehensively, symmetrically and accurately and identified primarily.

Description

technical field [0001] The invention relates to the technical field of detection and identification, in particular to Escherichia coli, in particular to a chromogenic medium for identification of Escherichia coli. Background technique [0002] Escherichia coli (Escherichia coli), also known as Escherichia coli, is a normal flora in the intestinal tract of humans and animals; but when the body's resistance decreases or it invades extraintestinal tissues or organs, it can cause extraintestinal infections; Escherichia coli Escherichia coli is an internationally recognized indicator bacteria for health monitoring, and the detection of Escherichia coli in food or water means direct or indirect recent fecal contamination. [0003] In standard inspection methods such as food and commodity inspection, eosin methylene blue agar (EMB), MacConkey agar, etc. are usually used to separate Escherichia coli, and combined with oxidase test, methyl red (MR) test, VP test, urea The identifica...

Claims

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Application Information

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IPC IPC(8): C12Q1/10
CPCC12Q1/10
Inventor 杨艳张明敏杨子江
Owner SHANGHAI JIKONG BIOTECH
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