38 results about "Glucosiduronates" patented technology

The invention discloses a pseudo-ginseng inducible promoter R1 and application thereof, the nucleotide sequence of R1 is shown as SEQ ID NO: 1, and molecular biology and genetic engineering related technical researches prove that the pseudo-ginseng promoter R1 responds to several plant hormones, biological stress and abiotic stress; a fusion expression cassette constructed by the panax notoginseng promoter R1 and the beta-glucuronidase gene is transferred into tobacco for expression, and the activity of the glucuronidase of the transgenic tobacco is quantitatively detected through a fluorescence method. The result shows that after the transgenic tobacco is treated by fusarium oxysporum, fusarium solani, alternaria alternata, NaCl, AlCl3, gibberellin, methyl jasmonate, salicylic acid, abscisic acid and ethephon, the activity of glucuronidase is obviously enhanced; the pseudo-ginseng promoter R1 is induced by several plant hormones and biological and abiotic stress factors, and can be used for plant disease-resistant gene engineering.

The invention discloses an inducible promoter PCHI which is derived from lilium regale. The nucleotide sequence of the inducible promoter PCHI is shown as SEQ ID NO: 1. According to the invention, technical research related to molecular biology and genetic engineering proves that the lilium regale promoter PCHI responds to jasmonic acid methyl ester and biological stress. An expression cassette constructed by the lilium regale promoter PCHI and a beta-glucosidase gene is transferred into tobacco for expression, and the glucosidase activity of the obtained transgenic tobacco is quantitatively detected through a fluorescence method; and results show that after the transgenic tobacco is treated with jasmonic acid methyl ester, fusarium oxysporum, fusarium solani and sporotrichum oryzae treatment, the glucosidase activity of the transgenic tobacco is obviously enhanced. The lilium regale promoter PCHI is induced by jasmonic acid methyl ester and biological stress factors and can be used in disease-resistant gene engineering of plants.

The invention discloses a lilium regale inducible promoter PG1. A nucleotide sequence of the lilium regale inducible promoter PG1 is as shown in SEQ ID NO:1. According to the invention, molecular biology and genetic engineering related technical researches prove that the lilium regale inducible promoter PG1 responds to a few plant hormones, organisms and abiotic stresses; a fusion expression cassette constructed by the lilium regale promoter PG1 and a beta-glucosidase gene is transferred into tobacco to be expressed; the glucosidase activity of transgenic tobacco is quantitatively detected through a fluorescence method; a result shows that after abscisic acid, salicylic acid, fusarium oxysporum, nigrospora oryzae, alternaria compact and damage stress treatment, the glucosidase activity of the transgenic tobacco is obviously enhanced; and the lilium regale promoter PG1 is induced by a few plant hormones, organisms and abiotic stress factors, and has a wide application prospect in biological or abiotic stress resistant genetic engineering.

The invention relates to application that a high-temperature resistant beta-glucuronide gene is obtained through combining the evolvement of oriented molecules of a gene in vitro and the mutation of a fixed point and is used as a report gene in plant gene transformation, and belongs to the field of plant gene engineering. The invention discloses a method for using the high-temperature resistant beta-glucuronide gene in the plant gene transformation and effect thereof. The high-temperature resistant beta-glucuronide gene can prevent interference of entogenous background of a transgenic organism and increase the reliability of transgenic detection; the high-temperature resistant beta-glucuronide gene is also used to obviously improve the sensitivity of transgenic detection so that the research result of expression and regulation induced by spatial specificity, biotic environment or abiotic environment has larger difference and more accurate data, thereby greatly reducing the use amount of a primer and reducing detection cost.

This invention provides a method for induced expression of beta-glucosidase in Lactobacillus casei 393. The method comprises: expressing beta-glucosidase in Lactobacillus casei 393 with surface-expression vector pPG611.1 and secretive expression vector pPG612.1. The target protein is generated in host cells. The expressed proteins are combined with cell walls, and do not exist in the supernatant. This invention also optimizes the induction conditions. The expression system can be used to study antigen presentation.

The invention discloses a method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef. The method includes hydrolyzing beef samples by the aid of beta-glucuronidase/aryl sulfatase; centrifuging the beef samples under the condition of pH (potential of hydrogen) of 1.0+/-0.2; regulating pH of supernatant until the pH reaches 9.5+/-0.2; sequentially extracting the supernatant by the aid of ethyl acetate and tert-butyl methyl ether; combining organic phases with one another; drying the organic phases by means of blowing; dissolving the organic phases by the aid of formic acid; enabling the organic phases to pass cation exchange solid-phase extraction columns; sequentially leaching the organic phases by the aid of 2% formic acid, water and methanol; eluting the organic phases by the aid of ammonium methanol; drying eluent by means of blowing; dissolving the organic phases by the aid of formic acid and methanol aqueous solution; filtering the organic phases by the aid of films; feeding filter liquid in liquid chromatograph/mass spectrometers to obtain the residual quantities of the growth promoting agents in the beef according to standard curves. The method has the advantages that the four growth promoting agents including the zilpaterol, the cimbuterol, the clenproperol and the bambuterol can be quickly detected by the aid of the method, and residues of the four growth promoting agents which are four types of medicine in the beef can be monitored.

The invention discloses an electronic ovulation predictor. The electronic ovulation predictor comprises an information processing unit for treating various data collected by the predictor, a sensing unit for detecting information and sending the information to a device processing the information, and an indication unit for indicating the existence of an ovum. The processed information comprises a basal body temperature, and the concentrations of E3G (Estrone-3-Glycuronide) and LH (Luteinizing Hormone) in first morning urine. After the information is processed, the results are compared with the associated data of the existence of the ovum, and if the ovum exists, an optical display sends out a signal to indicate the existence of the ovum. The electronic ovulation predictor is capable of quickly and effectively measuring the existence of the ovum.

The invention belongs to the technical field of plant gene engineering and particularly relates to a vector for verifying an enhancer and the application of the vector. The vector p3301-mini35S-GUS for verifying the enhancer comprises a GUS (beta-glucuronidase gene) report gene and a CaMV mini35S promoter sequence, and the vector has a nucleotide sequence of SEQ ID NO:11 as shown in the specification. The vector provided by the invention can be adopted to verify potential or candidate enhancer fragments in a gene promoter, study the action of a target fragment in enhancing gene expression, andfurthermore analyze function differences of promoters, can be further applied to analysis on gene functions, and particularly can be applied to study and development on equipotential gene function differences of plants.

The invention discloses a chromogenic medium used for detecting escherichia coli O157:H7 and other intestinal floras. The medium contains agar, peptone, a yeast extract, sodium chloride, sorbitol, inositol, neutral red, a beta-galactosidase chromogenic substrate, 4-methyl sector ketone-beta-D-glucuronide, isopropyl-beta-D-thiopyran galactoside, bile salt, potassium tellurite and cefixime. The chromogenic medium disclosed by the invention can be used for identifying escherichia coli O157:H7 in coliforms rapidly, simply and conveniently.

A genetic promoter with specific expression to vegetative trichome is disclosed. The sequence of the trichome-specific hydratropic proteinase inhibitor (SaPIN2b) gene promoter is cloned. The start site of SaPIN2b transcription is determined. Its plant expression carrier is configured. The test to its transgenic plant shows its specific expression in trichome.

The invention discloses a method for extracting and detecting prednisolone, aldosterone, testosterone and estradiol in Euphausia superb. The method comprises the steps of lyophilizing fresh Euphausiasuperb at 10-50 DEG C and then crushing the Euphausia superb; adding an acetic acid-sodium acetate buffer solution and a beta-glucuronidase to the crushed Euphausia superb to enzymatically hydrolyze;adding an acetonitrile or an ethyl acetate to an enzymatically hydrolyzed sample to extract in a vortex manner, extracting repeatedly for 3-4 times and merging supernates; performing extraction treatment by using a QuEChERS solid phase extraction column, purifying in a vortex manner, rotationally evaporating to be dry, redissolving by using the acetonitrile or the ethyl acetate, filtering by a 0.2[u]m filter membrane to obtain an extract of the prednisolone, the aldosterone, the testosterone and the estradiol in the Euphausia superb; and detecting with combined use of an ultra-high performance liquid chromatography-mass spectrography. According to the method provided by the invention, the operation in an extraction process is simple, and the consuming time is short; moreover, impurities affecting the detection can be fully removed; and therefore, the method is a method which is simple, feasible, short in the consumed time and high in an extraction rate.

The invention discloses a compound as well as a preparation method and application thereof, and belongs to the field of medicine. A name of the compound is (7'R,8S,8'R)-4'-hydroxy-3,3'-dimethoxy-9'-oxo-8-8',9'-O-9-lignans, wherein R is an R configuration and S is an S configuration. The preparation method comprises the following steps: wetting Illicium majus branches and leaves by using ethanol, and performing extraction to obtain an extraction solution; performing concentration on the extraction solution to obtain extract; dispersing the extract by distilled water, performing extraction by using equal-volume ethyl acetate, respectively, recovering the ethyl acetate and obtaining a product of crude extraction; and performing refining on the product of the crude extraction to obtain the compound. The compound disclosed by the invention can better inhibit PAF-stimulated polymorphonuclear leukocyte glucuronidase release and has obvious inhibition activity on Fe<2+> cysteine-induced livermicrosomal lipid peroxidation, and therefore the compound has better anti-inflammatory activity and anti-oxidation effects, and can be used as anti-inflammatory and anti-oxidation drugs.

The invention provides a transgenic soybean indicating auxin concentrations. The soybean repeats construction of DR5V2 promoters many times by using short promoter regions of auxin responding key genes, and beta-glucosidase (GUS) reporter genes are connected. The transgenic soybean can respond to the change of the auxin concentrations. After a GUS substrate, namely, 5-bromine-4-chlorine-3-indole-beta-gluconate(X-gluc), is stained, the distribution areas and concentration change of the auxin can be observed by a microscopy. Compared with a previously published soybean-auxin indicator plant pGH3-GUS, the provided DR5-V2-GUS transgenic soybean is more accurate in auxin indicating.

The invention provides a method for purifying flavone in purple perilla by utilizing a low-pressure liquid phase. The purple perilla flavone is obtained by preparing purple perilla flavone crude extracting liquid and performing D152 macroporous resin separation and purification. By adopting the method for purifying the flavone in purple perilla by utilizing the low-pressure liquid phase, chemicalcomponents possibly contained by a sample obtained after purification are identified by adopting high-resolution ion mobility liquid chromatograph/mass spectrometer as follows: apigenin 6,8-diglucoside, kaempferol 3-O-beta-glucoside-7-O-beta-glucuronic acid, luteolin-7-oxy-diglucuronide, apigenin-7-oxy-diglucuronic acid, kaempferol-3-O-glucosidic acid, caffeic acid tetramer, apigenin-5-oxy-glucuronic acid, rosemary acid, maleic acid, caffeic acid and the like. Only the elution speed exists, no adsorption speed exists, and the operation is relatively simple. The volume fraction of ethanol is low, and the cost can be saved.

The invention discloses a synthesizing method of deuterium-labeled glucuronide fenretinide. The synthesizing method comprises the following steps of 4-nitrophenol-D4 is used as a deuterium-labeled initiating matter, and six-step reaction is performed, so as to obtain the deuterium-labeled glucuronide fenretinide-D4. The synthesizing method has the advantages that the optimum preparation steps and reaction conditions are screened by a large amount of experiments, the whole technological design is reasonable, and the operability is strong; for the prepared deuterium-labeled glucuronide fenretinide, the purity can reach 98% or above, the yield rate can reach 70% or above, and the isotope abundance is greater than 99%. The prepared deuterium-labeled glucuronide fenretinide can be used as a deuterium-labeled metabolite of fenretinide, a test and contrast sample is provided for the study of metabolism mechanism of fenretinide and the anti-tumor mechanism, and the important application value is realized.

The invention discloses anti-alcoholism beverage containing kiwi fruit and tea fungus, and a preparation method of the anti-alcoholism beverage, and relates to the technical field of health drink. The anti-alcoholism beverage containing the kiwi fruit and the tea fungus is prepared from the following raw materials in parts by weight: 10-30 parts of kiwi fruit, 1-10 parts of honey, 1-10 parts of sugar, 1-5 parts of tea fungus, 0.1-1 part of tea leaf and 50-100 parts of water. The anti-alcoholism beverage containing the kiwi fruit and the tea fungus, and the preparation method of the anti-alcoholism beverage mainly utilize the specific detoxification functional factor: glucuronic acid of the tea fungus; the glucuronic acid is combined with a toxic substance 'acetaldehyde' generated after alcohol (ethanol) enters a human body so as to become water-soluble glucuronide and is discharged outside the human body together with the acetaldehyde, so that a better effect of dispelling the effects of alcohol is achieved.