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38 results about "Glucosiduronates" patented technology

Ethylene responsive factor gene OsERF3 and application of promoter thereof in regulating and controlling development of rice roots

The invention discloses application of an ethylene responsive factor gene OsERF3 in regulating and controlling the development of rice roots. By using a yeast two-hybrid method, an ethylene responsive factor OsERF3 interacted with a published OsWOX11 protein is obtained by screening. By using an overexpression and small artificial interference RNA method, transformed rice corresponding to a gene is obtained, and a situation that the length of a main root and the number of crown roots of a plant subjected to gene overexpression are obviously increased in comparison with those of wild compared plants, and the length increasing of the main root is caused by the length increasing of cells in a maturation zone of a root tip, the length of the main root and the number of crown roots of a plant subjected to small artificial interference RNA transgenosis are obviously reduced in comparison with those of wild compared plants, and the length reducing of the main root is caused by the length reducing of cells in a meristematic zone and a maturation zone. Results show that the gene has a function of promoting the development of rice roots. A result of fusing the promoter of the gene with a report gene beta-glucuronidase shows that the gene is expressed in a meristematic zone of a root tip.
Owner:HUAZHONG AGRI UNIV

Pretreatment method for analyzing tetrabromobisphenol A in biologic urine

The invention discloses a pretreatment method for analyzing tetrabromobisphenol A in biologic urine, relates to a detection and analysis technique of organic pollutant in a biologic sample, and provides a pretreatment technique for testing the content of tetrabromobisphenol A in urine. The pretreatment method comprises the following steps: regulating the pH value in a urine sample, performing enzymolysis on the urine in a mode of adding beta-glucuronide / aryl sulfuric acid lipase, enriching enzymatic hydrolysate through an ENVI-18 column, washing the enriched eluant by using a 1% hydrochloric acid solution and dichloromethane in a vortex and shaking manner, and subsequently washing a recycled organic layer by using distilled water for another time. The detection on the sample by using an ultra-high performance liquid chromatography-tandem mass spectrometer shows that the method is simple to operate, high in recycling rate and good in impurity moving effect, is applicable to pretreatment on the biologic urine sample, and is particularly applicable to extraction and purification operation on a large-scale biologic urine sample. By adopting the method, conjugate of the tetrabromobisphenol A in the sample can be effectively decomposed, the urine sample can be effectively purified, and support is provided for detection on the tetrabromobisphenol A in the urine.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI

Genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of biochemical site marker

ActiveCN107870190AEasy to operateFast intuitive homozygosityMaterial analysis by electric/magnetic meansHemoglobin Beta ChainTranslocase
The invention relates to a genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of the biochemical site marker. The genetic biochemical site marker for the cerebral ischemia inbred line meriones is selected from at least one of glucose-6-phosphate dehydrogenase-1, esterase-3, carbonic anhydrase-2, alkaline phosphatase-1, isocitrate dehydrogenase-1, malic enzyme-1, glucose phosphate translocase-1, renal catalase-2, peptidase-3, glucose phosphate isomerase-1, hemoglobin-beta chain, transferring, esterase-1, esterase-10, glycerol phosphate dehydrogenase-1, beta-glucuronidase-1, esterase-2, lactic dehydrogenase regulator-1, serum protein-1, amylase-1, esterase-6, esterase-8, esterase-9, esterase-4, catalase-1 and esterase-12.
Owner:ACADEMY OF MILITARY MEDICAL SCI

Double-antibody sandwich method for detecting beta-glucuronidase in escherichia coli cells of food

The invention discloses a double-antibody sandwich method for detecting beta-glucuronidase in escherichia coli cells of food, belonging to the technical field of immunoassay. The double-antibody sandwich method provided by the invention comprises the following steps of carrying out immune on seven-week BALB / c mice by using escherichia coli beta-glucuronidase (EC3.2.1.31), and fusing and screening to obtain ten monoclonal antibodies, respectively marking HRP and carrying out pairing; and establishing an sandwich ELISA analytical method by utilizing CGMCC No.7209 as a coating antibody, utilizing a CGMCC No.7211 as an enzyme labelled antibody, and utilizing the recombination expressive escherichia coli beta-glucuronidase as a standard substance. According to the double-antibody sandwich method provided by the invention, the beta-glucuronidase in escherichia coli ATCC 25922 detection cells is cracked, the escherichia coli is detected, and the LOD is 3.27*10^4cfu / mL; according to the method, the monoclonal antibody of a landmark of the escherichia coli, namely beta-glucuronidase, is prepared, the double-antibody sandwich method for detecting the escherichia coli is established, the method and salmonella, E.coli, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes do not have cross reaction, and the new detection method is provided for detecting the escherichia coli in food.
Owner:JIANGNAN UNIV

Method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef

The invention discloses a method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef. The method includes hydrolyzing beef samples by the aid of beta-glucuronidase/aryl sulfatase; centrifuging the beef samples under the condition of pH (potential of hydrogen) of 1.0+/-0.2; regulating pH of supernatant until the pH reaches 9.5+/-0.2; sequentially extracting the supernatant by the aid of ethyl acetate and tert-butyl methyl ether; combining organic phases with one another; drying the organic phases by means of blowing; dissolving the organic phases by the aid of formic acid; enabling the organic phases to pass cation exchange solid-phase extraction columns; sequentially leaching the organic phases by the aid of 2% formic acid, water and methanol; eluting the organic phases by the aid of ammonium methanol; drying eluent by means of blowing; dissolving the organic phases by the aid of formic acid and methanol aqueous solution; filtering the organic phases by the aid of films; feeding filter liquid in liquid chromatograph/mass spectrometers to obtain the residual quantities of the growth promoting agents in the beef according to standard curves. The method has the advantages that the four growth promoting agents including the zilpaterol, the cimbuterol, the clenproperol and the bambuterol can be quickly detected by the aid of the method, and residues of the four growth promoting agents which are four types of medicine in the beef can be monitored.
Owner:LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS

Method for extracting and detecting prednisolone, aldosterone, testosterone and estradiol in Euphausia superba

The invention discloses a method for extracting and detecting prednisolone, aldosterone, testosterone and estradiol in Euphausia superb. The method comprises the steps of lyophilizing fresh Euphausiasuperb at 10-50 DEG C and then crushing the Euphausia superb; adding an acetic acid-sodium acetate buffer solution and a beta-glucuronidase to the crushed Euphausia superb to enzymatically hydrolyze;adding an acetonitrile or an ethyl acetate to an enzymatically hydrolyzed sample to extract in a vortex manner, extracting repeatedly for 3-4 times and merging supernates; performing extraction treatment by using a QuEChERS solid phase extraction column, purifying in a vortex manner, rotationally evaporating to be dry, redissolving by using the acetonitrile or the ethyl acetate, filtering by a 0.2[u]m filter membrane to obtain an extract of the prednisolone, the aldosterone, the testosterone and the estradiol in the Euphausia superb; and detecting with combined use of an ultra-high performance liquid chromatography-mass spectrography. According to the method provided by the invention, the operation in an extraction process is simple, and the consuming time is short; moreover, impurities affecting the detection can be fully removed; and therefore, the method is a method which is simple, feasible, short in the consumed time and high in an extraction rate.
Owner:SHANDONG NORMAL UNIV

A double-antibody sandwich method for the detection of Escherichia coli intracellular β-glucuronidase in food

The invention discloses a double-antibody sandwich method for detecting beta-glucuronidase in escherichia coli cells of food, belonging to the technical field of immunoassay. The double-antibody sandwich method provided by the invention comprises the following steps of carrying out immune on seven-week BALB / c mice by using escherichia coli beta-glucuronidase (EC3.2.1.31), and fusing and screening to obtain ten monoclonal antibodies, respectively marking HRP and carrying out pairing; and establishing an sandwich ELISA analytical method by utilizing CGMCC No.7209 as a coating antibody, utilizing a CGMCC No.7211 as an enzyme labelled antibody, and utilizing the recombination expressive escherichia coli beta-glucuronidase as a standard substance. According to the double-antibody sandwich method provided by the invention, the beta-glucuronidase in escherichia coli ATCC 25922 detection cells is cracked, the escherichia coli is detected, and the LOD is 3.27*10^4cfu / mL; according to the method, the monoclonal antibody of a landmark of the escherichia coli, namely beta-glucuronidase, is prepared, the double-antibody sandwich method for detecting the escherichia coli is established, the method and salmonella, E.coli, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes do not have cross reaction, and the new detection method is provided for detecting the escherichia coli in food.
Owner:JIANGNAN UNIV
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