39 results about "Glucosiduronates" patented technology

The invention discloses a double-antibody sandwich method for detecting beta-glucuronidase in escherichia coli cells of food, belonging to the technical field of immunoassay. The double-antibody sandwich method provided by the invention comprises the following steps of carrying out immune on seven-week BALB / c mice by using escherichia coli beta-glucuronidase (EC3.2.1.31), and fusing and screening to obtain ten monoclonal antibodies, respectively marking HRP and carrying out pairing; and establishing an sandwich ELISA analytical method by utilizing CGMCC No.7209 as a coating antibody, utilizing a CGMCC No.7211 as an enzyme labelled antibody, and utilizing the recombination expressive escherichia coli beta-glucuronidase as a standard substance. According to the double-antibody sandwich method provided by the invention, the beta-glucuronidase in escherichia coli ATCC 25922 detection cells is cracked, the escherichia coli is detected, and the LOD is 3.27*10^4cfu / mL; according to the method, the monoclonal antibody of a landmark of the escherichia coli, namely beta-glucuronidase, is prepared, the double-antibody sandwich method for detecting the escherichia coli is established, the method and salmonella, E.coli, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes do not have cross reaction, and the new detection method is provided for detecting the escherichia coli in food.

The invention discloses the application of an ethylene response factor gene OsERF3 in regulating rice root development. An ethylene response factor OsERF3 that interacts with a published OsWOX11 protein is screened through a yeast two-hybrid method. Through the method of overexpression and artificial small interfering RNA, the corresponding transformed rice of the gene was obtained, and it was found that the length of the main root and the number of crown roots of the overexpressed gene were significantly increased and increased compared with the wild-type control plant, and the growth of the main root was due to the root tip The length of the main root and the number of crown roots of the artificial small interfering RNA transgenic plants were significantly shorter and less than those of the wild-type control plants, and the shortening of the main root was due to the length of the cells in the meristematic and mature zones. small cause. It shows that the gene has the function of promoting rice root development. Fusion of the promoter of this gene to the reporter gene β‑glucuronidase indicates that the gene is expressed in the root apical meristem.

The invention discloses a lilium regale inducible promoter LrP1 and application thereof. A nucleotide sequence of the LrP1 is as shown in SEQ ID NO: 1. According to the lilium regale inducible promoter LrP1 disclosed by the invention, the technical research related to molecular biology and gene engineering verifies that the lilium regale inducible promoter LrP1 is in response to several plant hormones and biotic and abiotic stress; expression cassettes constructed by connecting the lilium regale inducible promoter LrP1 disclosed by the invention and beta-glucuronidase genes are shifted into tobacco for expression, the activity of glucuronidase of transgene tobacco can be quantitatively detected through a fluorescence method, and a result shows that the activity of the glucuronidase is remarkably enhanced after the transgene tobacco is treated by gibberellin, ethylene, abscisic acid, NaCl, damage factors, fusarium oxysporum, sclerotinia sclerotiorum and botrytis cinerea; therefore, the lilium regale inducible promoter LrP1 is induced by several hormones and biotic and abiotic stress factors, so that the lilium regale inducible promoter LrP1 can be used for plant stress-resistant gene engineering.