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Method for purifying flavone in purple perilla by utilizing low-pressure liquid phase

A technology of perilla flavonoids and liquid phase, applied in chemical instruments and methods, separation methods, solid adsorbent liquid separation, etc., can solve the problems of high cost, complicated operation, low purity, etc., and achieve cost saving, low volume fraction, Simple operation effect

Pending Publication Date: 2018-12-28
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preparative chromatography only describes the rough division into two components, without specifying which components are divided into; both adsorption flow rate and elution flow rate, the operation is more complicated; the purity is not high; the volume fraction of ethanol is relatively high, and the cost is high

Method used

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Examples

Experimental program
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Embodiment 1

[0017] (1) Soak D152 macroporous resin in 95% ethyl alcohol for 24 hours to fully swell, then soak and rinse with purified water, 2% (w / v) NaOH solution, purified water, 2% (v / w) HCl solution in sequence, and rinse with Wash with purified water to pH 7 and set aside.

[0018] (2) Before loading the resin, a certain amount of ultrapure water must be filled in the glass chromatography column, mainly to prevent gas from entering the resin and affecting the column efficiency. Measure about 80mL of pretreated wet D152 macroporous resin, put the wet D152 macroporous resin into a ø5cm×24cm glass chromatography column along the glass rod, and the resin will finally settle to 2 / 3 of the column volume, about 16 cm (note that the liquid on the resin surface should not drain dry during the process of packing the column and using the column in the future, so as to prevent air bubbles from entering the inside of the resin and affecting the separation effect). The eluent (61% (v / v) ethanol)...

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PUM

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Abstract

The invention provides a method for purifying flavone in purple perilla by utilizing a low-pressure liquid phase. The purple perilla flavone is obtained by preparing purple perilla flavone crude extracting liquid and performing D152 macroporous resin separation and purification. By adopting the method for purifying the flavone in purple perilla by utilizing the low-pressure liquid phase, chemicalcomponents possibly contained by a sample obtained after purification are identified by adopting high-resolution ion mobility liquid chromatograph / mass spectrometer as follows: apigenin 6,8-diglucoside, kaempferol 3-O-beta-glucoside-7-O-beta-glucuronic acid, luteolin-7-oxy-diglucuronide, apigenin-7-oxy-diglucuronic acid, kaempferol-3-O-glucosidic acid, caffeic acid tetramer, apigenin-5-oxy-glucuronic acid, rosemary acid, maleic acid, caffeic acid and the like. Only the elution speed exists, no adsorption speed exists, and the operation is relatively simple. The volume fraction of ethanol is low, and the cost can be saved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for purifying perilla flavonoids by using a low-pressure liquid phase. Background technique [0002] Perilla has been planted and used in China for nearly 2,000 years. It is mainly used for medicine, oil, spices, food, etc. Its leaves (perilla leaves), stems (perilla stems) and fruits (perilla seeds) can be used as medicines. , the young leaves can be eaten raw and made into soup, and the stems and leaves can be soaked. In modern times, perilla has become a multi-purpose plant that has attracted worldwide attention due to its unique active substances and nutritional components, with high economic value. In recent years, as a new resource plant, perilla has been paid more and more attention, and the research on its development and utilization is becoming a hot spot in the world. [0003] Perilla is rich in nutrients, and the leaves contain rich carotenoids; stem...

Claims

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Application Information

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IPC IPC(8): B01D15/12B01D15/20B01D15/42
CPCB01D15/12B01D15/203B01D15/426
Inventor 张桂清刘仁玉兰思仁林占熺
Owner FUJIAN AGRI & FORESTRY UNIV
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