13 results about "Para-nitrophenyl" patented technology

The invention relates to the technical field of coatings, in particular to a coating containing a novel ultraviolet light absorber and a preparation method of the coating. The coating is prepared from the following raw materials in parts by weight: 45 to 55 parts of aliphatic polyurethane diacrylate, 22 to 28 parts of isobornyl acrylate, 12 to 18 parts of 1, 6-hexanediol diacrylate, 2.5 to 3.5 parts of novel ultraviolet light absorber, 1.6 to 2.4 parts of photoinitiator 1173, 0.8 to 1.2 parts of photoinitiator TPO and 36 to 44 parts of propylene glycol methyl ether acetate. The absorbent is obtained by activating a triazine-phenol structure through p-nitrophenyl chloro carbonate, coupling the triazine-phenol structure with 6-amino-6-deoxy-beta-cyclodextrin and then performing epoxidation, amphoteric chain segment grafting and methacryloylation, and site immobilization, amphoteric regulation and polymerizable anchoring of an ultraviolet absorption unit are achieved. The obtained coating has excellent adhesive force, hardness and bending resistance on a low-surface-energy membrane material, is high in ultraviolet shielding rate and stable in long-term weather resistance, and is suitable for the field of outdoor protective coatings.

The invention provides a biosensor, a nucleic acid detection and typing system based on a CRISPR system, a detection method and application, and belongs to the technical field of biological detection, alkaline phosphatase is adopted for catalyzing hydrolysis of p-aminophenyl phosphate to obtain p-aminophenol, the p-aminophenol is used for reducing silver ions, metal silver is deposited on the surfaces of gold nanoparticles, and the gold nanoparticles can be used for detecting the nucleic acid. Compared with a traditional Cas12a detection method combining ALP mediated p-nitrophenyl phosphate hydrolysis, the CRISPR / Cas12a biosensing strategy combining ALP has the advantages that the sensitivity is improved by 10,000 times, target DNA can be detected through naked eyes, the detection limit is as low as 300 aM, and the potential of no instrument application is shown.

The present invention relates to a galantamine analog having a cholinesterase inhibitory activity, and a preparation method therefor and the use thereof. The galantamine analog has a general structural formula as shown in formula (IA), (IB) or (IC). In formula (IA), R1 is selected from hydrogen, o-fluoro, m-fluoro, p-fluoro, 2,4-difluoro, m-trifluoromethyl, p-methyl, 3,5-dimethoxy, p-chloro, p-nitro, p-cyano or p-trifluoromethoxy. In formula (IB), R2 is selected from phenyl, o-fluorophenyl, m-fluorophenyl, p-fluorophenyl, 2,4-difluorophenyl, 3,5-difluorophenyl, o-methylphenyl, p-methylphenyl, p-methoxyphenyl, indole, p-cyanophenyl, p-trifluoromethylphenyl, p-trifluoromethoxyphenyl, p-nitrophenyl or naphthalene ring. In formula (IC), R3 is selected from hydrogen, o-fluoro, m-fluoro, p-fluoro, m-chloro, p-chloro, p-methyl, o-methoxy, m-methoxy or p-methoxy. The galantamine analog of the present invention has a cholinesterase inhibitory activity superior to that of galantamine, and is expected to be used for treating diseases associated with cholinergic dysfunction.

This invention relates to a galantamine analogue with cholinesterase-inhibiting activity, its preparation method, and its application. The general structural formula is shown in formula (IA), (IB), or (IC). In formula (IA), R1 is selected from hydrogen, o-fluorine, m-fluorine, p-fluorine, 2,4-difluoro, m-trifluoromethyl, p-methyl, 3,5-dimethoxy, p-chloro, p-nitro, p-cyano, or p-trifluoromethoxy. In formula (IB), R2 is selected from phenyl, o-fluorophenyl, m-fluorophenyl, p-fluorophenyl, 2,4-difluorophenyl, 3,5-difluorophenyl, o-methylphenyl, p-methylphenyl, p-methoxyphenyl, indole, p-cyanophenyl, p-trifluoromethylphenyl, p-trifluoromethoxyphenyl, p-nitrophenyl, or a naphthalene ring. In formula (IC), R3 is selected from hydrogen, o-fluorine, m-fluorine, p-fluorine, m-chloro, p-chloro, p-methyl, o-methoxy, m-methoxy, or p-methoxy. The galantamine analogue of this invention exhibits superior inhibitory activity against cholinesterase compared to galantamine, and holds promise for the treatment of cholinergic dysfunction-related diseases.

The invention discloses thermophilic beta-1, 4-glucosidase and an application of the thermophilic beta-1, 4-glucosidase. The base sequence of the thermophilic beta-1, 4-glucosidase is as shown in SEQ ID NO: 1. The thermophilic beta-glucosidase provided by the invention can be used for hydrolyzing p-nitrophenyl-beta-D glucopyranoside (pNPGlu), p-nitrophenyl-beta-D galactopyranoside (pNPGal), p-nitrophenyl-beta-D xylopyranoside (pNPXyl), p-nitrophenyl-alpha-D glucopyranoside (pNP alpha Glu), p-nitrophenyl-N-acetylglucosamine, geniposide, polydatin, glucosyltransferase, glucosyltransferase and the like. And salicin and arbutin. The optimum reaction temperature of the enzyme is 95 DEG C, and after the enzyme is treated at 70 DEG C for 24 hours, the residual enzyme activity is greater than 40%. In an n-hexyl alcohol-water phase (or n-caprylic alcohol-water phase), geniposide can be hydrolyzed into genipin, and industrial production of genipin is facilitated.

The amino acid sequence of the novel thermophilic alpha-L-rhamnosidase is shown as SEQ ID No.1, and the novel thermophilic alpha-L-rhamnosidase is derived from a metagenome of a hot spring in Yuanjiang County in Yunnan province. The thermophilic alpha-L-rhamnosidase YJ-Rha72 provided by the invention can hydrolyze flavonoid substances such as rutin, naringin, hesperidin, neohesperidin and the like and p-nitrophenyl-alpha-L-rhamnopyranoside (pNPR), the rutin hydrolysis activity is the highest, and the thermophilic alpha-L-rhamnosidase YJ-Rha72 has the hydrolytic activity of alpha-1, alpha-1, 2 and alpha-1, 6 glucosidic bonds. The optimum reaction temperature of the enzyme is 75 DEG C, and the activity of the enzyme at 50-85 DEG C exceeds 34.9%; the optimum reaction pH is 6.0, and the activity exceeds 74% in the pH range of 5-7.5. The enzyme can completely hydrolyze 1 mM of rutin within 20 minutes, and the conversion rate is 100%. The alpha-L-rhamnosidase YJ-Rha72 has important application potential and value, for example, the alpha-L-rhamnosidase YJ-Rha72 is used for preparing important bioactive substances such as isoquercitrin and Prunin through biotransformation of rhamnoside substances, debitterizing citrus juice, enhancing aroma of grape wine and the like.

This invention relates to a galantamine analogue with cholinesterase-inhibiting activity, its preparation method, and its application. The general structural formula is shown in formula (IA), (IB), or (IC). In formula (IA), R1 is selected from hydrogen, o-fluorine, m-fluorine, p-fluorine, 2,4-difluoro, m-trifluoromethyl, p-methyl, 3,5-dimethoxy, p-chloro, p-nitro, p-cyano, or p-trifluoromethoxy. In formula (IB), R2 is selected from phenyl, o-fluorine, m-fluorine, p-fluorine, 2,4-difluorophenyl, 3,5-difluorophenyl, o-methylphenyl, p-methylphenyl, p-methoxyphenyl, indole, p-cyanophenyl, p-trifluoromethylphenyl, p-trifluoromethoxyphenyl, p-nitrophenyl, or a naphthalene ring. In formula (IC), R3 is selected from hydrogen, o-fluorine, m-fluorine, p-fluorine, m-chloro, p-chloro, p-methyl, o-methoxy, m-methoxy, or p-methoxy. The galantamine analogue of this invention has superior inhibitory activity against cholinesterase compared to galantamine, and is expected to be used to treat cholinergic dysfunction-related diseases.

The invention discloses thermophilic beta-1, 4-glucosidase and an application of the thermophilic beta-1, 4-glucosidase. The amino acid sequence of the thermophilic beta-1, 4-glucosidase is as shown in SEQ ID NO: 1. The thermophilic beta-glucosidase provided by the invention can be used for hydrolyzing p-nitrophenyl-beta-D glucopyranoside (pNPGlu), p-nitrophenyl-beta-D galactopyranoside (pNPGal), p-nitrophenyl-beta-D xylopyranoside (pNPXyl), p-nitrophenyl-alpha-D glucopyranoside (pNP alpha Glu), p-nitrophenyl-N-acetylglucosamine, geniposide, polydatin, glucosyltransferase, glucosyltransferase and the like. And salicin and arbutin. The optimum reaction temperature of the enzyme is 90 DEG C, and after the enzyme is treated at 70 DEG C for 48 hours, the residual enzyme activity is greater than 95%, so that the industrial application of preparing the blue pigment by adopting a one-pot method under a high-temperature condition is facilitated.

The invention relates to oligomeric urea-amine molecules, preparation and application in carbon dioxide adsorption, and belongs to the technical field of high polymer materials. The preparation method comprises the following steps: firstly, reacting an amino-containing compound with p-nitrophenyl chloroformate to generate carbamate with a specific structure, and then reacting carbamate with hydrazine hydrate to combine amine groups in carbamate with hydrazine molecules in hydrazine hydrate to obtain oligomeric urea-amine molecules. The molecule has the characteristics of simple structure, excellent water solubility and high melting point, and can efficiently adsorb carbon dioxide; and after heating treatment at a relatively low temperature, desorption release of carbon dioxide can be realized, and regeneration of molecules can also be achieved.

The invention relates to a self-assembly oligopeptide mimic enzyme, a polypeptide-metal ion composite mimic enzyme and application thereof.The self-assembly oligopeptide mimic enzyme prepared through the method takes self-assembly polypeptide as a raw material, a nanobelt microstructure with the regular width is formed through self-assembly, and compared with the common protein disordered aggregation and amyloid protein fiber morphology in natural enzymes, the nanoribbon microstructure has the advantages that the structure is simple, and the application range is wide. The catalyst has a larger active site exposure space, so that the catalyst has high catalytic activity, and shows more excellent catalytic activity on decomposition of 4-nitrophenyl acetate and L-type dopa after being coordinated with different metal ions. Stability tests show that the self-assembled oligopeptide mimic enzyme prepared by the invention has high stability of dilution, freezing, acid-base and the like, and compared with natural enzymes, the self-assembled oligopeptide mimic enzyme is more suitable for catalytic reaction in more complex and harsh reaction environments.

The application relates to a nitro catalytic hydrogenation sulfur-resistant catalyst and a preparation method and application thereof, and belongs to the technical field of nickel-based catalyst preparation. The catalyst is prepared by adopting a deposition precipitation method and takes nickel as an active component, adds a certain amount of molybdenum additives and takes diatomite as a carrier; the catalyst is suitable for a catalytic hydrogenation production process of a p-nitrophenyl-beta-hydroxyethyl sulfone or m-nitrophenyl-beta-hydroxyethyl sulfone. The catalyst can be recycled and applied for multiple times while maintaining high activity and high selectivity, the efficiency of the catalyst is improved with the increase of the molybdenum content, and the catalyst can be recycled and applied for multiple times, which shows that the addition of molybdenum is beneficial to the sulfur resistance of the catalyst. The catalyst has the characteristics of high catalytic activity, good stability and easy separation; compared with a traditional Raney nickel catalyst, the catalyst can still maintain high activity after being used for multiple times, and has important significance for realizing the nitro catalytic hydrogenation sulfur resistance.

This invention relates to the field of coating technology, specifically to a coating containing a novel ultraviolet absorber and its preparation method. The raw materials of this coating, by weight, include 45–55 parts of aliphatic polyurethane diacrylate, 22–28 parts of isobornyl acrylate, 12–18 parts of 1,6-hexanediol diacrylate, 2.5–3.5 parts of the novel ultraviolet absorber, 1.6–2.4 parts of photoinitiator 1173, 0.8–1.2 parts of photoinitiator TPO, and 36–44 parts of propylene glycol methyl ether acetate. The absorber is obtained by activating the triazine-phenol structure with p-nitrophenyl chlorocarbonate and coupling it with 6-amino-6-deoxy-β-cyclodextrin, followed by epoxidation, amphoteric segment grafting, and methacrylamide, thereby achieving site immobilization, amphoteric regulation, and polymerizable anchoring of the ultraviolet absorption unit. The resulting coating exhibits excellent adhesion, hardness, and bending resistance on low surface energy films, high ultraviolet shielding efficiency, and long-term weather stability, making it suitable for outdoor protective coating applications.

The invention discloses a salt activated beta-galactosidase mutant E154V and application thereof. The amino acid sequence of the mutant is shown as SEQ ID NO. 1. In a system without adding salt, the hydrolytic activity of E154V to a substrate p-nitrophenyl-beta-D-galactopyranoside (pNPG) is lower, the activity of E154V in a system with salt is remarkably improved, and in a 500 mM potassium acetate solution, the hydrolytic activity of the mutant to the pNPG is improved by 11.41 times and is improved by 124.26% compared with that of a recombinant wild enzyme without adding salt; the hydrolytic activity to galactooligosaccharide is improved by 22.13 times, and is improved by 58.72% compared with that of a recombinant wild enzyme without salt. The mutant is suitable for a technical application scene in which the enzymatic reaction rate needs to be adjusted, the reaction efficiency and controllability can be effectively improved, and a better solution is provided for enzyme catalysis application in the fields of agriculture, food processing, biotechnology and the like.