11 results about "Mitochondria targeted" patented technology

The present application relates to a kind of electron donor (alpha-trithiophene) modified rhodamine dye, its synthesis method and application.The dye has strong absorption of 500-600 nm, can emit efficiently in 550-700 nm band, suitable for deep tissue imaging.The structure endows mitochondria targeting ability, can be used as biomarker probe to realize subcellular organelle accurate positioning;Meanwhile, under light, it can efficiently produce reactive oxygen species through type I mechanism, suitable for the photodynamic regulation and biosensing for non-disease diagnosis and treatment purposes.In addition, the dye can also be used as organic photocatalyst for environmental or energy-related photocatalytic reaction.The present application provides a new choice for multi-scene optoelectronic functional materials.

The present application relates to the technical field of targeted nanoparticles, and particularly relates to a mitochondria-targeting autophagy-inhibiting polymer and a preparation method thereof, a nano delivery particle and a preparation method and application thereof. A high molecule TPP-PEG-SH (TPS) containing a triphenylphosphonium group (triphenylphosphonium, TPP) is coupled with a mitochondria autophagy inhibitor Mdivi-1 through a disulfide bond to construct a polymer TPSM with mitochondria targeting ability and GSH responsiveness, and further co-assembled with a photosensitizer IT-4Cl to form a nano delivery system TPSM@IT-4Cl. The nano delivery system has mitochondria targeting ability, promotes continuous accumulation of oxidative stress at a tumor site, and can realize responsive drug release in a high-concentration GSH tumor microenvironment.

The invention discloses a trace element chelating agent based on marine biogen carboxyl and mitochondrial targeting modification as well as a preparation method and application of the trace element chelating agent, and relates to the technical field of agricultural biotechnology and novel fertilizers. Raw material pretreatment: taking marine algae or marine invertebrate leftovers rich in polysaccharide and protein as raw materials; cleaning, degreasing, drying, and crushing to 80-120 meshes, so as to obtain marine biogen coarse powder; and controllable enzymolysis: mixing the marine biogen coarse powder with a phosphate buffer solution according to a mass-volume ratio of 1: 10-1: 20, and after being absorbed by plants, the marine biogen coarse powder has a tendency to enrich mitochondria. The characteristic is expected to promote the trace elements to be more directly conveyed to organelles needing the trace elements to participate in physiological processes such as energy metabolism, so that the utilization efficiency of the trace elements can be possibly improved.

The invention relates to the technical field of biological medicine, in particular to a compound and a preparation method thereof, a photo-thermal agent and application of the photo-thermal agent in obesity treatment. The compound has a structure as shown in a formula I in the specification, wherein R is selected from at least one of anthracene-containing groups, dibenzothiophene-containing groups or carbazole-containing groups, and X is selected from at least one of halogen atoms, PF6 <-> and BF4 <->. The compound provided by the invention has mitochondrial targeting property and good photothermal conversion capability, and can realize obesity treatment by promoting the browning process of white adipose tissues.

The invention provides a preparation method and application of mitochondria-loaded 3D stem cell spheres, and relates to the technical field of biomedicine, and the technical key points are that the preparation method of the mitochondria-loaded 3D stem cell spheres comprises the following steps: obtaining mitochondria; preparing a stem cell suspension loaded with mitochondria; and preparing the mitochondria-loaded 3D stem cell spheres. The 3D stem cell sphere prepared in the invention can better simulate an in-vivo microenvironment, has anti-oxidation and anti-inflammatory effects and the like, and is beneficial to resisting inflammation microenvironment stimulation. And the mitochondria as a cell energy and signal center can provide great benefits for disease treatment, and the antioxidant effect of the mitochondria can neutralize excessive mtROS, relieve oxidative stress and protect the cell structure from being damaged. Finally, by inhibiting generation of mtROS, activation of key inflammatory pathways such as NF-kappa B and the like can be blocked, and a strong anti-inflammatory effect is exerted. Through the synergistic effect of the three, the targeted mitochondria becomes a prospective strategy for treating various diseases from energy, oxidation and inflammation root sources.

The invention discloses a preparation method of an ROS response microsphere encapsulated mitochondrial targeted nootkatone loaded Mxene multifunctional composite material, and relates to the technical field of biomedicine.The preparation method comprises the steps that carboxylated Mxene nanosheets are prepared and dissolved in dimethyl sulfoxide to obtain a product MXene-COOH-NK, then the product MXene-COOH-NK is dissolved in a 2-morpholine ethanesulfonic acid MES solution to obtain a product MXene-COOH-NK-Man, and the MXene-COOH-NK-Man is loaded on the Mxene multifunctional composite material to obtain the ROS response microsphere encapsulated mitochondrial targeted nootkatone loaded Mxene multifunctional composite material. On the basis, the ROS response microsphere encapsulated mitochondrial targeted Mxene loaded nootkatone is prepared. MXene has near-infrared photothermal conversion performance and supports light controlled drug release and local mild thermal therapy, MXene-COOH at NK can directly enrich chondrocyte mitochondria after being modified by mannosamine, expression of inflammatory factors is further inhibited, boric acid ester bonds are contained in the microspheres, and the MXene-COOH at NK can be used for preparing a drug for treating chondrocyte mitochondria, so that the MXene-COOH at NK can be used for preparing a drug for treating chondrocyte mitochondria. The nootkatone is broken under the high ROS environment condition of an OA focus, fixed-point and on-demand release of the medicine nootkatone is achieved, and whole body exposure is reduced.

The application discloses a preparation method of ROS response microsphere encapsulated mitochondria targeted nocardicin loaded Mxene multifunctional composite material, relates to the technical field of biological medicine, and prepares carboxylated Mxene nanosheet, dissolves in dimethyl sulfoxide to obtain product MXene-COOH@NK, is dissolved in 2-morpholinoethanesulfonic acid MES solution to obtain product MXene-COOH@NK-Man, and on this basis, ROS response microsphere encapsulated mitochondria targeted Mxene loaded nocardicin is prepared. The ROS response microsphere encapsulated mitochondria targeted Mxene loaded nocardicin prepared by the application has the near-infrared light heat conversion performance of MXene to support light-controlled drug release and local mild thermotherapy, and after the modification of MXene-COOH@NK by mannose, the MXene-COOH@NK can be directly enriched with chondrocyte mitochondria, further inhibit the expression of inflammatory factors, the microsphere contains borate ester bonds, the borate ester bonds are broken under the high ROS environment condition of OA lesion, the site release of the drug nocardicin is realized, and the systemic exposure is reduced.

Near-infrared (NIR) absorbing photosensitizers for imaging and treatment of cancer in a photodynamic therapy (PDT) are provided. Specifically, the NIR photosensitizers are mitochondria targeted and water-soluble, and used as a cytotoxic drug in a photodynamic therapy of the cancer. The NIR photosensitizers are activated with wavelengths of light, wherein the light penetrates through a body not just a skin. Hence the NIR photosensitizers aim to transform PDT from being a specialized treatment to a generally applicable one. PDT is almost completely non-invasive compared to current treatment methods.

The application discloses an OCTN1-mitochondria double-targeting cascade delivery liposome of ergothioneine, a preparation method and application thereof, and belongs to the technical field of functional cosmetics and targeted drug preparations. The application adopts an ethanol injection method combined with a pH gradient active drug loading technology to prepare a core drug loading liposome, and sequentially modifies ceramide 3, an OCTN1 targeting ligand and a mitochondria targeting ligand on the surface, so as to construct a three-step cascade delivery system of efficient transdermal active cell uptake and mitochondria precise enrichment. The prepared liposome has an encapsulation efficiency of no less than 88%, a drug loading amount of no less than 9.8%, a 24h in-vitro transdermal cumulative permeation amount of no less than 15 mu g / cm 2 , an intracellular mitochondria enrichment rate of no less than 65%, and a mitochondria ROS clearance efficiency of no less than 87%, and the antioxidant and anti-photoaging effects of ergothioneine are significantly improved, so that the application can be widely applied to the fields of high-end anti-aging cosmetics, medical repair products and functional skin care products.

The invention belongs to the technical field of biological medicines, and discloses a damaged mitochondria targeting pyridinium-based nano material as well as preparation and application thereof. The invention relates to a damaged mitochondria targeting pyridinium-based nano material, which comprises more than one of the following formulas I-II, p1 is poly (4-vinylpyridine) or polyethylene acrylate pyridine in which a pyridine group participates in forming a pyridinium group; p2 is NH2 or a secondary amine group which participates in forming a polyamide-amine dendrimer, a polypropyleneimine dendrimer, a polylysine dendrimer, linear polyethyleneimine, branched polyethyleneimine and linear polylysine which contain an N group in a Z structure. The invention also discloses a preparation method of the pyridinium-based nano material. The pyridinium-based nano material is stable in structure, has good damaged mitochondrial targeting ability, mitochondrial autophagy promoting ability and biocompatibility, and is used for preparing drugs for treating mitochondrial dysfunction related diseases, including drugs for treating atherosclerosis and non-alcoholic fatty liver diseases.

The invention discloses a mitochondrial targeting type viscosity fluorescent probe as well as a synthesis method and application thereof, and belongs to the technical field of biological materials. The chemical structure of the probe is shown as a formula I. The mitochondrial targeting viscosity fluorescent probe provided by the invention targets a mitochondrial inner membrane, structurally comprises a twisted intramolecular charge transfer (TICT) fluorescent rotor, and can emit a fluorescence signal with adjustable intensity along with the change of microenvironment viscosity. Compared with the traditional mitochondrial dye, the probe does not depend on mitochondrial membrane potential, and has excellent light stability and ultrahigh resolution imaging capability. Cell experiments show that the probe can be used for Alzheimer's disease (AD) imaging, and can indicate mitochondrial inner membrane viscosity change in real time and reveal a dynamic process of interaction between mitochondria and lysosome; pathological characteristics such as mitochondrial viscosity increase, ridge structure disorder, network fragmentation and lysosomal dyskinesia are observed in an AD model.