8 results about "Subcellular organelle" patented technology

The present application relates to a kind of electron donor (alpha-trithiophene) modified rhodamine dye, its synthesis method and application.The dye has strong absorption of 500-600 nm, can emit efficiently in 550-700 nm band, suitable for deep tissue imaging.The structure endows mitochondria targeting ability, can be used as biomarker probe to realize subcellular organelle accurate positioning;Meanwhile, under light, it can efficiently produce reactive oxygen species through type I mechanism, suitable for the photodynamic regulation and biosensing for non-disease diagnosis and treatment purposes.In addition, the dye can also be used as organic photocatalyst for environmental or energy-related photocatalytic reaction.The present application provides a new choice for multi-scene optoelectronic functional materials.

The present application relates to a multi-color fluorescence fluctuation imaging system and method based on a slowly varying transmittance dichroic mirror, which comprises: using multiple wavelength quantum dot fluorescence to mark different subcellular organelle structures, and using a single wavelength excitation light to obtain multi-color fluorescence signals; using a slowly varying transmittance dichroic mirror to split the multi-color fluorescence signals, and using a single camera to simultaneously collect multi-color fluorescence signals in the reflection channel and the transmission channel; using a method based on high-order correlation function calculation to separate different wavelength fluorescence signals; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using a multi-wavelength channel signal mutual iteration method to reduce cross-color between different channels; using

A method for detecting labile zinc (Zn2+) in a biological material is provided herein, the method including: (a) contacting the biological material with a composition including NapBu-BPEA; and (b) imaging the biological material via molecular fluorescence imaging to detect the labile zinc in the biological material. Also provided herein are methods for morphology-correlated detection of labile zinc localization in a subcellular organelle of a living cell and methods for tracking a change in labile zinc localization in a biological material.

The application belongs to the technical field of biological diagnosis, and relates to a subcellular organelle active targeting imaging probe and a preparation method thereof. The subcellular organelle active targeting imaging probe of the application is formed into nanoparticles (NPs) by coupling a fluorescent imaging probe with a special sequence of bombesin peptide and a derivative thereof; on one hand, the NPs can be surface-functionalized with lipophilic cations related to the NPs; on the other hand, the NPs can be passively targeted to tumor sites, and have high permeability and retention effect for solid tumors.

The invention relates to a method for detecting the state of a DNA nanostructure based on co-localization of a single dye label and a subcellular organelle, and the detection method comprises the following steps: incubating a DNA nanostructure connected with a dye and cells, and detecting the co-localization coefficient of the dye and the cellular organelle; when the co-localization coefficient of the dye and the lysosome is greater than 0.5, judging that the DNA nanostructure is complete; when the co-localization coefficient of the dye and the mitochondria is greater than 0.5, judging that the DNA nano structure is damaged; and judging that the DNA nanostructure is partially complete under other conditions. According to the method for judging the fate of the DNA nanostructure and detecting the integrity of the DNA nanostructure based on co-localization of the single dye and the subcellular organelle, not only can the intact or damaged states of the same DNA nanostructure under different conditions be distinguished by utilizing the position of the DNA nanostructure in the subcellular organelle in the cell, but also the intracellular states of different DNA nanostructures can be detected.

The invention relates to an electron donor (alpha-trithiophene) modified rhodamine dye as well as a synthesis method and application thereof. The dye has strong absorption of 500-600 nm, can efficiently emit in a wave band of 550-700 nm, and is suitable for deep tissue imaging. The structure endows mitochondria with targeting ability, and the probe can be used as a biomarker probe to realize precise positioning of subcellular organelles; meanwhile, active oxygen is efficiently generated through an I-type mechanism under illumination, and the compound is suitable for photodynamic regulation and biosensing for non-disease diagnosis and treatment purposes. In addition, the dye can also be used as an organic photocatalyst for environment or energy related photocatalytic reaction. The invention provides a new choice for a multi-scene photoelectric functional material.

This invention discloses a photosensitive nitric oxide donor that targets both lysosomes and mitochondria, its preparation method, and its applications, belonging to the fields of biomedical materials and medicinal chemistry. The nitric oxide donor has a structure as shown in formula DNOD560, which simultaneously includes a morpholino group for lysosome targeting and a rhodamine fluorophore for mitochondrial targeting, and uses an N-nitroso bond as the photosensitive nitric oxide release unit. The donor undergoes fluorescence quenching under physiological conditions and can undergo N-N bond cleavage under specific wavelength (e.g., 365 nm) illumination, quantitatively releasing nitric oxide and simultaneously generating the strongly fluorescent product DLMF560, enabling self-reporting and visualization of the release process. This donor has advantages such as high release efficiency (95% release efficiency within 70 minutes) and good photostability. Crucially, co-localization experiments have confirmed that it can simultaneously target lysosomes and mitochondria in cells, achieving in-situ, photosensitive release of nitric oxide at the subcellular organelle level, and has been successfully applied to live-cell (A549) imaging, providing a novel tool for studying the function of nitric oxide in subcellular organelles and developing precision treatment strategies.

The application belongs to the field of fluorescent probes, and discloses a kind of fluorescent probe for monitoring dynamic change of hydrogen peroxide in different subcellular organelles, preparation method and application thereof, the probe is with flavonol as parent nucleus, and hydrogen peroxide ratio type fluorescent probe PML is targeted to lysosome, and hydrogen peroxide ratio type fluorescent probe PZD is targeted to lipid droplet.The application also provides the preparation method and application of the probe.The probe provided by the application has novel structure, simple synthesis method, good recognition effect on hydrogen peroxide, especially hydrogen peroxide in subcellular organelles, fast response speed, high sensitivity, reliable inspection result, good biocompatibility, subcellular organelle positioning function, so that they can be used for monitoring hydrogen peroxide in intracellular microenvironment, and it has great significance to apply probe technology to practical related pathological model to develop the mechanism of hydrogen peroxide in its pathology, especially in the process of ferroptosis.