74 results about "Subcellular organelle" patented technology

The present invention provides methods and compositions related to the fields of chemoinformatics, chemogenomics, drug discovery and development, and drug targeting. In particular, the present invention provides subcellular localization signals (e.g., chemical address tags) that influence (e.g., direct) subcellular and organelle level localization of associated compounds (e.g., drugs and small molecule therapeutics, radioactive species, dyes and imagining agents, proapoptotic agents, antibiotics, etc) in target cells and tissues. The compositions of the present invention modulate the pharmacological profiles of associated compounds by influencing the compound's accumulation, or exclusion, from subcellular loci such as mitochondria, endoplasmic reticulum, cytoplasm, vesicles, granules, nuclei and nucleoli and other subcellular organelles and compartments. The present invention also provides methods for identifying chemical address tags, predicting their targeting characteristics, and for rational designing chemical libraries comprising chemical address tags.

Methods and compositions are disclosed to engineer plastids comprising heterologous genes encoding immuno-activating domains fused to an extracellular domain (ECD) of a receptor or surface glycoprotein, a growth factor or an enzyme and produced within a subcellular organelle, such as a chloroplast. The immuno-activating domains may include those regions of a protein capable of modulating the interaction between immune effector cells via proteins containing stereoselective binding domains and specific ligands, such as the Fc regions of antibodies. The present disclosure also demonstrates the utility of plants, including green algae, for the production of complex multi-domain fusion proteins as soluble bioactive therapeutic agents.

The invention relates to a ratiometric pH fluorescent probe as well as a preparation method and an application thereof. The chemical structural formula of the fluorescent probe is shown as formula I in the specification, wherein R is -OH or a subcellular organelle positioning receptor, and the subcellular organelle positioning receptor comprises either of two components shown in the specification. Compared with the prior art, the ratiometric pH fluorescent probe has good light stability, high selectivity, wide pH value detection range and high sensitivity, the preparation method is simple, and the ratiometric pH fluorescent probe can be used for detecting the pH value of a chemical system or the pH value in living cells of creatures.

Affinity separation compositions and methods are disclosed for separating targets from complex mixtures. Affinity reagents are bound to a solid support oriented in a manner to facilitate the activity of the affinity reagents which are capable of binding specific targets by affinity recognition. Affinity reagents include IgY antibodies, proteins, peptides, nucleotides and polymers. Targets include proteins, protein-protein complexes, protein-nucleotide complexes, nucleotides, cells and subcellular organelles.

The present invention relates to pH-tunable, highly activatable multicolored fluorescent nanoplatforms and methods of using the nanoplatforms in a variety of applications including, but not limited to, investigating fundamental cell physiological processes such as pH regulation in endocytic vesicles, endosome/lysosome maturation, and effect of pH on receptor cycling and trafficking of subcellular organelles.

Methods and compositions are disclosed to engineer chloroplast comprising heterologous genes encoding target binding domain fused to a eukaryotic toxin and produced within a subcellular organelle, such as a chloroplast. The present disclosure demonstrates that when chloroplasts are used, toxins normally refractive to production in eukaryotic cells may be used to produce recombinant fusion proteins with binding domains that are soluble, properly folded and post-translationally modified, where the multifunctional activity of the fusion protein is intact. The binding domains may include those from antibodies, receptors, hormones, cytokines, chemokines, and interferons. The present disclosure also demonstrates the utility of plants, including green algae, for the production of complex multi-domain proteins as soluble bioactive therapeutic agents.

The invention discloses a fluorescent probe for monitoring lipid peroxidation processes in different subcellular organelles, and belongs to the field of fine chemical engineering. The fluorescent probe is prepared through the steps of: enabling fluorine boron pyrrole derivatives to react with cinnamic aldehyde, wherein piperidine is used as a catalyst, and a 4 angstrom molecular sieve is used as a dehydrating agent; and performing quaternization on the obtained reactant so as to obtain molecules of the fluorescent probe. The probe contains active groups such as triphenylphosphine cations, morpholine and quaternary ammonium salt cations, the probe has a single-minded subcellular organelle targeting property because of the different active groups, and the probe can be optionally positioned on subcellular organelles such as mitochondria, lysosomes and cell membranes. The probe has zero toxin and zero side effects and is suitable for monitoring the lipid peroxidation processes in the subcellular organelles in different physiological processes through a fluorescent microscope or flow type cell analyzing technology. The probe is stable in solid condition nature and easy to store for a long time, an intuitional succinct detecting reagent with low cost is provided for inquiring processes relevant to the lipid peroxidation in cells, and microcosmic structural information is provided for detection by a subcellular organelle targeting property function.

The invention belongs to the field of pharmaceutic preparations in medical technology and discloses a difunctional tumor targeted liposome drug-delivery system and a preparation method and an application thereof. By directly coupling a cell-penetrating peptide rich in arginine residues and liposome surface long-chain organic acid and with the combination of a polyethylene glycol-hydrazone bond-long-chain organic acid conjugate, a tumor targeted liposome loading a fat soluble drug and a water-soluble anthraquinone slightly alkaline drug is prepared by a thin-film rehydration method, wherein the cell-penetrating peptide is polyarginine short peptide, preferably nonaarginine; molecular weight of polyethylene glycol is 500 Da-5000 Da, preferably 2000 Da; optimal cell-penetrating peptide density is 2-8% (preferably 4%) of density of phospholipid; and density of the polyethylene glycol-hydrazone bond-long-chain organic acid conjugate is 5-20% (preferably 8%) of density of phospholipid. The system provided by the invention solves the problem that existing liposome cellular internalization is difficult, and provides possibility for antitumor drug targeting subcellular organelle. The drug-delivery system basically is nontoxic, has a simple preparation technology and is easy for industrial application.

The present invention provides for proteome fractionation through the separation and accumulation of subcellar organelles from a biological sample such that the subcellular organelles are highly enriched, substantially pure, and whose structural integrity and functions are well-preserved. The methods of the invention provide a manner by which to reduce the complexity of the proteome and facilitate the detection and isolation of difficult-to-study proteins, such as low-abundance proteins. The methods of the present invention for pre-fractioning proteomes of biological samples by parallel separation and isolation of subcellular organelles from the biological samples using continuous-flow ultracentrifugation are also easily and effectively scalable through adjustment to ultracentrifugation parameters, such as, for example, rotor speed, rotor size, rotor geometry.

The invention discloses a hypoxia activation doxorubicin prodrug and a preparation method thereof. The doxorubicin prodrug is synthesized by modification of an amino group located at a 3' position ofdoxorubicin by an azo bond having a hypoxia activation effect and a response functional group having a self-occluded structure. The doxorubicin prodrug provided by the invention can effectively realize selective activation under a hypoxic environment, and can regulate and control growth activity of tumor cells by regulating subcellular organelle distribution of the doxorubicin in normoxia and hypoxic environments, thereby reducing toxic and side effects of a drug on normal cells, improving targeting efficiency of the drug on tumor treatment, and providing more novel ideas for development of antitumor drugs; and the synthetic method for the hypoxia activation doxorubicin prodrugs provided by the invention is simple, raw materials are cheap and easy to obtain, and the hypoxia activation doxorubicin prodrugs are convenient for industrialized production and clinical transformation.

The invention relates to a glutamine optical probe and a preparation method and application thereof. On one hand, the optical probe comprises glutamine sensitive polypeptide or a functional variant thereof and optically active polypeptide or a functional variant thereof, wherein the optically active polypeptide or the functional variant thereof is located in the sequence of the glutamine sensitivepolypeptide or functional variant thereof. The invention also relates to the preparation method of the probe and the application of the probe in the detection of amino acids. The glutamine optical probe has relatively smaller molecular weight and is easy to mature, has large fluorescence dynamic change and good specificity and can be expressed in different subcellular organelles of cells, and theamino acids can be quantitatively detected inside and outside the cells with high flux.

The invention discloses a database establishing method suitable for plant transposase accessible chromatin analysis. The database establishing method comprises following steps: firstly, plant tender tissue is subjected to pre-treatment, enzymatic hydrolysis is cell walls is carried out, filtering is carried out, DAPI dyeing is adopted to sort and collect nucleuses on a flow cytometer, the obtainednucleuses are filtered so as to reduce interference of cell walls and subcellular organelles on experiment data; Tn5 transposase digestion reaction and purification are carried out, qPCR is adopted to determine circulating number needed by database establishing; at last obtained single library is subjected to equimolar mixing. According to the database establishing method, the high quality on computer library is obtained via optimized two circles of magnetic cell sorting at a certain ratio, so that important experiment method reference is provided for study of scientific research personnel onplant tender tissue accessible transposase nucleus chromatin high flux sequencing.

Provided herein is a photonic bandgap (PBG) detector effective to detect inorganic molecules, organic biomolecules or biopolymers, cells, subcellular organelles, and particles. The PBG detector utilizes photonic crystals having a binding agent attached to channel surfaces comprising the crystals to selectively bind a molecule, cell or particle of interest so that an increase in light transmission is detectably induced within the photonic bandgap upon binding. Also provided are methods of optically detectiing an analyte and of identifying the presence of a cell or a particle in a biological sample.

The invention relates to an amino acid optical probe, and a preparation method and an application thereof. On one hand, the invention relates to an optical probe comprising an amino acid sensitive polypeptide or a functional variant thereof and an optical active polypeptide or a functional variant thereof, wherein the optical active polypeptide or the functional variant thereof is located in a sequence of the amino acid sensitive polypeptide or the functional variant thereof. The invention also relates to the preparation method of the probe and an application of the probe in detection of aminoacids. The amino acid optical probe provided by the invention has relatively small molecular weight, has the advantages of easy maturation, large fluorescence dynamic change and good specificity, canbe expressed in different subcellular organelles of cells, and can detect amino acids inside and outside cells in high flux and quantitatively.

The invention relates to a photoinduced cell covalent labeled fluorescent molecule, and a preparation method and application thereof, in particular to a compound as shown in the following formula (I), or a tautomer, an enantiomer, an enantiomer, a diastereomer, a racemate, a precursor compound, an isotope compound and various forms of salts or hydrates thereof. The compounds can be used for preparing light-induced covalent labeled fluorescent probes and are used for positioning and imaging subcellular organelles in cell biology. The light-induced covalent labeled fluorescent probe has important application potential and outstanding practical value in cell biology research, proteomics research and dynamic change research of biomacromolecules.

The invention discloses a cyanine compound and a preparation method and an application thereof. The compound has a structure as shown in the general formula I. The probe is especially sensitive to environmental viscosity and can be used in detection of fluids and biotic environmental viscosity. The probe can be positioned at different subcellular organelles and can monitor viscosity changes in subcellular organelles in living cells by a bimodal detection means of fluorescence ratio and fluorescence lifetime.

The invention provides a fluorescent probe with functions of detecting hydrogen peroxide and performing photodynamic killing on cancer cell activity as well as a preparation method and application thereof, and belongs to the technical field of a biochemical material. The fluorescent probe with the functions of detecting hydrogen peroxide and performing photodynamic killing on cancer cell activityhas aggregation induced luminescence characteristic, can specifically respond to the hydrogen peroxide, generates a substance with stronger fluorescence, can be applied to detection on the hydrogen peroxide in cells, and can selectively position subcellular organelle lipid droplets in the cells; the product obtained through action between the fluorescent probe and the hydrogen peroxide has large stokes displacement, so self-absorption can be avoided and background interference can be reduced; the fluorescent probe as well as the product obtained through the action between the fluorescent probeand the hydrogen peroxide have high light stability and photobleaching resistance, and are suitable for long-time tracing of the hydrogen peroxide in living cells or living bodies; meanwhile, the product obtained through the action between the fluorescent probe and the hydrogen peroxide has active oxygen generation capacity, can be applied to photodynamic killing of the cancer cells and can construct a diagnosis and treatment reagent integrating diagnosis and treatment functions.