32results about How to "Mature and simple" patented technology

The invention provides a high-looseness tobacco sheet applied to a low-temperature cigarette and a production process. The tobacco sheet comprises, by mass, 50-80% of tobacco raw material powder, 10-30% of atomizing agent, 0.5-10% of adhesive, 0.01-1% of leavening agent, 5-15% of tobacco essence perfume and 0-30% of water and is manufactured by means of a roll pressing production process. During manufacturing of the sheet, the production process is not changed, only the leavening agent is added to a raw material formula to loosen the sheet, and absorption of a large quantity of the atomizing agent and the external perfume is facilitated. When a consumer smokes the low-temperature cigarette prepared from the sheet, the obtained smoke amount is larger, and the obtained physiological satisfaction feeling is stronger. At the same time, the production process of the sheet is mature, simple and high in controllability, and the quality is stable.

The invention provides a fluorescent probe, which contains a, response polypeptide and b, optical active peptide, wherein the optical active peptide is inserted into the response polypeptide. The invention also provides a nucleotide sequence or a complementary sequence for coding the fluorescent probe of any embodiment. The invention also provides a nucleotide sequence or complementary sequence expression vector in operational connection with an expression control sequence. The invention also provides a cell containing the expression vector. The invention also provides a method for preparing the fluorescent probe. The method comprises the steps of providing the cell containing the vector for expressing the fluorescent probe; culturing the cell under the cell expression condition; separating the fluorescent probe. The invention also provides the fluorescent probe or application of the fluorescent probe prepared by the method to branched chain amino acid detection. In one embodiment, thebranched chain amino acid is selected from aminocarproic acid, isoleucine and valine. The detection can be performed in vivo, in vitro, subcellular fraction, or in-situ level. The invention also provides a kit, which contains the fluorescent probe or the fluorescent probe prepared by the method.

The present invention provides a gene encoding hydrogen peroxide fluorescent probe, a preparation method and applications thereof, wherein the gene encoding hydrogen peroxide fluorescent probe comprises a H2O2-sensitive polypeptide OxyR and a fluorescent protein cpYFP showing through H2O2, and the fluorescent protein cpYFP is inserted into the H2O2-sensitive polypeptide OxyR to form the fluorescent probe. The gene encoding hydrogen peroxide fluorescent probe is characterized in that the gene encoding hydrogen peroxide fluorescent probe is the mutant obtained by carrying out mutation on the sequence of the fluorescent protein cpYFP in the fluorescent probe. According to the present invention, the gene encoding hydrogen peroxide fluorescent probe can detect H2O2 at a live bacterial level and a subcellular level, and has good specificity and H2O2 selectivity, and the gene encoding hydrogen peroxide fluorescent probe on the H2O2 dynamic response change is not affected by the introduction of the mutation site.

The invention relates to an amino acid optical probe, and a preparation method and an application thereof. On one hand, the invention relates to an optical probe comprising an amino acid sensitive polypeptide or a functional variant thereof and an optical active polypeptide or a functional variant thereof, wherein the optical active polypeptide or the functional variant thereof is located in a sequence of the amino acid sensitive polypeptide or the functional variant thereof. The invention also relates to the preparation method of the probe and an application of the probe in detection of aminoacids. The amino acid optical probe provided by the invention has relatively small molecular weight, has the advantages of easy maturation, large fluorescence dynamic change and good specificity, canbe expressed in different subcellular organelles of cells, and can detect amino acids inside and outside cells in high flux and quantitatively.

The present invention relates to a transcription factor gene that plays a key role in Solanaceae fruit ripening. Plants overexpressing the gene have fruits with deeper pigmentation and ripen more rapidly than controls. The invention also relates to transgenic plants comprising said gene, and methods of making said plants.

The invention relates to a proline optical probe and a preparing method and application thereof. On one hand, the optical probe comprises a proline sensitive polypeptide or a functional variant thereof and an optical activity polypeptide or a functional variant thereof, wherein the optical activity polypeptide or the functional variant thereof is located in a sequence of the proline sensitive polypeptide or the functional variant thereof. The invention further relates to the preparing method of the probe and application of the probe in detecting proline.

This invention relates to QTL responsible for significantly increased firmness in tomato fruit in the cultivated plant producing said tomato fruit, compared to fruit from a control tomato plant which does not have said genetic elements. A cultivated tomato plant producing tomato fruit with significantly increased fruit firmness and a method for detecting QTLs linked to significantly increased fruit firmness are also provided.

The invention provides DC cell for overexpression of TRAF6, a DC cell vaccine, a construction method and application, and belongs to the technical field of DC vaccines. According to the invention, theDC cell for overexpression of the TRAF6 is successfully constructed. The DC cell for overexpression of the TRAF6 is easier to maturate, can better present an antigen, stimulate immune response of T cell with stronger antigen specificity, enhance proliferation of CD4+T cells and induce Th1 type immune response, and can influence molecular expression of the downstream part of a TRAF6 signal path. The DC cell for overexpression of the TRAF6 can load a tumor antigen to obtain the DC cell vaccine. The DC cell vaccine provided by the invention has better tumor killing activity, can significantly improve the tumor inhibition rate, and can enhance the anti-tumor immune response, so that the DC cell vaccine can be used for preventing or treating adenocarcinoma, liver cancer, hematologic tumor andthe like, and brings good news to the majority of cancer patients.

The invention discloses a bitter bamboo dried noodles processing method. The method comprises the following steps: bitter bamboo powder preparation, burdening, dough kneading, slaking, calendering and strip cutting, drying, dough cutting, and packaging. The bitter bamboo preparation comprises the following processes: de-casing the bitter bamboo, slicing the material, performing vacuum drying, powdering the material, and sieving the material; and the burdening and dough kneading steps are characterized in that bitter bamboo powder, salt, and edible alkali are mixed in flour step by step, then water is added for dough kneading. According to the invention, bitter bamboo and the dried noodles are organically combined, the nutrition value and the local flavor of the dried noodles are enhanced, the production has the advantages of simplification, energy saving, labor reduction and equipment investment reduction, and the method is suitable for practical production.

The invention relates to a lactic acid optical probe and a preparation method and an application thereof. In one aspect, the present invention relates to an optical probe comprising a lactic acid-sensitive polypeptide or functional variant thereof and an optically active polypeptide or functional variant thereof wherein the optically active polypeptide or functional variant thereof is located within the sequence of the lactic acid-sensitive polypeptide or functional variant thereof. The invention also relates to a preparation method of the probe and an application of the probe in detection of lactic acid.

The invention relates to a food baking method and device. The method comprises the steps that A, raw sizing agents on a chain plate structure are conveyed into a baking oven through a conveying mechanism to be preheated; the preheating temperature ranges from 50 DEG C to 200 DEG C, and the preheating time ranges from 1 min to 5 min; B, the preheated raw sizing agents are stirred and defoamed, and the stirring time ranges from 10 s to 300 s; C, the defoamed raw sizing agents are baked and formed through the baking oven, the baking temperature ranges from 100 DEG C to 250 DEG C, and the time ranges from 20 min to 80 min. According to the food baking device, due to defoaming and steaming with a cover, food is more easily baked thoroughly, the internal tissue is more uniform, the expansion effect is better, and the best taste is achieved.

The invention provides a c-di-GMP fluorescent probe, which comprises (A) an optically active polypeptide or a variant having at least 70% sequence identity and optical activity with the optically active polypeptide, and (B) a c-di-GMP sensitive polypeptide or a variant having at least 70% sequence identity and c-di-GMP sensitivity with the c-di-GMP sensitive polypeptide, where A is located within the sequence of B, which divides B into two parts B1 and B2, a structure with B1-A-B2 in the direction from the amino terminal to the carboxyl terminal is formed, or one or more A is located between two or more B and is connected with the B at the two ends through a connector. The c-di-GMP fluorescent probe provided by the invention is relatively small in protein molecular weight, easy to express, large in fluorescence dynamic change and good in specificity, related application research can be carried out in cells and escherichia coli through gene operation, and high-throughput, implementation and quantitative detection of c-di-GMP can be realized.