32results about How to "Mature and simple" patented technology

The present invention provides a gene encoding hydrogen peroxide fluorescent probe, a preparation method and applications thereof, wherein the gene encoding hydrogen peroxide fluorescent probe comprises a H2O2-sensitive polypeptide OxyR and a fluorescent protein cpYFP showing through H2O2, and the fluorescent protein cpYFP is inserted into the H2O2-sensitive polypeptide OxyR to form the fluorescent probe. The gene encoding hydrogen peroxide fluorescent probe is characterized in that the gene encoding hydrogen peroxide fluorescent probe is the mutant obtained by carrying out mutation on the sequence of the fluorescent protein cpYFP in the fluorescent probe. According to the present invention, the gene encoding hydrogen peroxide fluorescent probe can detect H2O2 at a live bacterial level and a subcellular level, and has good specificity and H2O2 selectivity, and the gene encoding hydrogen peroxide fluorescent probe on the H2O2 dynamic response change is not affected by the introduction of the mutation site.

The invention relates to a glutamine optical probe and its preparation method and application. In one aspect, the present invention relates to an optical probe comprising a glutamine-sensitive polypeptide or a functional variant thereof and an optically active polypeptide or a functional variant thereof, wherein the optically active polypeptide or a functional variant thereof is positioned at the glutamine-sensitive polypeptide or a functional variant thereof Within the sequence of the functional variant. The present invention also relates to the preparation method of the above-mentioned probe and its application in detecting amino acids. The glutamine optical probe provided by the invention has a relatively small molecular weight and is easy to mature, has large dynamic changes in fluorescence and good specificity, can be expressed in different subcellular organelles of cells, and can detect amino acids in a high-throughput and quantitative manner inside and outside cells.

The invention discloses a novel method for manufacturing a chip diode. The method comprises the following steps of: welding two copper leading wire electrodes of the diode, a soldering lug and a diode chip; pickling; washing by using high purity water; washing by using absolute ethanol; drying; coating; injecting; and processing a pin to form a finished product. The method is characterized in that: in the step of pickling, pickling is performed by adding acid liquor at a fixed point; in the step of washing by using the high purity water, the diode is washed by spraying water flow in a fan-shaped manner. According to the method, a mode of acid addition during pickling is changed, the traditional scattered mode is changed into the method of acid liquor addition at the fixed point, the acid consumption is reduced, the pollution to the environment is reduced, the corrosion to copper leading wires is reduced; and the high temperature performance of a product is greatly improved during washing, and the production process is simple and mature, high in efficiency, and low in energy consumption.

The invention provides an electric heating frying pan with an automatic vegetable cutting device, which includes a base, a heating furnace, a pot body, a mounting part and a vegetable cutting machine. The middle part of the base is disc-shaped, and one end of the base is "L" , the other end is arc-shaped and extends upwards, and one end of the base is installed with a panel for controlling the heating furnace; The eddy current furnace body has a circuit board installed inside the furnace body, a coil is arranged on the circuit board, and the panel is connected to the circuit board through wires; the pot body is placed above the furnace body, and the partition plate divides the pot body into two grids; The vegetable cutting machine includes a housing, a feeding port, a motor, a cover plate, a cutter head, a switch and a feeding port, and the inside of the housing is a hollow vegetable cutting chamber. The invention has a novel structure and a reasonable design, and integrates a frying pan, a vegetable cutter and an electric heating furnace into one body, which not only saves space, but also is easy to manage and use, and improves work efficiency.

The invention relates to a method for preparing high purity magnesium oxide from old brine as a raw material and producing hydrochloric acid as a by-product. The method comprises the following steps of eliminating a color and SO4<2-> of old brine, carrying out evaporation crystallization on the solution to obtain magnesium chloride crystals, washing the crystals by a high purity magnesium chloride saturated liquid, carrying out fluid pyrolysis on magnesium chloride to obtain magnesium oxide, simultaneously carrying out tail gas absorption to obtain hydrochloric acid as a by-product, carrying out water washing and carrying out magnesium oxide roasting to obtain high purity magnesium oxide. Through multiple purification processes such as impurity removal, crystallization, washing, pyrolysis and water washing, the high purity magnesium oxide with magnesium oxide purity of 99% or more is obtained.

The invention discloses a box carrying clamp. The box carrying clamp is characterized by comprising a handle, an upper shell, an upper ratchet wheel, an upper cover, a reset spring, a lower ratchet wheel, a lower shell, a push rod, a main spring, a transverse rod, a left clamping assembly and a right clamping assembly. According to the box carrying clamp, through a ratchet wheel mechanism and a connecting rod mechanism, conversion from vertical displacement to horizontal displacement is achieved, and through the ingenious design of the ratchet wheel mechanism, the whole mechanism has self-locking performance; moreover, through the design, during carrying, the weight of goods is mainly borne on the shells of the clamp, thus the internal movement mechanism is stressed little, and the size and weight of the internal mechanism are reduced; meanwhile, the ratchet wheel mechanism can well adapt to repetition in the carrying process, the using frequency is high, the connecting rod mechanism is compact in structure, mature and simple, parts are easily machined and conveniently replaced, and the economical performance and reliability are greatly improved.

The invention provides a gene encoded nicotinamide adenine dinucleotide phosphate (NADPH) fluorescent probe, a preparation method and application thereof. The gene encoded NADPH fluorescent probe includes: polypeptide B sensitive to NADPH and fluorescent protein A for expression of NADPH. The polypeptide B sensitive to NADPH interacts with NADPH to cause the fluorescence intensity change of fluorescent protein A. The NADPH fluorescent probe can detect NADPH in vivo, in vitro, at subcellular or in-situ level. The probe has very good specificity, has no response to ATP and other analogues, and also has no interference to NADH and other analogues. The probe protein is relatively small and easy to mature, and the fluorescence dynamic change is great, the probe is a technology suitable for physiological level and subcellular specific real-time detection of NADPH.

The invention relates to a food baking method and device. The method comprises the steps that A, raw sizing agents on a chain plate structure are conveyed into a baking oven through a conveying mechanism to be preheated; the preheating temperature ranges from 50 DEG C to 200 DEG C, and the preheating time ranges from 1 min to 5 min; B, the preheated raw sizing agents are stirred and defoamed, and the stirring time ranges from 10 s to 300 s; C, the defoamed raw sizing agents are baked and formed through the baking oven, the baking temperature ranges from 100 DEG C to 250 DEG C, and the time ranges from 20 min to 80 min. According to the food baking device, due to defoaming and steaming with a cover, food is more easily baked thoroughly, the internal tissue is more uniform, the expansion effect is better, and the best taste is achieved.