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32results about How to "Mature and simple" patented technology

High-looseness tobacco sheet applied to low-temperature cigarette and production process

The invention provides a high-looseness tobacco sheet applied to a low-temperature cigarette and a production process. The tobacco sheet comprises, by mass, 50-80% of tobacco raw material powder, 10-30% of atomizing agent, 0.5-10% of adhesive, 0.01-1% of leavening agent, 5-15% of tobacco essence perfume and 0-30% of water and is manufactured by means of a roll pressing production process. During manufacturing of the sheet, the production process is not changed, only the leavening agent is added to a raw material formula to loosen the sheet, and absorption of a large quantity of the atomizing agent and the external perfume is facilitated. When a consumer smokes the low-temperature cigarette prepared from the sheet, the obtained smoke amount is larger, and the obtained physiological satisfaction feeling is stronger. At the same time, the production process of the sheet is mature, simple and high in controllability, and the quality is stable.
Owner:HUBEI CHINA TOBACCO IND

Branched chain amino acid fluorescent probe and application thereof

PendingCN108395484AMature and simpleLarge dynamic changes in fluorescencePolypeptide with affinity tagBacteria peptidesFluorescenceIn vivo
The invention provides a fluorescent probe, which contains a, response polypeptide and b, optical active peptide, wherein the optical active peptide is inserted into the response polypeptide. The invention also provides a nucleotide sequence or a complementary sequence for coding the fluorescent probe of any embodiment. The invention also provides a nucleotide sequence or complementary sequence expression vector in operational connection with an expression control sequence. The invention also provides a cell containing the expression vector. The invention also provides a method for preparing the fluorescent probe. The method comprises the steps of providing the cell containing the vector for expressing the fluorescent probe; culturing the cell under the cell expression condition; separating the fluorescent probe. The invention also provides the fluorescent probe or application of the fluorescent probe prepared by the method to branched chain amino acid detection. In one embodiment, thebranched chain amino acid is selected from aminocarproic acid, isoleucine and valine. The detection can be performed in vivo, in vitro, subcellular fraction, or in-situ level. The invention also provides a kit, which contains the fluorescent probe or the fluorescent probe prepared by the method.
Owner:EAST CHINA UNIV OF SCI & TECH

Histidine fluorescent probe as well as preparation method and application thereof

ActiveCN106905418AMature and simpleLarge dynamic changes in fluorescenceDepsipeptidesFluorescence/phosphorescenceFluorescenceChemistry
The invention provides a histidine fluorescent probe. The histidine fluorescent probe comprises polypeptide B sensitive to histidine and fluorescent protein A performing expression on histidine, wherein the fluorescent protein A in inserted into the polypeptide B to divide the polypeptide B into two parts of B1 and B2, and a B1-A-B2 type probe structure is formed; the polypeptide B and the histidine interact to enable fluorescent signals of the fluorescent protein A to be changed; and the polypeptide B is HBP and a mutant thereof. The histidine fluorescent probe provided by the invention is relatively small in albumen molecular weight, is easy to mature, large in fluorescent dynamic changes and good in specificity, and can be expressed in different subcells of cells by a gene manipulation method, and histidine can be detected inside and outside the cells in a high-flux and quantitative manner.
Owner:EAST CHINA UNIV OF SCI & TECH

Breeding method of high-yield multi-resistance non-glutinous rice suitable for light simplified cultivation

The invention discloses a breeding method of high-yield multi-resistance non-glutinous rice suitable for light simplified cultivation, which belongs to a breeding method of plant seeds. The method comprises the steps of generation segregation through early sowing at an early stage, screening through late sowing, high-density direct sowing, low-nitrogenous-fertilizer-level planting, direct sowing and mixed harvesting to obtain seeds which satisfy requirements. The rice bred through the method has the characteristics that the rice is suitable for light simplified cultivation, is high-yield and is multi-resistant. Under the transplanting condition of individual plants, the growth situation is good, the self-regulating capability is strong, the stem is robust, the rice is fertilizer-resistant and anti-falling, the rice can be directly planted in the early ten days and middle ten days of June, the spike length is generally and approximately 18cm, the number of productive spikes per mu can reach more than 200 thousand, the total grain number per spike is 130-140, the fruiting rate is above 90 percent, the weight per thousand grains is 27-28g, the rice grains are full, the commodity value is good and the processing quality is good.
Owner:江苏丰源种业有限公司

Novel method for manufacturing chip diode

The invention discloses a novel method for manufacturing a chip diode. The method comprises the following steps of: welding two copper leading wire electrodes of the diode, a soldering lug and a diode chip; pickling; washing by using high purity water; washing by using absolute ethanol; drying; coating; injecting; and processing a pin to form a finished product. The method is characterized in that: in the step of pickling, pickling is performed by adding acid liquor at a fixed point; in the step of washing by using the high purity water, the diode is washed by spraying water flow in a fan-shaped manner. According to the method, a mode of acid addition during pickling is changed, the traditional scattered mode is changed into the method of acid liquor addition at the fixed point, the acid consumption is reduced, the pollution to the environment is reduced, the corrosion to copper leading wires is reduced; and the high temperature performance of a product is greatly improved during washing, and the production process is simple and mature, high in efficiency, and low in energy consumption.
Owner:RUGAO EADA ELECTRONICS

Gene encoding hydrogen peroxide fluorescent probe, preparation method and applications thereof

The present invention provides a gene encoding hydrogen peroxide fluorescent probe, a preparation method and applications thereof, wherein the gene encoding hydrogen peroxide fluorescent probe comprises a H2O2-sensitive polypeptide OxyR and a fluorescent protein cpYFP showing through H2O2, and the fluorescent protein cpYFP is inserted into the H2O2-sensitive polypeptide OxyR to form the fluorescent probe. The gene encoding hydrogen peroxide fluorescent probe is characterized in that the gene encoding hydrogen peroxide fluorescent probe is the mutant obtained by carrying out mutation on the sequence of the fluorescent protein cpYFP in the fluorescent probe. According to the present invention, the gene encoding hydrogen peroxide fluorescent probe can detect H2O2 at a live bacterial level and a subcellular level, and has good specificity and H2O2 selectivity, and the gene encoding hydrogen peroxide fluorescent probe on the H2O2 dynamic response change is not affected by the introduction of the mutation site.
Owner:EAST CHINA UNIV OF SCI & TECH

Amino acid optical probe, and preparation method and application thereof

The invention relates to an amino acid optical probe, and a preparation method and an application thereof. On one hand, the invention relates to an optical probe comprising an amino acid sensitive polypeptide or a functional variant thereof and an optical active polypeptide or a functional variant thereof, wherein the optical active polypeptide or the functional variant thereof is located in a sequence of the amino acid sensitive polypeptide or the functional variant thereof. The invention also relates to the preparation method of the probe and an application of the probe in detection of aminoacids. The amino acid optical probe provided by the invention has relatively small molecular weight, has the advantages of easy maturation, large fluorescence dynamic change and good specificity, canbe expressed in different subcellular organelles of cells, and can detect amino acids inside and outside cells in high flux and quantitatively.
Owner:EAST CHINA UNIV OF SCI & TECH

Modulation of solanaceae fruit ripening

PendingUS20140137296A1Enhanced fruit ripening propertySlow ripening propertySugar derivativesMicrobiological testing/measurementRipeningPigmentations
The present invention relates to a transcription factor gene that plays a key role in Solanaceae fruit ripening. Plants overexpressing the gene have fruits with deeper pigmentation and ripen more rapidly than controls. The invention also relates to transgenic plants comprising said gene, and methods of making said plants.
Owner:SYNGENTA PARTICIPATIONS AG

Proline optical probe and preparing method and application thereof

ActiveCN109666068AMature and simpleLarge dynamic changes in fluorescenceBacteria peptidesFluorescence/phosphorescenceProlineChemistry
The invention relates to a proline optical probe and a preparing method and application thereof. On one hand, the optical probe comprises a proline sensitive polypeptide or a functional variant thereof and an optical activity polypeptide or a functional variant thereof, wherein the optical activity polypeptide or the functional variant thereof is located in a sequence of the proline sensitive polypeptide or the functional variant thereof. The invention further relates to the preparing method of the probe and application of the probe in detecting proline.
Owner:EAST CHINA UNIV OF SCI & TECH

QTL Responsible for Tomato Fruit Firmness

This invention relates to QTL responsible for significantly increased firmness in tomato fruit in the cultivated plant producing said tomato fruit, compared to fruit from a control tomato plant which does not have said genetic elements. A cultivated tomato plant producing tomato fruit with significantly increased fruit firmness and a method for detecting QTLs linked to significantly increased fruit firmness are also provided.
Owner:SYNGENTA PARTICIPATIONS AG

DC cell for overexpression of TRAF6, DC cell vaccine, construction method and application

The invention provides DC cell for overexpression of TRAF6, a DC cell vaccine, a construction method and application, and belongs to the technical field of DC vaccines. According to the invention, theDC cell for overexpression of the TRAF6 is successfully constructed. The DC cell for overexpression of the TRAF6 is easier to maturate, can better present an antigen, stimulate immune response of T cell with stronger antigen specificity, enhance proliferation of CD4+T cells and induce Th1 type immune response, and can influence molecular expression of the downstream part of a TRAF6 signal path. The DC cell for overexpression of the TRAF6 can load a tumor antigen to obtain the DC cell vaccine. The DC cell vaccine provided by the invention has better tumor killing activity, can significantly improve the tumor inhibition rate, and can enhance the anti-tumor immune response, so that the DC cell vaccine can be used for preventing or treating adenocarcinoma, liver cancer, hematologic tumor andthe like, and brings good news to the majority of cancer patients.
Owner:康九生物科技(长春)有限公司

Alpha-ketoglutaric acid optical probe and preparation method and application thereof

The invention provides an optical probe, which comprises a) a response polypeptide, and b) an optically active polypeptide, wherein the optically active polypeptide is inserted into the response polypeptide. The invention provides a nucleic acid sequence encoding the optical probe according to any embodiment of the invention or a complementary sequence thereof, provides an expression vector comprising the nucleic acid sequence operably linked to an expression control sequence or the complementary sequence thereof, provides cells comprising the expression vectors, and provides a method of preparing the optical probe. The method of preparing the optical probe comprises the following steps of: providing the cell comprising a vector expressing the optical probe, culturing the cell under conditions of expression of the cell, and isolating the optical probe. The invention provides the optical probe and the application of the optical probe prepared by the method disclosed by the invention inthe detection of alpha-ketoglutaric acid. The invention also provides a kit, which comprises the optical probe of the invention or the optical probe prepared by the method of the invention.
Owner:EAST CHINA UNIV OF SCI & TECH

Networked motor, remote data acquisition therefor, and fault diagnosis system therefor

A networked motor, including a motor body, including a stator, a rotor, and a housing, and a motor controller, including a power circuit, a digital controller, a drive module, and a detecting circuit. A communication circuit module is connected to the digital controller. The communication circuit module includes a wired communication module or a wireless communication module. The wired communication module or the wireless communication module is disposed in or outside the motor controller.
Owner:ZHONGSHAN BROAD OCEAN MOTOR MFG

Bitter bamboo dried noodles processing method

The invention discloses a bitter bamboo dried noodles processing method. The method comprises the following steps: bitter bamboo powder preparation, burdening, dough kneading, slaking, calendering and strip cutting, drying, dough cutting, and packaging. The bitter bamboo preparation comprises the following processes: de-casing the bitter bamboo, slicing the material, performing vacuum drying, powdering the material, and sieving the material; and the burdening and dough kneading steps are characterized in that bitter bamboo powder, salt, and edible alkali are mixed in flour step by step, then water is added for dough kneading. According to the invention, bitter bamboo and the dried noodles are organically combined, the nutrition value and the local flavor of the dried noodles are enhanced, the production has the advantages of simplification, energy saving, labor reduction and equipment investment reduction, and the method is suitable for practical production.
Owner:LESHAN NORMAL UNIV

Arginine fluorescent probe and preparation method and application thereof

The invention provides an arginine fluorescent probe. The arginine fluorescent probe comprises polypeptide B responding to arginine and fluorescent protein A expressing the arginine; the fluorescent protein A is inserted into the polypeptide B, the polypeptide B is divided into two upper and lower structural parts of B1 and B2, and a B1-A-B2 type probe structure is formed; similarly, truncated and site-specific mutagenesis optimized mutants are located at different positions, and the polypeptide B and the arginine are specifically bound to cause change of a fluorescent signal of the fluorescent protein A; and the polypeptide B is arginine binding protein and a mutant thereof. The arginine fluorescent probe is relatively small in protein molecular weight, easy to express, large in fluorescent dynamic change and good in specificity, can be expressed in different subcellular organelles of cells through gene operation, and can quantitatively detect the arginine in and out of the cells in a high-throughput mode.
Owner:EAST CHINA UNIV OF SCI & TECH

Lactic acid optical probe as well as preparation method and application thereof

The invention relates to a lactic acid optical probe and a preparation method and an application thereof. In one aspect, the present invention relates to an optical probe comprising a lactic acid-sensitive polypeptide or functional variant thereof and an optically active polypeptide or functional variant thereof wherein the optically active polypeptide or functional variant thereof is located within the sequence of the lactic acid-sensitive polypeptide or functional variant thereof. The invention also relates to a preparation method of the probe and an application of the probe in detection of lactic acid.
Owner:EAST CHINA UNIV OF SCI & TECH

Tryptophan optical probe as well as preparation method and application thereof

The invention relates to a tryptophan optical probe as well as a preparation method and application thereof. In one aspect, the invention relates to an optical probe. The optical probe comprises a tryptophan-sensitive polypeptide or functional variant thereof and an optically active polypeptide or functional variant thereof, wherein the optically active polypeptide or functional variant thereof is located within the sequence of the tryptophan-sensitive polypeptide or functional variant thereof. The invention also relates to a preparation method of the probe and application of the probe in tryptophan detection.
Owner:EAST CHINA UNIV OF SCI & TECH

Novel method for manufacturing chip diode

The invention discloses a novel method for manufacturing a chip diode. The method comprises the following steps of: welding two copper leading wire electrodes of the diode, a soldering lug and a diode chip; pickling; washing by using high purity water; washing by using absolute ethanol; drying; coating; injecting; and processing a pin to form a finished product. The method is characterized in that: in the step of pickling, pickling is performed by adding acid liquor at a fixed point; in the step of washing by using the high purity water, the diode is washed by spraying water flow in a fan-shaped manner. According to the method, a mode of acid addition during pickling is changed, the traditional scattered mode is changed into the method of acid liquor addition at the fixed point, the acid consumption is reduced, the pollution to the environment is reduced, the corrosion to copper leading wires is reduced; and the high temperature performance of a product is greatly improved during washing, and the production process is simple and mature, high in efficiency, and low in energy consumption.
Owner:RUGAO EADA ELECTRONICS

A method for preparing high-purity magnesium oxide from old brine

The invention relates to a method for preparing high purity magnesium oxide from old brine as a raw material and producing hydrochloric acid as a by-product. The method comprises the following steps of eliminating a color and SO4<2-> of old brine, carrying out evaporation crystallization on the solution to obtain magnesium chloride crystals, washing the crystals by a high purity magnesium chloride saturated liquid, carrying out fluid pyrolysis on magnesium chloride to obtain magnesium oxide, simultaneously carrying out tail gas absorption to obtain hydrochloric acid as a by-product, carrying out water washing and carrying out magnesium oxide roasting to obtain high purity magnesium oxide. Through multiple purification processes such as impurity removal, crystallization, washing, pyrolysis and water washing, the high purity magnesium oxide with magnesium oxide purity of 99% or more is obtained.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Food baking method and device

ActiveCN104522102AEliminate Bubble ProblemsPrevent external heat and internal coolingBaking ovenEngineering
The invention relates to a food baking method and device. The method comprises the steps that A, raw sizing agents on a chain plate structure are conveyed into a baking oven through a conveying mechanism to be preheated; the preheating temperature ranges from 50 DEG C to 200 DEG C, and the preheating time ranges from 1 min to 5 min; B, the preheated raw sizing agents are stirred and defoamed, and the stirring time ranges from 10 s to 300 s; C, the defoamed raw sizing agents are baked and formed through the baking oven, the baking temperature ranges from 100 DEG C to 250 DEG C, and the time ranges from 20 min to 80 min. According to the food baking device, due to defoaming and steaming with a cover, food is more easily baked thoroughly, the internal tissue is more uniform, the expansion effect is better, and the best taste is achieved.
Owner:GUANGZHOU FUYA MACHINERY

Cyclic diguanylate optical probe as well as preparation method and application thereof

The invention provides a c-di-GMP fluorescent probe, which comprises (A) an optically active polypeptide or a variant having at least 70% sequence identity and optical activity with the optically active polypeptide, and (B) a c-di-GMP sensitive polypeptide or a variant having at least 70% sequence identity and c-di-GMP sensitivity with the c-di-GMP sensitive polypeptide, where A is located within the sequence of B, which divides B into two parts B1 and B2, a structure with B1-A-B2 in the direction from the amino terminal to the carboxyl terminal is formed, or one or more A is located between two or more B and is connected with the B at the two ends through a connector. The c-di-GMP fluorescent probe provided by the invention is relatively small in protein molecular weight, easy to express, large in fluorescence dynamic change and good in specificity, related application research can be carried out in cells and escherichia coli through gene operation, and high-throughput, implementation and quantitative detection of c-di-GMP can be realized.
Owner:EAST CHINA UNIV OF SCI & TECH

Method for Tissue Culture Regeneration of Mature Bromegrass Seeds

The invention discloses a method for tissue culture and regeneration of mature seeds of awnless brome. The mature seeds of awnless brome are used as explants, and the surface is first sterilized, and then the sterilized seeds of awnless brome are transferred to cultured in the callus induction medium; the induced callus was transferred to the first subculture medium for the first subculture, and then transferred to the differentiation medium for culture; the adventitious buds obtained from the differentiation culture were inserted into the second The second subculture was carried out in the subculture medium, and after differentiation and emergence, the seedlings were inserted into the rooting medium to induce rooting. In this method, mature brome seeds are used as explants, and callus is induced, differentiated and rooted by adding different concentrations of auxin and cytokinin in the medium, so as to establish its complete tissue culture regeneration system. Wheat provides the basis for genetic improvement through genetic engineering.
Owner:GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI

Gene-encoded nicotinamide adenine dinucleotide phosphate fluorescent probe and its preparation method and application

ActiveCN104910276BMature and simpleLarge dynamic changes in fluorescenceBacteriaMicrobiological testing/measurementIn vivoGene
The invention provides a gene encoded nicotinamide adenine dinucleotide phosphate (NADPH) fluorescent probe, a preparation method and application thereof. The gene encoded NADPH fluorescent probe includes: polypeptide B sensitive to NADPH and fluorescent protein A for expression of NADPH. The polypeptide B sensitive to NADPH interacts with NADPH to cause the fluorescence intensity change of fluorescent protein A. The NADPH fluorescent probe can detect NADPH in vivo, in vitro, at subcellular or in-situ level. The probe has very good specificity, has no response to ATP and other analogues, and also has no interference to NADH and other analogues. The probe protein is relatively small and easy to mature, and the fluorescence dynamic change is great, the probe is a technology suitable for physiological level and subcellular specific real-time detection of NADPH.
Owner:EAST CHINA UNIV OF SCI & TECH

Pyruvate optical probe as well as preparation method and application thereof

PendingCN113336855AMature and simpleLarge dynamic changes in fluorescenceBiological testingHybrid peptidesPyruvic acidPhysics
The invention relates to a pyruvate optical probe as well as a preparation method and application thereof. In one aspect, the invention relates to an optical probe. The optical probe comprises a pyruvate-sensitive polypeptide and an optically active polypeptide, wherein the optically active polypeptide is located within the sequence of the pyruvate-sensitive polypeptide. The invention also relates to a preparation method of the probe and application of the probe in pyruvate detection.
Owner:EAST CHINA UNIV OF SCI & TECH

Proline optical probe and preparation method and application thereof

The present invention relates to a proline optical probe and its preparation method and application. In one aspect, the present invention relates to an optical probe comprising a proline-sensitive polypeptide or a functional variant thereof and an optically active polypeptide or a functional variant thereof, wherein the optically active polypeptide or a functional variant thereof is located in the proline-sensitive polypeptide or its functional variant within the sequence of functional variants. The present invention also relates to the preparation method of the above probe and its application in detecting proline.
Owner:EAST CHINA UNIV OF SCI & TECH
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