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Glutamine optical probe and its preparation method and application

A glutamine and optical probe technology, applied in the field of optical probes, can solve in situ, real-time, dynamic, high-throughput and high-temporal-resolution detection of non-viable cells and subcellular organelles, not suitable for living cell research, Cell fragmentation and other problems, to achieve the effect of eliminating sample processing steps, large dynamic changes in fluorescence, and good specificity

Active Publication Date: 2022-05-31
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

[0006] However, these detection methods in the prior art are not suitable for living cell research, and there are many defects: time-consuming sample processing procedures are required, such as cell disruption, separation, extraction and purification, etc.; Bit, real-time, dynamic, high-throughput and high-temporal-resolution detection

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  • Glutamine optical probe and its preparation method and application
  • Glutamine optical probe and its preparation method and application
  • Glutamine optical probe and its preparation method and application

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[0073] The term "nucleic acid" or "nucleotide" as used herein may be in the form of DNA or RNA. DNA forms include cDNA,

[0079] In the present invention, the optical probe is expressed intracellularly, on the cell membrane, or secreted outside the cell. If needed, it can be

[0081] Herein, concentrations, amounts, percentages and other numerical values ​​may be expressed in the form of ranges. It should also be understood that using

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Embodiment 2

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Abstract

The invention relates to a glutamine optical probe and its preparation method and application. In one aspect, the present invention relates to an optical probe comprising a glutamine-sensitive polypeptide or a functional variant thereof and an optically active polypeptide or a functional variant thereof, wherein the optically active polypeptide or a functional variant thereof is positioned at the glutamine-sensitive polypeptide or a functional variant thereof Within the sequence of the functional variant. The present invention also relates to the preparation method of the above-mentioned probe and its application in detecting amino acids. The glutamine optical probe provided by the invention has a relatively small molecular weight and is easy to mature, has large dynamic changes in fluorescence and good specificity, can be expressed in different subcellular organelles of cells, and can detect amino acids in a high-throughput and quantitative manner inside and outside cells.

Description

Glutamine optical probe and preparation method and application thereof technical field The present invention relates to optical probe technical field, relate in particular to a kind of glutamine optical probe and preparation method thereof and application. Background technique Amino acid is a compound in which the hydrogen atom on the carbon atom of the carboxylic acid is replaced by an amino group, and it is a compound containing a basic amino group and an acidic Carboxylic organic compounds. The amino acids obtained after proteolysis are all α-amino acids whose amino groups are connected to the α-carbon, and There are only two dozen species, and they are the basic units that make up proteins. Amino acids work through metabolism in the human body, such as synthesis tissue protein; synthesis of ammonia-containing substances such as acids, hormones, antibodies, creatine; converted into carbohydrates and fats; or oxidized into Carbon dioxide and water and urea, pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62G01N21/64
CPCG01N21/6428C07K14/195C07K2319/60G01N2021/6439
Inventor 杨弋赵玉政李写顾燕芳胡晗阳陈念
Owner EAST CHINA UNIV OF SCI & TECH
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