223 results about "Gln - Glutamine" patented technology

A method of producing a polypeptide in fed batch cell culture is provided which involves an initial cell growth phase and a distinct production phase. In the initial growth stage, animal cells having nucleic acid encoding the polypeptide are cultured at a starting osmolality of about 280-330 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. This is followed by a production phase, where the cultured animal cells of the growth phase are inoculated at a cell seed density of at least 1.0×10⁶ cells/mL and the cells are cultured at a starting osmolarity of about 400-600 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. Preferably, the glutamine concentration in the cell culture medium is simultaneously controlled in order to curtail production of lactic acid and ammonia which result from unnecessarily high glutamine concentrations. During the growth phase, production of potentially detrimental metabolic waste products, such as lactic acid, is controlled thereby curtailing the increase of osmolality due to accumulation and neutralization of waste products. Thus, the cell growth can be improved. In the production phase, the cell culture conditions are modified in order to arrest or reduce cell growth and thereby direct nutrient utilization toward production, as opposed to cell growth. Overall, it is intended that the method results in an improvement in specific productivity, reduction in production run times and/or an increase in final product concentration.

The invention provides a method of synthesizing a diketopiperazine of the formula:

wherein:

• • R¹ is —CH₂COR³, or —CH₂CH₂COR³;
  • R² is the side chain of an amino acid selected from the group consisting of glycine, alanine, valine, leucine, isoleucine, serine, threonine, aspartic acid, asparagine, glutamic acid, glutamine, lysine, hydroxylysine, histidine, arginine, phenylalanine, tyrosine, tryptophan, thyroxine, cysteine, methionine, norvaline and ornithine;
  • R³ is —OH, —NH₂, —OR⁴, —NHR⁴, or —NR⁴R⁴; and
  • each R⁴ is independently an alkyl, aryl, alkylaryl, or arylalkyl.

An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

The invention discloses a preparing method of N(2)-L-alanyl-L-glutamine, which comprises the following steps: producing precursor acid potassium; producing mixed acid anhydride; producing glutamine sodium salt; producing N(2)-L-alanyl-L-glutamine (ala paddy dipeptide); refining. This invention possesses the advantages of simple craft, convenient operation and little contamination.

The invention relates to a bovine oocyte in-vitro fertilization and embryo culture method, and a transport culture solution. On one hand, the transport culture solution comprises glycine, alanine, arginine hydrochloride, aspartic acid, cystine dihydrochloride, glutamic acid, L-glutamine, histidine, threonine, tryptophan, tyrosine, valine, ascorbic acid, biotin, choline chloride, calcium pantothenate, folic acid, menadione and the like. The bovine oocyte in-vitro fertilization and embryo culture method comprises the following steps: oocyte collection and in-vitro maturation, in-vitro fertilization, and embryo in-vitro culture and preservation. The method and the related transport culture solution have excellent technical effects as described in the specification.

In its several embodiments, this invention discloses a pharmaceutical formulation comprising at least one antineoplastic agent or a pharmaceutically acceptable salt thereof, and at least one dissolution enhancing agent sufficient to substantially dissolve said at least one antineoplastic agent in at least one aqueous diluent, wherein said dissolution enhancing agent is urea, L-histidine, L-threonine, L-asparagine, L-serine, L-glutamine or mixtures thereof; a lyophilized powder comprising said pharmaceutical formulation, and articles of manufacture thereof.

The invention relates to a composite biological preparation with an obvious chemical hepatic injury protecting function, which consists of the following raw materials in weight ratio: 15-18 parts of glycine, 12-15 parts of arginine, 15-18 parts of cysteine, 16-18 parts of glutamine, 7-10 parts of S-adenosylmethionine and 7-12 parts of alpha-lipoic acid. The composite biological preparation has favorable therapeutic action and prevention action to all hepatic injury caused by various reasons, and has the effective rate (effectual rate) of 98.6% and the cure rate of 76.8%.

Disclosed are pharmaceutical compositions and methods of treating hepatoma by administering to patients or other populations of cells agents that selectively inhibit the uptake of glutamine by hepatocarcinoma cells, causing the concomitant apoptosis of hepatocarcinoma cells. Preferred agents target the amino acid transporter B0 (ATB0) protein for inhibition. Disclosed are antisense polynucleotides that reduce the expression of ATB0 in hepatocarcinoma cells, causing those cells to reduce the uptake of glutamine and subsequently undergo selective apoptosis.

The invention discloses a glutamine transaminase with improved heat stability and application thereof. High-efficiency expression of microbial transglutaminase (MTG) in escherichia coli serves as a modification platform, a label facilitating improvement of heat stability is added at the C end of MTG maturase, preference is given to IGCIILT to obtain a mutant strain with good enzymatic property, and the heat stability is improved by 2.5 times and 3 times. Modified enzyme is suitable for industrial application, the production cost can be reduced, and the production efficiency can be improved.

The invention discloses mutant type Taq DNA polymerase, a nucleic acid extraction-free direct PCR amplification kit and an application of the mutant type Taq DNA polymerase. By mutating 709-site glutamic acid of wild Taq DNA polymerase into glutamine, the activity, tolerance and the like of the obtained mutant type Taq DNA polymerase are obviously improved. The mutant type Taq DNA polymerase onlyhas a site, so that the mutation sites are few, and the preparation is easy. Experiment results show that the mutant type Taq DNA polymerase can be applied to a nucleic acid extraction-free direct PCRamplification reaction system, and the nucleic acid extraction-free direct PCR amplification of samples such as blood, foods and soil which contain a large number of inhibiting factors can be realized.

The invention discloses a recombined meat adhesive and a preparation method and application thereof, belonging to the field of food processing. The recombined meat adhesive comprises glutamine transaminase, non-meat protein and dietary fiber, has the advantages of low production cost, good adherence effect, great adherence strength to meat slices or meat blocks, little melting loss and boiling loss, great recombined meat tenderness and good flakiness, can effectively ensure the adherence effect, improves the demand of people for dietary fibers and increases the added value of products. The recombined meat prepared by the recombined meat adhesive has the adhesive property higher than or equal to 70g*cm<-2>, decreases the hardness by 62.8 percent and reduces the shearing force by 22.9 percent; the prepared raw and fresh recombined meat or cooked recombined meat has no obvious color difference (p is larger than 0.05) from one-piece meat and has no obvious taste difference (p is larger than 0.05) from the one-piece meat by sensory test.

The invention discloses a heat-resisting and freeze-drying protector for a live vaccine and a preparation method thereof as well as a live newcastle disease vaccine adopting the freeze-drying protector. The heat-resisting and freeze-drying protector for the live vaccine comprises the following compositions in percentage by weight: 10-15 percent of trehalose, 1-2 percent of polyvinylpyrrolidone (PVP), 1-2 percent of alum, 1-2 percent of glutamine, 2-10 percent of sorbitol, 0.1-0.5 percent of potassium dihydrogen phosphate, 0.5-1 percent of vitamin C and the balance of water. Compared with the traditional vaccine, the heat-resisting and freeze-drying protector for the live vaccine has the advantages of good stability, long preservation time, low preservation condition, little activity damage on the live vaccine, good dissolubility, simple raw material and the like.

The invention discloses a method that is used for finishing wool fabrics by catalyzing a foreign protein through a transglutaminase and belongs to the filed of biotechnological application. The method implements proper chemical or protease preprocessing on wools, grafts and coats a gelatinum of the foreign protein or a caseinum on the surface of wool fibers under the catalysis of the transglutaminase, endues the wool fabrics with certain felt-proof property, and improves the physical and mechanical performance of the wool fabrics to a certain degree. Compared with the traditional felt-proof finishing methods of the wools, the method that is used for finishing the wool fabrics by catalyzing the foreign protein through the transglutaminase replaces traditional chemical finishing agents with bio-enzyme and biomacromolecule, has less fiber strength loss and is easier to control by comparing with pure protease processing, leads the biological modification of the wools to be possible, greatly lowers environmental pollution, reduces COD in discharged water and improves ecological environment; simultaneously the method has gentle action condition, avoids high-temperature baking, saves energy and improves the quality of fabric products.

The invention discloses hydrogel applicable to cell adhesion and a preparation method of the hydrogel. The hydrogel is formed by Ac-IIISLGK-NH2 and L-Glutamine (L-Gln) under the action of an enzymatic reaction, wherein the N-terminal of the Ac-IIISLGK-NH2 is acetylated, the C-terminal of the Ac-IIISLGK-NH2 is aminated, the mole ratio of the Ac-IIISLGK-NH2 to the L-Gln is 1:1, reaction temperature is 37-40 DEG C, and reaction time is 24-48 hours. The hydrogel has the advantages that the prepared hydrogel can allow cells to be well adhered to cell surfaces, is low in toxicity and good in biocompatibility, does not cause the non-specific immunity reaction of lymphocyte, is ideal nano tissue engineering material and is significant to human life health.

The invention discloses an antibacterial mildew-resistant finishing agent for wool caps. The antibacterial mildew-resistant finishing agent comprises, by weight, 2-10 parts of sodium cyanate, 2-10 parts of hydrogen peroxide, 10-35 parts of modified chitosan, 2-10 parts of eucommia extract, 3-9 parts of bamboo leaf extract, 1-5 parts of cortex dictamni extract, 2-10 parts of pomegranate peel extract, 3-16 parts of radix lithospermi extract, 3-18 parts of beta-cyclodextrin, 0.1-1.2 parts of transglutaminase, 1-5 parts of amino siloxane, 2-10 parts of citric acid and 2-4 parts of hydrophilic surfactant. The antibacterial mildew-resistant finishing agent can endow the wool caps with excellent antibacterial performance and mildew resistance, feltability of the wool caps is lowered, and the woolcaps are enabled to be soft in hand feel and gentle in gloss.

A method of treating a problem relating to tissue metabolism by administering to a patient in need a single dose of glutamine in a pharmaceutically acceptable carrier. Also provided is glutamine for the treatment of an injury relating to tissue metabolism and for preventing cardiac cell damage. A therapeutic composition for treating and preventing cellular metabolic injury, preventing cardiac cell damage, and increasing heat shock protein expression where in the therapeutic includes a single dose of glutamine in a pharmaceutically acceptable carrier is also provided. A method of increasing the expression of heat shock proteins by administering a single dose of glutamine in a pharmaceutically acceptable carrier to a location in need is further provided.

The invention discloses a fermentation production method of high-yield glutamine. The fermentation production method comprises the following steps: Step One, seed culturing; Step Two, inoculating a fermentation medium with the strain obtained in the Step One for culture; during the fermentation process, controlling the glucose content, temperature, pH, dissolved oxygen and other conditions; Step Three, after the fermentation, extracting and refining glutamine from the fermentation broth. According to the fermentation production method of high-yield glutamine, the raw material is low in cost and easy to realize through control; the acid yield is high through fermentation, and the acid yield can reach 79.8g/L on average.

Methods for site-specific modification of protein are provided. These methods modify proteins which have been labeled at a particular site by the reaction of a transglutaminase with a glutamine peptide sequence which has been engineered into the protein. The site-specific modification methods of the invention are useful for producing reagents useful in high throughput screening methods and in producing protein delivery vehicles for specifically targeting cellular and non-cellular targets. Also described are improved biotinylation reagents.

The invention belongs to the technical field of fermentation and discloses a method for extracting glutamine from fermentation liquor. The method comprises the following steps of: cooling and filtering glutamine fermentation liquor, concentrating, cooling and crystallizing the obtained filtrate, and separating to obtain a crystallized crude product; adding 30-60 weight percent of water for washing the crystal, and separating to obtain a crude product in which the crystal is washed; and adding water for dissolving, sequentially adding a cation exchange resin and an anion exchange resin for removing salt, and filtering to obtain desalted liquid; performing nanofiltration to obtain nanofiltration liquid, decolorizing and filtering to obtain decolorized solution, concentrating, cooling, crystallizing, separating and drying to obtain glutamine. According to the method, the crude product is washed and crystallized, most of impurities on the surface of the crystallized crude product are removed, and the amount of the desalted resin is reduced, so that the sewage quantity is reduced, the energy consumption is reduced, and the pollution is reduced; and meanwhile, the method of sequentially adding the cation exchange resin and the anion exchange resin into the feed liquid is used for desalting, the glutamine loss caused by long desalting period is greatly reduced, and the yield of the glutamine is improved.

The invention discloses a method of producing L-alanyl-L-glutamine from recombinant escherichia coli, wherein the method of producing L-alanyl-L-glutamine from recombinant escherichia coli is follows: in an aqueous solution with a determined pH value, acting on free L-glutamine and L-alanine methyl ester hydrochloride to generate L-alanyl-L-glutamine, recombining a gene segment of the protein with the amino-acid ester acyltransferase of the L-alanyl-L-glutamine into a carrier and transferring into host bacteria, thereby obtaining the host bacteria with the recombinant DNA and capable of strengthening the activity of an L-alanyl-L-glutamine biological synthesis system. The method comprises the following steps: (1) culturing a larger number of recombinant escherichia coli cells for expressing the amino-acid ester acyltransferase; (2) excessively expressing the amino-acid ester acyltransferase in the step (1); and (3) taking the amino-acid ester acyltransferase in the step (2) as a crude enzyme source, adding the crude enzyme source into a buffer solution containing L-glutamine and L-alanine methyl ester hydrochloride substrate amino acid to react, thereby realizing efficient production of the L-alanyl-L-glutamine.

The invention discloses a cyclodextrin glucosyltransferase and a preparation method thereof, and belongs to the field of genetic engineering and enzyme engineering. The glycosylation efficiency of pigment is improved by 23%, 44% and 32% respectively by replacing alanine (Ala) at the No. 156 site of CGTase of P. Macerans strain JFB05-01(CCTCC NO: M208063) with lysine (Lys), glutamine (Gln) and valine (Val). Those mutant enzymes are more beneficial for catalyzing and producing glycosylated genistein compared with wild type CGTase.

The invention relates to compound amino acid multi-vitamin liquid micro-emulsion, which comprises vitamin A, vitamin D3, vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin K3, folic acid, calcium pantothenate, nicotinic acid, serine, lysine, threonine, tyrosine, cysteine, glutamine, methionine, lactamine, bursin, immune globulin, poly IC, docosahexaenoic acid (DHA) and taurine. A component 1 is obtained by dissolving fat soluble ingredients in phospholipid; a component 2 is obtained by dissolving aqueous soluble ingredients in purified water; the component 1 is homogenated to be added into the component 2 so as to obtain a mixture; a component 3 is obtained by mixing bursin, immune globulin and poly IC and by adding thermal stabilizing agent; and when the temperature of the mixture is decreased to 40 DEG C to 50 DEG C, the component 3, xanthan gum and Arabic gum are added into the mixture to be uniformly mixed. The compound amino acid multi-dimensional liquid micro-emulsion can be rapidly dissolved in the water, high-energy substances can be rapidly absorbed, and the bioavailability is high; the stable system guarantees the validity of the multiple vitamins, amino acid and the like during the storage period; and the cost performance is high.

A mutant poly(3-hydroxyalkanoic acid) synthase comprising an amino acid sequence of (a) or (b) below. (a) A mutant amino acid sequence derived from SEQ ID No. 1, wherein at least one amino acid selected from the group consisting of glutamic acid at position 130, serine at position 325, serine at position 477 and glutamine at position 481 is substituted by another amino acid. (b) A mutant amino acid sequence derived from SEQ ID No. 1 wherein at least one amino acid selected from the group consisting of glutamic acid at position 130, serine at position 325, serine at position 477 and glutamine at position 481 is substituted by another amino acid, wherein at least one amino acid other than the amino acids at positions 130, 325, 477 and 481 is deleted, substituted and/or added, wherein the mutant amino acid sequence has the activity to biosynthesize biodegradable polyester.