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Fermentation production method of high-yield glutamine

A technology of glutamine and production method, which is applied in the field of high-yield glutamine fermentation production, can solve the problems of less L-glutamine, the difference in the acid production level of strains, etc., so as to improve acid production, reduce carbon frame loss, inhibit the Effects of glutamate synthase activity

Active Publication Date: 2015-03-11
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Glutamine is mainly produced by industrial microbial fermentation. At present, there are very few enterprises in my country that have truly industrialized the production of L-glutamine, and the level of acid production by strains is far behind that of advanced foreign countries.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A high-yield glutamine fermentation production method includes the following steps:

[0025] Step 1. Use Corynebacterium glutamicum YG-3131 for seed culture first, control the air volume and rotation speed during the 7-9h of the culture process to reduce the dissolved oxygen to 15-20%, and the culture is completed to obtain the strain for use;

[0026] Step 2. Inoculate the strain obtained in step 1 into the fermentation medium according to the inoculation amount of 10%. The fermentation medium contains 7% glucose, 1% corn steep liquor, and NH by mass percentage, except for water. 4 Cl2%, KH 2 PO 4 0.2%, MgSO 4 0.08%, KCl 0.1%, calculated per liter, each liter of fermentation medium also contains 1g / L of yeast powder, FeSO 4 20mg / L, CuSO 4 2mg / L and thiamine 1mg / L; the fermentation medium is also added with 0.1% by mass inhibitor citric acid, and an activator of glutamine synthetase, and an activator of glutamine synthetase By MnCl 2 And ZnSO 4 Composition, addition amount is...

Embodiment 2

[0031] A high-yield glutamine fermentation production method includes the following steps:

[0032] Step 1. Use Corynebacterium glutamicum YG-3131 for seed culture first, control the air volume and rotation speed during the 7-9h of the culture process to reduce the dissolved oxygen to 15-20%, and the culture is completed to obtain the strain for use;

[0033] Step 2: Inoculate the strain obtained in step 1 into the fermentation medium according to the 15% inoculum amount. The fermentation medium contains 3% glucose, 1% corn steep liquor, and NH by mass percentage. 4 Cl2%, KH 2 PO 4 0.2%, MgSO 4 0.2%, KCl 0.2%, calculated per liter, each liter of fermentation medium also contains yeast powder 1.5g / L, FeSO 4 10mg / L, CuSO 4 10mg / L and thiamine 10mg / L; the fermentation medium is also added with 0.2% by mass inhibitor citric acid, and glutamine synthetase activator, glutamine synthetase activator By MnCl 2 And ZnSO 4 Composition, addition amount is MnCl 2 150mg / L and ZnSO 4 10mg / L;

[003...

Embodiment 3

[0038] A high-yield glutamine fermentation production method includes the following steps:

[0039] Step 1. Use Corynebacterium glutamicum YG-3131 for seed culture first, control the air volume and rotation speed during the 7-9h of the culture process to reduce the dissolved oxygen to 15-20%, and the culture is completed to obtain the strain for use;

[0040] Step 2. Inoculate the strain obtained in step 1 into the fermentation medium according to the inoculum amount of 13%. The fermentation medium contains 5% glucose, 2% corn steep liquor, and NH in mass percentage. 4 Cl3%, KH 2 PO 4 0.3%, MgSO 4 0.1%, KCl0.15%, calculated per liter, each liter of fermentation medium also contains yeast powder 1.2g / L, FeSO 4 15mg / L, CuSO 4 6mg / L and thiamine 6mg / L; the fermentation medium is also added with 0.15% by weight inhibitor citric acid, and glutamine synthetase activator, glutamine synthetase activator By MnCl 2 And ZnSO 4 Composition, addition amount is MnCl 2 100mg / L and ZnSO 4 45mg / L;

...

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Abstract

The invention discloses a fermentation production method of high-yield glutamine. The fermentation production method comprises the following steps: Step One, seed culturing; Step Two, inoculating a fermentation medium with the strain obtained in the Step One for culture; during the fermentation process, controlling the glucose content, temperature, pH, dissolved oxygen and other conditions; Step Three, after the fermentation, extracting and refining glutamine from the fermentation broth. According to the fermentation production method of high-yield glutamine, the raw material is low in cost and easy to realize through control; the acid yield is high through fermentation, and the acid yield can reach 79.8g / L on average.

Description

technical field [0001] The invention belongs to the field of microbial fermentation production, and in particular relates to a high-yield glutamine fermentation production method. Background technique [0002] L-Glutamine is an essential amino acid for the human body. It has special effects such as improving human immunity and significantly promoting brain function and gastrointestinal function. At present, it has a wide range of applications and good development prospects in the fields of food health care, feed additives, medicine and health. [0003] Glutamine is mainly produced by industrial microbial fermentation. At present, there are very few enterprises in my country that have truly industrialized the production of L-glutamine, and the level of acid production by strains is far behind that of advanced foreign countries. Contents of the invention [0004] The object of the present invention is to address the deficiencies in the prior art, and provide a high-yield gl...

Claims

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Application Information

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IPC IPC(8): C12P13/14C12R1/15
Inventor 岳贵龙孙朝阳徐晓鹏
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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