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Method for promoting accumulation of salvia miltiorrhiza hairy root rosmarinic acid

A technique of rosmarinic acid and hairy roots, which is applied in the directions of horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of not using the bypass inhibitor salvianolic acid and the like

Inactive Publication Date: 2018-01-12
ZHEJIANG SCI-TECH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, as far as this field is concerned, there is no report on the use of bypass inhibitors to study the biosynthetic pathway of salvianolic acid, except for the attempt to use genes to inhibit the biosynthetic pathway of salvianolic acid

Method used

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  • Method for promoting accumulation of salvia miltiorrhiza hairy root rosmarinic acid
  • Method for promoting accumulation of salvia miltiorrhiza hairy root rosmarinic acid
  • Method for promoting accumulation of salvia miltiorrhiza hairy root rosmarinic acid

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Embodiment Construction

[0034] This example implements a method for promoting the accumulation of rosmarinic acid in the hairy roots of Salvia miltiorrhiza, inoculating the hairy roots of Salvia miltiorrhiza in 6,7-V liquid medium for subculture, adding NEM to it for bypass inhibition treatment, Continue to cultivate after processing.

[0035] Among them, in the bypass inhibition treatment, 0<NEM concentration≤10mg / L, and the treatment effect increases with the treatment time, the better the promotion effect. The specific analysis is as follows:

[0036] 1. Method

[0037] 1) Using the suspension culture method of salvia miltiorrhiza root culture, the hairy roots subcultured in 6,7-V medium were used as research materials, and 0.5 g of hairy roots with good growth and uniformity were taken and inoculated into In 100mL of 6,7-V liquid medium, culture on a constant temperature shaker. Set the conditions as follows: temperature 25° C., 110 rpm, dark cultivation.

[0038] 2) Set different gradient co...

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Abstract

The invention relates to a method for promoting the accumulation of salvia miltiorrhiza hairy root rosmarinic acid, and belongs to the field of plant tissue culture technique by using a plant growth regulator. The method comprises the following steps: inoculating salvia miltiorrhiza hairy root into a 6,7-V fluid nutrient medium for subculture, adding NEM into the 6,7-V fluid nutrient medium for bypass inhibition treatment, and carrying out continuous culture after treatment is completed, wherein in the bypass inhibition treatment, the addition concentration of the NEM is greater than 0 and smaller than or equal to 10 mg / L, and the treatment effect and the promotion effect are better along with the increase of treatment time. The method is applied to culture of effective constituents of salvia miltiorrhiza, the approach of lignin can be inhibited, and the biological accumulation of the salvia miltiorrhiza hairy root reaches the maximum.

Description

technical field [0001] The invention relates to a method for promoting the accumulation of rosmarinic acid in hairy roots of Salvia miltiorrhiza, and belongs to the technical field of plant tissue culture with plant growth regulators. Background technique [0002] Some important resources of clinical medicine come from secondary metabolites of plants. Therefore, many researchers focus on the study of plant secondary metabolites, clarify its mechanism, and make plant secondary metabolites be used in the pharmaceutical industry and the development of natural products. Salviamiltiorrhiza Bunge is one of the most important traditional medicinal materials in my country. The secondary metabolites of Salviamiltiorrhiza Bunge, tanshinone and salvianolic acid, have the functions of promoting blood circulation and removing blood stasis, clearing away heat and calming the nerves, nourishing blood and nourishing qi, and have been widely used in crowns. The prevention and treatment of he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郭万里张慕罄梁宗锁杜旭红向倩倩杨佳瑶杨宗岐
Owner ZHEJIANG SCI-TECH UNIV
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