Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for promoting the accumulation of rosmarinic acid in hairy roots of Salvia miltiorrhiza

A technology of rosmarinic acid and hairy roots, applied in the field of promoting the accumulation of rosmarinic acid in the hairy roots of Salvia miltiorrhiza, can solve the problems of not using the bypass inhibitor salvianolic acid

Inactive Publication Date: 2020-09-01
ZHEJIANG SCI-TECH UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as this field is concerned, there is no report on the use of bypass inhibitors to study the biosynthetic pathway of salvianolic acid, except for the attempt to use genes to inhibit the biosynthetic pathway of salvianolic acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for promoting the accumulation of rosmarinic acid in hairy roots of Salvia miltiorrhiza
  • A method for promoting the accumulation of rosmarinic acid in hairy roots of Salvia miltiorrhiza
  • A method for promoting the accumulation of rosmarinic acid in hairy roots of Salvia miltiorrhiza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] This example implements a method for promoting the accumulation of rosmarinic acid in the hairy roots of Salvia miltiorrhiza, inoculating the hairy roots of Salvia miltiorrhiza in 6,7-V liquid medium for subculture, adding NEM to it for bypass inhibition treatment, Continue to cultivate after processing.

[0035] Among them, in the bypass inhibition treatment, 0

[0036] 1. Method

[0037] 1) Using the suspension culture method of salvia miltiorrhiza root culture, the hairy roots subcultured in 6,7-V medium were used as research materials, and 0.5 g of hairy roots with good growth and uniformity were taken and inoculated into In 100mL of 6,7-V liquid medium, culture on a constant temperature shaker. Set the conditions as follows: temperature 25° C., 110 rpm, dark cultivation.

[0038] 2) Set different gradient co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for promoting the accumulation of salvia miltiorrhiza hairy root rosmarinic acid, and belongs to the field of plant tissue culture technique by using a plant growth regulator. The method comprises the following steps: inoculating salvia miltiorrhiza hairy root into a 6,7-V fluid nutrient medium for subculture, adding NEM into the 6,7-V fluid nutrient medium for bypass inhibition treatment, and carrying out continuous culture after treatment is completed, wherein in the bypass inhibition treatment, the addition concentration of the NEM is greater than 0 and smaller than or equal to 10 mg / L, and the treatment effect and the promotion effect are better along with the increase of treatment time. The method is applied to culture of effective constituents of salvia miltiorrhiza, the approach of lignin can be inhibited, and the biological accumulation of the salvia miltiorrhiza hairy root reaches the maximum.

Description

technical field [0001] The invention relates to a method for promoting the accumulation of rosmarinic acid in hairy roots of Salvia miltiorrhiza, and belongs to the technical field of plant tissue culture with plant growth regulators. Background technique [0002] Some important resources of clinical medicine come from secondary metabolites of plants. Therefore, many researchers focus on the study of plant secondary metabolites, clarify its mechanism, and make plant secondary metabolites be used in the pharmaceutical industry and the development of natural products. Salviamiltiorrhiza Bunge is one of the most important traditional medicinal materials in my country. The secondary metabolites of Salviamiltiorrhiza Bunge, tanshinone and salvianolic acid, have the functions of promoting blood circulation and removing blood stasis, clearing away heat and calming the nerves, nourishing blood and nourishing qi, and have been widely used in crowns. The prevention and treatment of he...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郭万里张慕罄梁宗锁杜旭红向倩倩杨佳瑶杨宗岐
Owner ZHEJIANG SCI-TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com